16 results about "DNA virus" patented technology

The present invention is directed to a method of determining DNA concentration in a DNA virus. The invention features three basic steps. The initial step is the capture of a defined amount of virus capsid particles on a first solid phase. The second step is lysis of the capsid to release the virus DNA from the first solid phase into a lysis solution. After separating the lysis solution from the first solid phase, the third step is contacting the lysis solution with a second solid phase. The second solid phase captures total DNA derived from the captured capsid. The present invention is also directed to a method for measuring the percentage of full virus capsid, comprising first determining the ssDNA concentration in viruses, and then converting the ssDNA concentration to percentage of full virus capsid using a calibration curve having DNA concentration plotted against standards of % of full capsids.

The application provides a DNA virus tracer coated with a protein by an EDC covalent coupling method and a preparation method and application thereof. The DNA virus tracer coated with the protein of the application is composed of a core, a shell and a protein shell coated on the shell; the core is a SiO2 nanosphere with adsorbed nucleic acid on the surface, a layer of SiO2 shell is coated on the outside of the core, the surface of the shell is modified by carboxylation, and the shell is formed by covalently coupling the protein through EDC. The novel DNA virus tracer provided by the application can effectively protect the coding DNA, facilitate subsequent quantitative detection and analysis, effectively ensure that the outermost shell of the tracer is completely composed of the selected protein, and make the surface characteristics and migration characteristics of the tracer closer to the target virus.

The invention discloses an antiviral polypeptide and application thereof. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1 or 2. The invention discovers that the antiviral polypeptide shows remarkable broad-spectrum inhibitory activity on DNA (deoxyribonucleic acid) virus (HSV-1), RNA (ribonucleic acid) virus (H1N1) and enveloped virus (VSV), and the inhibition rates of the antiviral polypeptide on the DNA virus (HSV-1), the RNA virus (H1N1) and the enveloped virus (VSV) respectively reach 50.54%-75.18%, 73.06%-96.30% and 64.78%-98.68% under the concentration of 10 mu M. Meanwhile, the polypeptide has excellent safety, the half toxicity concentration in each cell line is higher than 50 mu M, and the cell survival rate under the effective antiviral concentration exceeds 90%. The invention effectively overcomes the technical defects of narrow spectrum, easy generation of drug resistance and insufficient safety of the existing antiviral drug, and provides an important candidate molecule for developing a new generation of antiviral drugs.

A combination therapeutic strategy employs multiple oncolytic viruses (OVs), grouped and administered based on their virological properties, tumor selectivity, and distinct antigen profiles, to treat malignant tumors. Representative groupings may include members of flaviviruses, each contributing distinct immune modulation and the same modes of tumor cell killing. A predefined treatment schedule involves sequential or concurrent administration of antigenically diverse OVs in multiple cycles, reducing cross-neutralization and sustaining cytolytic pressure on tumors. A pre-characterized OV panel, comprising members from a virus family, RNA and DNA viruses, both wild-type and genetically engineered, serves as a flexible resource for customizing treatment regimens by tumor type, immune landscape, and therapeutic goals. This platform establishes a rational framework for combination OV therapy with improved durability, safety, and clinical effectiveness.

This invention belongs to the technical field of virus detection, specifically relating to a PCR detection kit for the simultaneous detection of four DNA viruses in waterfowl and its application. The PCR detection primer set for the simultaneous detection of four DNA viruses in waterfowl of this invention is the optimal primer set obtained through experimental screening. The kit composed of these primers can rapidly and specifically amplify and diagnose Muscovy duck parvovirus, duck plague virus, duck adenovirus type 3, and duck circovirus simultaneously, exhibiting high specificity and sensitivity. It can accurately and rapidly identify MDPV, DPV, DAdV-3, and DuCV, and can complete quantitative real-time PCR detection in approximately 50 minutes. The kit is simple and practical to operate, achieving efficient simultaneous detection of four viruses, shortening the detection cycle, increasing sensitivity by 10-1000 times, significantly improving early disease diagnosis capabilities, significantly reducing the misdiagnosis rate of diseases in poultry farms, and contributing to the sustainable development of the poultry farming industry.

