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Method for identifying plant variety

A kind of plant species and plant technology, applied in the field of plant identification, can solve the problems of inability to identify similarities, etc., and achieve the effect of simple and easy identification method, rapid identification, and small sample amount

Inactive Publication Date: 2018-10-19
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
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Problems solved by technology

However, this method cannot accurately identify the species of plants with high morphological similarities that are difficult to dist

Method used

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  • Method for identifying plant variety
  • Method for identifying plant variety
  • Method for identifying plant variety

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Experimental program
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[0050] Example

[0051] (1) Preparation of buffer:

[0052] Dissolve potassium dihydrogen phosphate and disodium hydrogen phosphate in water to prepare a phosphate buffer; the concentrations of potassium dihydrogen phosphate and sodium dihydrogen phosphate are both 0.1 mol / L;

[0053] Dissolve Tris-base in water to prepare Tris buffer; the concentration of Tris-base is 0.1mol / L;

[0054] Sodium acetate and acetic acid were dissolved in water to prepare an acetic acid buffer; the concentrations of sodium acetate and acetic acid were both 0.1 mol / L.

[0055] (2) Preparation of plant tissue homogenate:

[0056] Take 0.5g Chinese Lycoris (Lycorischinensis) leaf tissue, 0.5g yellow long tube Lycoris (Lycorislongitubavar. Lycorisstraminea (Lycorisstraminea) leaf tissues were cut into pieces with scissors, and the 5 leaf tissues were added to 2 mL of absolute ethanol (analytical grade) and ground into a homogenate to obtain tissue homogenates of 5 species of Lycorisstraminea.

[0057] (3) Graph...

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Abstract

The invention provides a method for identifying plant varieties. On the basis of electrochemical analysis, a plant leaf tissue extract is immobilized on the surface of an electrode, redox reactions (chemical signals) of an obtained modification electrode in different buffer solutions are converted into electric parameters, whether two samples have identical electrochemical information or not is compared, and thus multiple plants can be classified or identified. The method provided by the invention is simple and feasible, and plants with approximate morphologies or incomplete morphology properties can be rapidly identified.

Description

technical field [0001] The invention relates to the technical field of plant identification, in particular to a method for identifying plant species. Background technique [0002] Plant taxonomy is a basic subject that mainly studies the origin, kinship, and evolutionary development of different groups in the entire plant kingdom. That is to classify the complex plant kingdom until it is identified to species, and arrange them systematically so that people can understand and use plants. [0003] According to incomplete statistics, there are more than 500,000 species of plants living on the earth (about 250,000 species of seed plants). In the prior art, people usually judge with the naked eye which kind of a certain plant is based on some relevant technical knowledge in plant classification, or distinguish whether two kinds of plants are the same kind. However, this method cannot accurately identify the species of plants with high morphological similarities that are difficu...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N1/28
CPCG01N27/26G01N1/28
Inventor 郑玉红傅力张鹏翀庄维兵刘天宇鲍淳松
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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