RNA virus inactivation preservation solution and application thereof

A technology of RNA virus and preservation solution, which is applied in the field of virus biological detection, can solve the problems of cumbersome virus decrosslinking process, inactivation of RNA virus, and operator hazards, so as to preserve the integrity of RNA, reduce the risk of infection, and save energy. the effect of time
CN111363729AActive Publication Date: 2020-07-03苏州白垩纪生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
苏州白垩纪生物科技有限公司
Publication Date
2020-07-03

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Abstract

The present invention discloses an RNA virus inactivation preservation solution. The inactivation preservation solution comprises ammonium salt, 1-ethyl-3-methylimidazolium bisulfate, lithium chloride, ethylenediaminetetraacetic acid, an RNase inhibitor, a surfactant, a reducing agent, a buffer agent and water. The present invention also provides an RNA virus sample inactivation and preservation method and an RNA virus detection method, and the method is conducted by using the inactivation preservation solution. The preservation solution has low cost, inhibits RNase activity, preserves RNA integrity, realizes preservation and transportation of samples at room temperature, can also quickly inactivate pathogens at room temperature, reduces infection risks of medical personnel, and reduces false negatives in detection. At the same time, the inactivation preservation solution can realize a lysis function, does not contain chaotropic reagents and alcohols, is compatible with mainstream nucleic acid extraction kits on the market, greatly saves time of an entire process, and is particularly suitable for 2019 new coronavirus (COVID-19) inactivation and lysis steps after swab sampling.
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Description

technical field

[0001] The invention relates to the technical field of biological detection of viruses, in particular to a preservation solution for inactivating an RNA virus and its application. Background technique

[0002] Regarding the new type of coronavirus (COVID-19, referred to as: new coronavirus) pneumonia, on the one hand, it is known that the epidemic prevention departments and hospitals regard the positive nucleic acid test of the new coronavirus as the gold standard for diagnosis; The PCR tests were all negative, and there was even a report that a case did not detect a positive result until the seventh test. Therefore, how to avoid the false negative rate of nucleic acid detection is the biggest dilemma encountered by current PCR detection.

[0003] The "Diagnosis and Treatment Plan for Novel Coronavirus Infected Pneumonia" has two criteria for the diagnosis of infection cases: 1) positive real-time fluorescence RT-PCR detection of new coronavirus nucleic acid...

Claims

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