This invention relates to the field of pharmaceutical technology, specifically to the application of 3-hydroxydecanoic acid in the preparation of antiviral drugs. In cell experiments, 3-hydroxydecanoic acid showed significant inhibitory effects on a variety of viruses, including RNA viruses (BEFV and BVDV) and DNA viruses (HSV-1 and BoHV-1), indicating that it has inhibitory effects on multiple viruses, is less likely to induce drug resistance, and has the advantage of broad-spectrum antiviral activity.

The invention relates to a non-guanidine salt type compatible nucleic acid extraction virus preservation solution as well as a preparation method and application thereof, and the virus preservation solution comprises P (HEMA-BAMPD hydrochloride), a buffer agent, a chelating agent, a saccharide stabilizer and an osmotic pressure regulator. The P (HEMA-BAMPD hydrochloride) is used for replacing guanidine salt and small molecule quaternary ammonium salt, the inactivation rate effect on enveloped RNA / DNA viruses such as SARS-CoV-2 viruses and influenza viruses is good, no irritation is generated, and the method is friendly to operators and the environment; the macromolecular structure realizes multi-point adsorption and has strong organic matter interference resistance, and a small amount of blood, sputum and other components in the sample do not influence the inactivation effect. Moreover, the P (HEMA-BAMPD hydrochloride), the chelating agent and the saccharide stabilizer have a synergistic effect, so that the activities of RNase and DNase can be effectively inhibited.

Macrophages (Mφ) are crucial for pathogen detection and elimination, and also serve as a microenvironment for replication of multiple infectious pathogens. This delicate balance between viral replication and antiviral response remains largely unknown at the molecular level. Addressing this issue is physiologically important given the persistent threat posed by emerging viral pathogens. Here, we demonstrate that GAS7 expression within macrophages restricts the replication of viral pathogens belonging to all major viral groups. We show that the antiviral activity of GAS7 exists even under conditions where the classical antiviral response mediated by type I interferon is neutralized. In particular, we show that in human monocyte-derived macrophages, silencing of GAS7 boosts the replication of multiple viral pathogens representing most relevant viral groups. These include the retroviruses HIV-1 (human immunodeficiency virus type 1) and HIV-2 (human immunodeficiency virus type 2), the RNA viruses Zika virus (positive-sense single-stranded RNA), Sindbis virus (single-stranded positive-sense RNA), Sendai virus (negative-sense single-stranded RNA), VSV virus (negative-sense single-stranded RNA), and measles virus (negative-sense single-stranded RNA), and the DNA virus HSV-1 virus (double-stranded DNA). Importantly, the antiviral activity of GAS7 is present even under conditions where type I interferon is neutralized by the addition of the B18R protein (which efficiently blocks the type I receptor). Furthermore, the inventors demonstrate that by forcing macrophages to overexpress GAS7, they are further protected from HIV-1 infection compared to cells expressing this factor at normal levels. Therefore, the present invention relates to the modulation of GAS7 expression and / or activity for modulating viral replication in macrophage populations.

The invention belongs to the technical field of virus detection, and discloses a reaction solution for DNA virus and RNA virus detection, the reaction solution is a reaction solution containing acetate, acetate salt is introduced as an ionic reaction system, the specificity of a one-step method RT-PCR amplification system is greatly improved, and the specificity of the one-step method RT-PCR amplification system is greatly improved. The amplification inhibition phenomenon of the residual reverse transcriptase activity on a subsequent DNA template is greatly reduced, so that the kit has a good effect on DNA detection while being capable of detecting the RNA virus.

This invention belongs to the field of biomedicine and provides the application of LAPF in the preparation of antiviral drugs. This invention enhances lysosomal acidification in dendritic cells or macrophages by administering LAPF protein, nucleic acid molecules encoding LAPF, or their functional enhancers, thereby promoting TLR9 transport to lysosomes and activating the type I interferon pathway. Experimental results demonstrate that administering LAPF protein, nucleic acid molecules encoding LAPF, or their functional enhancers can significantly reduce the viral load of DNA viruses such as HSV-1, increase the survival rate of infected mice, and enhance IFN-α / β production, exhibiting a good antiviral immune-enhancing effect.

