70results about How to "High product purity" patented technology

The invention belongs to the field of gene engineering. Specifically, the invention relates to a system, a tool and a method for modifying or editing target nucleotide bases, and more specifically, the invention relates to an optimized QBE base editing system and application thereof. The base editing system disclosed by the invention has the advantages of high efficiency of a gene editing tool, high product purity, low Indel frequency, low off-target frequency and abundant gene editing windows, and has important value for expanding application scenes of a base editing technology in the fields of life science basic research, agriculture, biological medicine and the like.

The invention discloses a solvent-free preparation method of 2-thiophenesulfonyl fluoride, which comprises the following steps: carrying out nucleophilic fluorination reaction on 2-thiophenesulfonyl chloride under the action of a fluorination reagent at 0-70 DEG C to generate a 2-thiophenesulfonyl fluoride crude product, distilling under a vacuum condition, and separating the product at 30-100 DEG C to obtain colorless and transparent high-purity 2-thiophenesulfonyl fluoride. The method provided by the invention has the advantages of easily available raw materials, simple synthesis process, high product purity, high yield, large material contact area by adopting a solvent-free method, and reaction time saving; the method does not need solvent recycling and post-treatment links, is energy-saving and environment-friendly, saves the production cost, achieves the purpose of clean production, and has a wide application prospect.

The utility model discloses a device for preparing glyoxylic acid, which comprises the following components: a reaction kettle which is provided with a raw material inlet, an ozone inlet, a reactant outlet, a foam outlet and a liquid return port on the kettle wall and is internally provided with an aeration disc, a stirring mechanism and a foam absorption mechanism; a foam inlet, a liquid outlet, a tank body cooling liquid inlet and a tank body cooling liquid outlet are formed in the tank wall of the foam treatment tank, the foam inlet is connected with the foam outlet, and the liquid outlet is connected with the liquid return opening; a defoaming mechanism and a first heat exchange coil are arranged in the tank, one end of the first heat exchange coil is connected with the tank body cooling liquid inlet, and the other end of the first heat exchange coil is connected with the tank body cooling liquid outlet; the pump body is respectively connected with the reaction kettle and the foam treatment tank; and the cooling liquid circulating treatment mechanism is respectively connected with the tank body cooling liquid inlet and the tank body cooling liquid outlet. According to the utility model, foams generated in the process of synthesizing glyoxylic acid through oxidation reaction can be effectively recovered and utilized, and the product preparation efficiency and the product purity are improved.

The utility model relates to a kind of for prothioconazole oxidation synthesis light heavy component automatic separation device, belong to chemical production technical field, including prothioconazole oxidation synthesis component phase tank, mixed liquor regulating valve, light component regulating valve, heavy component regulating valve, flowmeter, mixed liquor regulating valve, light component regulating valve, heavy component regulating valve, flowmeter are all set to prothioconazole oxidation synthesis component phase tank, prothioconazole oxidation synthesis component phase tank is provided with mixed liquor feed inlet, light phase outlet, heavy phase outlet, blow-off, the utility model, optimization tank body structure realizes unpowered high-efficiency separation, reduce equipment cost, improve separation precision and product purity, by phase tank stratification structure optimization, light / heavy phase outlet height difference design and valve control, to effectively reduce entrainment rate, interface height and flow are monitored in real time, dynamically regulate valve opening, when heavy component proportion increases, automatically increase heavy component valve opening, to effectively reduce artificial dependence.

The application discloses a production device and method for enhancing carbon dioxide production, belonging to the technical field of carbon dioxide preparation, which comprises a buffer tank, a compression device, a desulfurization device, a refining device, a liquefaction device, a purification device, a storage bottle, pipeline one, pipeline two, pipeline three, pipeline four, pipeline five, pipeline six, the buffer tank is connected with the compression device through the pipeline one, the desulfurization device is connected with the compression device through the pipeline two, the refining device is connected with the desulfurization device through the pipeline three, the liquefaction device is connected with the refining device through the pipeline four, the purification device is connected with the liquefaction device through the pipeline five, and the purification device is connected with the storage bottle through the pipeline six; the produced liquid carbon dioxide has a purity of 99.9%, and the production has high purity and high efficiency.

