Production method for preparing high-purity oligosaccharides by hemicellulose enzymolysis

A mannan oligosaccharide and hemicellulose technology, applied in the field of enzyme technology and biochemical industry, can solve the problems of low substrate concentration, low product purity and high cost, and achieve no chemical pollution, high product purity and controllable cost Effect

Inactive Publication Date: 2012-03-14
深圳绿天琪生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, in the research on the preparation method of low-carbon chain mannose heteropolymers, either the enzyme activity is not high, the substrate concentration is low, the cost is high, and the basis for industrial production is lacking; or the addition of alkali and acid during the hydrolysis process , which brings difficulties to the post-treatment process and is not conducive to environmental protection; or the purity of the product is low, generally only 70-80%, although in the research results of Huang Daiyong et al. (2007), the purity of konjac

Method used

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  • Production method for preparing high-purity oligosaccharides by hemicellulose enzymolysis
  • Production method for preparing high-purity oligosaccharides by hemicellulose enzymolysis
  • Production method for preparing high-purity oligosaccharides by hemicellulose enzymolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] l. Enzyme hydrolysis;

[0021] a) Add water: In a 1000L enzymatic hydrolysis tank, based on an effective capacity of 700kg, konjac gum: water = 20:80 (w / w), add 560kg of water to the degradation tank, and the pH of the water is between 6.5 and 7.5.

[0022] b) Preheating: Steam or hot water is introduced into the jacket of the enzymatic hydrolysis tank to preheat the water in the tank to 50-52°C.

[0023] c) Enzyme addition: After the degradation water temperature is stable, add β-mannanase BM-II according to the ratio of enzyme to substrate of 1: 20-30, and keep stirring to make it evenly mixed, and keep it warm for 5-10 minutes. Feeding.

[0024] d) Feeding: Weigh 140kg of konjac gum and add it to the enzymatic hydrolysis tank with a substrate concentration of 15-20%, and stir well.

[0025] e) Enzymolysis: Keep the temperature in the tank between 50-55°C, stir constantly, and perform enzymolysis for 3-4 hours. In the process of enzymolysis, use NDJ-1 rotary viscom...

Embodiment 2

[0033] l. Enzyme hydrolysis;

[0034] a) Add water: In a 1000L enzymolysis tank, based on the effective capacity of 700kg, guar gum: water = 15:85 (w / w), add 595kg of water to the enzymolysis tank, and the pH of the water is between 6.5 and 7.5 .

[0035] b) Preheating: Steam or hot water is introduced into the jacket of the enzymatic hydrolysis tank to preheat the water in the tank to 50-52°C.

[0036] c) Enzyme addition: when the temperature is stabilized at the required temperature, add β-mannanase BM-II according to the ratio of enzyme to substrate of 1: 10-15. Stir continuously to make it evenly mixed, and keep warm for 5-10 minutes before feeding.

[0037] d) Feeding: Weigh 105kg of guar gum and add it into the enzymatic hydrolysis tank (substrate concentration 15%), and stir well.

[0038] e) Enzymolysis: Keep the temperature in the tank between 50-55°C, stir constantly, and perform enzymolysis for 3-4 hours. During the enzymolysis process, use NDJ-1 rotary viscomet...

Embodiment 3

[0042] The method of preparing P98 mannooligosaccharides using locust bean gum, quince gum, acacia gum, and fenugreek gum as raw materials and using β-mannanase BM-II is the same as in Example 2.

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Abstract

The invention provides a method for preparing high-purity oligosaccharides by the enzymolysis of hemicellulose containing mannosan, and a product which is a high-effect beta-mannase BM-II produced by fermenting bacillus subtilis TQBm with the preservation No. of CCTCC No: M 211147. Konjac glucomannan, guar gum, sophora bean gum, sesbania gum and other hemicelluloses containing the mannosan are hydrolyzed into relatively small molecule materials, and then are desalted, decolored and deodorized through centrifugal separation, flocculation clarification and ion exchange techniques, large molecule materials in sugar solution are separated by the combination of an ultrafiltration technique and a molecular sieve chromatography technique, and finally the high-purity oligosaccharides is prepared through concentration and drying. The method for preparing the high-purity oligosaccharides disclosed by the invention has the advantages of simple operation, good separation effect, low cost and no chemical pollution, and industrialized production can be realized. The oligosaccharides product disclosed by the invention can obviously proliferate bifidobacteria in the human intestinal tract and otherbeneficial bacteria and obviously reduce enterobacter and other harmful bacteria, has the functions of blood sugar and blood fat reduction and immunoregulation, and is a novel raw material and assistant for gastrointestinal health, blood sugar and fat reducing, immunoregulation and disease resisting and preventing medicines.

Description

technical field [0001] The invention relates to enzyme technology and biochemical technology, more precisely, it is a preparation method and product of mannan oligosaccharides with a purity of 98% (P98) by enzymatically hydrolyzing hemicellulose containing mannan. , Immunomodulation, new raw materials and additives for anti-disease and disease-preventive drugs. Background technique [0002] Hemicellulose refers to the part of plant polysaccharides that grow symbiotically with cellulose in plant cell walls, are soluble in alkaline solutions, and are far easier to hydrolyze than cellulose when exposed to acid. There are three main types of hemicelluloses, namely xylosaccharides, polyglucomannose and polygalactosylglucomannose. The hemicellulose involved in the present invention is polyglucomannose (representative product is konjac gum) and polygalactomannose (representative product is guar gum), collectively referred to as mannan. [0003] Mannan in hemicellulose is enzymati...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 黄代勇钱金宏汪修武李小虎廖园园黄伟刘仁武刘雯
Owner 深圳绿天琪生物医药有限公司
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