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136results about How to "Reduce false negatives" patented technology

Transcriptome-based tumor neoantigen identification method

The invention discloses a transcriptome-based tumor antigen identification method. The method comprises four steps of: obtaining an RNA sample of a patient tumor tissue, and carrying out library construction and amplification on the RNA sample to obtain an RNA sample sequencing result of the tumor tissue; aligning short read segments of the RNA sample sequencing result to a human reference genometo obtain an RNA alignment result; calculating gene expression quantity according to the RNA alignment result, and carrying out mutation detection and prediction of fusion gene events according to theRNA alignment result; and predicting transcriptome HLA typing according to the alignment result, wherein calculation of the gene expression quantity, mutation detection and prediction of the fusion gene events are carried out according to a specified order or simultaneously carried out; and using the gene expression quantity of a transcriptome sample, depth of transcriptome mutation sites in a whole-exon sequencing sample and binding force of neonatal short peptides and the patient HLA typing as an analysis result to submit the same to a downstream analyst. The invention provides the method capable of identifying a tumor-specific antigen of an individual sample from tumor patient transcriptome NGS data.
Owner:HANGZHOU NEOANTIGEN THERAPEUTICS CO LTD

Pedestrian detection method and device

The embodiment of the invention provides a pedestrian detection method and device. The pedestrian detection method comprises the steps that an image to be processed is acquired; the pedestrians in theimage to be processed are detected so as to acquire the pedestrian information, wherein the pedestrian information includes multiple pedestrian boxes; the heads of the pedestrians in the image to beprocessed are detected so as to acquire the head information, wherein the heat information includes the head positions corresponding to at least a part of the pedestrian boxes of multiple pedestrian boxes in a one-to-one way; the shoulders of the pedestrians in the image to be processed are detected so as to acquire the shoulder information, wherein the shoulder information includes the left shoulder positions and the right shoulder positions which are corresponding to at least a part of the pedestrian boxes in a one-to-one way; the overlapping situation of the head and shoulder parts corresponding to at least a part of the pedestrian boxes in a one-to-one way is calculated according to the head positions and the left shoulder positions and the right shoulder positions which are corresponding to at least a part of the pedestrian boxes in a one-to-one way; and the pedestrian boxes are filtered according to the overlapping situation so as to acquire the pedestrian detection result. According to the method and the device, the problem that the pedestrian boxes of other pedestrians are filtered by using the pedestrian box of a certain pedestrian can be avoided.
Owner:BEIJING KUANGSHI TECH +1

Knowledge graph completion method based on neural network

The embodiment of the invention provides a knowledge graph completion method based on a neural network, and relates to the technical field of natural language processing. The method comprises the steps of S1, converting triples (s, r, o) in a knowledge graph K into sentences [s r o] in an embedding layer, and converting the sentences [s r o] into a k-dimensional dense vector representation form xi= [vs, vr, vo] by utilizing a vector representation technology; s2, learning long-term dependence of sentences from the input feature vector xi in a loop layer by utilizing a BiLSTM network to obtaina loop layer feature vector hRNN; s3, learning local structure information from the feature vector hRNN in a convolutional layer by using a convolutional neural network to obtain a convolutional layer feature vector hCNN; and S4, converting the convolutional layer feature vector hCNN into a score of each triple (s, r, o) in a full connection layer. According to the knowledge graph completion method, the long-term dependence and local structure information of facts in the knowledge graph are captured by using the recurrent and convolutional neural network without depending on any external data, and meanwhile, the transfer characteristics of entities and relationships are reserved, so that the learning ability is higher.
Owner:SOUTHWEST JIAOTONG UNIV

Digital PCR-based novel coronavirus nucleic acid quantitative detection kit and application

