56 results about "DNA synthesis" patented technology

This invention belongs to the fields of molecular biology and bioinformatics, specifically relating to a truncated bovine virus-encoded protein phage library, its construction method, and applications. This library covers all 162 known bovine viruses that host bovine subfamily viruses, containing 51,794 non-redundant peptide coding sequences of 56 amino acids each. These peptides are fused and displayed on the surface of the T7 phage capsid, with all coding sequences having a uniform total length of 200 bp. The library construction was achieved through sequence acquisition, truncation and redundancy removal, codon optimization and DNA synthesis, and in vitro ligation and packaging with T7 phage. The resulting library exhibits a positivity rate of 93% and a titer of 2.8 × 10⁻⁶. 10 PFU / mL. This invention features an optimized single-round high-throughput screening process adapted to clinical bovine serum samples, enabling efficient identification of bovine virus B-cell epitopes and providing tools and candidate targets for bovine virus vaccine development and diagnostic reagent development.

The invention relates to a DNA synthesis chip, a DNA synthesis method and application thereof, the DNA synthesis chip comprises: a first substrate, the first substrate is provided with a plurality of first grooves and a plurality of electrode groups, which are distributed at intervals; the electrode group comprises a first electrode and a second electrode; each first groove is in contact with one first electrode and one second electrode; the second substrate is provided with a reagent injection channel; the first substrate and the second substrate are configured to be combined with each other; when the first substrate and the second substrate are combined, the reagent injection channel is communicated with the first grooves, and a runner communicated with each first groove is arranged between the first substrate and the second substrate; the first substrate is provided with a first surface facing the second substrate. According to the DNA synthesis method disclosed by the embodiment of the invention, the difficulty of chip modification is reduced, the possibility of crosstalk is reduced, the synthesis amount is increased, and the chip can be repeatedly used, so that the synthesis cost is greatly reduced.

The invention relates to the technical field of DNA information storage, and discloses a method for prolonging data storage time and application, the method comprises the following steps: data is converted into a DNA sequence with a preset length, DNA is synthesized and stored, primary amino groups in basic groups of the DNA are independently modified by protective groups, the protective groups are selected from-COR, and the primary amino groups in the basic groups of the DNA are independently modified by the protective groups. R is selected from alkyl of C1-C6, substituted or unsubstituted naphthenic base of C3-C8 or substituted or unsubstituted phenyl. According to the method for prolonging the data storage time, the degradation rate of DNA can be remarkably inhibited, the stability of the DNA can be improved so as to realize long-time stable storage of the data, and the method is particularly suitable for forming a DNA storage scene with limited conditions, namely, the method is suitable for DNA synthesis in large companies with advanced technologies and equipment and also suitable for large companies with advanced technologies and equipment. The method is also suitable for DNA synthesis of small companies, and has application prospects and potential.

This invention relates to a dynamic scanning compensation method and DNA synthesis device based on inkjet printing, belonging to the field of DNA synthesis. By applying threshold segmentation, connected component analysis, and circular Hough transform to a standard analysis image, a circular region for each reaction site is obtained. Based on the distance of all edge points of each reaction site from the center of the circular boundary, it is determined whether each reaction site meets the roundness requirements, thus obtaining information on missing reaction sites. Simultaneously, to address potential inaccuracies after the circular Hough transform, the results are calibrated using the YOLO target detection model based on a convolutional neural network, achieving high-precision detection of missing reaction sites suitable for industrial applications. Furthermore, by adding recognition weights for defective reaction sites such as those with "partially excessive distances from the center" during YOLOv6 model training, the compatibility between the two is further improved, enhancing detection accuracy and meeting the requirements of high-precision DNA synthesis.

The invention provides a construction method for a tissue paraffin section space proteomics sequencing library. The method comprises the following steps: providing a solid phase carrier and an antibody-nucleic acid conjugate; attaching a tissue paraffin section sample to the solid-phase carrier, and carrying out section baking, dewaxing, hydration, decrosslinking and sealing treatment to obtain a sealed solid-phase carrier; incubating the antibody-nucleic acid conjugate and the closed solid-phase carrier to obtain a protein capture solid-phase carrier; carrying out permeabilization treatment and DNA synthesis on the protein capture solid phase carrier to obtain cDNA; releasing the cDNA from the solid-phase carrier, collecting the cDNA, and amplifying to obtain the sequencing library.

A DNA synthesis method is provided. The DNA synthesis method according to some embodiments is a method for synthesizing a target DNA to be used as a data storage medium, and may comprise the steps of: preparing a template DNA; and synthesizing the target DNA from the template DNA on the basis of a DNA polymerase. Here, the sequence of the template DNA includes a universal base sequence, which can complementarily bind to any one of two or more types of data DNA bases, and a stop base sequence, wherein a starting base of the stop base sequence does not complementarily bind to the data DNA bases and can complementarily bind to a pre-designated non-data DNA base. According to this method, a target DNA having a desired sequence can be accurately and quickly synthesized in an eco-friendly manner.

