67 results about "Mannoglucan" patented technology

The invention relates to the technical field of functional food, in particular to immune regulation type prebiotic syrup with synergy of galactomannan. The active components of the composite solid beverage are prepared from the following raw materials in percentage by weight: 20%-35% of fructo-oligosaccharide, 10%-18% of galactooligosaccharide, 8%-15% of stachyose, 3%-7% of xylooligosaccharide, 5%-12% of lactitol, 5%-10% of galactomannan, 2%-5% of yeast beta-glucan and 1%-3% of N-acetylglucosamine. The composition further comprises 4-8% of inulin and 3-6% of resistant dextrin as a matrix filling and slow-release carrier, and the sum of the weight percentages of all the active ingredients and the carrier is 100%. By remodeling intestinal microecology and inhibiting inflammation pathways, the intestinal inflammation state can be remarkably improved, expression of intestinal mucosa tight junction protein is promoted, and an innovative solution is provided for people suffering from chronic bowel diseases and immune imbalance.

The invention belongs to the technical field of food processing, and particularly relates to a product rich in soluble yeast dietary fibers and a preparation method and application thereof. Wherein in terms of dry matter mass of the product containing the soluble yeast dietary fiber, the product containing the soluble yeast dietary fiber comprises the following components in percentage by mass: more than 40% of soluble yeast dietary fiber, less than 0.5% of fat, more than 20% of yeast beta-glucan and more than 20% of mannan. The product containing the soluble yeast dietary fibers prepared by the preparation method disclosed by the invention contains the soluble dietary fibers with higher content, the solubility is obviously improved, the mouth feel is improved to a certain extent, and the product contains lower fat content and is more beneficial to storage; and meanwhile, the product is rich in beta-glucan and mannan, so that the product has a wide application prospect.

To provide konjak-like food with high protein content, while utilizing the advantages of konjac, which has a unique texture and a low caloric value.SOLUTION: A liquid mixture contains 0.3 w / w% or more and 2.5 w / w% or less of glucomannan, 0.2 w / w% or more and 2.5 w / w% or less of alginic acid and / or a salt thereof, and 3.0 w / w% or more and 12.0 w / w% or less of vegetable protein, optionally containing 0.1 w / w% or more and 1.5 w / w% or less of xanthan gum, and substantially free of gluten. The liquid mixture is discharged into an aqueous solution containing divalent cations to produce a gel-like product.SELECTED DRAWING: None

The application discloses a high-stability immobilized mannanase and a preparation method and application thereof, and belongs to the technical field of enzyme immobilization. The preparation method uses PAMAM modified SBA-15 / calcium alginate composite microspheres as a carrier to fix mannanase in an enzyme solution by adopting an embedding-covalent crosslinking method, so as to obtain the high-stability immobilized mannanase. The preparation method uses the PAMAM modified SBA-15 / calcium alginate composite microspheres as the carrier to fix the mannanase by adopting the embedding-covalent crosslinking synergistic method, so that the pH tolerance, the circulation stability and the hypoglycemic efficiency of the enzyme are improved, and the problems of the traditional immobilized enzyme, such as large enzyme activity loss, easy leakage and poor stability under high temperature, are solved.

[Problem] To provide a composition for regulating blood pressure for a healthy person. [Solution] A blood-pressure-regulating composition for a healthy person, said composition being characterized by containing a guar gum degradation product, and being characterized in that: 70 mass% or more of the guar gum degradation product has an average molecular weight within the average molecular weight range of 1.0 × 103 to 1.0 × 105; the composition has a viscosity of 10 mPa·s or less when a 2 mass% aqueous solution at 37°C is measured at 10 rpm using an E-type viscometer, or has a viscosity of 5 mPa·s or less when the aqueous solution is measured at 20 rpm, or has a viscosity of 3 mPa·s or less when the aqueous solution is measured at 30 rpm; the composition is obtained by hydrolyzing a galactomannan polysaccharide which contains guar-derived endosperm and in which the content ratio (galactose:mannose) of galactose and mannose is within the range of 1:1.3 to 1:2, by using a microorganism-derived β-mannanase to reduce the molecular weight of the polysaccharide; and the composition contains at least 70 mass% of dietary fiber in terms of the dietary fiber content stipulated in the enzyme-HPLC method.

