New wild blueberry anthocyanin extraction and purification method
A blueberry anthocyanin and new method technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of low yield and low purity, achieve high product purity, overcome low purity, and improve extraction. effect of speed
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[0019] Example 1: A new method for obtaining high-purity blueberry anthocyanins by adopting biological enzymatic method, ionic liquid microwave extraction, and ultrafiltration membrane concentration lotus-linked high-speed countercurrent chromatography purification to finally obtain high-purity blueberry anthocyanins. The steps are as follows:
[0020] (1) Biological enzymatic hydrolysis: take 10kg of fresh blueberry fruit, add pectinase and cellulase mixed enzymatic hydrolysis after crushing, add 0.2g / kg enzyme, enzymatic hydrolysis temperature 45℃, enzymatic hydrolysis time 2h;
[0021] (2) Microwave reflux extraction: add the mixture after enzymatic hydrolysis to a 60% volume percent aqueous solution of 1-alkyl-3-methylimidazole tetrafluoroborate ionic liquid, and use microwave heating reflux extraction method with a microwave power of 400W , Frequency 5000MHz, material-liquid ratio 1:5, extraction temperature 55℃, extraction twice, each extraction time 0.5h;
[0022] (3) Collecti...
Example Embodiment
[0030] Example 2: A new method for obtaining high-purity blueberry anthocyanins by adopting biological enzymatic method, ionic liquid microwave extraction, ultrafiltration membrane concentration and lotus-linked high-speed countercurrent chromatographic purification, and the steps are as follows:
[0031] (1) Biological enzymatic hydrolysis: take 50kg of fresh blueberry fruit, mix pectinase and cellulase for enzymatic hydrolysis, add 0.25g / kg enzymatic hydrolysis temperature 50℃, enzymatic hydrolysis time 3h;
[0032] (2) Microwave reflux extraction: add the mixture after enzymatic hydrolysis to a 70% volume percent concentration of 1-butyl-3-methylimidazolium chloride salt ionic liquid aqueous solution, use microwave heating reflux extraction method, microwave power 600W, frequency 8000MHz , The material-to-liquid ratio is 1:8, the extraction temperature is 60℃, and the extraction time is 1h twice;
[0033] (3) Collection: Recover the ionic liquid, detect and analyze by thin layer c...
Example Embodiment
[0041] Example 3: A new method for obtaining high-purity blueberry anthocyanins by adopting biological enzymatic method, ionic liquid microwave extraction, ultrafiltration membrane concentration and lotus-linked high-speed countercurrent chromatography purification, and the steps are as follows:
[0042] (1) Biological enzymatic hydrolysis: take 1kg of fresh blueberry fruit, mix pectinase and cellulase for enzymatic hydrolysis, add 0.3g / kg enzymatic hydrolysis temperature 50℃, enzymatic hydrolysis time 2h;
[0043] (2) Microwave reflux extraction: add the liquid after enzymatic hydrolysis to a 50% volume percent aqueous solution of 1-hexyl-3-methylimidazole hexafluorophosphate ionic liquid, use microwave heating reflux extraction method, microwave power 500W, frequency 10000MHz, material-to-liquid ratio 1:10, extraction temperature 55℃, extraction twice, extraction time 1h each time;
[0044] (3) Collection: Recover the ionic liquid, detect and analyze by thin layer chromatography, a...
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