Described herein are compositions and methods for polynucleotide synthesis. The composition for synthesis can comprise a nucleoside; and at least one protecting group, wherein the protecting group is configured for electrochemical removal under mild conditions. Methods include extending a polynucleotide, wherein the polynucleotide is extended by deprotecting a first protected nucleotide to generate a deprotected nucleotide. Methods may further include contacting the deprotected nucleotide with a second protected nucleotide thereby extending the polynucleotide.

According to the super-hydrophobic surface for DNA synthesis and the preparation method of the super-hydrophobic surface, a patterned hydrophobic microstructure and a super-hydrophobic coating are organically combined on the surface, and the surface hydrophobicity is remarkably enhanced. The super-hydrophobic coating is constructed by adopting a two-step method and comprises a hydrophobic resin coating and a silane hydrophobic coating, durability and hydrophobicity are both considered, the hydrophobic coating is not prepared by adopting a common fluorocarbon chain, and a synthetic surface which is efficient, durable, simple in structure and good in biological safety is provided for DNA synthesis.

The invention discloses a DNA encoding method and device and a DNA decoding method and device, and belongs to the technical field of information storage and security. The DNA encoding method comprises the steps that target information is converted into computer codes; converting the computer code into a DNA coding chain or a DNA coding chain combination; and cross-linking the DNA coding chain and / or the DNA coding chain combination in a storage micro-pool on a DNA information storage medium so as to realize the storage of the target information. According to the method, the information storage process is completely independent of DNA synthesis and DNA sequencing technologies, and the method has the advantages of high parallelism, short access time, good encryption, low cost, strong expansibility and the like.

The invention relates to the technical field of DNA data storage, in particular to a DNA information encryption and error correction method based on secondary structure fingerprint separation storage, which comprises an information DNA encoding stage, a structure signature generation stage, a signature DNA encoding stage, an information DNA and signature DNA synthesis stage and a decoding and error correction stage. The information DNA encoding stage is used for encoding original data into information DNA fragments; the structure signature generation stage is used for calculating structure characteristic parameters, generating a compact structure signature, and associating the fragment ID with the compact structure signature to form a signature data set; the signature DNA encoding stage is used for encoding the signature data set into signature DNA fragments; the information DNA and signature DNA synthesis stage is used for respectively synthesizing an information DNA fragment and a signature DNA fragment, and physically separating and storing the information DNA fragment and the signature DNA fragment; and the decoding and error correction stage is used for verifying and correcting the consensus sequence based on the signature data set to recover the original data. According to the method, the structure signature is stored in the independent DNA molecule, so that physical layer encryption protection is realized.

The application discloses a kind of ancient text sequence conversion method and system for DNA storage data based on five-stroke encoding, comprising the following steps: ancient text is converted into five-stroke encoding alphabet sequence according to word;Five-stroke encoding alphabet sequence is compressed by Huffman ternary, and ternary digital string is obtained;Ternary digital string is converted into DNA base sequence by dynamic mapping rule;DNA base sequence is segmented, and positioning code is added to each segment, and the final DNA sequence set suitable for DNA synthesis and high-throughput sequencing is output;The final DNA sequence set is synthesized, sequenced, and the ancient text is restored by reverse operation, and its reliability is verified.The method can efficiently and accurately convert ancient text to DNA storage sequence, ensure biological compatibility and data integrity restoration, and meet the long-term DNA storage needs of ancient text.

This invention discloses an siRNA for inhibiting MCM7, a composition thereof, and its application. The siRNA designed and verified by this invention can efficiently inhibit MCM7 gene expression, thereby inhibiting DNA synthesis, cell proliferation, and cell clone generation in cancer cells, achieving the purpose of preventing and treating tumors. This invention provides a new target and candidate compound for cancer prevention or treatment.

The application discloses functions and application of KIF5B gene in regulating physiological activity of myoblasts, and belongs to the technical field of animal cell culture. By investigating influences of KIF5B knockdown and overexpression on proliferation of myoblasts, it is found that KIF5B knockdown can significantly inhibit DNA synthesis speed of myoblasts, thereby inhibiting proliferation. In addition, overexpression of KIF5B can significantly inhibit expression of MYOD and MYOG, improve DNA synthesis speed of myoblasts, thereby significantly improving proliferation rate of myoblasts. The application provides an important key molecular target and technical support for efficient and rapid culture of myogenic cells.