The utility model discloses a kind of rare earth polishing liquid extraction equipment, it is related to rare earth polishing liquid extraction technical field.The utility model includes jar body, extraction liquid automatic recovery component and fast mixing component, the left side of the jar body is connected with peep window, the bottom of the jar body is fixedly connected with support plate, the extraction liquid automatic recovery component includes control mechanism.The utility model passes through extraction liquid automatic recovery component, using servo motor drive screw rotation, makes it drive screw thread cover, movable frame and industrial camera accurate height adjustment, until industrial camera detects clear demarcation and automatically stops moving, in this process, movable frame drives adjusting rod and telescopic tube synchronous movement, so that collection cover is accurately located in extraction liquid layer, realize extraction liquid automatic recovery, effectively eliminate artificial visual misjudgment and operation lag problem, improve operation continuity and automation level, avoid different density liquid cross-contamination risk, improve extraction efficiency and product purity.

The utility model relates to the field of light curing material processing technology discloses a kind of filtering devices of light curing high viscosity monomer, including support base, the top of support base is provided with support plate, the left side of the top of support base is provided with spiral pressurizing mechanism, the spiral pressurizing mechanism is used to spiral pressure filtration high viscosity monomer to improve filtering effect, the right side of the top of support base is provided with collection mechanism, the top of support plate is provided with filtering mechanism, the right side of filtering mechanism is provided with drainage mechanism, the top of filtering mechanism is provided with temperature control heating mechanism. In the utility model, set up spiral pressurizing mechanism, motor drives screw driving rod, screw pressure plate is axially compact to high viscosity material, left and right side ascending assembly provides orientation, solve the problem that high viscosity material is difficult to filter due to poor flowability, improve the compacting effect of light curing high viscosity monomer, improve filtering efficiency and product purity.

The invention discloses a preparation method of a silybin meglumine tablet, which comprises the following steps: reacting silybin with meglumine under optimized conditions to generate a liquid reactant, omitting a drying link, and directly adding the liquid reactant into an auxiliary material through spray gun atomization for one-step granulation; the auxiliary materials are screened and premixed, and after liquid reactants and the auxiliary materials are uniformly mixed, the temperature and spraying parameters are controlled to complete particle preparation. And after granulation, drying to a target water content by adopting fluidized bed drying equipment, and carrying out particle mixing, tabletting and coating to finally finish aluminum-plastic subpackaging and outer packaging. The process omits the traditional steps of drying, crushing and granulating, prevents reactants from being exposed to a high-temperature environment for a long time, and effectively improves the utilization rate of raw materials, the content of effective components and the purity of the product. Meanwhile, dust is reduced, the production environment is optimized, and the operation safety and the product yield are remarkably improved.

The invention discloses a modified fiber fabric and a preparation method thereof, belongs to the technical field of cellulose, and solves the technical problem that in the prior art, sodium and potassium in a CMC-H modified fiber product remain on a water absorbing material, so that the biocompatibility of the water absorbing material is influenced. According to the modified fiber fabric and the preparation method thereof provided by the invention, the fiber fabric raw material is subjected to modification treatment to obtain carboxymethyl cellulose and ammonia ammoniation treatment in sequence on the basis of keeping the fiber shape, so as to obtain the fiber fabric of which the modifier is the ammonium carboxymethyl cellulose; the fiber fabric raw material is used for maintaining a fibrous modification treatment reaction, specifically, in the modification treatment reaction process, in fiber molecules of the fiber fabric raw material, 1, 4-glucosidic bonds in glucose molecules are connected to form cellulose chains arranged in parallel, the cellulose chains are not changed, and specific chemical groups on specific carbon positions C6, C2 and C3 on the molecular chains of fibers are subjected to a modification reaction; and the softness and physical properties of the product paper or non-woven fabric are ensured.

The invention belongs to the technical field of fine chemical engineering, and particularly relates to a process for preparing nano calcium carbonate by a wet method. A process for preparing nano calcium carbonate by a wet method comprises the following steps: polymerizing N-isopropylacrylamide and vinyl imidazole to generate a copolymer, then adding the copolymer into a calcium hydroxide suspension to form a crystal nucleus, dispersing the crystal nucleus, introducing carbon dioxide gas to carry out carbonization reaction, introducing nitrogen after the reaction is completed, and carrying out vacuum drying to obtain the nano calcium carbonate. Recovering the copolymer to be neutral and desorbing from the crystal surface; and finally, carrying out centrifugal separation, collecting a solid product, and carrying out vacuum drying to obtain the nano calcium carbonate. The nano calcium carbonate prepared by the process is uniform in particle size and good in dispersity. No toxic or harmful auxiliary agent needs to be introduced in the nano calcium carbonate production process, and the production purity is high.

