558 results about "Polyphenol oxidase" patented technology

The invention relates to a compound enzyme preparation for fermentation of pu'er tea, which is formed by compounding 10% to 20% of cellulase, 10% to 20% of pectinase, 5% to 15% of polyphenol oxidase, 5% to 10% of glucose oxidase, 5% to 10% glucoamylase, 5% to 10% xylanase, 5% to 10% of beta-glucanase, 5% to 10% of beta-mannase, 5% to 10% of tannase, 5% to 10% of acidic protease, 5% to 10% of lipase, 1% to 5% of alpha-amylase and 1% to 5% of phytase. By the aid of coordination of the enzyme system, polysaccharide and other substances in tea including cellulose and hemicellulose can be effectively decomposed, so that various physiochemical substances in cells can be dissolved. Meanwhile, oxidation and condensation of tea polyphenol, decomposition of protein, amino acid and carbonhydrate, a series of reactions of various products including polymerization and condensation and the like are accelerated and promoted.

The invention discloses a tobacco polyphenol oxidase gene NtPPO1 and a site-directed mutagenesis method and application thereof. The nucleotide sequence of the tobacco polyphenol oxidase gene NtPPO1 is shown in SEQ ID:No.1, the coded amino acid sequence is shown in SEQ ID:No.2. The invention further discloses a clone method of the tobacco polyphenol oxidase gene NtPPO1. The clone method comprisesthe specific steps that firstly, cDNAs of tobacco leaves are synthesized; secondly, PCR amplification of the NtPPO1 gene is conducted; thirdly, a CRISPR/Cas9 carrier of the NtPPO1 gene is constructed;fourthly, sequencing detection of the NtPPO1 gene mutation is conducted. The NtPPO1 gene has a wide application prospect in preventing tobacco browning. The tobacco polyphenol oxidase gene and the encoded protein thereof provide the gene and technology support for crops especially for anti-browning breeding of tobaccos.

A processing method of honeysuckle tea has a technical scheme that the method comprises the following steps of picking at regular time, bud grading, enzyme deactivation by steam, hot air drying, natural cooling, packaging and warehousing; the beneficial effect is that: honeysuckle buds are picked at 8:30-11:00 in the morning everyday, which can ensure that fresh flowers contain the most beneficial components and dew is prevented; grading of the buds can ensure uniform processing; enzyme deactivation by steam can rapidly inhibit the activity of polyphenol oxidase, and effectively prevent the decomposition of honeysuckle chlorogenic acid; direct hot air drying is performed after enzyme deactivation without cooling or cold air dehumidification, which can rapidly remove residual water contained in fresh flowers, destroys the activity of polyphenol oxidase, and allows the honeysuckle tea to be maintained to be undeteriorated for a long time; the honeysuckle tea processed by the method has a jade green color, and a lifelike shape; nutrient components can be maintained to be less lost; the infused tea has a bright and clear soup color, and a complete flower shape; the invention can greatly increase the drinking value and appearance quality of honeysuckle tea.

The invention discloses a method for preparing blueberry fruit wine and belongs to the technical field of deep-processing of agricultural products. The method comprises the following steps: juicing blueberries, performing instantaneous high-power pulse microwave treatment and composite pectinase enzymolysis, inoculating brewer's yeast, fermenting at controlled temperature, performing vacuum drum datomite filtration, composite flocculation clarification treatment, diatomite filtering, microfiltration and pulse microwave treatment and filling. According to the method, instantaneous high-power pulse microwave treatment is performed before fermentation, infectious microbes in the blueberry juice are killed under normal temperature conditions, polyphenol oxidase in the blueberry juice is passivated, sulfur dioxide is not required to be added, and pure fermentation is performed through inoculation of a good fermentation agent, so that high-quality blueberry fruit wine is produced, and the economic value of blueberries is obviously improved.

A method is provided for immobilizing a bioactive molecule onto a surface using polyphenol oxidase. In the presence of polyphenol oxidase, a bioactive molecule containing a phenol or catechol group can be simply in situ oxidized within a short time to dopa or dopaquinone which forms a coordinate bond with a metal or polymer substrate, thus immobilizing the bioactive molecule onto the surface with stability. Based on the surface immobilization of bioactive molecules using polyphenol oxidase, various bioactive molecules such as osteogenetic peptides and growth factors can be simply immobilized to medical metal or polymer substrate surfaces such as orthopedic or dental implants which can be then effectively used to induce rapid osteogenesis after being transplanted. Also, antithrombotic agents and/or entothelialization inducing agents may be immobilized to medical substrates for vascular systems, such as stents and artificial blood vessels, thus guaranteeing hemocompatibility to the medical substrates.

