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3 results about "Nuclease digestion" patented technology
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Nucleases for DNA & RNA Digestion Purification of proteins and specific nucleic acids often requires the digestion of DNA, RNA or both. Viscosity problems resulting from high DNA concentrations and enzymatic cell dissociation methods are often enhanced utilizing DNAse.
Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides employing hybridization stringency and / or nuclease digestion for removing failure sequences.
Owner:DNA SCRIPT SAS
LINEAR DNA WITH ENHANCED RESISTANCE AGAINST EXONUCLEASES AND METHODS FOR ITS PRODUCTION
UndeterminedES3047730R1DNA - Deoxyribonucleic acidAdaptor molecule
Linear DNA with improved resistance against exonucleases and methods for its production. The present invention relates to methods for producing a linear deoxyribonucleic acid (DNA) product (e.g., a closed linear DNA product) with improved resistance to nuclease digestion. The present invention relates to methods comprising the steps of: (a) contacting a double-stranded DNA molecule with an endonuclease, a ligase, and first and second terminal adaptor molecules and intermediate adaptor molecules to form a single contiguous aqueous volume; and (b) incubating the single contiguous aqueous volume to generate a linear DNA product (e.g., a closed linear DNA product). The present invention also relates to linear deoxyribonucleic acid (DNA) products (e.g., a closed linear DNA product) with improved resistance to nuclease digestion and uses thereof.
Owner:4BASEBIO UK LTD
Methods for producing DNA product
PCT designated stageWO2026115064A1Microbiological testing/measurementDNA - Deoxyribonucleic acidNucleotide
Owner:4BASEBIO UK LTD