202 results about "RNA virus" patented technology

The invention discloses application of a small molecule compound in prevention and treatment of plant RNA virus infection, and belongs to the technical field of biology. According to the invention, 2 '-amino-2'-deoxyguanosine (ADG) is used as a unique antiviral active component to be applied to prevention and treatment of plant RNA viruses for the first time, and broad-spectrum inhibition of various plant RNA viruses such as turnip mosaic virus (TuMV), tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV) can be realized only by a low concentration of 250 [mu] M and a simple exogenous spraying mode; and after spraying, the normal growth of plants is not influenced, the risk of phytotoxicity is thoroughly avoided, the safety, high efficiency and broad spectrum of plant RNA virus prevention and control are remarkably improved, and important practical application significance is achieved for guaranteeing the yield and quality of crops in agricultural production and promoting agricultural green transformation.

The invention belongs to the technical field of biotechnology and genetic engineering, and particularly relates to application of recombinant RASA3 in preparation of a medicine for preventing and treating viral pneumonia. The recombinant RASA3 is used for promoting autophagy degradation of a transcription factor CEBP / beta protein and reducing the expression level of the transcription factor CEBP / beta protein, so that generation of inflammatory factors such as IL-6 is reduced, and inflammatory damage caused by excessive antiviral innate immune response in the virus infection process is improved. The invention provides a novel treatment strategy for inflammatory diseases caused by RNA viruses such as influenza and respiratory syncytial virus infection.

A nucleic acid extraction composition, reagents and kits containing the same and uses thereof. Provided is a nucleic acid extraction and purification reagent free of volatile organic solvents, which prevents the damage of volatile organic solvents to people and greatly improves the timeliness of nucleic acid extraction and purification, making the operation extremely simple, and the nucleic acid can be obtained within 10 minutes. The obtained nucleic acid may be used for biological reactions such as PCR, NASBA, LAMP and RPA. Moreover, the reagents of the present disclosure may be used to extract nucleic acids of cells, bacteria, fungi, DNA viruses and RNA viruses from various samples such as blood, throat swab preserving fluid, saliva, urine, sputum, excrement and the like, and very suitable for clinical and scientific research uses.

The invention provides a leaf vegetable silencing system based on RNA virus induction and a construction method and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, pTRV2-BrPDS, pTRV2-BjuPDS-g and pTRV2-BrPDS-c silencing expression vectors are constructed, the silencing expression vectors are introduced into leaves of brassica juncea and brassica juncea, and a VIGS technology is successfully applied to silence endogenous PDS genes, so that brassica juncea and brassica juncea plants generate albino phenotypes of different degrees, technical support is provided for research on gene functions of brassica juncea and brassica juncea, and the application has a wide application prospect. The speed of research on functional genes of the brassica juncea and the brassica juncea can be

The present invention is directed to methods for the use of 5′-adenosine diphosphate ribose (ADPR), and compositions thereof, for treating, managing, or preventing RNA virus-related diseases or conditions, herpes virus related diseases or conditions, Sirtuin 6 (Sirt6)-related diseases or conditions, Pax6 related diseases or conditions, and p53 related diseases or conditions.

Synthetic Southern Phaseoloviridae satellite RNA molecules and satellite particles containing the same are disclosed. Synthetic Southern Phaseoloviridae satellite RNA molecules containing an internal heterologous RNA virus (HRV) amplicon are also disclosed. Also disclosed are methods of using Southern Phaseoloviridae satellite RNA molecules and satellite particles containing the same to alter plant phenotypes, improve stress resistance in plants, and improve pest and pathogen resistance in plants.

The application discloses a method for producing pectin oligosaccharides by microbial degradation of pomace and application thereof. The method comprises the following steps: adding microbacterium A5 to a culture medium containing pomace to perform fermentation to obtain a fermentation liquor, performing fractional pressing filtration and filter sterilization on the fermentation liquor, performing ethanol precipitation to extract a pectin crude product, removing protein, performing ion exchange chromatography, dialysis desalting, freeze drying and the like to purify two pectin oligosaccharides POS-1 and POS-2 with a purity greater than 95%; the pectin oligosaccharides POS-1 and POS-2 have strong scavenging capacity on hydroxyl radicals and DPPH free radicals and the capacity of inhibiting the activity of respiratory RNA viruses such as influenza virus H1N1 and respiratory syncytial virus RSV, and can be applied to medicines or health foods.

