129 results about "DNA synthesis" patented technology

The invention discloses a semantic intelligent enhanced DNA storage method for the Internet of Things, and belongs to the crossing field of artificial intelligence and biological information storage, the compression efficiency and robustness of DNA image storage are effectively improved through introduced semantic extraction and a multi-read screening mechanism of design, key information areas in images are extracted through semantic extraction, and the compression efficiency and robustness of DNA image storage are improved. The method has the advantages that redundant backgrounds are eliminated, coding lengths are reduced, high-quality sequences are selected from sequencing copies by the aid of scoring and sequence analysis through a multi-read screening mechanism, fault tolerance is enhanced, the method is applicable to efficient semantic storage in scenes of the internet of things, the problem of low storage efficiency due to excessive redundant information in original images in existing DNA image storage methods is solved, and the method is applicable to high-efficiency semantic storage in scenes of the internet of things. And a high bit error rate exists in the DNA synthesis and sequencing process, so that the image recovery quality is influenced.

The invention discloses a DNA synthesis method which comprises the following steps: processing an initial substrate according to a preset strategy to obtain a target substrate with a Mark pattern and a plurality of reaction sites, and adjusting the positions of the target substrate and an ink-jet printing device through the Mark pattern, so that the working directions of a reaction unit array and the device are consistent, and the subsequent operation accuracy is ensured; deprotecting the reaction sites to generate active groups; sequentially spraying a target monomer solution and an activator solution into the reaction sites of the preset area after deprotection treatment through an ink-jet printing device, and carrying out a coupling reaction with the active groups to obtain a mixed solution containing a nucleotide chain; carrying out cleaning, oxidation, capping and deprotection treatment on the mixed solution to obtain a single-layer base product; according to the target base sequence, determining a corresponding target monomer solution, and circularly carrying out a reaction to obtain a mixed solution containing a nucleotide chain; and obtaining the synthesized DNA according to the mixed solution. According to the method, high-throughput, high-quality and high-efficiency DNA synthesis can be realized.

The invention relates to a high-throughput DNA synthesis device based on ink-jet printing. The high-throughput DNA synthesis device comprises a plurality of ink boxes, a sealing assembly, a lifting assembly, a bearing jig and a moving assembly. Each ink box is arranged at the corresponding ink jet station and is used for temporarily storing the DNA basic group reagent and jetting the DNA basic group reagent through an ink jet port, and the ink jet port can automatically control the volume of liquid drops. The bearing jig is used for bearing the reaction pore plate. And the plurality of sealing assemblies are respectively arranged at the reaction stations and are used for being in sealing fit with the bearing jig to form a closed reaction cavity. The multiple lifting assemblies are arranged on the connection stations below the reaction station and used for driving the bearing jig to rise, and the reaction pore plate is matched with the sealing assembly. The moving assembly is used for driving the bearing jig to move between the ink jet station and the connection station. According to the invention, the parallel operation of reagent ink jet, closed reaction and reaction and sample adding is realized, the liquid drop control precision, the reaction stability and the equipment operation efficiency are improved, and the device is suitable for high-density and high-flux DNA synthesis scenes.

The present application relates to a DNA encoding method, decoding method, device, electronic device and medium. The method includes: obtaining binary data to be encoded; grouping the binary data to obtain a plurality of binary data groups; for each binary data group, when the first binary bit of the binary data group is 0, obtaining a preset first mapping table; encoding the binary data group into corresponding base units according to the preset first mapping table; when the first binary bit of the binary data group is 1, obtaining a preset second mapping table; encoding the binary data group into corresponding base units according to the preset second mapping table; merging the corresponding base units of each binary data group to obtain a base sequence corresponding to the binary data, thereby improving the accuracy of DNA synthesis and sequencing.

Provided herein are compositions, devices, systems and methods for constructing and storing polynucleotides encoding information with redox resistant bases. The compositions, devices, systems, and methods described herein provide for storage or synthesis of a library comprising a plurality of polynucleotides with one or more redox resistance bases. Further provided herein are methods to increase DNA synthesis yield and fidelity.

This invention belongs to the fields of molecular biology and bioinformatics, specifically relating to a truncated bovine virus-encoded protein phage library, its construction method, and applications. This library covers all 162 known bovine viruses that host bovine subfamily viruses, containing 51,794 non-redundant peptide coding sequences of 56 amino acids each. These peptides are fused and displayed on the surface of the T7 phage capsid, with all coding sequences having a uniform total length of 200 bp. The library construction was achieved through sequence acquisition, truncation and redundancy removal, codon optimization and DNA synthesis, and in vitro ligation and packaging with T7 phage. The resulting library exhibits a positivity rate of 93% and a titer of 2.8 × 10⁻⁶. 10 PFU / mL. This invention features an optimized single-round high-throughput screening process adapted to clinical bovine serum samples, enabling efficient identification of bovine virus B-cell epitopes and providing tools and candidate targets for bovine virus vaccine development and diagnostic reagent development.

