2results about How to "Rapid and Sensitive Detection" patented technology

The present application relates to the technical field of immunology and meat product adulteration meat source identification, and provides a hybridoma cell strain, an anti-fox skeletal muscle troponin monoclonal antibody produced by the hybridoma cell strain and application of the antibody, the preservation name of the hybridoma cell strain is: the hybridoma cell strain skTn-3A4 of the anti-fox skeletal muscle troponin monoclonal antibody, the preservation place is China Center for Type Culture Collection, the preservation date is October 10, 2023, and the preservation number is CCTCC NO: C2023302.The anti-fox skeletal muscle troponin monoclonal antibody skTn-3A4 provided by the present application can be used for preparing an enzyme-linked immunoassay kit, a colloidal gold chromatography test strip and an evanescent wave fluorescence immune sensor for detecting fox skeletal muscle troponin, so that the fox skeletal muscle troponin in food can be rapidly and sensitively detected.

The invention provides a hybridoma cell strain, an anti-dibutyl phthalate monoclonal antibody generated by the hybridoma cell strain and application of the anti-dibutyl phthalate monoclonal antibody, and belongs to the technical field of monoclonal antibody preparation, and the preservation number of the hybridoma cell strain DBP-2C7 for generating the anti-dibutyl phthalate monoclonal antibody is CCTCC NO: C2024274. The anti-dibutyl phthalate monoclonal antibody is high in titer, the subtype of the anti-dibutyl phthalate monoclonal antibody is IgG1, the half inhibition ratio IC50 of the anti-dibutyl phthalate monoclonal antibody to dibutyl phthalate is 24.3 [mu] g / L, the cross reaction rate of the anti-dibutyl phthalate monoclonal antibody to dibutyl phthalate is 100.00%, and the cross reaction rate of the anti-dibutyl phthalate monoclonal antibody to structural analogues is less than 1%. The anti-dibutyl phthalate monoclonal antibody generated by the hybridoma cell strain DBP-2C7 disclosed by the invention can achieve the purpose of rapidly and sensitively detecting dibutyl phthalate in food and environment.