The invention provides a Marburg virus detection standard substance as well as a preparation system and application thereof, and belongs to the technical field of biological detection. The armored RNA standard substance containing the Marburg virus NP gene segment, provided by the invention, contains the NP gene segment, can be used for nucleic acid detection of the Marburg virus taking the NP gene as a target spot, and is used for quality control of detection methods and reagents such as common PCR (Polymerase Chain Reaction), fluorescent quantitative PCR, digital PCR, LAMP (Loop-Mediated Isothermal Amplification) and the like. The armored RNA standard substance provided by the invention has the advantages of high purity, no plasmid DNA residue and good quality control performance. The armored RNA virus-like particle simulates the structure of a natural virus, and can participate in links such as nucleic acid extraction and amplification as an animal body or human body blood sample containing Marburg virus, so that the whole-course quality control of the whole detection process is realized, and the authenticity and reliability of the detection result are ensured.

The invention belongs to the technical field of gene engineering, and particularly relates to a negative-sense RNA virus plant genome editing system and application thereof. According to the genome editing system, a CRISPR-Cas9 gene editing technology is used, a plant cytoplasm rhabdovirus is used as a delivery carrier, the rhabdovirus is spread to a plant by means of entomophilous, a gene editing component is delivered to an axillary meristematic zone by utilizing RNA (Ribonucleic Acid) to move a motif, and a mutant lateral bud is obtained along with plant tillering. According to the method, plant gene editing can be completed without tissue culture, plant tissue culture dependence is overcome, the range of gene-editable strains is expanded, and pure mutants can be obtained only by one generation without hybridization and selfing processes.

The invention discloses a plant endogenous small molecule polypeptide and application thereof in inhibiting RNA virus infected plants, the sequence of the small molecule polypeptide is Pep1-3: IIGVSNFDPVKRS, and the small molecule polypeptide is derived from nicotiana benthamiana; the invention relates to application of plant endogenous small molecule polypeptide in inhibiting RNA virus infection of plants. The plant is tobacco; the RNA viruses comprise tobacco mosaic viruses, tomato mosaic viruses and turnip mosaic viruses, and the small peptide Pep1-3 serves as a novel plant immunity inducer and shows the potential of the small peptide Pep1-3 in the aspect of preventing and controlling the RNA viruses. In agricultural production, prevention and treatment of virus diseases by adopting oligopeptide substances are a green and sustainable direction, and compared with traditional pesticides and plant immunity inducers, the composition has the advantages of broad spectrum, small toxicity, no drug resistance risk and long lasting period, and can generate a synergistic or synergistic effect when being mixed with other medicaments.

Provided herein are methods for treating a virus (e.g,. an RNA virus, such as a SARS-CoV-2) infection or a disease associated therewith (e.g., COVID-19) comprising administering Rigosertib or a composition thereof to a subject (e.g., a human subject).

The present invention relates to a novel purine derivative compound and a composition for enhancing an immune response comprising same as an active ingredient. The compound of the present invention not only has a nanomolar EC50 value for TLR7, which is an intracellular membrane receptor of immune cells, but also has high selectivity for TLR7 compared to TLR8, which has a similar structure and is mainly distributed in the endoplasmic reticulum (ER), thereby being able to induce sustained immune activation. Therefore, the compound of the present invention can be effectively used as an efficient vaccine adjuvant composition against various RNA viruses including influenza virus, SARS-CoV-2, and hepatitis C virus.

The invention discloses a mandarin fish medullary tissue cell line and a construction method and application thereof. The invention belongs to the technical field of aquatic organism cells, and discloses a mandarin fish medulla oblongata cell line MFSB, which is preserved in China Center for Type Culture Collection on December 4, 2024, has a preservation number of CCTCC NO: C2024376, and is named as mandarin fish medulla oblongata cell line MFSB. The construction method comprises the steps of primary culture of mandarin fish medulla oblongata tissue cells, subculture, cryopreservation and resuscitation of the cells and the like, and the mandarin fish medulla oblongata cell line MFSB has the advantages of stable subculture, strong cell line division ability, fast growth speed and good stability, can be used as a cell model or an experimental tool to be applied to scientific research and animal genetic breeding, and has wide application prospects. The probe has broad-spectrum sensitivity to mandarin fish infectious spleen and kidney necrosis virus, mandarin fish double-segment RNA virus, mandarin fish frog iridovirus and mandarin fish rhabdovirus, and can be well applied to separation, culture and detection of mandarin fish virus and research and development of vaccines.