The invention relates to a high-flux enzymatic DNA synthesis method based on framework nucleic acid. Specifically, the invention provides a TdT-framework nucleic acid compound which can accurately regulate and control the position and distance of terminal deoxynucleotidyl transferase (TdT) so as to achieve the purpose of high-flux enzymatic DNA synthesis. The invention also provides a method for preparing the TdT-framework nucleic acid compound, and a method for synthesizing DNA (Deoxyribonucleic Acid) by using the TdT-framework nucleic acid compound. When the TdT-framework nucleic acid compound is used for synthesizing DNA, high-flux enzymatic DNA synthesis can be realized, and the TdT-framework nucleic acid compound has important significance for developing a high-flux DNA synthesis technology with mild conditions.

The invention relates to a DNA synthesis chip, a DNA synthesis method and application thereof, the DNA synthesis chip comprises: a first substrate, the first substrate is provided with a plurality of first grooves and a plurality of electrode groups, which are distributed at intervals; the electrode group comprises a first electrode and a second electrode; each first groove is in contact with one first electrode and one second electrode; the second substrate is provided with a reagent injection channel; the first substrate and the second substrate are configured to be combined with each other; when the first substrate and the second substrate are combined, the reagent injection channel is communicated with the first grooves, and a runner communicated with each first groove is arranged between the first substrate and the second substrate; the first substrate is provided with a first surface facing the second substrate. According to the DNA synthesis method disclosed by the embodiment of the invention, the difficulty of chip modification is reduced, the possibility of crosstalk is reduced, the synthesis amount is increased, and the chip can be repeatedly used, so that the synthesis cost is greatly reduced.

The invention relates to the technical field of DNA information storage, and discloses a method for prolonging data storage time and application, the method comprises the following steps: data is converted into a DNA sequence with a preset length, DNA is synthesized and stored, primary amino groups in basic groups of the DNA are independently modified by protective groups, the protective groups are selected from-COR, and the primary amino groups in the basic groups of the DNA are independently modified by the protective groups. R is selected from alkyl of C1-C6, substituted or unsubstituted naphthenic base of C3-C8 or substituted or unsubstituted phenyl. According to the method for prolonging the data storage time, the degradation rate of DNA can be remarkably inhibited, the stability of the DNA can be improved so as to realize long-time stable storage of the data, and the method is particularly suitable for forming a DNA storage scene with limited conditions, namely, the method is suitable for DNA synthesis in large companies with advanced technologies and equipment and also suitable for large companies with advanced technologies and equipment. The method is also suitable for DNA synthesis of small companies, and has application prospects and potential.

The invention relates to application of an active component in preparation of a medicine for treating tumor diseases. Specifically, it is found for the first time that small-molecule isovaleric acid and the prodrug thereof can inhibit DNA homologous recombination so as to efficiently inhibit tumor growth, and isovaleric acid and the prodrug thereof can be combined with chemotherapeutic drugs for radiotherapy, DNA damage induction or DNA synthesis inhibition to generate a synergistic tumor treatment effect.

The invention discloses a biosynthesis method and application of oligo pool. The biosynthesis method comprises the following steps: 1) fixing a probe, and combining a solid-phase carrier with a primer chain; (2) the solid-phase carrier is distributed as required, the solid-phase carrier or a deprotection product is distributed into M tubes as required according to the volume, and M is any natural number selected from 1, 2, 3 and 4; 3) chain extension: adding the reaction liquid into an enzymatic DNA synthesis system; 4) tube combination; (5) performing deprotection; (6) the steps (2)-(5) are repeated, the step (5) is executed N times in an accumulated mode, and N is selected from any one non-zero natural number; and 7) sampling, quality control and application. On the basis of a DNA (deoxyribonucleic acid) enzymatic synthesis technology, microspheres are used as solid-phase carriers to regulate and control the synthesis types of basic groups in each round so as to control the sequence diversity of the final oligo pool.