An additive of flue-cured tobacco is prepared from the protein degradation enzyme, starch degradation enzyme, pectin degradation enzyme, poly-phenol oxidase, cellulose, VC and potassium tartrate through adding them to water, stirring for dissolving and filtering to obtain the liquid product. It can be sprayed onto the leaves of tobacco to decreasing the content of organic high-molecular substances in tobacco leaf and improving its quality.

The invention relates to a composite biological enzyme preparation for the deweighting treatment of domestic sludge, and a production and application method thereof. The invention is characterized in that 100-500 kilograms of composite biological enzyme for the deweighting treatment of domestic sludge are used for 100 thousand tons of municipal domestic sewage, wherein the composite biological enzyme for the deweighting treatment of domestic sludge comprises 25-30 parts of protease, 20-25 parts of one or more of laccase, polyphenol oxidase and manganese peroxidase, 17-21 parts of one or more of xylanase, pentosanase and mannase, 20-25 parts of one or more of cellulase, pectinase and hemicellulase, 10-15 parts of lipase and 12-16 parts of amylase. Compared with the prior art, the invention removes hydrogen sulfide gas and undesirable odor thereof, simultaneously eliminates the inhibiting effect of H2S on the nitrification process, effectively reduces nitrate and ammonia, decomposes proteins, starch, fat, food oil, xylan, cellulose and other organic substances, converts suspended substances, accumulated waste and soluble organic substances in the sludge into carbon dioxide, nitrogengas, oxygen gas, water and sulphate, reduces the TSS (Total Suspended Solids), COD (Chemical Oxygen Demand) and BOD (Biochemical Oxygen Demand), greatly decreases the yield of the sludge, and has the advantages of no poison, no corrosiveness and no secondary pollution production.

The invention discloses an anti-browning method of milky lotus root juice and belongs to the technical field of fruit and vegetable food processing. The method comprises: cleaning lotus roots, peeling the lotus roots, removing the stem nodes of the lotus roots and slicing; adding a compound color fixative, blanching the sliced lotus roots for inactivating enzymes and adding water into the sliced lotus roots for pulping, wherein the compound color fixative added in the processing process can inhibit the complex browning reactions of the lotus roots in a high-temperature blanching process, the blanching can inactivate endogenous polyphenol oxidase in the lotus roots and inhibit enzymatic browning (the major cause of the browning of lotus root juice) of the lotus root juice in post-processing, storage and transport processes, and blanching can gelatinize the starch in the lotus roots to facilitate a follow-up enzymolysis process, destruct cell tissues and promote the dissolution of flavor substances; and adding fungalamylase for decomposing starch in the lotus root juice, obtaining mother lotus root juice for use in subsequent processing by rough filtration and ultrafiltration, and subjecting the lotus root mother juice to sugar and acid adjustment, sterilization and vacuum filling to obtain a lotus root juice drink. The lotus root juice drink keeps a desirable white index.

The invention discloses a processing method for low-caffeine and high-GABA (Gamma Aminobutyric Acid) black tea compounded with folium mori. The processing method comprises the following steps: dipping fresh tea leaves by hot water to remove caffeine in the tea leaves, spreading out and cooling the tea leaves, adding a defined amount of folium mori subjected to anaerobic and withering processing into the tea leaves, rolling the mixture, and performing fermentation by using polyphenol oxidase and peroxidase which are contained in the folium mori. For the prepared black tea, the content of the caffeine is smaller than or equal to 0.5%, and the content of the GABA is larger than or equal to 1.5 mg/g; during drinking, the black tea has fruity fragrance and tastes sweet, the liquor color is reddish yellow and bright, and the tea tastes mellow, not only has mellow fragrance of tea, but also has unique sweetness of the folium mori; the processing method is free from limitation of the picking time of the tea leaves.