The invention discloses a composition containing a G4 stabilizer and a TLS pathway inhibitor and application of the composition. The G4 stabilizer is TMPyP4, the TLS pathway inhibitor is JH-RE-06, and the mass ratio of the G4 stabilizer to the TLS pathway inhibitor is (15-30): 1; the composition can be used for preparing drugs for preventing or treating pseudorabies virus infection or diseases related to pseudorabies virus infection, or can be used for preparing products for inhibiting replication and proliferation of pseudorabies virus or DNA synthesis. Therefore, the invention provides a novel antiviral strategy of blocking virus DNA synthesis through double targets instead of directly acting on virus protein, and provides a new drug research and development path and treatment thought for coping with PRV variants and breaking through the insufficient protection of existing vaccines.

This invention discloses an siRNA for inhibiting MCM7, a composition thereof, and its application. The siRNA designed and validated in this invention can efficiently inhibit MCM7 gene expression, thereby inhibiting DNA synthesis, cell proliferation, and cell clone generation in cancer cells, achieving the purpose of preventing and treating tumors. This invention provides a new target and candidate drug for cancer prevention or treatment.

The application provides a high-loading nucleic acid synthesis chip and a preparation method thereof. The chip comprises a chip substrate, a micropore array and microspheres which are layered on the inner wall of the micropores. The micropore array is a through hole which penetrates the chip substrate from top to bottom, and the inner wall of the through hole is self-assembled and fixed with the microspheres. The microspheres are uniformly and orderly arranged in a layered structure. The chip is prepared through five steps of cleaning the chip and a container, preparing a microsphere colloidal solution, self-assembling the microspheres by using a vertical deposition method, fixing the self-assembled structure by using a 'cold sintering' method and obtaining an expected structure by multiple self-assembling of the microspheres and fixing. By increasing the effective synthesis area of the reaction pool of the DNA synthesis chip, the DNA synthesis loading is improved, the high-loading chip is prepared, and the problem of low loading of the DNA chip is solved.

The invention discloses a stable and high-efficiency high-fidelity DNA polymerase mutant and a preparation method thereof, relates to the fields of genetic engineering and minute biology, and aims to modify a high-fidelity DNA polymerase by rationally designing mutation sites and optimizing additives, enhance the thermal stability, extension efficiency and continuous synthesis capability of the high-fidelity DNA polymerase and maintain the high-fidelity characteristic of the high-fidelity DNA polymerase. The optimized DNA polymerase shows significantly improved amplification speed in the PCR amplification process, can efficiently synthesize longer DNA fragments, and is suitable for amplification of complex templates (such as sequences with high GC content or rich secondary structures). According to the method, the synthesis efficiency and accuracy of the long-fragment DNA are remarkably improved, and a more efficient tool is provided for genome sequencing, gene cloning, in-vitro diagnosis and the like.

The invention discloses a liquid path supply system and method for DNA synthesis and a DNA synthesis system. Wherein the liquid path supply system comprises a reaction reagent supply system, a switching system and a cleaning source supply system, and the cleaning source supply system comprises a reaction reagent cleaning system matched with the reaction reagent supply system and a valve terminal cleaning system matched with the switching system; the switching system comprises a multiple one-in-one valve terminal and a reagent switching assembly, an outlet and a plurality of inlets are formed in the side face of the valve terminal, an input branch channel, a conveying branch channel and an output main channel are arranged in the valve terminal, one end of the input branch channel communicates with the corresponding inlet, and the other end of the input branch channel is connected with the switching system; the conveying branch channel is connected with an outlet of the switching system; outlets of all the conveying branch channels converge in the same intersection area, the intersection area communicates with an inlet of the output main channel, and an outlet of the output main channel communicates with the public outlet. According to the method, different reagents can be switched into the public pipeline corresponding to the closed reaction cavity in a pollution-free manner.

The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcribing DNA into RNA and an automated device for RNA production. In addition, the use of a bioreactor for RNA in vitro transcription as described herein is part of the invention. The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP compliance conditions. In particular, the RNA in vitro transcription reactor allows a DNA template to be repeatedly used for various RNA in vitro transcription reactions. Furthermore, the present invention relates to a device for RNA production comprising: (a) a module for template DNA synthesis, (b) a module for transcribing DNA to RNA, the module comprising the RNA in vitro transcription reactor; and optionally (c) a module for an RNA preparation.

The present application relates to the field of biological medicine, and particularly relates to a kind of nanocomposite and its preparation method and application in postoperative treatment of pancreatic cancer.The nanocomposite is that RRM2 siRNA is compounded with gold nanocluster AuNCs, then is loaded into mesenchymal stem cell-derived exosome MSC-EVs, and the nanocomposite AuNC-siRNA / EV is prepared.The present application can generate nanocomposite with high drug loading efficiency and lysosome escape ability by electrostatic self-assembly of gold nanoparticles and siRNA and membrane fusion technology of MSC-EVs.RRM2 siRNA can be accurately delivered to residual tumor lesions using the natural homing characteristics of MSC-EVs, thereby inhibiting RRM2 expression, blocking DNA synthesis and stem cell characteristics of tumor cells, and further inhibiting micro-metastasis proliferation and immune escape, which opens up a new paradigm for postoperative treatment of pancreatic cancer.