A method for DNA synthesis using protected nucleosides is disclosed. The nucleosides may be nucleoside triphosphates or nucleoside phosphoramidites with nucleobases attached to electrochemically-cleavable linkers. Removal of a protecting group by application of a voltage in solution triggers a cyclization reaction that cleaves the electrochemically-cleavable linkers. The electrochemically-cleavable linkers may include an amide linkage and an amide that forms a lactam or an ester linkage and a protected alcohol that forms a lactone when the protecting group is removed. The voltage used to cleave the electrochemically-cleavable linkers may be generated by activation of individual electrodes on a microelectrode array. The microelectrode array can be a substrate for solid-phase synthesis of oligonucleotides. Activation of specific electrodes removes the protecting groups at those electrodes and thus enables spatially-controlled extension of the oligonucleotides. Protected nucleosides linked to protecting groups by electrochemically-cleavable linkers are also disclosed.

The present invention relates to an in vitro cell-free process for production of deoxyribonucleotides (DNAs) comprising at least one hairpin, corresponding DNA products and uses thereof, and oligonucleotides and kits useful in the process of the invention.

The present disclosure relates to one or more agents, therapies, treatments, and methods of use of the agents and / or therapies and / or treatments for increasing production of a Cetuximab-like protein (CLP) by a subject that is administered the agent, therapy or treatment. Embodiments of the present disclosure can be used as a therapy or a treatment for a subject that has a condition that may benefit from reducing the DNA synthesis of genes that regulate cellular growth and proliferation.

The invention relates to a T7RNA polymerase mutant for synthesizing single-stranded DNA as well as a screening method and application of the T7RNA polymerase mutant, and belongs to the technical field of enzyme engineering. The invention provides a method for directed evolution and screening of a T7RNA polymerase mutant, which comprises the following steps: knocking out exonuclease and mismatch repair protein from a chassis strain genome, knocking in single-stranded annealing protein, resistance gene containing deletion mutation and a single-stranded DNA synthesis template, and constructing to obtain a genetically engineered bacterium for screening; a T7RNA polymerase mutant library is constructed, recombinant plasmids with the mutant library are transferred into engineering bacteria, T7RNA polymerase mutants synthesize single-stranded DNA to repair deletion mutation of resistance genes, the transformed engineering bacteria can grow on a resistance plate, the T7RNA polymerase mutants obtained through multiple rounds of screening can efficiently synthesize the single-stranded DNA through in-vitro verification, and the T7RNA polymerase mutant can be used for preparing the single-stranded DNA. A brand new tool is provided for synthesis of single-stranded DNA, and the method has the potential of being applied to the fields of gene editing, evolutionary engineering and the like.

The present invention provides a liposome, a preparation method thereof, and an application thereof in the preparation of a drug for treating PARP inhibitor-resistant breast cancer, relating to the field of biotechnology. A preparation method of a liposome provided by the present invention includes: injecting an ethanol solution dissolved with soybean phospholipid and cholesterol into an aqueous histidine solution for hydration under stirring conditions, volatilizing the ethanol component after the hydration ends, and then preparing the liposome after ultrasonic treatment. This preparation method is simple and convenient, and the encapsulation efficiency of the prepared liposome loaded with histidine is high. Through research by the inventor, it is found that histidine metabolic reprogramming occurs during the process of PARPi resistance in breast cancer cells, enabling the maintenance of DNA synthesis and repair. After treating PARPi-resistant breast cancer cells with the liposome of the present invention, the histidine content in breast cancer cells can be increased, making PARPi-resistant cells sensitive to PARPi, thereby realizing the treatment of PARPi-resistant breast cancer.

The present invention relates to the technical field of DNA synthesis, and specifically to a DNA synthesizer and a synthesis method, comprising: a nozzle printing device; a shower and air drying device; a protective device; a bottom plate printing device; and a positive pressure gas source and a negative pressure gas source, wherein the positive pressure gas source is connected to the nozzle and the air knife assembly, and the negative pressure gas source is connected to the exhaust pipeline, the liquid discharge hole and the nozzle. The present invention realizes the motion control and electric drive control of the nozzle printing device mechanism through the coordinated use of a motion control card, an electric drive controller, a positive pressure gas source and a negative pressure gas source. The object to be cleaned on the workstation can be cleaned, and the functions of air showering and air drying can be realized; the bearing mechanism has the function of automatically adsorbing the object to be cleaned, and can realize the fixation of the object to be cleaned; it can prevent the cleaning liquid from contaminating other workstations and equipment; it can realize the rapid recovery of waste liquid, quickly extract the waste liquid, and prevent it from accumulating on the bearing mechanism; and it can extract the residual gas, reduce the impact on the external environment, and prevent the waste liquid steam from polluting the working environment.