The invention discloses an aminoglycoside 2 '-phosphotransferase mutant and application thereof, and relates to the technical field of biotransformation, a preparation method of the aminoglycoside 2'-phosphotransferase mutant comprises the following steps: S1, cloning and expressing aminoglycoside 2 '-phosphotransferase; s2, purification of aminoglycoside 2 '-phosphotransferase; s3, determining the enzyme activity of aminoglycoside 2 '-phosphotransferase and the specificity of a substrate; s4, constructing a single point mutant; and S5, construction, expression and screening of an ApIf mutation library. The aminoglycoside 2 '-phosphotransferase mutant capable of specifically degrading GTP without degrading UTP is obtained through cloning, expression, purification and screening, and the problems that GTP is difficult to separate and purify in the UTP production process, the GTP impurity content is high in the UTP production process, and the yield and purity of UTP are low can be solved.

The invention provides a preparation method of furosemide, which comprises the following specific steps: (1) adding 2-amino-4-chlorobenzoic acid and 2-chloromethyl furan into a deep eutectic solvent, and carrying out microwave heating reaction to obtain a reaction solution; and (2) sequentially adding chlorosulfonic acid and thionyl chloride into the reaction liquid to react, adding ammonia water, and then performing supercritical CO2 fluid extraction to obtain furosemide. The synthetic method of the furosemide is simple, efficient and low in energy consumption, unnecessary side reactions can be avoided, extra impurities are not generated, post-treatment mother liquor does not exist, the synthesized furosemide is high in yield and purity, and the synthetic method is suitable for large-scale actual production. According to the method, the deep eutectic solvent is selected as a novel solvent, and compared with a single solvent, the deep eutectic solvent can improve the reaction rate and the product yield and can reduce environmental pollution.

The invention provides a refining method of 3, 3 ', 4, 4'-biphenyltetracarboxylic acid, which comprises the following steps: mixing a 3, 3 ', 4, 4'-biphenyltetracarboxylic acid crude product, acetic acid, a ketone solvent and water at 90-100 DEG C to obtain a biphenyltetracarboxylic acid crude product solution; and mixing the crude biphenyltetracarboxylic acid solution with the seed crystal at 80-90 DEG C, and then carrying out cooling crystallization to obtain a 3, 3 ', 4, 4'-biphenyltetracarboxylic acid refined product. The invention provides a refining method of 3, 3 ', 4, 4'-biphenyltetracarboxylic acid, which has the following advantages: (1) the total metal ions of the product are low and the purity is high, acetic acid is used as an ion exchanger in the refining method, the metal ion removal effect and the product purity can be effectively improved, and the total metal ions can be reduced to 0.2 ppm or below; (2) the yield and the whiteness of the product are high, and the whiteness can be increased to 98% or above; and (3) the refining method is simple and convenient in process.

The invention belongs to the technical field of material synthesis, and particularly relates to calcium dodecahydrododecaborate as well as a synthesis method and application thereof. According to the synthesis method of calcium dodecahydrododecaborate provided by the invention, calcium hydride CaH2, a borane dimethyl sulfide complex and an ether mixed organic solvent react in a reaction kettle to obtain a product, and monoethylene glycol dimethyl ether and tetrahydrofuran cyclic ether are compounded to obtain the ether mixed organic solvent; according to the method, the yield and purity of the product calcium dodecahydrododecaborate are improved, meanwhile, deionized water is used for carrying out solvent exchange on the product and drying is carried out, the purity of the product calcium dodecahydrododecaborate is further improved, efficient synthesis of calcium dodecahydrododecaborate is achieved, industrial preparation is facilitated, high-purity calcium dodecahydrododecaborate is provided, and the method is suitable for industrial production. The method is expected to meet the requirement of solid electrolyte battery commercialization on electrolyte, and solves the technical problem of lack of an efficient synthesis process of calcium dodecahydrododecaborate at present.

The invention belongs to the technical field of drug synthesis, and specifically relates to a method for preparing 4-aminomethylbenzoic acid using urotropine as a catalyst. Para-halogenated methylbenzoic acid is used as a raw material, urotropine and ammonium carbonate are used as catalysts, and reacted with an aminating agent; after the reaction, ammonia is removed by concentration and crystallization to obtain 4-aminomethylbenzoic acid. The 4-aminomethylbenzoic acid prepared by the method has high purity, low cost, less three wastes, is environmentally friendly, and has good industrial production practical value.

