321 results about "Mannoglucan" patented technology

The invention relates to a compound enzyme preparation for fermentation of pu'er tea, which is formed by compounding 10% to 20% of cellulase, 10% to 20% of pectinase, 5% to 15% of polyphenol oxidase, 5% to 10% of glucose oxidase, 5% to 10% glucoamylase, 5% to 10% xylanase, 5% to 10% of beta-glucanase, 5% to 10% of beta-mannase, 5% to 10% of tannase, 5% to 10% of acidic protease, 5% to 10% of lipase, 1% to 5% of alpha-amylase and 1% to 5% of phytase. By the aid of coordination of the enzyme system, polysaccharide and other substances in tea including cellulose and hemicellulose can be effectively decomposed, so that various physiochemical substances in cells can be dissolved. Meanwhile, oxidation and condensation of tea polyphenol, decomposition of protein, amino acid and carbonhydrate, a series of reactions of various products including polymerization and condensation and the like are accelerated and promoted.

The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo-β-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.

The invention discloses acid beta-mannase, genes, engineering bacteria and a structure thereof. A coding gene manAsp is from Aspergillus niger CBS 513.88, the coding gene has a nucleotide sequence expressed as SEQ ID NO.3 or 4, and the acid beta-mannase obtained by coding has an amino acid sequence expressed as SEQ ID NO.1 or 2. The structure of the engineering bacteria is obtained by guiding a pichia pastoris constitutive expression vector (pGAPZa-man) containing the coding gene sequence of the beta-mannase into pichia pastoris. The acid beta-mannase of the invention has the following properties: at the optimal pH of 5.0 and the optimal temperature of 40 DEG C, the beta-mannase has better stability when the pH is between 3.0 and 7.0, and the residual enzyme activity is 66.39 percent when the beta-mannase is treated for 1 hour at the temperature of 90 DEG C; the engineering bacteria achieve efficient expression of the acid beta-mannase; and the fermentation process is simple, has low extracting cost, is suitable for large-scale industrialized production, and has broad application prospect in the industries of feed, food, medicine, energy and the like.

The invention discloses a piglet feed complex enzyme preparation and a production method and application thereof. The complex enzyme preparation comprises the following enzyme activity components: 80-100U/g of glucose oxidase, 20-25U/g of alpha-galactosidase, 3000-4000U/g of xylanase, 500-600U/g of beta-dextranase, 500-600U/g of beta-mannase, 2000-3000U/g of acid proteinase, 800-1000U/g of neutral protease, 800-1000U/g of medium-temperature alpha-amylase, 400-500U/g of fungus alpha-amylase and 3000-4000U/g of glucamylase. According to the complex enzyme preparation, a mixture of corn starch, monopotassium phosphate, dipotassium phosphate and calcium sulfate serves as a diluent. The using method of the complex enzyme preparation comprises the step of adding 500-750g of complex enzyme preparation into every ton of piglet complete formula feed.

The invention provides a pig feed complex enzyme preparation and belongs to the field of feed additives. The additive is composed of a complex enzyme and a fortifier, wherein the complex enzyme is the combination of lipase, neutral protease, beta-amylase, isoamylase, neutral phytase, beta-mannase, xylanase and alpha-galactosidase, and the fortifier is composed of pine needle extracts, bamboo leaf crude polysaccharide, honeysuckle stem powder, galangal powder, common vladimiria root powder, lithospermum powder, vitamin C, sodium chloride, sodium butyrate, ferrous sulfate and calcium gluconate. By the adoption of the enzyme preparation, the utilization rate of feed can be effectively increased, the feed conversion ratio can be reduced, the growing speed of pigs can be increased, the immunity of pigs can be improved, the health degree of pigs can be increased, the morbidity of pigs can be reduced, and market prospects are quite broad.

The invention relates to an exogenous composite enzyme preparation special for feed industry and a preparation method and application thereof. The composite enzyme preparation comprises five kinds of water-soluble non-starch polysaccharide enzyme and phytase, namely xylanase, dextranase, mannose, cellulase and pectase. The activity mixture ratio of the contained water-soluble non-starch polysaccharide enzyme to the contained phytase is designed according to the kinds and the contents of the non-starch polysaccharide and the phytase in raw materials of a common feed, the synergistic effect between two kinds of the enzymes can be fully achieved, and the most scientific and economic mixture ratio is provided. The exogenous composite enzyme preparation can improve the degradation efficiency of non-starch polysaccharide anti-nutritional factors and phytic acid in the feed, remove the anti-nutritional effect thereof, improve the absorption and conversion rate of the feed and the utilization rate of phosphorus in the feed, substantially reduce the dosage of inorganic phosphorus and the production cost, bring considerable economic benefit to feed processing enterprises and farmers, greatly reduce the contents of nitrogen and phosphorus in animal excrement so as to reduce environmental pollution, and has remarkable social benefit.