The invention relates to a β-glucan-rich Saccharomyces cerevisiae yeast cell wall extract for use in the prevention or treatment of a disease associated with or caused by a Lawsonia intracellularis infection in pigs, characterised in that the extract comprises β-glucans and mannans, where the β-glucans are present in the form of a mixture of β-1,3-glucans and β-1,6-glucans in a total amount of greater than or equal to 50% by weight, notably of between 50% and 99% by weight, and where the mannans are present in a total amount less than 5% by weight, notably of between 1% and 5% by weight.

The invention discloses highly acetylated glucomannan and application thereof in preparation of a product with an effect of relieving prediabetes mellitus, and belongs to the technical field of medicines. In-vivo experiments prove that the glucomannan with high acetylation degree has the effect of relieving the prediabetic stage, which is specifically reflected in weight gain of mice in the prediabetic stage, regulation of blood glucose metabolism, improvement of glucose tolerance and insulin resistance, regulation of blood lipid metabolism, protection of liver and pancreatic tissues and reduction of epididymal fat cell size. The invention provides an important basis for developing a dietary formula suitable for prediabetic patients, and provides a new method for prevention and management of diabetes mellitus.

The present application relates to the technical field of genetic engineering and protein engineering, and particularly provides a mannanase mutant and application thereof.The mutant is obtained by mutating the 175th amino acid of mannanase with the amino acid sequence of SEQ ID NO:4 from Ser to Thr, and the specific activity of the mutant is significantly higher than that of the wild type, so that the mutant can be widely applied to the production of livestock and poultry feed and marine aquaculture feed.

This invention discloses a composition that can effectively soothe and repair sensitive skin, its preparation method, and its application, relating to the field of skincare products. The composition of this invention, by weight, comprises 5-18 parts β-glucan, 1-10 parts mannan, 8-15 parts yeast polypeptides, 1-10 parts sodium hyaluronate, 2-5 parts magnesium ascorbate phosphate, and 8-15 parts Scutellaria baicalensis root extract. This composition is mild and non-irritating, can quickly and effectively relieve skin sensitivity reactions, protect the skin barrier, and has good storage stability, not easily affected by environmental factors such as temperature and oxygen.

A mannanase at least 75% identical to SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4, a poly-nucleotide encoding mannanase, an expression construct comprising the polynucleotide, and a host cell comprising the polynucleotide or the expression construct.

The present invention relates to newly idenified GH26 mannanases and their use as enhancers of enzyme benefits in detergents as well as a detergent composition comprising said mannanases.

The present invention provides a functional paper product which has water resistance, oil resistance and can be recycled. A functional paper product (1) according to the present invention comprises a base paper (20) and a coating film (10) formed on the base paper (20), the coating film (10) having, as a main component, modified mannan in which acetyl groups in konjac mannan are free, the coating film (10) being obtained by curing a swollen modified mannan sol in a state in which the swollen modified mannan sol is immersed in gaps between cellulose fibers of the base paper (20), and the viscosity of the swollen modified mannan before curing is 3000 mPa * s or less.

The present application provides a soluble dietary fiber composition, its preparation method and application. After the soluble dietary fiber composition enters the colon, the ester bond in the alanine modified pectin is rapidly degraded, part of the chlorogenic acid is released, the secretion of anorectic hormone is stimulated, the appetite is reduced, and the heat intake is reduced; the remaining amide bond network is slowly degraded, realizing the slow and continuous release of chlorogenic acid. Lutein removes free radicals generated by fat decomposition, reducing the oxidative damage of L cells. Galactomannan and alanine modified pectin-glucan modified protein-chlorogenic acid ternary complex synergize to improve the molar ratio of butyric acid / propionic acid: propionic acid enhances satiety through intestinal gluconeogenesis, and butyric acid stimulates the secretion of GLP-1 / PYY, realizing endogenous appetite suppression. Galactomannan absorbs water to form a lubricating gel, softening the stool and increasing the stool volume; the ternary complex degrades to produce short-chain fatty acids, optimizing the intestinal microenvironment and enhancing gastrointestinal peristalsis, both of which synergistically improve constipation.

