119 results about "Mannoglucan" patented technology

The invention relates to the technical field of functional food, in particular to immune regulation type prebiotic syrup with synergy of galactomannan. The active components of the composite solid beverage are prepared from the following raw materials in percentage by weight: 20%-35% of fructo-oligosaccharide, 10%-18% of galactooligosaccharide, 8%-15% of stachyose, 3%-7% of xylooligosaccharide, 5%-12% of lactitol, 5%-10% of galactomannan, 2%-5% of yeast beta-glucan and 1%-3% of N-acetylglucosamine. The composition further comprises 4-8% of inulin and 3-6% of resistant dextrin as a matrix filling and slow-release carrier, and the sum of the weight percentages of all the active ingredients and the carrier is 100%. By remodeling intestinal microecology and inhibiting inflammation pathways, the intestinal inflammation state can be remarkably improved, expression of intestinal mucosa tight junction protein is promoted, and an innovative solution is provided for people suffering from chronic bowel diseases and immune imbalance.

The invention belongs to the technical field of food processing, and particularly relates to a product rich in soluble yeast dietary fibers and a preparation method and application thereof. Wherein in terms of dry matter mass of the product containing the soluble yeast dietary fiber, the product containing the soluble yeast dietary fiber comprises the following components in percentage by mass: more than 40% of soluble yeast dietary fiber, less than 0.5% of fat, more than 20% of yeast beta-glucan and more than 20% of mannan. The product containing the soluble yeast dietary fibers prepared by the preparation method disclosed by the invention contains the soluble dietary fibers with higher content, the solubility is obviously improved, the mouth feel is improved to a certain extent, and the product contains lower fat content and is more beneficial to storage; and meanwhile, the product is rich in beta-glucan and mannan, so that the product has a wide application prospect.

The invention relates to the technical field of microorganism application, in particular to low-nucleic-acid yeast protein as well as a preparation method and application thereof. The preparation method of the low-nucleic-acid yeast protein comprises the following steps: (1) carrying out inactivation treatment on high-protein yeast; (2) carrying out enzymolysis on the inactivated yeast by adopting neutral protease, glucanase, mannase and first nuclease, and after enzyme deactivation, separating and taking a heavy phase to obtain crude yeast protein; (3) performing high-pressure homogenization treatment on the crude yeast protein; and (4) carrying out enzymolysis on the crude yeast protein subjected to high-pressure homogenization treatment by adopting second nuclease and lipase, and after enzyme deactivation, separating and taking a heavy phase to obtain the low-nucleic-acid yeast protein. The yeast protein with the nucleic acid content of 1.0% or below is obtained by taking the high-protein yeast as a raw material through inactivation, compound enzyme enzymolysis, high-pressure homogenization treatment and secondary enzymolysis, is neutral and pure in taste, and can be widely applied to the fields of nutrition, health and food such as protein supplementation and protein substitution.

To provide konjak-like food with high protein content, while utilizing the advantages of konjac, which has a unique texture and a low caloric value.SOLUTION: A liquid mixture contains 0.3 w / w% or more and 2.5 w / w% or less of glucomannan, 0.2 w / w% or more and 2.5 w / w% or less of alginic acid and / or a salt thereof, and 3.0 w / w% or more and 12.0 w / w% or less of vegetable protein, optionally containing 0.1 w / w% or more and 1.5 w / w% or less of xanthan gum, and substantially free of gluten. The liquid mixture is discharged into an aqueous solution containing divalent cations to produce a gel-like product.SELECTED DRAWING: None

The present invention relates to the technical field of clinical medicine immunodetection, and discloses an aspergillus galactomannan detection kit and a preparation method thereof, the aspergillus galactomannan detection kit comprises a test paper card and a sample treatment liquid, the test paper card comprises a bottom plate, and a sample pad, a labeling pad A, a labeling pad B, a nitrocellulose membrane and water absorption paper which are sequentially arranged from a sample adding end on the surface of the bottom plate, the labeling pad A is provided with a quantum dot-biotin labeled aspergillus galactomannan monoclonal antibody, the labeling pad B is provided with microspheres coupled with the aspergillus galactomannan monoclonal antibody, and the nitrocellulose membrane is coated with streptavidin and goat anti-mouse IgG. According to the invention, the defect of low sensitivity can be overcome.