1. Name of the product in this design: DNA Synthesizer. 2. Purpose of this design: for DNA synthesis. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention discloses an efficient enzymatic DNA synthesis method, and belongs to the technical field of DNA synthesis. The technical problem to be solved by the invention is how to reduce the probability of formation of a secondary structure in the enzymatic DNA synthesis process or damage the formation of the secondary structure and improve the accuracy and yield of enzymatic DNA synthesis. In order to solve the technical problem, the invention provides the efficient enzymatic DNA synthesis method, the method can comprise the steps of providing a composition for enzymatic DNA synthesis reaction, enabling the composition to react to obtain DNA, and the composition comprises the following components: a starting chain, TdT enzyme, 7-deaza-dGTP modified with a reversible blocking group at the 3'end and nucleotide modified with a reversible blocking group at the 3 'end; and the nucleotides are dATP, dCTP, dGTP and dTTP.

The present invention provides a method for non-template dependent synthesis of oligo- and polynucleotides, which comprises immobilization of an Abi enzyme to facilitate the formation of a growing ssDNA by subsequent contacting with nucleotide triphosphates and final release of the oligo- or polynucleotide.

The embodiment of the present application provides a solid phase carrier for enzymatic DNA synthesis and a preparation method and application thereof. The solid phase carrier comprises a hydrogel skeleton material, a functional material, a crosslinking agent, an additive and an initiation chain; the hydrogel skeleton material comprises an acrylate polymer material; the additive comprises a reinforcing agent, a plasticizer and a photoinitiator; wherein the hydrogel skeleton material accounts for 10-35% of the total mass of each component. Compared with the prior art, the present application has at least one of the following beneficial effects: strong stretching ability, low steric hindrance and high synthesis efficiency.

The present invention relates to an improved method for the synthesis of deoxyribonucleic acids (DNAs), in particular the cell-free enzymatic synthesis of DNA, preferably on a large scale, with increased yield and / or increased efficiency. Cationic species present as counterions in nucleotide salts are crucial for yield, efficiency, and accuracy of high yield enzymatic DNA synthesis reactions. The methods herein use alternative cations as counterions for ionic nucleotides, allowing for the use of higher concentrations of nucleotides in DNA synthesis, and further allowing for the use of more advantageous reaction conditions.

The application belongs to the technical field of biology, and specifically provides a UCRT heat-resistant reverse transcriptase mutant with improved thermal stability, the mutant including four single-point mutants, six double mutants and four triple mutants, compared with the wild-type UCRT III heat-resistant reverse transcriptase, the mutant has a longer half-life at 65 DEG C; the double mutant has a better effect, and the half-life thereof is about 4 times that of the wild-type DNA polymerase. The application further provides a genetically engineered bacterium containing the mutant. The UCRT heat-resistant reverse transcriptase mutant obtained through the construction method has better thermal stability, and when reverse transcription and DNA synthesis are performed at a higher temperature, the mutant has higher thermal stability, and has great application potential.

The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of low levels of nucleotides and at a high temperature, such as temperatures at or near the melting temperature of the polymerase.

The present application relates to a kind of multifunctional layered double hydroxide (LDH) based therapeutic nano vaccine and preparation method and application.The nano vaccine is loaded with immunoadjuvant CpG and chemotherapeutic drug 5-fluorouracil (5-FU) in turn with LDH as carrier, and further coated with apoptotic tumor cell membrane (aCM), to construct a nano vaccine with immune synergistic therapy function.After being injected into mouse body through tail vein, the LDH-based therapeutic nano vaccine realizes multi-mode combined therapy for tumor through the endoplasmic reticulum stress amplification effect mediated by calcium ion overload, through 5-FU to inhibit DNA synthesis and interfere RNA function in tumor cells, and through the synergistic immune activation mechanism mediated by the double adjuvant system composed of aluminum ion and CpG.The strategy not only significantly inhibits the growth of in situ tumor, but also can induce the body to produce immune memory, and plays an effective tumor prevention effect.

The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcription of DNA into RNA and an automated device for RNA manufacturing. Furthermore, the use of a bioreactor for RNA in vitro transcription as described herein is part of the present invention. The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP compliant conditions. In particular, the RNA in vitro transcription reactor allows for repeated use of a DNA template for various RNA in vitro transcription reactions. Furthermore, the present invention relates to a device for RNA manufacturing comprising (a) a module for template DNA synthesis, (b) a module for transcription of DNA into RNA, the module comprising the RNA in vitro transcription reactor; and optionally (c) a module for RNA formulation.