The invention discloses a production method of wild honeysuckle flower tea. By adopting the production method, the original color and shape of wild honeysuckle flower can be kept unchanged and stable chlorogenic acid content can be ensured. The production method specifically comprises the following steps: picking at regular time; rapidly removing impurities; directly processing; carrying out rapid enzyme deactivation by high-temperature steam; rapidly dehumidifying by cold air; rapidly cooling by low-temperature cold air; rapidly drying by high-temperature hot air; naturally cooling and packaging; and warehousing for storage so as to guarantee quality. The production method has the beneficial effects of rapidly destroying activity of polyphenol oxidase in the wild honeysuckle flower, effectively avoiding decomposition of the beneficial substance namely chlorogenic acid contained in the wild honeysuckle flower, preventing the wild honeysuckle flower from browning, avoiding the procedures of turnover as well as pickup and placement, and ensuring that the appearance of the wild honeysuckle flower is not damaged and the flower shape is complete. The wild honeysuckle flower tea processed by the method has the advantages of green color, new shape and less loss of the chlorogenic acid; and the brewed wild honeysuckle flower tea has the advantages of light green and bright and clean color, delicious taste as well as complete and lifelike flower shape. Therefore, the drinking value and appreciation grade of the wild honeysuckle flower tea are obviously improved by adopting the production method.

The invention discloses a composite phosphate fruit and vegetable preservative and a usage method thereof. The preservative is compounded by the composite proportion of (0.8-1.2):(1.5-2.5):(0.05-0.15) of sodium pyrophosphate, citric acid and vitamin C. For the fresh-keeping of fruits such as freshly cut green apple, and the like, which contains polyphenoloxidase and chlorophyll, the preservative is compounded into the water solution with the massfraction of 1.0%-1.20% and the PH value of 2.0-3.0; according to the proportion of 100ml:100g of the solution and the freshly cut green apple, the freshly cut green apple is dipped into the solution at the temperature of 15-20 DEG C for keeping fresh; For the fresh-keeping of melons such as freshly cut potato, and the like, which contains polyphenoloxidase, the preservative is compounded into the water solution with the PH value of 5.5-6.0; according to the proportion of 100ml:100g of the solution and the freshly cut potato, the freshly cut potato is dipped into the solution at the temperature of 15-20 DEG C for keeping fresh. The preservative of the invention has dual efficacies such as browning prevention and greening prevention, affects no taste of the fruits and vegetables, has wide application range, simple and convenient operation and low expense and is generally suitable for the fruits and vegetables which are easy to be browned due to the promotion of the enzyme.

The invention discloses an extraction process of tea polysaccharide. The process includes: firstly washing fresh tea leaves with clean water, then performing microwave vacuum drying, then crushing the tea leaves, adding water into the obtained tea powder, and adding acid to adjust the pH value of the solution, then adding cellulase and polyphenol oxidase, carrying out hydrolysis under certain temperature, sending the obtained enzymolysis liquid into ultrasonic equipment to conduct ultrasonic treatment, then performing filtering and sending the filtrate into ultrafiltration equipment to conduct ultrafiltration treatment, then putting the ultrafiltrate into a centrifugal machine to conduct centrifugal separation, concentrating the obtained centrifugate, adding ethanol into the concentrated solution, carrying out centrifugal separation, and drying the tea polysaccharide, thus obtaining tea polysaccharide. By adopting the microwave low temperature vacuum drying and ultrasonic wave synergistic treatment technology, the process provided by the invention ensures effective wall breaking, and significantly improves the yield and antioxidant activity of polysaccharide.

The invention provides a method for preparing a high-purity theaflavin composite. The method comprises the following steps of: adding a mixture of green tea and catechinic acid into a crude polyphenol oxidase liquid for carrying out in-vitro oxidation; then, extracting the mixture with ethyl acetate; concentrating the extract in vacuum and drying to obtain a theaflavin crude product; dissolving the theaflavin crude product with ethanol water with volume concentration being 20% and then absorbing by using a chromatographic column with absorbing resin till the chromatographic column with absorbing resin is in a saturated state; eluting by using the ethanol water with volume concentration being 20%, and then eluting by using acetone or ethanol water with volume concentration being 60%; concentrating the eluting liquid, namely the collected acetone or ethanol water solution with volume concentration being 60% in vacuum; and obtaining the high-purity theaflavin composite through freeze drying. The method provided by the invention has the advantages of simple process and low cost and avoids using toxic and harmful organic reagents during a production process, thereby being environment-friendly. Furthermore, the high-purity theaflavin composite with different theaflavin components can be obtained by controlling different reaction conditions, so that the product yield is high and the content is high.

The invention discloses a technology for efficiently extracting radix puerariae starch and radix puerariae total isoflavone, comprising the following steps: breaking, draw filing, pH value adjustment and dipping, slag slurry separation, radix puerariae starch extraction, concentration, drying and radix puerariae total isoflavone recovery in process water; applying high pressure to the mixture obtained by draw filing to passivate polyphenol oxidase; adding cellulose enzyme to degrade the cellulose to ensure starch ionization rate; further setting the dipping time after the pH value of the slag slurry mixture is adjusted so as to not only ensure the full extraction of total isoflavone but also avoid character change of the radix puerariae starch due to improper dipping time; and further recovering the radix puerariae total isoflavone by the air flotation of dissolved air in the whole process so as to ensure the extraction rate of the radix puerariae total isoflavone. The whole process flow is simple, has easily controlled condition, protects environment, efficiently extracts radix puerariae starch and radix puerariae total isoflavone and greatly improves economic benefit.