The invention belongs to the field of biological feed, and relates to a high-efficiency and low-cost fermentation feed processing method based on bean dregs and application; the processing method comprises the steps of activating a strain to obtain fermentation seed liquid of bacillus, yeast and lactic acid bacteria; mixing the bean dregs with bran or rice bran, then adding sucrose, adding cellulase, cellobiase, glucoamylase, xylanase, mannanase and beta-glucanase, mixing to obtain a primary aerobic fermentation medium, inoculating the bacillus fermentation seed liquid and the yeast fermentation seed liquid for primary aerobic fermentation, obtaining a primary fermentation mixture after fermentation, and then inoculating the lactic acid bacteria fermentation seed liquid for anaerobic fermentation under sealed conditions to obtain a finished product; an bacteria-enzyme cooperative fermentation and aerobic and anaerobic step-by-step fermentation mode is adopted, the fermentation is completed, the conversion degree of soybean peptide is high, the removal rate of trypsin inhibitor is 85% or above, each mycotoxin is lower than the feed hygiene limit standard of China; the feed is good in palatability, and favored by animals, and the economic efficiency is high.

The invention provides a Beta-mannose which has amino acid sequences shown in SEQ ID: NO.2, SEQ ID: NO.4, SEQ ID: NO.6 and SEQ ID: NO.8. Or substitution, depletion or addition of one or multiple amino acids is carried out to the amino acid sequences shown in the SEQ ID: NO.2, SEQ ID: NO.4, SEQ ID: NO.6 or SEQ ID: NO.8 to obtain amino acid sequences of Beta-mannose with the same activity. The invention also provides a gene for coding the Beta-mannose, a recombinant vector containing the gene and a host cell containing the recombinant vector. The invention further provides a preparation method of the Beta-mannose, including the culture of the host cell provided by the invention. The Beta-mannose provided by the invention has heat-resisting property and acid resistance and a prokaryotic expression system established by the invention can be utilized for production. Furthermore, six histidine tags can be utilized for purification.

The invention provides a method for preparing high-purity oligosaccharides by the enzymolysis of hemicellulose containing mannosan, and a product which is a high-effect beta-mannase BM-II produced by fermenting bacillus subtilis TQBm with the preservation No. of CCTCC No: M 211147. Konjac glucomannan, guar gum, sophora bean gum, sesbania gum and other hemicelluloses containing the mannosan are hydrolyzed into relatively small molecule materials, and then are desalted, decolored and deodorized through centrifugal separation, flocculation clarification and ion exchange techniques, large molecule materials in sugar solution are separated by the combination of an ultrafiltration technique and a molecular sieve chromatography technique, and finally the high-purity oligosaccharides is prepared through concentration and drying. The method for preparing the high-purity oligosaccharides disclosed by the invention has the advantages of simple operation, good separation effect, low cost and no chemical pollution, and industrialized production can be realized. The oligosaccharides product disclosed by the invention can obviously proliferate bifidobacteria in the human intestinal tract and otherbeneficial bacteria and obviously reduce enterobacter and other harmful bacteria, has the functions of blood sugar and blood fat reduction and immunoregulation, and is a novel raw material and assistant for gastrointestinal health, blood sugar and fat reducing, immunoregulation and disease resisting and preventing medicines.

The invention relates to a meat duck feed containing vinegar residues and belongs to the technical field of stock raising feed production. The meat duck feed comprises vinegar residues and complex enzyme preparations, wherein the complex enzyme preparations include cellulose 1200-1800U, xylanase 25000-35000U, mannose 40-60U, protease 8000-12000U, pectinase 180000-250000U, glucanase 3000-5000U, the balance being carriers, and the carriers are dehydrated corncob powder. According to characteristics of vinegar residues, reasonable varieties and ratio of exogenous digestive enzyme are designed, digestibility of vinegar residues is increased, the function of anti-nutrition is reduced while digestible nutrient concentration of vinegar residues is increased, the growth performance is not decreased while costs are reduced when the feed is used, and the scheme of utilizing vinegar residues to reduce cultivation costs in meat duck breeding is feasible. Besides, a proper amount of fibers can promote growth and development of gizzards and intestines of meat ducks, and individually selling duck intestines can further increase economic benefits and economic value added in meat duck breeding.