This invention relates to a dynamic scanning compensation method and DNA synthesis device based on inkjet printing, belonging to the field of DNA synthesis. By applying threshold segmentation, connected component analysis, and circular Hough transform to a standard analysis image, a circular region for each reaction site is obtained. Based on the distance of all edge points of each reaction site from the center of the circular boundary, it is determined whether each reaction site meets the roundness requirements, thus obtaining information on missing reaction sites. Simultaneously, to address potential inaccuracies after the circular Hough transform, the results are calibrated using the YOLO target detection model based on a convolutional neural network, achieving high-precision detection of missing reaction sites suitable for industrial applications. Furthermore, by adding recognition weights for defective reaction sites such as those with "partially excessive distances from the center" during YOLOv6 model training, the compatibility between the two is further improved, enhancing detection accuracy and meeting the requirements of high-precision DNA synthesis.

The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcribing DNA into RNA and an automated apparatus for RNA manufacturing. Further, the use of a bioreactor for RNA in vitro transcription as described herein is part of the present invention. The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP-compliant conditions. In particular, said RNA in vitro transcription reactor allows repetitive use of DNA template for various RNA in vitro transcription reactions. Further, the invention relates to an apparatus for RNA manufacturing comprising (a) a module for template DNA synthesis, (b) a module for transcribing DNA into RNA comprising said RNA in vitro transcription reactor, and, optionally, (c) a module for RNA formulation.

The invention provides a construction method for a tissue paraffin section space proteomics sequencing library. The method comprises the following steps: providing a solid phase carrier and an antibody-nucleic acid conjugate; attaching a tissue paraffin section sample to the solid-phase carrier, and carrying out section baking, dewaxing, hydration, decrosslinking and sealing treatment to obtain a sealed solid-phase carrier; incubating the antibody-nucleic acid conjugate and the closed solid-phase carrier to obtain a protein capture solid-phase carrier; carrying out permeabilization treatment and DNA synthesis on the protein capture solid phase carrier to obtain cDNA; releasing the cDNA from the solid-phase carrier, collecting the cDNA, and amplifying to obtain the sequencing library.

The application discloses an iron ion chelator based on adenine and synthesis and application thereof, and synthesizes a series of iron ion chelators with high affinity to ribonucleotide reductase, taking adenine as a leading compound, and the iron ion chelators have tridentate coordination donors (OH, N, N or N, N, N). In a salvage synthesis pathway, when the adenine derivatives as DNA synthesis raw materials contact with RR, the iron ions in the active center of the RR are effectively chelated, and then the activity of the RR is inhibited, the synthesis of DNA is blocked, and finally the proliferation of tumor cells is inhibited. The application reasonably utilizes the synthesis of DNA of cancer cells through a free base salvage synthesis pathway, and finds a new breakthrough for the iron ion chelator used for tumor treatment.

The present disclosure relates to methods, kits and products for barcoding of nucleic acids. In certain embodiments, the present disclosure provides a method of producing nucleic acid for sequencing utilising clonal amplification on a solid substrate, the method comprising: (a) providing a nucleic acid sample for sequencing; (b) amplifying the nucleic acid sample using an amplifying primer comprising a degenerate nucleotide sequence and a 5′ fixed nucleotide sequence, to produce amplified nucleic acids; and (c) further amplifying the amplified nucleic acids with (i) a first primer comprising the 5′ fixed nucleotide sequence and a first adapter nucleotide sequence, and (ii) a second primer comprising the 5′ fixed nucleotide sequence and a second adapter nucleotide sequence, wherein the first adapter nucleotide sequence or the second adapter nucleotide sequence provides a sequence for subsequent priming of DNA synthesis from a nucleotide sequence attached to the solid substrate and the other adapter nucleotide sequence provides a sequence for subsequent priming of DNA synthesis from a template produced from the subsequent priming, and wherein one or more of the first primer, the second primer and the amplifying primer comprise a specific identifier sequence to identify nucleic acids amplified with the first primer, the second primer and / or the amplifying primer; thereby producing nucleic acid for sequencing utilising clonal amplification on the solid substrate.

The application provides a nucleoside dimer, a preparation method thereof and application in DNA synthesis. The nucleoside dimer of the application adopts a mixed skeleton of methyl phosphate and beta-cyanoethyl phosphoramidite, a new preparation method of the nucleoside dimer is obtained by optimizing a synthesis method and a purification process, and a foundation for high-quality DNA synthesis is laid. On this basis, the nucleoside dimer with the new skeleton is applied to DNA synthesis, an oligonucleotide fragment synthesis method suitable for the dimer is established, and an oligonucleotide fragment with a length of 100 nt is synthesized. Fidelity experiment shows that the error rate of the DNA fragment synthesized by using the dimer is only 3.75 errors / Kb, and high-fidelity DNA synthesis is realized. The preparation method of the nucleoside dimer with the new skeleton can realize large-scale and high-purity preparation, the nucleoside dimer can play an important role in improving the length and fidelity of oligonucleotide synthesis, and has practical value.