The invention relates to seafood preservative and preparation and application thereof and belongs to the field of food preservation and fresh-keeping. The seafood preservative is prepared from carrageenan oligosaccharides and water, and the concentration of carrageenan oligosaccharides is 10-25 g/L. A preparation method includes the steps that firstly, the carrageenan oligosaccharides are prepared from carrageenan through an enzyme hydrolysis method, then the carrageenan oligosaccharides are used for preparing the preservation, specifically, the carrageenan oligosaccharides are dissolved in water, and the concentration of the carrageenan oligosaccharides in a solution is 10-25 g/L. The seafood preservative has the advantages that seafood can be effectively prevented from decaying, the total number of bacterial colonies of shrimps, the total volatile basic nitrogen (TVB-N), the Ph value, the activity of polyphenol oxidase, sensory indexes and other physiological and biochemical indexes are effectively improved, the shelf life of seafood is prolonged, and the preservative is in the food level and is safe and free of toxin.

The invention discloses a fresh-wet noodle color protecting method. The method includes the steps: performing microwave treatment for flour for 60-180 seconds before dough kneading, and controlling the discharge temperature at 75-85 DEG C so as to passivate polyphenol oxidase in the flour; adding starch acetate accounting for 5-8% of the mass of the flour, fumaric acid accounting for 0.03-0.06% of the mass of the flour and sterile water accounting for 33-40% of the mass of the flour during dough kneading, and performing vacuum dough kneading for 5-10min; performing composite rolling for kneaded doughs for three times on a noodle maker, and moisturizing and curing for 15-45min; rolling prepared dough strips into pieces on the noodle maker, and slicing so that wet noodles 2.0mm in width and 1.0mm in thickness are formed; and disposing the wet noodles under an ultraviolet lamp for sterilization for 20-40min, performing aseptic packaging and performing cold preservation for the packaged noodles at 4 DEG C. The fresh-wet noodles prepared by the method have the advantages that an L*value reaches 85.7-89.8, an a* value is decreased to 0.6-1.2 and a b* value is decreased to 13.1-14.2, while in a blank group, an L*value is 81, an a* value is 1.6 and a b* value is 17, and accordingly browning of the fresh-wet noodles is effectively inhibited, and the quality guarantee period of the fresh-wet noodles at the 4 DEG C reaches 20-28 days.

The invention provides a honeysuckle piece processing method. The technical scheme is that: the honeysuckle piece processing method comprises the following steps of cleaning and picking honeysuckle; deactivating the honeysuckle in steam; dehumidifying the honeysuckle in cool air; drying the honeysuckle in hot air; naturally cooling the honeysuckle; and packaging the honeysuckle for shipment. The honeysuckle piece processing method has the following beneficial effects that: activity of polyphenol oxidase can be quickly inhibited through steam deactivation; chlorogenic acid of the honeysuckle can be effectively prevented from decomposition; water content attached to surfaces of the honeysuckle can be removed through the dehumidification in cool air, so that drying speed can be accelerated; remaining water content contained in fresh flower can be quickly removed through the drying in hot air; activity of the polyphenol oxidase can be broken; and packaged honeysuckle medicine (pieces) can be preserved in a storage room for a long term without going bad.

A Chinese gooseberry clear juice concentrating process comprises the steps of shedding, cleaning, crushing, beating and separating of Chinese gooseberry to obtain Chinese gooseberry primary pulp, and also comprises the following steps: the Chinese gooseberry primary pulp is processed by zymohydrolysis, polyphenol oxidase inactivation, horizontal screw separation, clarification, hyperfiltration, decolorization and concentration to obtain the concentrated Chinese gooseberry clear juice. The invention solves the difficulties on the aspects of fruit pulp separation, clarification and decolorization of the Chinese gooseberry clear juice production, realizes the massive production, also integrally adopts the UHT sterilization technology and the temperature reduction technology so as to better ensure the quality index of the sterilized Chinese gooseberry clear juice and remain the nutrient content rich in Chinese gooseberry to the maximum, including juice color, flavor, nutrient content, and the like.