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New wild blueberry anthocyanin extraction and purification method

A blueberry anthocyanin and new method technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of low yield and low purity, achieve high product purity, overcome low purity, and improve extraction. effect of speed

Inactive Publication Date: 2014-10-22
DAXINGANLING SHENZHOU NORTH POLE BLUEBERRY BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantages of the present invention: the extraction speed is greatly improved, and at the same time, the content of the effective components of anthocyanin is greatly increased, and the disadvantages of low purity and low yield of the conventional extraction are overcome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: A new method for finally obtaining high-purity blueberry anthocyanins by adopting biological enzyme method, ionic liquid microwave extraction, ultrafiltration membrane concentration coupling high-speed countercurrent chromatography purification, and its steps are as follows:

[0020] (1) Biological enzymatic hydrolysis: Take 10kg of fresh blueberry fruit, crush it, add pectinase and cellulase to enzymolyze, the amount of enzyme added is 0.2g / kg, the enzymolysis temperature is 45°C, and the enzymolysis time is 2h;

[0021] (2) Microwave reflux extraction: the mixture after enzymolysis is added to 1-alkyl-3-methylimidazolium tetrafluoroborate ionic liquid aqueous solution with a concentration of 60% by volume, and the microwave heating reflux extraction method is adopted, and the microwave power is 400W , frequency 5000MHz, ratio of solid to liquid 1:5, extraction temperature 55°C, extraction twice, each extraction time 0.5h;

[0022] (3) Collection: reclaim...

Embodiment 2

[0030] Embodiment 2: A new method for finally obtaining high-purity blueberry anthocyanins by adopting biological enzyme method, ionic liquid microwave extraction, ultrafiltration membrane concentration coupling high-speed countercurrent chromatography purification, and its steps are as follows:

[0031] (1) Biological enzymatic hydrolysis: Take 50kg of fresh blueberry fruit, mix pectinase and cellulase for enzymolysis, the enzyme addition amount is 0.25g / kg, the enzymatic hydrolysis temperature is 50°C, and the enzymatic hydrolysis time is 3h;

[0032] (2) Microwave reflux extraction: add the mixture after enzymolysis to 70% volume percent concentration of 1-butyl-3-methylimidazolium chloride salt ionic liquid aqueous solution, adopt microwave heating reflux extraction method, microwave power is 600W, frequency 8000MHz , the ratio of solid to liquid is 1:8, the extraction temperature is 60°C, two extractions, each extraction time is 1h;

[0033] (3) Collection: reclaim the io...

Embodiment 3

[0041] Embodiment 3: A new method for finally obtaining high-purity blueberry anthocyanins by adopting biological enzyme method, ionic liquid microwave extraction, ultrafiltration membrane concentration coupling high-speed countercurrent chromatography purification, and its steps are as follows:

[0042] (1) Biological enzymatic hydrolysis: take 1kg of fresh blueberry fruit, mix pectinase and cellulase for enzymolysis, the enzyme addition amount is 0.3g / kg, the enzymatic hydrolysis temperature is 50°C, and the enzymatic hydrolysis time is 2 hours;

[0043] (2) Microwave reflux extraction: add the liquid after enzymolysis to 50% 1-hexyl-3-methylimidazolium hexafluorophosphate ionic liquid aqueous solution, adopt microwave heating reflux extraction method, microwave power is 500W, frequency 10000MHz, material-to-liquid ratio 1:10, extraction temperature 55°C, extraction twice, each extraction time 1h;

[0044] (3) Collection: reclaim the ionic liquid, detect and analyze by thin-...

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Abstract

The invention belongs to the field of the natural organic chemistry, relates to a method for extracting and purifying anthocyanins from wild blueberries in Greater Khingan, and particularly relates to a new method adopting a biological enzyme process, ionic liquid microwave extraction, and ultrafiltration membrane concentration coupled high-speed countercurrent chromatography purification to finally obtain highly pure blueberry anthocyanins. The method has the following advantages: 1, an ionic liquid has a very strong microwave absorption ability, so the ionic liquid microwave extraction process is fast and efficient, has a simple and easy process, fully extracts the blueberry anthocyanins effective ingredients, and realizes a high content of the anthocyanins effective ingredients; and 2, compared with traditional solid-liquid chromatography technology, the high-speed countercurrent chromatography purification technology has the advantages of flexible operation, high efficiency, rapid separation speed, large preparation amount and low cost, and can be industrially used. The blueberry anthocyanins obtained by using the method have high content and high activity, and has a purity reaching 42.7%.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a new method for extracting and purifying blueberry anthocyanins from Daxinganling wild blueberries, in particular to a new method for extracting and purifying blueberry anthocyanins using ionic liquid microwaves and high-speed countercurrent chromatography method. Background technique: [0002] Blueberry (Blueberry) scientific name Vaccinium, Rhododendron Vaccinium plant. Most of them grow in the primeval forests north of Daxing'an Mountains in my country. The fruit is blue in color, with delicate flesh, sweet and sour taste, unique flavor and rich nutrition. The unique health care effect of blueberry anthocyanin on human vision makes it very popular in health food and medical circles. As a natural food coloring, blueberry anthocyanin is safe, non-toxic, rich in resources, and has certain nutritional and pharmacological effects. It has great application potential in fo...

Claims

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Application Information

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IPC IPC(8): C09B61/00
Inventor 张殿臣张军
Owner DAXINGANLING SHENZHOU NORTH POLE BLUEBERRY BIOLOGICAL ENG
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