The application discloses application of a fungal virus in prevention and treatment of pear tree and apple tree Valsa diseases. The fungal virus is a positive single-stranded RNA virus, and a cDNA nucleotide sequence of the fungal virus is shown as SEQ ID No. 1. A fungus containing the virus is Valsa pyri HJL1-22 (gamma), the fungus is infected by the fungal virus of claim 1, the fungus is named as HJL1-22 (gamma), is classified and named as Valsa pyri, and has a preservation number of CGMCC No. 41449 in China General Microbiological Culture Collection Center. The virus Vphv1-gamma can be secreted to outside of cells, therefore, culture liquid filtered off mycelium can be used as a biocontrol agent for prevention and treatment of pear tree and apple tree Valsa diseases, and can effectively weaken or inhibit pathogenicity and diffusion of Valsa pyri and Valsa mali, and has a prospect for being used for producing and practicing biological prevention and treatment of pear tree and apple tree Valsa diseases.

Disclosed are synthetic Marivirus eye satellite RNA molecules and satellite particles containing the same. Also disclosed are synthetic mateur virus satellite RNA molecules containing an internal heterologous RNA virus (HRV) amplicon. Also disclosed are methods of altering plant phenotypes, improving stress resistance in plants, and improving pest and pathogen resistance in plants using the Marivirus eye satellite RNA molecules and satellite particles containing the same.

The invention provides an application of trichostatin A in promotion of RNA virus proliferation, and belongs to the field of chemical medicines. The invention discloses a novel application of trichostatin A. The trichostatin A can effectively promote the-1 Ribosomal Frameshift process of RNA viruses, can effectively promote replication and proliferation of the RNA viruses, can be used for preparing products for promoting proliferation of the RNA viruses and can be used for producing and preparing RNA virus inactivated vaccines or attenuated vaccines, and the application of the trichostatin A in promoting proliferation of the RNA viruses and the application of the trichostatin A in promoting proliferation of the RNA viruses and the application of the trichostatin A in promoting proliferation of the RNA viruses and the application of the trichostatin A in promoting proliferation of the RNA viruses and the application of the trichostatin A in promoting proliferation of the RNA viruses and the application of the trichostatin A in promoting proliferation of the RNA viruses. And the production cost can be effectively reduced, the production period is shortened, and wide application prospects and economic values are achieved.

The invention discloses a detection reagent, a kit and a detection method for pathogens of micropterus salmoides. The micropterus salmoides pathogen detection reagent comprises primers and probes for specifically amplifying an infectious spleen and kidney necrosis virus, a viral nervous necrosis virus, flavobacterium columnar, a perch rhabdovirus, a micropterus salmoides double-RNA virus, edwardsiella tarda, nocardia and a micropterus salmoides iridovirus; specific upstream and downstream primers and probes of the eight pathogens are sequences as shown in SEQ ID NO.1 to SEQ ID NO.24 in sequence. The micropterus salmoides pathogen detection reagent and method can detect eight pathogens at the same time, have the advantages of high sensitivity, high specificity, good repeatability and the like, provide reliable basis for prevention and control of the pathogens, also improve the prevention and control work efficiency, and have good application prospects. The kit is especially suitable for large-scale rapid screening and mixed infection diagnosis of aquaculture pathogens, and has important value and significance for prevention and treatment of micropterus salmoides pathogens.