The invention discloses a mannanase and the coding gene, a recombinant vector and a host cell comprising the gene, and expression of the recombinant vector in E.coli and production of the enzyme in yeast cells by using the recombinant vector. The present invention also provides a new wild type subspecies of Bacillus Megaterium, in particular, Bacillus Megaterium FB101.

The present invention belongs to a kind of medication especially for improving sleep combinated by biological preparation and chinese medicine,the characteristics of which is that it includes Alpha-mannan peptides 20%-40%.melatonin 0.2%-5%.anshenbunao class chinese medicine 55%-75%.that narrate drug includes Alpha-mannan peptides 35%-50%.melatonin 0.8%-3%.anshenbunao class chinese medicine 30%-40%.nourishing spleen-qi heat-clearing and edtoxicating chinese medicine 10%-30%.that narrate drug includes Alpha-mannan peptides 10%-30%.melatonin 0.8%-3%.anshenbunao class chinese medicine 20%-60%.nourishing spleen-qi heat-clearing and detoxifying chinese medicine 10%-30%.blood-nourishing blood-activating and heat-clearing chinese medicine 10%-30%.This medication is synthesized from biological preparation and traditional chinese medicine and combines the modern medical science and traditional scientific ingeniously,the advantage of this medication is that it possesses good efficacy especially to the treatment of sleep disorders and no-toxic side effects,took conveniently.

The invention relates to a compound additive for pigs. Each kilogram of the additive contains 10,000,000 to 1,000,000,000 units of mannase, 100,000 to 800,000 units of pectinase, 6,000,000 to 600,000,000 units of xylanase, 500,000 to 60,000,000 units of cellulase, 10 to 200 grams of oligosaccharide and 10 to 40 grams of acidifying agent and 10 to 100 grams of yucca extract.

The invention discloses panax japonicus polysaccharide.The main chain structure thereof is Beta-D-1 to 3-mannan and/or Alpha-D-3 to 4-mannan and heterosaccharide containing galactan, xylan and glucan, the weight-average molecular weight of the panax japonicus polysaccharide is 5,000 to 2,000,000, the intrinsic viscosity at 25 DEG C is 1 to 100ml/g, and the mole ratio of the three elements of C: H: O is 1: 2: 1. The preparation of the panax japonicus polysaccharide comprises the following steps: after being dried, smashed and skimmed, the panax japonicus is soaked by water under the normal temperature and is stirred for separation, the residue is extracted by high-pressure hot water, further extracted by alkali and reductive salt, and finally extracted by acid, and the panax japonicus polysaccharide which can be dissolved in water and DMSO and not be dissolved in methanol, ethanol and acetone is obtained by separation, neutralization by taking the solution, dehydration, protein removal, decolorization, desalination and drying. The panax japonicus polysaccharide which is prepared by the invention has higher anti-tumor activity and promotion of immune activity, which can be used for preparing drugs or health care products for improving immune function and anti-tumor. The method for preparing the product is simple and easy to operation, and the sources of the needed raw materials are wide.

The invention discloses a micropatterned hydrogel coating, its preparation method and use. The preparation method comprises the following steps: using one of carrageenan, locust bean glue, Arabic gum, xanthan gum, brown algae glue, brown algae sugar glue, xylan, agarose, mannan, galactomannans and glucomannans as a raw material to prepare a hydrogel solution first; spreading the hydrogel solution on a carrier such as a three-dimensional cell ball culture dish or a culture plate and the like; obtaining the coating with lattice patterns uniformly distributed on the surface through moulding and solidification demoulding. The micropatterned hydrogel coating may be used to produce culture products, and the culture products may enable a plurality of cells to propagate in a uniform multicellular ball way without other exogenous power so that the batch preparation of three-dimensional multicellular balls may be realized. The raw materials are easily available, quite low in cost, and natural and toxicity-free; the prepared coating belongs to a transparent nondiffusible coating; the growth of the cells in an animal body is highly simulated; and the coating has excellent stability and biological activity and is applicable to most cell culture products.