The application discloses a high-stability immobilized mannanase and a preparation method and application thereof, and belongs to the technical field of enzyme immobilization. The preparation method uses PAMAM modified SBA-15 / calcium alginate composite microspheres as a carrier to fix mannanase in an enzyme solution by adopting an embedding-covalent crosslinking method, so as to obtain the high-stability immobilized mannanase. The preparation method uses the PAMAM modified SBA-15 / calcium alginate composite microspheres as the carrier to fix the mannanase by adopting the embedding-covalent crosslinking synergistic method, so that the pH tolerance, the circulation stability and the hypoglycemic efficiency of the enzyme are improved, and the problems of the traditional immobilized enzyme, such as large enzyme activity loss, easy leakage and poor stability under high temperature, are solved.

To provide a tablet that is easy to swallow and also has reduced production cost, and a method of producing the same.SOLUTION: A tablet has a naked tablet containing a gelator, and at least one selected from sugar alcohol, monosaccharide, disaccharide, oligosaccharide, cellulose, cellulose derivative, starch, starch derivative and starch decomposition product. Preferably, the gelator is at least one selected from xanthan gum, locust bean gum, guar gum, mannan, glucomannan, hyaluronan, agar, alginic acid, tamarind gum, psyllium seed gum, tara gum, carrageenan, acacia gum, gum arabic, ghatti gum, tragacanth gum, karaya gum, cassia gum, rhamsan gum, welan gum, macrophomopsis gum, curdlan, pullulan, gellan gum, pectin, and soybean polysaccharide, gelatin, collagen, polyglutamic acid, polylactic acid, polyglutamic acid and the like.SELECTED DRAWING: None

[Problem] To provide a composition for regulating blood pressure for a healthy person. [Solution] A blood-pressure-regulating composition for a healthy person, said composition being characterized by containing a guar gum degradation product, and being characterized in that: 70 mass% or more of the guar gum degradation product has an average molecular weight within the average molecular weight range of 1.0 × 103 to 1.0 × 105; the composition has a viscosity of 10 mPa·s or less when a 2 mass% aqueous solution at 37°C is measured at 10 rpm using an E-type viscometer, or has a viscosity of 5 mPa·s or less when the aqueous solution is measured at 20 rpm, or has a viscosity of 3 mPa·s or less when the aqueous solution is measured at 30 rpm; the composition is obtained by hydrolyzing a galactomannan polysaccharide which contains guar-derived endosperm and in which the content ratio (galactose:mannose) of galactose and mannose is within the range of 1:1.3 to 1:2, by using a microorganism-derived β-mannanase to reduce the molecular weight of the polysaccharide; and the composition contains at least 70 mass% of dietary fiber in terms of the dietary fiber content stipulated in the enzyme-HPLC method.

The invention relates to a β-glucan-rich Saccharomyces cerevisiae yeast cell wall extract for use in the prevention or treatment of a disease associated with or caused by a Lawsonia intracellularis infection in pigs, characterised in that the extract comprises β-glucans and mannans, where the β-glucans are present in the form of a mixture of β-1,3-glucans and β-1,6-glucans in a total amount of greater than or equal to 50% by weight, notably of between 50% and 99% by weight, and where the mannans are present in a total amount less than 5% by weight, notably of between 1% and 5% by weight.

The utility model discloses an adjustable partition structure for mannan storage, which belongs to the technical field of mannan storage, and adopts the technical scheme that the adjustable partition structure comprises a box body, a plurality of adjusting components are movably connected in the box body, a plurality of partition components are arranged at the tops of the adjusting components, and each partition component comprises a movable upright post; the problems that an existing storage device is poor in separation effect, mannan cannot be stored according to different structures, mannan of different structures is possibly different in stability, solubility and the like due to the fact that the property of mannan is closely related to the structure of mannan to a certain degree, and mannan cannot be effectively separated, and the storage effect is poor are solved. The problems that in the prior art, due to the fact that the mannan can be mutually influenced in the storage process, for example, part of mannan is affected by damp or other impurities and deteriorates, chain reaction is possibly caused, the quality of the whole batch of mannan is affected, and the use convenience of the device is reduced are solved.

The invention discloses highly acetylated glucomannan and application thereof in preparation of a product with an effect of relieving prediabetes mellitus, and belongs to the technical field of medicines. In-vivo experiments prove that the glucomannan with high acetylation degree has the effect of relieving the prediabetic stage, which is specifically reflected in weight gain of mice in the prediabetic stage, regulation of blood glucose metabolism, improvement of glucose tolerance and insulin resistance, regulation of blood lipid metabolism, protection of liver and pancreatic tissues and reduction of epididymal fat cell size. The invention provides an important basis for developing a dietary formula suitable for prediabetic patients, and provides a new method for prevention and management of diabetes mellitus.