This application discloses a muscle stem cell resuscitation and protection washing solution, a quality assessment kit, and a method of use, belonging to the field of cell biology technology. In this application, vitamin E succinate and mannan peptide are used as core antioxidant components, synergistically combined with Gynostemma pentaphyllum polysaccharide, Portulaca oleracea polysaccharide, trehalose, L-proline, and magnesium sulfate heptahydrate to construct a four-in-one resuscitation and protection washing solution that provides isotonic buffering, extracellular protection, intracellular protein stabilization, antioxidant effects, and mitochondrial stabilization. This increases the survival rate of muscle stem cells after resuscitation to over 93%. The kit integrates a multi-dimensional detection system for cell resuscitation and protection washing solution, cell viability, proliferation rate, Pax7 / MyoD double-positive stemness assessment, and cryopreservation damage, achieving closed-loop management of the entire process of muscle stem cell resuscitation, protection, and quality assessment. The double-positive rate of stemness markers reaches over 90%, providing reliable quality assurance for cell bank management and clinical applications.

The application discloses a kind of feed additive and its application, it is related to feed additive technical field.The prepared feed additive of the application is by mixing sodium glucuronate, glucomannan, dimethyl cyclodextrin, diatomite and sodium lauryl sulfate, then mixed solution is reacted with calcium chloride crosslinking solution, finally form microspheres type feed additive.Compared with prior art, the prepared feed additive of the application is beneficial to the adsorption of deoxynivalenol and zearalenone, amino acid liquid, lemon yellow and titanium dioxide are mixed, then composite bacteria liquid is added to ferment, by feed additive processing, low-temperature drying can retain the nutrient components and biological activity in raw materials, and the biological fermentation protein raw material with good sensory color is obtained.

The present invention aims to provide an intraoral adhesive film that combines adhesive properties and slow dissolution properties in a well-balanced manner. [Solution] The present invention provides an intraoral adhesive film containing (A) ιcarrageenan and (B) κcarrageenan. Preferably the film further contains (C) a water-soluble polymer such as tamarind gum, gum arabic, karaya gum, xanthan gum, gellan gum, tragacanth gum, guar gum, tara gum, locust bean gum, pullulan, gelatin, casein, pectin, agar, glucomannan, galactomannan, starch, carboxymethyl starch, dextrin, alginic acid, alginic acid ester, chitin, chitosan, carboxymethylcellulose, λcarrageenan, hydroxyethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, methylcellulose, and ethylcellulose.

An aqueous cosmetic composition for keratin fibers comprising: a) at least one acetylated or non-acetylated glucomannan polymer, b) at least one linear or branched, saturated or unsaturated C12-C22 fatty alcohol, c) at least one conditioning agent.

The invention belongs to the field of food, and particularly relates to a yeast enzymatic hydrolysate, a yeast protein and a yeast protein sodium salt product with high emulsifying performance as well as preparation and application of the yeast enzymatic hydrolysate and the yeast protein sodium salt product. The emulsifying activity of the yeast enzymatic hydrolysate is greater than or equal to 25.0 m / g, and the emulsifying stability is greater than or equal to 77.0%; the emulsifying activity of the yeast protein is greater than or equal to 70.0 m / g, and the emulsifying stability is greater than or equal to 175.0%; the emulsifying activity of the yeast protein is greater than or equal to 70.0 m / g, and the emulsifying stability is greater than or equal to 175.0%. According to the preparation method disclosed by the invention, the yeast is subjected to composite enzymolysis through the alkaline protease, the flavourzyme, the glucanase, the mannase and the glutamine transaminase and then is subjected to ionization reaction with the sodium hydroxide to obtain the yeast protein sodium salt product, and the yeast protein sodium salt product has relatively high emulsifying activity and also has relatively good color and flavor; the sodium caseinate can be used as a natural emulsifier, and the problems of limited sodium caseinate yield, unstable supply and high price are solved.

