91 results about "DNA synthesis" patented technology

The invention discloses a semantic intelligent enhanced DNA storage method for the Internet of Things, and belongs to the crossing field of artificial intelligence and biological information storage, the compression efficiency and robustness of DNA image storage are effectively improved through introduced semantic extraction and a multi-read screening mechanism of design, key information areas in images are extracted through semantic extraction, and the compression efficiency and robustness of DNA image storage are improved. The method has the advantages that redundant backgrounds are eliminated, coding lengths are reduced, high-quality sequences are selected from sequencing copies by the aid of scoring and sequence analysis through a multi-read screening mechanism, fault tolerance is enhanced, the method is applicable to efficient semantic storage in scenes of the internet of things, the problem of low storage efficiency due to excessive redundant information in original images in existing DNA image storage methods is solved, and the method is applicable to high-efficiency semantic storage in scenes of the internet of things. And a high bit error rate exists in the DNA synthesis and sequencing process, so that the image recovery quality is influenced.

The invention discloses a DNA synthesis method which comprises the following steps: processing an initial substrate according to a preset strategy to obtain a target substrate with a Mark pattern and a plurality of reaction sites, and adjusting the positions of the target substrate and an ink-jet printing device through the Mark pattern, so that the working directions of a reaction unit array and the device are consistent, and the subsequent operation accuracy is ensured; deprotecting the reaction sites to generate active groups; sequentially spraying a target monomer solution and an activator solution into the reaction sites of the preset area after deprotection treatment through an ink-jet printing device, and carrying out a coupling reaction with the active groups to obtain a mixed solution containing a nucleotide chain; carrying out cleaning, oxidation, capping and deprotection treatment on the mixed solution to obtain a single-layer base product; according to the target base sequence, determining a corresponding target monomer solution, and circularly carrying out a reaction to obtain a mixed solution containing a nucleotide chain; and obtaining the synthesized DNA according to the mixed solution. According to the method, high-throughput, high-quality and high-efficiency DNA synthesis can be realized.

The invention relates to a high-throughput DNA synthesis device based on ink-jet printing. The high-throughput DNA synthesis device comprises a plurality of ink boxes, a sealing assembly, a lifting assembly, a bearing jig and a moving assembly. Each ink box is arranged at the corresponding ink jet station and is used for temporarily storing the DNA basic group reagent and jetting the DNA basic group reagent through an ink jet port, and the ink jet port can automatically control the volume of liquid drops. The bearing jig is used for bearing the reaction pore plate. And the plurality of sealing assemblies are respectively arranged at the reaction stations and are used for being in sealing fit with the bearing jig to form a closed reaction cavity. The multiple lifting assemblies are arranged on the connection stations below the reaction station and used for driving the bearing jig to rise, and the reaction pore plate is matched with the sealing assembly. The moving assembly is used for driving the bearing jig to move between the ink jet station and the connection station. According to the invention, the parallel operation of reagent ink jet, closed reaction and reaction and sample adding is realized, the liquid drop control precision, the reaction stability and the equipment operation efficiency are improved, and the device is suitable for high-density and high-flux DNA synthesis scenes.

The present application relates to a DNA encoding method, decoding method, device, electronic device and medium. The method includes: obtaining binary data to be encoded; grouping the binary data to obtain a plurality of binary data groups; for each binary data group, when the first binary bit of the binary data group is 0, obtaining a preset first mapping table; encoding the binary data group into corresponding base units according to the preset first mapping table; when the first binary bit of the binary data group is 1, obtaining a preset second mapping table; encoding the binary data group into corresponding base units according to the preset second mapping table; merging the corresponding base units of each binary data group to obtain a base sequence corresponding to the binary data, thereby improving the accuracy of DNA synthesis and sequencing.

Provided herein are compositions, devices, systems and methods for constructing and storing polynucleotides encoding information with redox resistant bases. The compositions, devices, systems, and methods described herein provide for storage or synthesis of a library comprising a plurality of polynucleotides with one or more redox resistance bases. Further provided herein are methods to increase DNA synthesis yield and fidelity.

