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Grouper piscidin 4 polypeptide and application thereof

A grouper and medicine technology, which is applied in the field of genetic engineering to achieve the effects of inhibiting the replication of DNA viruses and RNA viruses and inhibiting the growth of bacteria

Active Publication Date: 2016-07-27
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the role of grouper piscidin4 in anti-fish virus has not been reported

Method used

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  • Grouper piscidin 4 polypeptide and application thereof
  • Grouper piscidin 4 polypeptide and application thereof
  • Grouper piscidin 4 polypeptide and application thereof

Examples

Experimental program
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Embodiment 1

[0029] 1. Artificial synthesis of grouper piscidin4 polypeptide

[0030] (1), according to the grouper piscidin4 gene fragment in the database, design specific primers to amplify the open reading frame sequence of grouper piscidin4, design the following specific primers: piscidin4-orf-s: 5'-ATGAAGTTTGTTATGGTCTTTCTGG-3' (specific shown in SEQ ID NO.1); piscidin4-orf-r: 5'-TCATTTCTGCCAGTATGGCGGAGTT-3' (specifically shown in SEQ ID NO.2).

[0031] (2), extract grouper spleen tissue RNA, and use RNA as template to synthesize cDNA with reverse transcriptase, take cDNA as template, adopt the specificity primer designed in step (1), in the PCR amplification system for stone The open reading frame sequence of the grouper fish piscidin4 gene was amplified, and the purified gene fragment was recovered at the same time.

[0032] (3) After the PCR product is purified by the DNA Fragment Gel Recovery Kit, it is connected to the pMD18-T vector, transformed into E.coliDH5α competent cells, ...

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Abstract

The invention discloses a grouper piscidin 4 polypeptide and application thereof.The amino acid sequence of the grouper piscidin 4 polypeptide is shown in SEQ ID NO.7.The grouper piscidin 4 polypeptide is antibacterial peptide with the antibacterial and antiviral activity.The grouper piscidin 4 polypeptide has the advantages of inhibiting bacterium growth and inhibiting DNA virus and RNA virus replication, and can be used for preparing antibacterial medicine and antivirus medicine.The research on the grouper piscidin 4 polypeptide has great scientific value and application value for further researching and developing disease-resistant gene replication.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a grouper piscidin4 polypeptide and an application thereof. Background technique: [0002] Antimicrobial Peptide (AMP) is a class of endogenous natural biological small molecular peptides with broad-spectrum antibacterial activity, widely exists in the biological world, and has become an important component in the evolution of non-specific immunity. Piscidin is a typical amphipathic α-helical cationic peptide, which has a strong inhibitory effect on a variety of fish pathogenic bacteria, and has an inhibitory effect on viruses, fungi and even parasites. Piscidin widely exists in bony fishes (especially Perciformes), and is a family of bony fish antimicrobial peptides that deserves special attention. At present, the phenomenon of multiple isoforms of piscidin gene has been found in many fishes. [0003] Grouper (Epinepheluscoioides) is an important economic species...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C12N15/12A61K38/17A61P31/04A61P31/12
CPCA61K38/00C07K14/461
Inventor 魏京广秦启伟黄友华
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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