A DNA synthesis method is provided. The DNA synthesis method according to some embodiments is a method for synthesizing a target DNA to be used as a data storage medium, and may comprise the steps of: preparing a template DNA; and synthesizing the target DNA from the template DNA on the basis of a DNA polymerase. Here, the sequence of the template DNA includes a universal base sequence, which can complementarily bind to any one of two or more types of data DNA bases, and a stop base sequence, wherein a starting base of the stop base sequence does not complementarily bind to the data DNA bases and can complementarily bind to a pre-designated non-data DNA base. According to this method, a target DNA having a desired sequence can be accurately and quickly synthesized in an eco-friendly manner.

Described herein are compositions and methods for polynucleotide synthesis. The composition for synthesis can comprise a nucleoside; and at least one protecting group, wherein the protecting group is configured for electrochemical removal under mild conditions. Methods include extending a polynucleotide, wherein the polynucleotide is extended by deprotecting a first protected nucleotide to generate a deprotected nucleotide. Methods may further include contacting the deprotected nucleotide with a second protected nucleotide thereby extending the polynucleotide.

In certain embodiments, the present disclosure provides kits for producing nucleic acids for sequencing utilizing clonal amplification on a solid substrate, including: an amplifying primer comprising a degenerate nucleotide sequence and a 5′ fixed nucleotide sequence; one or more target specific primers; (i) a first primer including 5′ fixed nucleotide sequence and a first adapter nucleotide sequence, and (ii) a second primer including 5′ fixed nucleotide sequence and a second adapter nucleotide sequence, wherein the first adapter nucleotide sequence or the second adapter nucleotide sequence provides a sequence for subsequent priming of DNA synthesis from a nucleotide sequence attached to the solid substrate and the other adapter nucleotide sequence provides a sequence for subsequent priming of DNA synthesis from a template produced from the subsequent priming, and wherein one or more of the first primer, the second primer and the amplifying primer includes a specific identifier sequence.

The invention discloses an electrochemical DNA synthesis and control method based on an updatable metal substrate, which comprises the following steps: preparing a liquid metal microelectrode or an electrode array, and carrying out electrochemical DNA synthesis on the surface of the electrode; after the phase change of the obtained electrode, the endocytosis of the DNA molecules on the surface of the electrode is realized by manually or automatically mechanically extruding the liquid metal, so that electrochemical synthesis and in-situ packaging are completed; the liquid metal oxide layer is removed to release internal molecules; after the liquid metal is extruded out, new liquid metal flows to the original position, or after the liquid metal is taken out of the array, new liquid metal is added in the original position of the array, and electrode or electrode array updating is achieved and used for a new round of synthetic reaction and molecular manipulation. The method provided by the invention is simple and efficient, can realize updating of the electrode for DNA synthesis and integrated molecular control, has extremely high expandability, and provides a new thought for further development of a DNA synthesis platform and practical application in the field of DNA data storage.

The present invention discloses the application of JH-RE-06 in the preparation of a drug for inhibiting the DNA synthesis of pseudorabies virus. The translesion DNA synthesis pathway inhibitor JH-RE-06 has the characteristics of a REV1 inhibitor and can induce the dimerization of REV1. The REV1-mediated TLS pathway is essential for the DNA synthesis of porcine pseudorabies virus. Therefore, JH-RE-06 can inhibit the proliferation of porcine pseudorabies virus. The present invention discovers for the first time the mechanism of action of JH-RE-06 in inhibiting the proliferation of pseudorabies virus and uses this drug for antiviral purposes for the first time.

The present invention discloses a multi-omics chip based on spatial interleaved coding, its preparation method, and its application. Based on 3D inkjet in situ DNA synthesis technology, the present invention utilizes a spatial interleaved coding strategy to produce a high-resolution, large-scale spatial multi-omics chip. This chip then uses a spatial in situ capture strategy to perform high-throughput in situ transcriptome analysis, in situ epigenomic analysis, in situ non-coding RNA analysis, and in situ proteome analysis on tissue sections. This achieves single-cell resolution and large-scale spatial coverage while maintaining a high gene count detection rate. The chip can be used to construct three-dimensional, fully covered, precise transcriptional maps or spatiotemporal maps of organs or tissues.