Gene chip capable of simultaneously detecting human herpes virus and enterovirus
A human herpes virus and gene chip technology, applied in the field of molecular biology, can solve the problems of unsuitable virus extraction, virus loss, cumbersome operation, etc., and achieve the effect of quick extraction, small sample size and high amplification efficiency
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[0073] Example 1
[0074] A gene chip for simultaneous detection of human herpesvirus and enterovirus in cerebrospinal fluid, including a nucleotide extraction kit, a DNA and RNA simultaneous reverse transcription kit, a DNA and RNA simultaneous PCR amplification kit, and a specific probe connected Gene chip
[0075] The specific reverse transcription primer sequence in the DNA and RNA simultaneous reverse transcription kit is as follows:
[0076] EV-r: 5′-TGACTCATCGACCTGATCTACA-3′;
[0077] The primers in the DNA and RNA simultaneous PCR amplification kit are composed of Biotin-HHV1,2,4,5-f, HHV1,2,5-r, HHV4-r, Biotin-EV-f and EV-r. The primers are mixed in a ratio of 4.5:0.6:0.2:4.5:0.6, and the nucleotide sequences are as follows:
[0078] HHV1,2,4,5-f:5′-TCATCTACGGGGACACGGAC-3′
[0079] HHV1,2,5-r: 5′-CGCACCAGATCCACGCCCTT-3′
[0080] HHV4-r: 5′-GAGCTCCACCCCCTTCATC-3′
[0081] EV-f: 5′-TTAAAACAGCCTGTGGGTTG-3′
[0082] EV-r: 5′-TGACTCATCGACCTGATCTACA-3
[0083] Among them, HHV1,2,4,5-f a...
Example Embodiment
[0120] Example 2
[0121] A gene chip for simultaneous detection of human herpesvirus and enterovirus in cerebrospinal fluid, including a nucleotide extraction kit, a DNA and RNA simultaneous reverse transcription kit, a DNA and RNA simultaneous PCR amplification kit, and a specific probe connected Gene chip; characterized by:
[0122] The specific reverse transcription primer sequence in the DNA and RNA simultaneous reverse transcription kit is as follows:
[0123] EV-r: 5′-TGACTCATCGACCTGATCTACA-3′;
[0124] The primers in the DNA and RNA simultaneous PCR amplification kit are composed of Biotin-HHV1,2,4,5-f, HHV1,2,5-r, HHV4-r, Biotin-EV-f and EV-r. The primers are mixed in a ratio of 4.5:0.6:0.2:4.5:0.6, and the nucleotide sequences are as follows:
[0125] HHV1,2,4,5-f:5′-TCATCTACGGGGACACGGAC-3′
[0126] HHV1,2,5-r: 5′-CGCACCAGATCCACGCCCTT-3′
[0127] HHV4-r: 5′-GAGCTCCACCCCCTTCATC-3′
[0128] EV-f: 5′-TTAAAACAGCCTGTGGGTTG-3′
[0129] EV-r: 5′-TGACTCATCGACCTGATCTACA-3
[0130] Among t...
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