The present application relates to the technical field of genetic engineering and protein engineering, and particularly provides a mannanase mutant and application thereof.The mutant is obtained by mutating the 175th amino acid of mannanase with the amino acid sequence of SEQ ID NO:4 from Ser to Thr, and the specific activity of the mutant is significantly higher than that of the wild type, so that the mutant can be widely applied to the production of livestock and poultry feed and marine aquaculture feed.

This invention discloses a composition that can effectively soothe and repair sensitive skin, its preparation method, and its application, relating to the field of skincare products. The composition of this invention, by weight, comprises 5-18 parts β-glucan, 1-10 parts mannan, 8-15 parts yeast polypeptides, 1-10 parts sodium hyaluronate, 2-5 parts magnesium ascorbate phosphate, and 8-15 parts Scutellaria baicalensis root extract. This composition is mild and non-irritating, can quickly and effectively relieve skin sensitivity reactions, protect the skin barrier, and has good storage stability, not easily affected by environmental factors such as temperature and oxygen.

A mannanase at least 75% identical to SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4, a poly-nucleotide encoding mannanase, an expression construct comprising the polynucleotide, and a host cell comprising the polynucleotide or the expression construct.

This invention belongs to the field of agricultural fertilizer technology, specifically relating to an organic water-soluble fertilizer based on molasses fermentation concentrate and its preparation method. The organic water-soluble fertilizer comprises molasses fermentation concentrate, a compound enzyme preparation, polyaspartic acid, EDTA-2Na, and water-soluble nitrogen, phosphorus, and potassium fertilizer. It employs a staged temperature-varying enzymatic hydrolysis process. Laccase first oxidizes and degrades polyphenols at 30–35°C, opening the polyphenol-polysaccharide complex structure. Then, the temperature is raised to 42–48°C, where β-glucanase, xylanase, and mannanase directionally degrade colloidal polysaccharides. Simultaneously, EDTA-2Na is introduced to chelate metal ions, and polyaspartic acid inhibits scale dispersion. These three components synergistically form an "enzymatic hydrolysis-chelation-scale inhibition" anti-clogging system. This invention addresses the technical problems of existing molasses fermentation concentrate fertilizers being prone to clogging in drip irrigation systems, having low efficiency in constant-temperature synchronous enzymatic hydrolysis, and having an imperfect single anti-clogging mechanism. It provides an organic water-soluble fertilizer composed of molasses fermentation concentrate, a compound enzyme preparation, polyaspartic acid, EDTA-2Na, and water-soluble nitrogen, phosphorus, and potassium fertilizer. This invention effectively solves the problem of clogging of honey fermentation concentrate in drip irrigation systems, improves enzymatic hydrolysis efficiency, and is suitable for drip irrigation fertilization of various crops such as bananas and vegetables.

The present invention relates to newly idenified GH26 mannanases and their use as enhancers of enzyme benefits in detergents as well as a detergent composition comprising said mannanases.

The present invention relates to the technical field of biology, and in particular to a β-mannanase mutant M-Q7S having a high enzyme activity, a recombinant strain, and a use of the mutant. The single-site β-mannanase mutant Csman5A-Q7S is obtained by mutating into serine (S) the amino acid asparagine (Q) at position 7 of the β-mannanase having the amino acid sequence as shown in SEQ ID NO: 1. Experiments show that the specific enzyme activity of the mutant Csman5A-Q7S in the whole induction period is significantly greater than that of the original Csman5A, and after 144 h of induction, when the specific enzyme activity reaches the maximum value, the specific enzyme activity of the mutant Csman5A-Q7S is 5.4 times that of the original Csman5A.

Disclosed herein are mannan nanogels as a novel vaccine delivery platform as well as a novel method of making a self-assembling mannan nanogel for in vivo delivery of therapeutic agents.

The present invention provides a functional paper product which has water resistance, oil resistance and can be recycled. A functional paper product (1) according to the present invention comprises a base paper (20) and a coating film (10) formed on the base paper (20), the coating film (10) having, as a main component, modified mannan in which acetyl groups in konjac mannan are free, the coating film (10) being obtained by curing a swollen modified mannan sol in a state in which the swollen modified mannan sol is immersed in gaps between cellulose fibers of the base paper (20), and the viscosity of the swollen modified mannan before curing is 3000 mPa * s or less.