The invention discloses a triple RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) method and a kit for simultaneously detecting IMNV (Immunodeficiency Virus), CMNV (Cytomegalovirus) and DIV1 (Deoxyribose Immunodeficiency Virus According to the invention, PCR primers and TaqMan probes for detecting IMNV, CMNV and DIV1 are respectively designed, and the nucleotide sequences of the PCR primers and TaqMan probes are shown as SEQ ID NO: 1-9. The primer and the probe can be used for simultaneously qualitatively and quantitatively detecting the infectious muscle necrosis virus, the secretly death nodavirus and the full-eye iridovirus 1 through a triple RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) method. According to the invention, common detection of RNA virus and DNA virus is realized for the first time, the kit has the characteristics of high efficiency and convenience, and the advantages of high sensitivity, high specificity and quantitability, and meanwhile, due to the addition of the molecular crowding agent, the detection efficiency and detection speed of medium and low-concentration virus load can be effectively improved, and the detection cost is reduced. The invention provides a convenient and reliable detection method and product for early monitoring, early warning and diagnosis of the three virus diseases of the penaeus vannamei boone.

The application discloses a method for identifying DNA viruses in organisms based on high-throughput sequencing data, and the method comprises the following steps: taking a biological sample derived from the organism as a to-be-tested sample, performing high-throughput sequencing on the to-be-tested sample, and obtaining sequencing data in a FastQ file format; performing analysis and identification by using the sequencing data; and then identifying the DNA viruses in the to-be-tested sample. The method does not need to rely on metagenomic sequencing, can accurately identify the DNA viruses by using sequencing data of second-generation sequencing (high-throughput sequencing), avoids the generation of false positives, and greatly improves the utilization rate of sample sequencing data.

The invention discloses application of Momordin-Ic and I-type interferon in preparation of antiviral drugs, and relates to the technical field of molecular biology. It is found that Momordin-Ic has a significant antiviral function, can significantly inhibit replication of various RNA viruses and DNA viruses, and is verified in various cell lines and mice. Compared with the single use of the I-type interferon, the combination of the Momordin-Ic and the I-type interferon can further enhance the antiviral activity of the drug. Therefore, Momordin-Ic is expected to become a broad-spectrum antiviral drug for improving the antiviral activity of clinical interferon.

The present application relates to a kind of non-guanidine salt type compatible nucleic acid extraction virus preservative and preparation method and application, the virus preservative, including P (HEMA-BAMPD) hydrochloride, buffer, chelating agent, saccharide stabilizer and osmotic pressure regulator.The present application replaces guanidine salt and small molecule quaternary ammonium salt with P (HEMA-BAMPD) hydrochloride, and the inactivation rate effect of SARS-CoV-2 virus, influenza virus and other envelope type RNA / DNA viruses is good, and there is no irritability, and it is friendly to operating personnel and environment;Polymer structure realizes multiple point adsorption, and the ability of resistance to organic interference is strong, and a small amount of blood, sputum and other components in sample do not affect inactivation effect.Moreover, P (HEMA-BAMPD) hydrochloride, chelating agent and saccharide stabilizer synergistic effect can effectively inhibit RNase, DNase activity.

The application discloses a kind of graft injury multi-index combined noninvasive detection kit, by optimized multiplex real-time fluorescent quantitative polymerase chain reaction technology, realize the quantitative detection of six categories with closely related DNA virus after transplantation Complications, the present application relates to the field of in vitro detection technology of clinical sample.The graft injury multi-index combined noninvasive detection kit, the present application first realizes the synchronous detection of BK virus, JC virus, B19 virus, CMV virus, adenovirus and six kinds of closely related viruses of circovirus (TTV) with graft injury.Test data see example 7, from the detection results of 100 clinical kidney transplant patients, it can be seen that the kit of the present application can simultaneously accurately detect the load level of six viruses, to provide comprehensive infection risk assessment for clinicians.Compared with the prior art single virus detection method, the present application avoids the cumbersome process of multiple sampling and detection, significantly improves the clinical work efficiency.

The application discloses a Macrobrachium rosenbergii circular DNA virus and a detection primer and kit thereof, is classified and named as Macrobrachium rosenbergii circular rep-encoding single-strand DNA virus, is preserved in a preservation unit of China General Microbiological Culture Collection Center, has a preservation number of CGMCC NO:46279, and is preserved on December 19, 2024.The application is closely related to the virus of the slow growth symptom of Macrobrachium rosenbergii, provides a research basis for prevention and treatment of Macrobrachium rosenbergii iron shrimp syndrome, simultaneously develops virus detection related technology, fills the blank that there is no nucleic acid molecule detection method of the Macrobrachium rosenbergii circular DNA virus at present, and is suitable for diagnosis, screening and prevention of the Macrobrachium rosenbergii circular DNA virus.