The invention relates to the field of traditional Chinese medicines, and discloses radix tetrastigme polysaccharide, a preparation method thereof and application of the radix tetrastigme polysaccharide in preparation of antithrombotic drugs. The novel acid glucuronic acid galactomannan (THP5) is extracted from the overground part of the radix tetrastigme polysaccharide for the first time, it is found that the novel acid glucuronic acid galactomannan (THP5) can be used for preventing and treating thrombus diseases including arterial thrombosis, venous thrombosis and the like in a multi-target mode, the application range of the radix tetrastigme polysaccharide is widened, and a new way is provided for treating the thrombus diseases.

The purpose of the present invention is to provide an intraoral adhesive film which has a good balance of adhesive properties and sustained-release properties. Specifically, the present invention provides an intraoral adhesive film containing (A) ι carrageenan and (B) κ carrageenan. It is preferable that the film further contain (C) a water soluble polymer such as tamarind gum, gum arabic, karaya gum, xanthan gum, gellan gum, tragacanth gum, guar gum, tara gum, locust bean gum, pullulan, gelatin, casein, pectin, agar, glucomannan, galactomannan, starch, carboxymethyl starch, dextrin, alginic acid, alginic acid ester, chitin, chitosan, carboxymethyl cellulose, λ carrageenan, hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, methyl cellulose, or ethyl cellulose.

The invention relates to the technical field of oil and gas development materials, and particularly discloses a gel breaking temporary plugging agent and a preparation method thereof. The gel breaking temporary plugging agent is prepared from the following raw material components: beta-mannase, a calcium salt solution, ammonium persulfate microcapsules, oxidation modified bacterial powder, silicon dioxide microspheres, beta-mannan-based gel and water. The gel breaking temporary plugging agent provided by the invention effectively solves the problems of incomplete gel breaking, easy generation of secondary plugging and poor controllability of gel breaking time of a traditional temporary plugging agent.

The invention relates to the field of breeding and food, and discloses a fattening pig feed, a fattening pig feeding method and a low-cholesterol pigtails, the feed comprises 5-35 wt% of citrus residue fermentation product, 50-70 wt% of corn and 10-25 wt% of soybean meal based on the total amount of the fattening pig feed; wherein the fermented citrus pulp comprises citrus pulp, auxiliary materials, microbial agents and enzyme preparations, the auxiliary materials comprise corn and soybean meal, the microbial agents comprise bacillus subtilis and bacillus licheniformis, and the enzyme preparations comprise xylanase, glucanase, mannase and cellulase. After fattening pigs are fed with the feed provided by the invention, the obtained pigtails have the characteristics of low fishy smell, good smell, low cholesterol content, high calcium and magnesium content and the like. The operation method provided by the invention is simple and easy to implement, and the quality improvement effect of the pigtail food material is remarkable.

In the present invention new enzyme compositions comprising a mannanase are provided. More specifically, the present invention provides enzyme compositions comprising a mannanase and at least one additional enzyme selected from the group consisting of amylase and protease and methods of making and using these enzyme compositions and methods of washing with these enzyme compositions.

This invention relates to a Bacillus subtilis ( Bacillus subtle Y32, with accession number CGMCC No. 34819, is a strain that produces heat-resistant and highly stable xylanase, as well as various hydrolases such as β-mannanase and protease. It also produces extracellular polysaccharides, inhibits Botrytis cinerea, and is acid-resistant, resistant to porcine bile salts, and resistant to 10% NaCl. The optimal temperature for the xylanase produced by this strain is 55℃, and the optimal pH is 6.0. Under these conditions, the half-life is 12.5 h, and the relative enzyme activity remains above 80% after 3 h of incubation, demonstrating good thermal stability. This strain can also increase the yield of cucumbers and Pinellia ternata, and increase the egg production rate of 70-week-old Hy-Line Brown hens from 70.4% to 90.1%. This strain has promising applications in agriculture, food processing, and cosmetics production.