The present application provides a soluble dietary fiber composition, its preparation method and application. After the soluble dietary fiber composition enters the colon, the ester bond in the alanine modified pectin is rapidly degraded, part of the chlorogenic acid is released, the secretion of anorectic hormone is stimulated, the appetite is reduced, and the heat intake is reduced; the remaining amide bond network is slowly degraded, realizing the slow and continuous release of chlorogenic acid. Lutein removes free radicals generated by fat decomposition, reducing the oxidative damage of L cells. Galactomannan and alanine modified pectin-glucan modified protein-chlorogenic acid ternary complex synergize to improve the molar ratio of butyric acid / propionic acid: propionic acid enhances satiety through intestinal gluconeogenesis, and butyric acid stimulates the secretion of GLP-1 / PYY, realizing endogenous appetite suppression. Galactomannan absorbs water to form a lubricating gel, softening the stool and increasing the stool volume; the ternary complex degrades to produce short-chain fatty acids, optimizing the intestinal microenvironment and enhancing gastrointestinal peristalsis, both of which synergistically improve constipation.

This application discloses a muscle stem cell resuscitation and protection washing solution, a quality assessment kit, and a method of use, belonging to the field of cell biology technology. In this application, vitamin E succinate and mannan peptide are used as core antioxidant components, synergistically combined with Gynostemma pentaphyllum polysaccharide, Portulaca oleracea polysaccharide, trehalose, L-proline, and magnesium sulfate heptahydrate to construct a four-in-one resuscitation and protection washing solution that provides isotonic buffering, extracellular protection, intracellular protein stabilization, antioxidant effects, and mitochondrial stabilization. This increases the survival rate of muscle stem cells after resuscitation to over 93%. The kit integrates a multi-dimensional detection system for cell resuscitation and protection washing solution, cell viability, proliferation rate, Pax7 / MyoD double-positive stemness assessment, and cryopreservation damage, achieving closed-loop management of the entire process of muscle stem cell resuscitation, protection, and quality assessment. The double-positive rate of stemness markers reaches over 90%, providing reliable quality assurance for cell bank management and clinical applications.

The application discloses a kind of feed additive and its application, it is related to feed additive technical field.The prepared feed additive of the application is by mixing sodium glucuronate, glucomannan, dimethyl cyclodextrin, diatomite and sodium lauryl sulfate, then mixed solution is reacted with calcium chloride crosslinking solution, finally form microspheres type feed additive.Compared with prior art, the prepared feed additive of the application is beneficial to the adsorption of deoxynivalenol and zearalenone, amino acid liquid, lemon yellow and titanium dioxide are mixed, then composite bacteria liquid is added to ferment, by feed additive processing, low-temperature drying can retain the nutrient components and biological activity in raw materials, and the biological fermentation protein raw material with good sensory color is obtained.

The present invention aims to provide an intraoral adhesive film that combines adhesive properties and slow dissolution properties in a well-balanced manner. [Solution] The present invention provides an intraoral adhesive film containing (A) ιcarrageenan and (B) κcarrageenan. Preferably the film further contains (C) a water-soluble polymer such as tamarind gum, gum arabic, karaya gum, xanthan gum, gellan gum, tragacanth gum, guar gum, tara gum, locust bean gum, pullulan, gelatin, casein, pectin, agar, glucomannan, galactomannan, starch, carboxymethyl starch, dextrin, alginic acid, alginic acid ester, chitin, chitosan, carboxymethylcellulose, λcarrageenan, hydroxyethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, methylcellulose, and ethylcellulose.

An aqueous cosmetic composition for keratin fibers comprising: a) at least one acetylated or non-acetylated glucomannan polymer, b) at least one linear or branched, saturated or unsaturated C12-C22 fatty alcohol, c) at least one conditioning agent.

The present invention relates to a yeast-derived polysaccharide that induces Treg cells and its use, and more particularly to a polysaccharide containing mannan and β-glucan, an immunomodulatory composition comprising the polysaccharide as an active ingredient, a pharmaceutical composition or food comprising the polysaccharide as an active ingredient for preventing or treating an immune disease or inflammatory disease, a method for preparing regulatory T cells by using the polysaccharide, a cell therapy product comprising regulatory T cells prepared by the preparation method as an active ingredient, and a method of treating using the cell therapy product. Even at low doses, the novel polysaccharide according to the present invention allows the production of tolerogenic antigen-presenting cells by retaining the β-glucan and mannan structures therein, and the novel polysaccharide is thereby capable of inducing the differentiation or production of antigen-specific regulatory T cells (Treg cells), thereby regulating the target immune system with low adverse reactions. Therefore, MGCP and the Treg cells induced by the polysaccharide effectively prevent or treat immune diseases or inflammatory diseases.