The invention provides a method for preparing dimethyl curcumin, comprising steps: 1) acetylacetone and boron oxide react in isopropyl acetate to obtain acetylacetone boron complex; 2) acetylacetone boron complex, veracrucal and tributyl borate react in dimethylacetamide or N-methylpyrrolidone in the presence of an organic base catalyst, after the reaction is terminated, the reaction is quenched with aqueous hydrochloric acid solution, then water is added for crystallization, and dimethyl curcumin is obtained; wherein the molar ratio of hydrochloric acid to organic weak base is (1-2): 1; the amount of water added is 1-3 times of the volume of the reaction solution. The method of the present invention is simple to operate, short in reaction time, convenient for post-processing, high in product yield and purity, environmentally friendly, and suitable for industrialized large-scale production.

The invention relates to the technical field of preparation of drugs and isomers thereof, and discloses a chemical synthesis method of a macloxvir diastereoisomer, which comprises the following steps: (A) condensing a compound shown as a formula I and a compound shown as a formula II to obtain a compound shown as a formula III; (B) removing an n-hexyl protecting group from the compound shown in the formula III under the action of lithium chloride to obtain a compound shown in a formula IV; and (C) carrying out substitution on the compound shown in the formula IV and chloromethyl methyl carbonate under an alkaline condition to obtain the macavir diastereoisomer. By adopting the synthesis method disclosed by the invention, large-batch preparation of the mabaloxvir diastereoisomers can be quickly realized, and the prepared product can be used as a reference substance for quality control of mabaloxvir bulk drugs.

The application discloses a synthesis method of a sulfentrazone intermediate. A compound shown in formula II is chlorinated in the presence of a solvent to synthesize a compound shown in formula I by adding a composite catalyst, wherein the composite catalyst comprises catalyst A and catalyst B in a weight ratio of 0.1-20:0.01-10; the catalyst A is one or both of a metal or a metal chloride; and the catalyst B is a compound capable of reacting with water to remove water in a reaction system. The method is simple in operation, easy to industrialize, relatively mild in reaction environment, cheap in catalyst, and high in reaction yield and product content.

The invention relates to a recombinant trichoderma reesei strain for producing brazitame protein as well as a construction method and application of the recombinant trichoderma reesei strain, and belongs to the technical field of biology. The recombinant trichoderma reesei strain is named as Trichoderma reesei DF230919, and is preserved in the China General Microbiological Culture Collection Center on August 9, 2024, and the preservation number of the recombinant trichoderma reesei strain is CGMCC (China General Microbiological Culture Collection Center) NO. 41488. According to the invention, a homologous recombination technology is utilized, QM6a [delta] pyr4 is taken as a host, a cellobiohydrolase gene cbh1 in trichoderma reesei is replaced with a Brazinein gene, and heterologous expression of Brazinein is realized in a recombinant trichoderma reesei strain; as the genome of the Trichoderma reesei is completely sequenced and widely researched, the construction and recombination of the Trichoderma reesei strain can be accurately controlled and regulated, the expression quantity and product purity of the Brazilian sweet protein can be effectively improved, and the Trichoderma reesei can grow under the condition of large-scale culture, so that the Trichoderma reesei strain can be widely applied to the field of culture of the Brazilian sweet protein. The Brazilian sweet protein is expressed in Trichoderma reesei through genetic engineering, so that the Brazilian sweet protein can be widely applied to industrial production of Brazilian sweet.

The invention discloses a high-purity ethyl sulfide rectification process and an integrated system thereof, and belongs to the technical field of chemical rectification. The high-purity ethyl sulfide rectification integrated system comprises a liquid injection device, a precise temperature control heating device, a rectification tower and an automatic shunting and collecting device which are connected in sequence, wherein the rectification tower and the automatic shunting and collecting device are connected in series; and the rectifying tower mainly comprises a plurality of rectifying columns which are arranged in parallel. According to the invention, the plurality of rectifying columns are connected in parallel and systematized and then connected in series for continuous rectification of ethanethiol, and the rectification purity of ethanethiol is continuously improved by means of multi-stage rectification and control of discharging at different reflux ratios at different temperatures, so that the defect that the high purity of 99% cannot be achieved in the existing ethanethiol rectification technology is overcome; the contradiction between the height, the pipe diameter and the like of a single rectifying column and the handling capacity is solved, and the requirements of high purity, high flux and high efficiency in the ethyl sulfide rectifying technology are met.