This invention belongs to the fields of molecular biology and bioinformatics, specifically relating to a truncated bovine virus-encoded protein phage library, its construction method, and applications. This library covers all 162 known bovine viruses that host bovine subfamily viruses, containing 51,794 non-redundant peptide coding sequences of 56 amino acids each. These peptides are fused and displayed on the surface of the T7 phage capsid, with all coding sequences having a uniform total length of 200 bp. The library construction was achieved through sequence acquisition, truncation and redundancy removal, codon optimization and DNA synthesis, and in vitro ligation and packaging with T7 phage. The resulting library exhibits a positivity rate of 93% and a titer of 2.8 × 10⁻⁶. 10 PFU / mL. This invention features an optimized single-round high-throughput screening process adapted to clinical bovine serum samples, enabling efficient identification of bovine virus B-cell epitopes and providing tools and candidate targets for bovine virus vaccine development and diagnostic reagent development.

The invention relates to a high-flux enzymatic DNA synthesis method based on framework nucleic acid. Specifically, the invention provides a TdT-framework nucleic acid compound which can accurately regulate and control the position and distance of terminal deoxynucleotidyl transferase (TdT) so as to achieve the purpose of high-flux enzymatic DNA synthesis. The invention also provides a method for preparing the TdT-framework nucleic acid compound, and a method for synthesizing DNA (Deoxyribonucleic Acid) by using the TdT-framework nucleic acid compound. When the TdT-framework nucleic acid compound is used for synthesizing DNA, high-flux enzymatic DNA synthesis can be realized, and the TdT-framework nucleic acid compound has important significance for developing a high-flux DNA synthesis technology with mild conditions.

The invention relates to a DNA synthesis chip, a DNA synthesis method and application thereof, the DNA synthesis chip comprises: a first substrate, the first substrate is provided with a plurality of first grooves and a plurality of electrode groups, which are distributed at intervals; the electrode group comprises a first electrode and a second electrode; each first groove is in contact with one first electrode and one second electrode; the second substrate is provided with a reagent injection channel; the first substrate and the second substrate are configured to be combined with each other; when the first substrate and the second substrate are combined, the reagent injection channel is communicated with the first grooves, and a runner communicated with each first groove is arranged between the first substrate and the second substrate; the first substrate is provided with a first surface facing the second substrate. According to the DNA synthesis method disclosed by the embodiment of the invention, the difficulty of chip modification is reduced, the possibility of crosstalk is reduced, the synthesis amount is increased, and the chip can be repeatedly used, so that the synthesis cost is greatly reduced.

The invention relates to the technical field of DNA information storage, and discloses a method for prolonging data storage time and application, the method comprises the following steps: data is converted into a DNA sequence with a preset length, DNA is synthesized and stored, primary amino groups in basic groups of the DNA are independently modified by protective groups, the protective groups are selected from-COR, and the primary amino groups in the basic groups of the DNA are independently modified by the protective groups. R is selected from alkyl of C1-C6, substituted or unsubstituted naphthenic base of C3-C8 or substituted or unsubstituted phenyl. According to the method for prolonging the data storage time, the degradation rate of DNA can be remarkably inhibited, the stability of the DNA can be improved so as to realize long-time stable storage of the data, and the method is particularly suitable for forming a DNA storage scene with limited conditions, namely, the method is suitable for DNA synthesis in large companies with advanced technologies and equipment and also suitable for large companies with advanced technologies and equipment. The method is also suitable for DNA synthesis of small companies, and has application prospects and potential.

The invention relates to application of an active component in preparation of a medicine for treating tumor diseases. Specifically, it is found for the first time that small-molecule isovaleric acid and the prodrug thereof can inhibit DNA homologous recombination so as to efficiently inhibit tumor growth, and isovaleric acid and the prodrug thereof can be combined with chemotherapeutic drugs for radiotherapy, DNA damage induction or DNA synthesis inhibition to generate a synergistic tumor treatment effect.

The present invention relates to an in vitro cell-free process for production of deoxyribonucleotides (DNAs) comprising at least one hairpin, corresponding DNA products and uses thereof, and oligonucleotides and kits useful in the process of the invention.