The invention belongs to the field of food, and particularly relates to a yeast enzymatic hydrolysate, a yeast protein and a yeast protein sodium salt product with high emulsifying performance as well as preparation and application of the yeast enzymatic hydrolysate and the yeast protein sodium salt product. The emulsifying activity of the yeast enzymatic hydrolysate is greater than or equal to 25.0 m / g, and the emulsifying stability is greater than or equal to 77.0%; the emulsifying activity of the yeast protein is greater than or equal to 70.0 m / g, and the emulsifying stability is greater than or equal to 175.0%; the emulsifying activity of the yeast protein is greater than or equal to 70.0 m / g, and the emulsifying stability is greater than or equal to 175.0%. According to the preparation method disclosed by the invention, the yeast is subjected to composite enzymolysis through the alkaline protease, the flavourzyme, the glucanase, the mannase and the glutamine transaminase and then is subjected to ionization reaction with the sodium hydroxide to obtain the yeast protein sodium salt product, and the yeast protein sodium salt product has relatively high emulsifying activity and also has relatively good color and flavor; the sodium caseinate can be used as a natural emulsifier, and the problems of limited sodium caseinate yield, unstable supply and high price are solved.

The invention relates to the field of traditional Chinese medicines, and discloses radix tetrastigme polysaccharide, a preparation method thereof and application of the radix tetrastigme polysaccharide in preparation of antithrombotic drugs. The novel acid glucuronic acid galactomannan (THP5) is extracted from the overground part of the radix tetrastigme polysaccharide for the first time, it is found that the novel acid glucuronic acid galactomannan (THP5) can be used for preventing and treating thrombus diseases including arterial thrombosis, venous thrombosis and the like in a multi-target mode, the application range of the radix tetrastigme polysaccharide is widened, and a new way is provided for treating the thrombus diseases.

The invention belongs to the technical field of biological medicines, and relates to a superoxide dismutase / mu-conotoxin sustained-release compound based on a galactomannan covalent shell and application of the superoxide dismutase / mu-conotoxin sustained-release compound. Galactomannan in the compound is oxidized by NaIO4 and is coupled with stearic acid to form a stearic acid-oxidized galactomannan shell-core structure, mu-conotoxin is connected with galactomannan through a covalent bond, superoxide dismutase is loaded into the shell-core structure, and the structural formula of the compound is as follows: SA-O-CM-mu-CnIIICn / SODm. Wherein n is a natural number from 3 to 11, and m is a natural number from 1 to 8. The SOD and the mu-CnIII C are loaded into the galactomannan covalent shell layer, so that the stability and the biological activity of the SOD and the mu-CnIII C can be effectively improved, degradation of hydrolase on protein and polypeptide is resisted, and the compound is endowed with excellent antioxidant, anti-wrinkle, anti-inflammatory and anti-radiation activity.

The purpose of the present invention is to provide an intraoral adhesive film which has a good balance of adhesive properties and sustained-release properties. Specifically, the present invention provides an intraoral adhesive film containing (A) ι carrageenan and (B) κ carrageenan. It is preferable that the film further contain (C) a water soluble polymer such as tamarind gum, gum arabic, karaya gum, xanthan gum, gellan gum, tragacanth gum, guar gum, tara gum, locust bean gum, pullulan, gelatin, casein, pectin, agar, glucomannan, galactomannan, starch, carboxymethyl starch, dextrin, alginic acid, alginic acid ester, chitin, chitosan, carboxymethyl cellulose, λ carrageenan, hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, methyl cellulose, or ethyl cellulose.

The invention relates to the technical field of oil and gas development materials, and particularly discloses a gel breaking temporary plugging agent and a preparation method thereof. The gel breaking temporary plugging agent is prepared from the following raw material components: beta-mannase, a calcium salt solution, ammonium persulfate microcapsules, oxidation modified bacterial powder, silicon dioxide microspheres, beta-mannan-based gel and water. The gel breaking temporary plugging agent provided by the invention effectively solves the problems of incomplete gel breaking, easy generation of secondary plugging and poor controllability of gel breaking time of a traditional temporary plugging agent.