The invention discloses a rolling circle amplification (RCA) molecular beacon based on DNA synthesized silver nanoclusters (AgNCs / DNA) and application of the rolling circle amplification (RCA) molecular beacon in DNA and RNA virus detection. According to the invention, a padlock template probe PlP with a specific secondary structure is used for carrying out two-enzyme one-step method RCA to generate single-stranded DNA containing a G-rich sequence tandem repeat element. Then H2O2 is added to eliminate the influence of residual dithiothreitol DTT in the RCA reaction, and then AgNCs / DT-GSP-t4 is used as a signal probe to generate a specific fluorescence enhancement signal for a to-be-detected target under the action of a G-rich proximity fluorescence enhancement effect. The RCA reaction does not need primer mediation, so that PlP design and RCA operation steps are simplified; meanwhile, the single-PlP or double-PlP probe is combined with the fluorescent molecular beacon, so that single-base mismatch at a PlP connection point can be recognized, and the probe has the advantage of high specificity.

The application discloses an antiviral polypeptide targeting virus-host interaction and application, the polypeptide is composed of 1-17 amino acids of ARF1, a transmembrane peptide TAT and an intermediate connecting peptide, and the amino acid sequence is shown as SEQ ID NO:1; the polypeptide provides a new strategy for the prevention and control of RNA viruses, and also provides a new theoretical basis for accelerating the research and development of broad-spectrum antiviral polypeptide small molecule drugs.

A hybrid protein comprises a first domain comprising a sequence encoding a surface protein of an enveloped RNA virus and a second domain comprising a sequence encoding an ectodomain of a type 2 transmembrane domain protein, wherein the second domain is located at the C-terminal of the first domain. The hybrid protein or an mRNA encoding such protein can be used as a vaccine against the infection of the enveloped RNA virus.

The invention relates to a porcine reproductive and respiratory syndrome chimeric virus vaccine strain as well as a construction method and application thereof, and belongs to the technical field of biology. The porcine reproductive and respiratory syndrome chimeric virus provided by the invention is an RNA (Ribonucleic Acid) virus, and the nucleotide sequence of the RNA virus is SEQ ID NO: 1. The chimeric virus has better safety and effectiveness, can relieve lung injury caused by the porcine reproductive and respiratory syndrome virus and promote removal of viruses in piglets, can be used as a vaccine candidate strain of the porcine reproductive and respiratory syndrome virus, and is beneficial to prevention and control of the porcine reproductive and respiratory syndrome.

Synthetic Tombusviridae satellite RNA molecules and satellite particles containing the same are disclosed. Also disclosed are synthetic Tombusviridae satellite RNA molecules containing internal heterologous RNA virus (HRV) amplicons. Methods of using the Tombusviridae satellite RNA molecules and satellite particles containing the same to alter plant phenotype, improve stress resistance of plants, and improve pest and pathogen resistance of plants are also disclosed.

The invention discloses a nucleoside antiviral NHC diester prodrug as shown in a formula I and pharmaceutically acceptable salts thereof, and a preparation method and medical application of the nucleoside antiviral NHC diester prodrug. Pharmacological experiments prove that the compound disclosed by the invention has relatively strong inhibitory activity on replication of multiple RNA viruses. Particularly, the compound has good lung microsome release efficiency, the species difference of the lung release efficiency is small, the consistency of the in-vivo drug effect of the antiviral drug and the in-vivo drug effect of the antiviral drug can be improved, and the risk of species difference of prodrug release antiviral active ingredients is avoided. Therefore, the compound provided by the invention has good broad-spectrum antiviral activity, and can be used as a broad-spectrum antiviral drug for treating various respiratory virus infections.

This invention relates to the field of biotechnology, and more particularly to a mutant of the Rift Valley fever virus L protein and its applications. The amino acid sequence of the mutant is based on the amino acid sequence shown in SEQ ID NO:1, with mutations made at position 1189 (aspartic acid) and / or position 1205 (tryptophan). Through cryo-electron microscopy structural analysis and in vitro mismatch experiments, this invention has confirmed that both identified key amino acid sites are involved in regulating the synthetic fidelity of viral polymerase: mutation at N1189 decreases the synthetic fidelity of the polymerase, while mutation at W1205 increases it. This invention not only provides a new target for the development of attenuated vaccines but also provides crucial evidence for elucidating the molecular regulatory mechanisms of RNA virus fidelity.

The present disclosure provides a method of producing a negative-strand RNA virus vector. The present disclosure specifically provides a method of producing a negative-strand RNA virus vector in the presence of a PKR inhibitory factor.