The invention discloses a preparation method and application of whole-protein woven network hydrogel, and belongs to the technical field of biological materials. According to the preparation method disclosed by the invention, by designing a pseudo-cable hydrocarbon dimer (BXA ') and utilizing stimulation factors such as concentration, calmodulin (CaM), photoresponse CaM (hv), temperature and / or light to induce topological conversion of the pseudo-cable hydrocarbon dimer into a star-shaped molecule, intermolecular crosslinking is realized, and then spontaneous polymerization is performed to form a whole-protein woven network, so that the whole-protein woven network has dynamically adjustable mechanical properties; the ultrahigh-modulus full-protein woven network hydrogel with multiple stimulation responsiveness is realized. Further, the hydrogel can be used for immobilization and controllable release of enzymes (such as mannase). The whole-protein woven network hydrogel provided by the invention has the advantages of high biocompatibility, controllable structure, diversified response mechanisms and the like, and has wide application prospects in the fields of biomedicine, intelligent drug loading, tissue engineering and the like.

The invention relates to the technical field of molecular biology and biological medicine, in particular to a siTGF-beta1 / siGAS6 co-delivery system capable of being used for aerosol inhalation synergistic treatment of idiopathic pulmonary fibrosis. Specific siTGF-beta1 is designed aiming at mRNA (messenger ribonucleic acid) of a transforming growth factor TGF-beta1, and the siTGF-beta1, siGAS6 capable of effectively inhibiting GAS6 gene expression and a mannan-polypeptide conjugate carrier are further compounded to form a mannan-polypeptide conjugate co-delivery system of the siTGF-beta1 and the siGAS6, namely a mannan-polypeptide / siTGF-beta1 / siGAS6 co-delivery system. The system can be used for preparing a medicine for treating idiopathic pulmonary fibrosis, especially for preparing a medicine capable of being inhaled by atomization. The idiopathic pulmonary fibrosis can be synergistically treated in an aerosol inhalation mode. According to the technical scheme, lung macrophages can be efficiently targeted, and a synergistic treatment effect is generated to realize efficient treatment on idiopathic pulmonary fibrosis.

A mannanase at least 85% identical to SEQ ID NO: 1, a polynucleotide encoding the mannanase, an expression construct comprising the polynucleotide, and a host cell comprising the polynucleotide or the expression construct.

The invention discloses a preparation method and application of beta-glucan, and belongs to the technical field of bioengineering.The preparation method comprises the steps that saccharomyces cerevisiae genetically engineered bacteria producing beta-glucan are treated in the mode that autolysis and high-temperature extraction are combined, mannan in yeast cell walls is removed, then specific ultrasonic treatment and impurity removal are conducted, and the beta-glucan is obtained. And the beta-glucan is prepared at a high extraction rate. After beta-glucan is prepared, carboxymethyl glucan prepared through carboxymethylation modification can be used for repairing and protecting ultraviolet radiation skin light aging, and is used for skin oxidation resistance, inflammation inhibition and skin collagen stability maintenance. The method is used for efficiently preparing the beta-glucan.

To provide a composition capable of providing good makeup removability and a peculiar feeling of use.SOLUTION: A composition, preferably a cosmetic composition, more preferably a cosmetic composition for cleansing, comprising (a) at least one glucomannan and (b) at least one oil, wherein the amount of (a) glucomannan in the composition is 0.5% by weight or more, relative to the total weight of the composition.SELECTED DRAWING: None

The application belongs to the field of food, and particularly relates to a yeast enzymatic hydrolysate with high emulsification performance, a yeast protein, a yeast protein sodium salt product, and preparation and application thereof. The emulsification activity of the yeast enzymatic hydrolysate is greater than or equal to 25.0 m2 / g, and the emulsification stability is greater than or equal to 77.0%; the emulsification activity of the yeast protein is greater than or equal to 70.0 m2 / g, and the emulsification stability is greater than or equal to 175.0%; the emulsification activity of the yeast protein is greater than or equal to 70.0 m2 / g, and the emulsification stability is greater than or equal to 175.0%. In the application, the yeast is subjected to complex enzymolysis by alkaline protease, flavour protease, glucanase, mannanase and glutamine transaminase, and then subjected to ionization reaction with sodium hydroxide to obtain a yeast protein sodium salt product. The yeast protein sodium salt product has high emulsification activity, good color and flavor, and can be used as a natural emulsifier, thereby solving the problems of limited production of sodium caseinate, unstable supply and high price.