74results about How to "Rapid and Sensitive Detection" patented technology

The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.

The subject invention provides a novel SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to each structurally-similar molecule in said family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.

Disclosed are methods and compositions for detecting variation in nucleic acids. The disclosed method compares the sequence of a nucleic acid of interest with the sequence of a reference nucleic acid to sensitively identify variations between the sequence of a nucleic acid of interest and the sequence of a reference nucleic acid. The disclosed method generally involves excision and replacement of selected nucleotides in nucleic acid strands hybridized to other strands. In the method, if the excised nucleotide was mismatched with the nucleotide in the other, hybridized strand, then the replacement nucleotide will not be mismatched. If the excised nucleotide was not mismatched with the nucleotide in the other, hybridized strand, then the excised nucleotide is not replaced. This difference allows detection of variation in the nucleic acid of interest. In some forms of the method, by replacing excised nucleotides with nuclease-resistant nucleotides, strands in which excised nucleotides are replaced will be resistant to nuclease digestion while strands in which excised nucleotides are not replaced will be sensitive to nuclease digestion. By exposing the hybridizing nucleic acids to nuclease following replacement of excised nucleotides, the strands in which excised nucleotides are not replaced can be destroyed by the nuclease while strands in which excised nucleotides are replaced can be preserved. The remaining strands can then be detected and whether the strand survived nuclease digestion can be noted. Strands that survive nuclease digestion are indicative of the presence of variation in the nucleic acid of interest.

The invention discloses a histamine detection method for a fluorescence resonance energy transfer biosensor based on magnetic nano-particle separation. The method comprises the following steps: respectively immobilizing histamine antibodies on surfaces of magnetic nano-particles, graphene quantum dots subjected to surface amino functionlization and gold nanoparticles in a covalent binding manner; and adding the histamine enriched and separated by the magnetic nano-particles into a mixed solution of the graphene quantum dots and the gold nanoparticles. Because the histamine on the magnetic nano-particles can be in specific binding with the surfaces of the graphene quantum dots and the gold nanoparticles modified by the histamine antibodies, the distance between the graphene quantum dots and the gold nanoparticles is narrowed, and under ultraviolet excitation of the wavelength of 320nm, due to fluorescence resonance energy transfer, fluorescence of the graphene quantum dots is quenched by the gold nanoparticles. The content of the histamine is determined by detecting the fluorescence signal intensity. The detection limit in the method reaches 24pM. According to the method disclosed by the invention, the histamine can be rapidly, sensitively and accurately detected.

The invention provides a pair of PCR primers used for authenticating pork, beef, mutton and products thereof. The nucleotide sequences of the primers are represented by SEQ ID No.1 and No.2. The invention also provides a kit comprising the primers, and a method for authenticating pork, beef, mutton and products thereof. The method provided by the invention is characterized in accurate result and high sensitivity. With the method provided by the invention, adulterate fresh meats in markets can be rapidly detected. Also, the method plays an important role in individual discriminating and origintracing researches. According to the authenticating method provided by the invention, only one pair of PCR primers is required for authenticating fresh pork, beef, and mutton, such that the authentication is simple and rapid. With the method, adulterate fresh meats in markets can be rapidly and sensitively detected, and great significance is provided for pig individual discriminating and origin tracing researches.