The invention relates to a T7RNA polymerase mutant for synthesizing single-stranded DNA as well as a screening method and application of the T7RNA polymerase mutant, and belongs to the technical field of enzyme engineering. The invention provides a method for directed evolution and screening of a T7RNA polymerase mutant, which comprises the following steps: knocking out exonuclease and mismatch repair protein from a chassis strain genome, knocking in single-stranded annealing protein, resistance gene containing deletion mutation and a single-stranded DNA synthesis template, and constructing to obtain a genetically engineered bacterium for screening; a T7RNA polymerase mutant library is constructed, recombinant plasmids with the mutant library are transferred into engineering bacteria, T7RNA polymerase mutants synthesize single-stranded DNA to repair deletion mutation of resistance genes, the transformed engineering bacteria can grow on a resistance plate, the T7RNA polymerase mutants obtained through multiple rounds of screening can efficiently synthesize the single-stranded DNA through in-vitro verification, and the T7RNA polymerase mutant can be used for preparing the single-stranded DNA. A brand new tool is provided for synthesis of single-stranded DNA, and the method has the potential of being applied to the fields of gene editing, evolutionary engineering and the like.

This invention relates to a dynamic scanning compensation method and DNA synthesis device based on inkjet printing, belonging to the field of DNA synthesis. By applying threshold segmentation, connected component analysis, and circular Hough transform to a standard analysis image, a circular region for each reaction site is obtained. Based on the distance of all edge points of each reaction site from the center of the circular boundary, it is determined whether each reaction site meets the roundness requirements, thus obtaining information on missing reaction sites. Simultaneously, to address potential inaccuracies after the circular Hough transform, the results are calibrated using the YOLO target detection model based on a convolutional neural network, achieving high-precision detection of missing reaction sites suitable for industrial applications. Furthermore, by adding recognition weights for defective reaction sites such as those with "partially excessive distances from the center" during YOLOv6 model training, the compatibility between the two is further improved, enhancing detection accuracy and meeting the requirements of high-precision DNA synthesis.

The invention provides a construction method for a tissue paraffin section space proteomics sequencing library. The method comprises the following steps: providing a solid phase carrier and an antibody-nucleic acid conjugate; attaching a tissue paraffin section sample to the solid-phase carrier, and carrying out section baking, dewaxing, hydration, decrosslinking and sealing treatment to obtain a sealed solid-phase carrier; incubating the antibody-nucleic acid conjugate and the closed solid-phase carrier to obtain a protein capture solid-phase carrier; carrying out permeabilization treatment and DNA synthesis on the protein capture solid phase carrier to obtain cDNA; releasing the cDNA from the solid-phase carrier, collecting the cDNA, and amplifying to obtain the sequencing library.

The application discloses an iron ion chelator based on adenine and synthesis and application thereof, and synthesizes a series of iron ion chelators with high affinity to ribonucleotide reductase, taking adenine as a leading compound, and the iron ion chelators have tridentate coordination donors (OH, N, N or N, N, N). In a salvage synthesis pathway, when the adenine derivatives as DNA synthesis raw materials contact with RR, the iron ions in the active center of the RR are effectively chelated, and then the activity of the RR is inhibited, the synthesis of DNA is blocked, and finally the proliferation of tumor cells is inhibited. The application reasonably utilizes the synthesis of DNA of cancer cells through a free base salvage synthesis pathway, and finds a new breakthrough for the iron ion chelator used for tumor treatment.

The application provides a nucleoside dimer, a preparation method thereof and application in DNA synthesis. The nucleoside dimer of the application adopts a mixed skeleton of methyl phosphate and beta-cyanoethyl phosphoramidite, a new preparation method of the nucleoside dimer is obtained by optimizing a synthesis method and a purification process, and a foundation for high-quality DNA synthesis is laid. On this basis, the nucleoside dimer with the new skeleton is applied to DNA synthesis, an oligonucleotide fragment synthesis method suitable for the dimer is established, and an oligonucleotide fragment with a length of 100 nt is synthesized. Fidelity experiment shows that the error rate of the DNA fragment synthesized by using the dimer is only 3.75 errors / Kb, and high-fidelity DNA synthesis is realized. The preparation method of the nucleoside dimer with the new skeleton can realize large-scale and high-purity preparation, the nucleoside dimer can play an important role in improving the length and fidelity of oligonucleotide synthesis, and has practical value.

A DNA synthesis method is provided. The DNA synthesis method according to some embodiments is a method for synthesizing a target DNA to be used as a data storage medium, and may comprise the steps of: preparing a template DNA; and synthesizing the target DNA from the template DNA on the basis of a DNA polymerase. Here, the sequence of the template DNA includes a universal base sequence, which can complementarily bind to any one of two or more types of data DNA bases, and a stop base sequence, wherein a starting base of the stop base sequence does not complementarily bind to the data DNA bases and can complementarily bind to a pre-designated non-data DNA base. According to this method, a target DNA having a desired sequence can be accurately and quickly synthesized in an eco-friendly manner.