The invention provides a multiplex RPA (recombinase polymerase amplification) detection primer probe group, a test strip, a kit and a detection method for three prawn RNA (ribonucleic acid) viruses, and belongs to the technical field of biological detection. The invention provides a multiple RPA (recombinase polymerase amplification) detection primer probe group for three prawn RNA (ribonucleic acid) viruses. The multiple RPA detection primer probe group is designed by taking an IMNV MCP gene, a CMNV RdRp gene and a PvPV RdRp gene as target genes. The primer probe set designed by the invention meets the requirements of multiple RPA detection, and can specifically amplify corresponding viral nucleic acid. The invention also provides multiple RPA (recombinase polymerase amplification) detection capable of completing the three RNA viruses of prawns. When the triple RPA detection method constructed by the invention is used for detecting IMNV and CMNV in a sample, the result is completely consistent with the result of an RT-qPCR detection method. The triple RPA detection method established by the invention can be used for simultaneously detecting three viruses in a shorter time, and has a remarkable on-site detection advantage.

Synthetic turnip yellow mosaic virus satellite RNA molecules and satellite particles containing the same are disclosed. Synthetic turnip yellow mosaic virus satellite RNA molecules containing an internal heterologous RNA virus (HRV) amplicon are also disclosed. Also disclosed are methods of altering plant phenotypes, improving stress resistance in plants, and improving pest and pathogen resistance in plants using turnip yellow mosaic virus satellite RNA molecules and satellite particles containing the same.

The invention provides a Vp8-Apaf1 fusion protein as well as a preparation method and application thereof, and relates to the technical field of biological medicines. According to the invention, a Vp8-Apaf1 fusion protein is constructed and expressed, the protein is formed by connecting a dsRBD binding domain of a Vp8 protein of a reoviridae Kadipiro virus and a signal transduction domain of human Apaf-1 composed of CARD, and the amino acid sequence of the fusion protein Vp8-Apaf1 is as shown in SEQ ID NO. 1. According to the preparation method provided by the invention, the Vp8-Apaf1 fusion protein can be successfully and stably expressed. The Vp8-Apaf1 fusion protein provided by the invention has an obvious inhibition effect on RNA (Ribonucleic Acid) virus VSV and DNA (Deoxyribose Nucleic Acid) virus HSV1, and the antiviral effect of the Vp8-Apaf1 fusion protein is influenced by the Vp8-Apaf1 fusion protein in a dose-dependent manner; the Vp8-Apaf1 fusion protein has relatively strong inhibition, prevention and killing effects on the VTT-GFP (Virus Triglyceride-Green Fluorescent Protein). The Vp8-Apaf1 fusion protein provided by the invention has a broad-spectrum antiviral effect, has relatively strong inhibiting, preventing and killing effects on viruses, and provides a new idea for developing broad-spectrum antiviral preparations.

The application discloses a kind of RNA virus nucleic acid preservative in fecal sample, including denaturant, chelating agent, pH buffering agent, osmotic pressure regulator and reducing agent;The denaturant includes guanidine salt and surfactant;The application further additionally provides a kind of RNA virus nucleic acid preservative in fecal sample preparation method, preparation above-mentioned one kind of RNA virus nucleic acid preservative in fecal sample, including the following steps: each component is mixed with ratio, pH is adjusted with pH buffering agent, with deionized water constant volume;Filter, obtain the preservative solution described in this application.The preservative solution of the application can stably store human cell DNA and viral RNA in fecal sample at room temperature for at least 7 days, which is beneficial for long-distance transportation at room temperature;Meanwhile, the preservative solution of the application also releases nucleic acid, inactivates virus, so as to prevent biological leakage and infection phenomenon;The de-inhibitor component in the preservative solution can directly combine and precipitate with various inhibitors in feces, reducing the interference to nucleic acid detection.

This invention provides the use of compounds in the preparation of drugs for the prevention or treatment of RNA virus infections, wherein the compounds are any one of the following groups: (1) L-2-aminoadipic acid or a pharmaceutical salt thereof; (2) L-lysine or a pharmaceutical salt thereof. The compounds L-2-aminoadipic acid or L-lysine can significantly inhibit the production of infectious RNA virus particles and inhibit RNA virus replication within a non-toxic range. L-2-aminoadipic acid or L-lysine, as essential metabolites for the human body, have higher safety, and are widely available, inexpensive, and easily obtained.