The invention relates to the technical field of antioxidants, and particularly discloses a synthesis process of an antioxidant 1135, which comprises the following steps: (1) adding 3, 5-methyl ester, a catalyst and a catalytic auxiliary agent into a reaction container, filling nitrogen for replacement, heating, starting stirring, keeping the temperature, and adding isooctanol for reaction; (2) after the reaction in the step (1) is finished, distilling to remove unreacted isooctanol, cooling, adsorbing by using an adsorption filter aid, and filtering to obtain the antioxidant 1135; the catalytic auxiliary agent in the operation is a composition of diatomite and porous alumina according to a weight ratio of (3-8): 1. When the synthesis process of the antioxidant 1135 is applied, not only is a relatively high catalytic reaction progress achieved, but also relatively high product yield and product purity can be shown.

This utility model discloses a high-efficiency plant active ingredient extraction and processing device, relating to the field of plant extraction. The device includes a grinding chamber, a feeding rack, a grinding plate, a leveling plate, and a driving device. The grinding chamber has a cavity with a fixed plate inside, dividing the cavity into an upper grinding chamber and a lower collecting chamber. The fixed plate has multiple collecting holes. The feeding rack is located on one side of the grinding chamber and has an inclined channel communicating with the cavity. The grinding plate is located at the top of the cavity. The leveling plate is located on the lower side of the grinding chamber. The driving device is located outside the grinding chamber and drives the leveling plate to move from the lower side of the grinding chamber to the other side, leveling the fruit peel material piled on the fixed plate. This utility model aims to automate the problem of uneven material distribution in traditional equipment.

The invention relates to an intelligent processing and extracting method for low-temperature extraction of walnut butter, and relates to the technical field of walnut butter extraction. The invention provides an intelligent processing and extracting method for extracting walnut butter at low temperature. According to the method, an ultrasonic-assisted low-temperature squeezing technology is utilized, so that the extraction rate and the quality of the walnut oil can be effectively improved, more nutritional ingredients are reserved, and low energy consumption and environment friendliness in the production process can be realized. The temperature, time and pressure of the whole extraction process are accurately controlled through an intelligent control system, the stability and repeatability of the extraction process are ensured, and an efficient, green and sustainable solution is provided for walnut oil production.

According to the preparation method of the levextrel resin and the application of the levextrel resin in purification of ammonium rhenate, the levextrel resin with excellent performance is prepared by setting the ratio of the oil-phase mixed solution to the water-phase mixed solution and accurately controlling the temperature. According to the extraction resin, the extraction agent is uniformly dispersed, the binding force with the resin is strong, and the loss is not easy. When the extraction resin is used for purifying an ammonium rhenate solution in a chromatographic column, ammonium rhenate with the purity larger than 99.99% can be obtained by controlling the concentration and the flow speed of the solution. The extraction resin prepared by the method provided by the invention not only can improve the purification efficiency and purity of ammonium rhenate, but also can reduce the generation of organic waste liquid and reduce the influence on the environment, and meanwhile, the synthesis and regeneration process of the resin is simplified, so that the economical efficiency and environmental protection property of an ammonium rhenate refining technology are improved; and a high-quality rhenium metal resource guarantee is provided for the development of the aerospace industry in China.

The utility model relates to the technical field of nanometer magnesium oxide reaction, in particular to a reaction device for preparing nanometer magnesium oxide from forsterite, which comprises a first reactor, a feeding pipe is mounted at one end of the first reactor, a second reactor is mounted at the other end of the first reactor, and a third reactor is mounted at one end of the second reactor; heating pipelines are mounted on the surfaces of the first reactor, the second reactor and the third reactor, heaters are mounted at one ends of the heating pipelines, supporting plates are mounted in inner cavities of the heating pipelines, and fans are mounted on the surfaces of the supporting plates; the inner cavities of the first reactor, the second reactor and the third reactor are respectively provided with a blanking mechanism, the top end of each blanking mechanism is provided with a plurality of catalyst carriers, and the top end of each catalyst carrier is provided with a plurality of round holes. The synthesis of the nano magnesium oxide can be carried out under the optimal temperature condition, and the yield is effectively improved.