Described herein are compositions and methods for polynucleotide synthesis. The composition for synthesis can comprise a nucleoside; and at least one protecting group, wherein the protecting group is configured for electrochemical removal under mild conditions. Methods include extending a polynucleotide, wherein the polynucleotide is extended by deprotecting a first protected nucleotide to generate a deprotected nucleotide. Methods may further include contacting the deprotected nucleotide with a second protected nucleotide thereby extending the polynucleotide.

The invention discloses an electrochemical DNA synthesis and control method based on an updatable metal substrate, which comprises the following steps: preparing a liquid metal microelectrode or an electrode array, and carrying out electrochemical DNA synthesis on the surface of the electrode; after the phase change of the obtained electrode, the endocytosis of the DNA molecules on the surface of the electrode is realized by manually or automatically mechanically extruding the liquid metal, so that electrochemical synthesis and in-situ packaging are completed; the liquid metal oxide layer is removed to release internal molecules; after the liquid metal is extruded out, new liquid metal flows to the original position, or after the liquid metal is taken out of the array, new liquid metal is added in the original position of the array, and electrode or electrode array updating is achieved and used for a new round of synthetic reaction and molecular manipulation. The method provided by the invention is simple and efficient, can realize updating of the electrode for DNA synthesis and integrated molecular control, has extremely high expandability, and provides a new thought for further development of a DNA synthesis platform and practical application in the field of DNA data storage.

According to the super-hydrophobic surface for DNA synthesis and the preparation method of the super-hydrophobic surface, a patterned hydrophobic microstructure and a super-hydrophobic coating are organically combined on the surface, and the surface hydrophobicity is remarkably enhanced. The super-hydrophobic coating is constructed by adopting a two-step method and comprises a hydrophobic resin coating and a silane hydrophobic coating, durability and hydrophobicity are both considered, the hydrophobic coating is not prepared by adopting a common fluorocarbon chain, and a synthetic surface which is efficient, durable, simple in structure and good in biological safety is provided for DNA synthesis.

The invention discloses a DNA encoding method and device and a DNA decoding method and device, and belongs to the technical field of information storage and security. The DNA encoding method comprises the steps that target information is converted into computer codes; converting the computer code into a DNA coding chain or a DNA coding chain combination; and cross-linking the DNA coding chain and / or the DNA coding chain combination in a storage micro-pool on a DNA information storage medium so as to realize the storage of the target information. According to the method, the information storage process is completely independent of DNA synthesis and DNA sequencing technologies, and the method has the advantages of high parallelism, short access time, good encryption, low cost, strong expansibility and the like.

The invention discloses an automatic cutting system and method for a multi-chain DNA synthetic rubber plate. The system comprises an MES control system, a digital twin system, a rubber plate pretreatment system, a rubber plate cutting system, a colored tape detection camera, a colored tape pushing system, a colloidal particle extrusion system and a colloidal particle collection device. Through the MES control system, the digital twin system, the rubber plate pretreatment system, the rubber plate cutting system, the colored tape detection camera, the colored tape pushing system, the rubber particle extrusion system and the rubber particle collection device, automatic cutting and collection of rubber plates synthesized by multi-chain DNA are realized; the requirements of high-throughput experiments are met, complex strips are accurately distinguished, and the situation that cutting position deviation affects the uniformity of colloidal particles and the subsequent purification efficiency is avoided; the single-time positioning error of a mechanical arm base coordinate system is small, and the requirement for millimeter-level DNA colloidal particle cutting precision can be met; and multi-station continuous processing is realized, and the processing efficiency, precision and experiment consistency are improved.

The present invention relates to a DNA synthesis device, comprising: a liquid reservoir group, the liquid reservoir group including a plurality of liquid storage bottles; a syringe pump group, the syringe pump group including a plurality of syringe pumps, the input ends of the syringe pumps being connected to the corresponding liquid storage bottles via pipelines; a multi-channel switching valve, the output ends of the plurality of syringe pumps being connected to the input port via pipelines; a microfluidic chip, the input end of the microfluidic chip being connected to the output port of the multi-channel switching valve via pipelines; a waste liquid bottle, the waste liquid bottle being connected to the output end of the microfluidic chip via pipelines; and a PLC controller, the PLC controller being electrically connected to the syringe pump group and used to control the operation of each syringe pump and the multi-channel switching valve. The DNA synthesis device optimizes the internal circuit and control system of the device, utilizes syringe pumps to accurately control the amount of reagents and consumables, saves resources, and avoids cross contamination between liquids.