The invention discloses high-protein fermented soybean meal and a preparation method of the high-protein fermented soybean meal, and belongs to the field of animal feeds. The preparation method of the high-protein fermented soybean meal comprises the following steps: grinding soybean meal raw materials, adding water to carry out sterilization, adding a compound enzyme preparation to carry out enzymatic hydrolysis, after the enzymatic hydrolysis is carried out for a period of time, adding activated lactic acid bacteria or yeast simultaneously, performing semi-solid state fermentation, and then solid-liquid separation, drying and grinding to obtain the high-protein fermented soybean meal; the compound enzyme preparation contains alpha-galactosidase, cellulase, xylanase, pectinase, beta-mannase and beta-glucanase. According to the invention, through the specific composition and addition amount of the compound enzyme preparation, the synergism among various enzymes and a method of combining adding of the compound enzyme preparation with mixed fermentation, the content of non-starch polysaccharides can be reduced to 8% to 10%, the protein content reaches up to 60.2%, essential amino acids are overall improved by 22.41%, other various indexes obviously become better, and the method disclosed by the invention plays an important role in improving the quality of the soybean meal.

The invention discloses beta-mannanase and a preparation method thereof. The beta-mannanase provided in the invention is a protein which is a protein 1, a protein 2 or a protein 3, wherein the protein 1 is composed of a 22-416 amino acid sequence shown in a sequence 1 in a sequence table; the protein 2 is composed of an amino acid sequence represented by the sequence 1 in the sequence table; and the protein 3 is obtained through substituting and/or deleting and/or adding one or more amino acid residues to an amino acid residue sequence of the protein 1 or the protein 2, has activities of the beta-mannanase, and is derived from the protein 1 or the protein 2. Engineering bacteria formed by introducing protein coding gen into Pichia pastoris are fermented in a 5L fermenting tank in a high density manner, and the enzymatic activity of a fermenting solution can reach 50029.6U/mL (the protein content is 6.1mg/mL), so efficient express is realized. The beta-mannanase of the invention has application potentials in the food industry, the medicine industry, the papermaking industry, the forage industry, the petroleum exploitation industry, the fine chemical engineering industry and the like.

A manna peptide injection for intravenous drip is prepared from manna peptide, isotonic substance and water through preparing an aqueous solution of manna peptide, mixing it with an aqueous solution of isotonic substance, loading in bottle, sealing and sterilizing. The application method of this injection is also disclosed.

The invention discloses an acid-resistant and high temperature-resistant beta-mannase gene and application thereof. The beta-mannase gene has the nucleotide sequence shown as SEQ ID No. 1 as well as the amino acid sequence shown as SEQ ID No. 2. The invention also discloses the application of the acid-resistant and high temperature-resistant beta-mannase gene to the preparation of an acid-resistant and high temperature-resistant beta-mannase. The optimum pH value and the optimum temperature of the beta-mannase are 4.6 and 70 DEG C respectively; the beta-mannase has certain characteristics of acid resistance and high temperature resistance, and is particularly applied to industries, such as foods, feeds, paper making, printing and dyeing, and textile.

The invention provides a suit finishing agent and a preparation method thereof. The suit finishing agent comprises the following components in parts by weight: 4-6 parts of ethoxy aminopropyl dimethicone, 2-4 parts of ammonium dihydrogen phosphate, 3-5 parts of calcium hydrophosphate, 7-9 parts of sodium carboxymethylcellulose, 5-6 parts of mannan, 3-5 parts of octylphenol polyoxyethylene, 35-55 parts of ethanol and 210-220 parts of water. The suit finishing agent prepared through the steps of heating the components of the finishing agent after adjusting the temperature, agitating the components with an agitator, filling nitrogen and the like has better water and oil repellent and anti-wrinkle effects. A set of suit has water and oil repellent and anti-wrinkle performances.

The invention relates to the field of gene engineering, in particular to beta-mannaseBA-Man5A with a wide pH range, a gene thereof and application of the gene. The invention provides the beta-mannaseBA-Man5A with the wide pH range. The beta-mannaseBA-Man5A with the wide pH range has the amino acid sequence shown as SEQ ID NO.1 or 2. The invention also provides a gene for coding the beta-mannaseBA-Man5A with the wide pH range, a recombinant vector and a recombinant strain which comprise the gene, and application of the gene. The nucleotide sequence of the gene is shown as SEQ ID NO. 3 or 4. The beta-mannaseBA-Man5A has high activity in acidic, neutral and alkaline ranges, a wide pH range, high acid and alkali resistance, high heat resistance and excellent anti-protease ability, and can be applied to industries such as animal and fish feed, food, medicines, paper making and the like. According to the technical scheme of the invention, mannase can be produced by a gene engineering method.