The invention discloses a method for preparing vanadium trioxide based on ammonium polyvanadate and vanadium trioxide, and the method comprises the following steps: S1, pre-calcining and decomposing: uniformly laying ammonium polyvanadate in a calcining kiln, and calcining in an oxidizing atmosphere or inert atmosphere at 400-650 DEG C to obtain powdery vanadium pentoxide; s2, reduction: collecting the powdery vanadium pentoxide, feeding the powdery vanadium pentoxide into a reduction kiln, carrying out a reduction reaction in a reducing atmosphere at 600-800 DEG C, and keeping a solid-gas reaction state all the time in the reduction process to obtain vanadium trioxide powder; and S3, post-treatment is conducted, specifically, the reduced vanadium trioxide powder is cooled and sieved, and then a final product is obtained. According to the method, APV is converted into powdery V2O5 by introducing a pre-calcination decomposition procedure, and the powdery V2O5 is quickly reduced into a low-valence vanadium oxide with a high melting point after entering a reduction kiln, so that the possibility of ring formation of the reduction kiln is eliminated from the source, and the reduction reaction efficiency and the product purity are synchronously improved.

The invention provides radioisotope carbon-14 labeled methoprene and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing [cyano-14C] (R)-7-methoxy-3, 7-dimethyl octanenitrile; s2, preparing [aldehyde-14C] citronellal; and S3, preparing the carbon-14 labeled methoprene. The related field blank of radioisotope carbon-14 labeled methoprene is filled, accurate and reliable tracing information can be provided when behaviors of methoprene in organisms and environments are researched, a powerful tool is provided for deeply exploring the metabolic process, environmental behaviors, pharmacokinetics and the like of methoprene, and the method has the advantages of being high in practicability and wide in application prospect. And the development of research in related fields is promoted, such as optimization of the use strategy of methoprene in agricultural pest control.

This invention provides a cefotaxime hydrochloride reaction vessel, belonging to the field of pharmaceutical and chemical equipment technology, comprising: an acylation condensation vessel, a cryogenic esterification vessel, a controller, a lifting frame, a feed pipe, a transfer pipe, a drying sleeve, and a buffer isolation box; the acylation condensation vessel is positioned at a high position on one side of the cryogenic esterification vessel, and a lifting frame is installed at the bottom of the acylation condensation vessel, with a lifting component on the lifting frame. A vertically sliding and sealed acylation base is located at the bottom of the acylation condensation vessel, and the lifting component is used to control the vertical displacement of the acylation base. This invention utilizes a liftable acylation base in conjunction with a liquid level sensor and an organic phase concentration sensor to precisely adjust the reaction liquid level, ensuring that the feed pipe inlet is always immersed in the organic phase layer. Simultaneously, with the aid of a nitrogen-assisted jet, efficient and selective transfer of the organic phase is achieved, effectively preventing the aqueous phase from entering subsequent systems, thus solving the problem of incomplete phase separation during dual-vessel transfer when a single vessel cannot complete the two-step reaction.

The invention provides a method for reversible disulfide bond exchange mediated by free radicals. According to the method, hydroxyl (p-toluenesulfonyloxy) iodobenzene (HTIB) is used as a free radical initiator, and controllable exchange of disulfide bonds is realized under the condition of biological compatibility. The technology has wide application potential, and is especially suitable for the field of biological medicines, such as chemical modification of amino acids, peptides, carbohydrates, proteins and drug molecules. Different from a traditional transition metal catalyzed disulfide bond exchange method, the method has the advantages that the reversible disulfide bond exchange reaction is efficiently realized under mild and biocompatible conditions, the method is applicable to the fields of protein engineering, drug development, dynamic covalent chemistry and the like, and a new way is provided for synthesis and modification of biomolecules. # imgabs0 #

This invention relates to the field of organic synthesis technology, and discloses a copper-based catalyst, its preparation method, and a method for catalyzing the preparation of cyclododecanol from cyclododecanol into cyclododecanone. The preparation method of the copper-based catalyst includes the following steps: preparing a catalyst with a specific surface area of ​​200-250 m² / g. 2 / g Al2O3-SiO2 composite support; copper source and rare earth oxide additives were loaded onto the Al2O3-SiO2 composite support, dried and calcined to obtain catalyst precursor; the catalyst precursor was reduced to prepare Cu + A copper-based catalyst with a molar ratio of 1.8 to 2.5:1 to elemental copper. This invention can control the Cu content in the copper-based catalyst. + The molar ratio with elemental copper allows the catalyst's activity state to be adjusted according to different production needs, thereby enabling flexible control of the reaction rate, improving process adaptability and operational stability; at the same time, it helps to suppress side reactions and improve the selectivity and purity of cyclododecanone.