The invention relates to the technical field of DNA data storage, in particular to a DNA information encryption and error correction method based on secondary structure fingerprint separation storage, which comprises an information DNA encoding stage, a structure signature generation stage, a signature DNA encoding stage, an information DNA and signature DNA synthesis stage and a decoding and error correction stage. The information DNA encoding stage is used for encoding original data into information DNA fragments; the structure signature generation stage is used for calculating structure characteristic parameters, generating a compact structure signature, and associating the fragment ID with the compact structure signature to form a signature data set; the signature DNA encoding stage is used for encoding the signature data set into signature DNA fragments; the information DNA and signature DNA synthesis stage is used for respectively synthesizing an information DNA fragment and a signature DNA fragment, and physically separating and storing the information DNA fragment and the signature DNA fragment; and the decoding and error correction stage is used for verifying and correcting the consensus sequence based on the signature data set to recover the original data. According to the method, the structure signature is stored in the independent DNA molecule, so that physical layer encryption protection is realized.

The application discloses a kind of ancient text sequence conversion method and system for DNA storage data based on five-stroke encoding, comprising the following steps: ancient text is converted into five-stroke encoding alphabet sequence according to word;Five-stroke encoding alphabet sequence is compressed by Huffman ternary, and ternary digital string is obtained;Ternary digital string is converted into DNA base sequence by dynamic mapping rule;DNA base sequence is segmented, and positioning code is added to each segment, and the final DNA sequence set suitable for DNA synthesis and high-throughput sequencing is output;The final DNA sequence set is synthesized, sequenced, and the ancient text is restored by reverse operation, and its reliability is verified.The method can efficiently and accurately convert ancient text to DNA storage sequence, ensure biological compatibility and data integrity restoration, and meet the long-term DNA storage needs of ancient text.

This invention discloses an siRNA for inhibiting MCM7, a composition thereof, and its application. The siRNA designed and verified by this invention can efficiently inhibit MCM7 gene expression, thereby inhibiting DNA synthesis, cell proliferation, and cell clone generation in cancer cells, achieving the purpose of preventing and treating tumors. This invention provides a new target and candidate compound for cancer prevention or treatment.

The invention provides a jet state detection method of a DNA ink-jet printer, which comprises the following steps: a target detection step: detecting a jet orifice and a test tube through an RT-DETR model to obtain a corresponding jet orifice detection result and a test tube detection result; and a target matching step: matching the jet orifice detection result with the test tube detection result through a predetermined matching algorithm. The invention further provides an injection state detection device of the DNA ink-jet printer, a storage medium and electronic equipment. Therefore, the detection precision and efficiency can be improved in an automatic and intelligent manner, so that the stability and reliability in the DNA synthesis process are ensured.

The application discloses functions and application of KIF5B gene in regulating physiological activity of myoblasts, and belongs to the technical field of animal cell culture. By investigating influences of KIF5B knockdown and overexpression on proliferation of myoblasts, it is found that KIF5B knockdown can significantly inhibit DNA synthesis speed of myoblasts, thereby inhibiting proliferation. In addition, overexpression of KIF5B can significantly inhibit expression of MYOD and MYOG, improve DNA synthesis speed of myoblasts, thereby significantly improving proliferation rate of myoblasts. The application provides an important key molecular target and technical support for efficient and rapid culture of myogenic cells.

The invention discloses a synthetic method of a mixed DNA chain containing a non-natural basic group, a non-natural basic group recognition and detection method and application of the non-natural basic group recognition and detection method. According to the method, the mixture of the non-natural base and the natural base nucleotide is added in the DNA synthesis process, special mixed DNA synthesis is achieved, and non-natural base recognition and information reduction are achieved by means of error recognition of specific sites. Compared with an existing information storage method, the method has the advantages of being large in storage density, compatible with traditional synthesis and sequencing methods and the like; and the non-natural basic group can be quickly and accurately identified by utilizing the error identification of the sequencing result.