The invention discloses a digital PCR-based novel coronavirus nucleic acid quantitative detection kit and an application. The reaction total system of the digital PCR-based novel coronavirus nucleic acid quantitative detection kit has 20 ul, comprising 10ul of 2x One-Step RT-ddPCR Supermix, 0.8ul of 25mM manganese acetate solution, 5ul of to-be-detected sample RNA, 1ul of ORFlab gene primer probeworking solution, 1ul of N gene primer probe working solution, 1ul of RPP30 gene primer probe working solution and 1.2ul of Nuclease-Free Water, wherein 5ul of negative control RNA extraction solutionand 5ul of positive control RNA extraction solution are adopted to replace the to-be-detected sample RNA in a negative control reaction system and a positive control reaction system respectively. Based on the innovative RNA one-step reverse transcription microdroplet type digital PCR technology, nucleic acid absolute quantification is carried out specific to highly conservative ORFlab gene and Ngene in the novel coronavirus (2019-nCoV) genome, so that the detection accuracy is improved, and the kit can be used for clinical assisted diagnosis and viral load analysis of novel coronavirus (2019-nCoV) infection, and has a wide clinical application value.
Owner:南京实践医学检验有限公司

Automatic nucleic acid sampling and detecting equipment

The invention discloses automatic nucleic acid sampling and detecting equipment. The equipment comprises a detection cabin, and a sampling device, a detection device and a control system which are arranged in the detection cabin, wherein the detection cabin comprises a cabin body and a solar power generation system, and an entrance is formed in one side of the cabin body and provided with an intelligent door for opening or closing the entrance; the sampling device comprises a jaw bracket, a multi-degree-of-freedom mechanical arm and an image collector; and the detection device comprises a test tube conveying belt, a sorting mechanical arm, a constant-temperature box, a medicine injector and a color recognizer, wherein the sorting mechanical arm sorts nucleic acid test tubes into the constant-temperature box and sorts the nucleic acid test tubes to the position below the color recognizer for color recognition, and the color recognizer transmits detection information to the control system. The whole nucleic acid detection process does not need the operation of nurses, and the operations from nucleic acid detection, to appointment auditing, automatic sampling of pharyngeal specimens and finally nucleic acid detection result sending, are completed by corresponding devices, so that cross infection during manual detection is avoided.
Owner:CHINA UNIV OF GEOSCIENCES (WUHAN)

Multiple PCR detection kit for virulence factors of streptococcus suis and detection method thereof

The invention discloses a multiple PCR detection kit for virulence factors of streptococcus suis and a detection method thereof, and belongs to the technical field of biology. The multiple PCR detection kit comprises nucleic acid shown by base sequences, such as SEQ ID NO:1 to SEQ ID NO:20. The detection method for the multiple PCR detection kit comprises the following steps of: 1, providing DNA of samples to be detected; 2, taking the kit, amplifying the DNA of the samples to be detected by adopting the conventional PCR method, detecting an amplified result by adopting an agarose gel electrophoresis method, and judging according to the result; and by using positive DNA of streptococcus suis 2 type as contrast, if the contrast proves that not all the strips are amplified, re-detecting, and if the contrast proves that all the strips are amplified, judging the result. The multiple PCR detection method established by the invention is specific and sensitive, and has the advantages of simpleness, convenience and quickness. The multiple PCR detection kit has reliable stability, and can be used for rapid detection of clinical samples and survey research on molecular epidemiology for the streptococcus suis.
Owner:SHANGHAI JIAO TONG UNIV

Ramp speed limit recognition method and device

The invention discloses a ramp speed limit recognition method, which comprises steps: the speed limit sign information recognized from an acquired road image is obtained; based on the speed limit information, whether the speed limit information is ramp speed limit information is judged; if yes, the lane information, the route planning information, the traffic light information and the environmentinformation of the vehicle are obtained; according to the lane information, the route planning information, the traffic light information and the environment information, whether the vehicle enters aramp driving-in preparation state is judged; and if the vehicle is determined to enter the ramp driving-in preparation state, based on the ramp speed limit information, a speed limit value of the vehicle is determined and prompted. According to the method and the device provided in the invention, the correct recognition rate of the ramp speed limit recognition scheme can be improved, missed reportand error report risks can be reduced, and the driving experience of a driver can be improved. When the vehicle is overspeed after entering the ramp, the vehicle speed is limited below the ramp speedlimit, and the driving safety factor of the vehicle is improved.
Owner:福瑞泰克智能系统有限公司

Circulation tumor cell separation and enrichment micro-fluidic chip and enrichment method thereof

ActiveCN107402303AEfficient fully automatic separation and purificationFast automatic separation and purificationMaterial analysisEnrichment methodsMagnetic bead
The invention discloses a circulation tumor cell separation and enrichment micro-fluidic chip and an enrichment method thereof. The circulation tumor cell separation and enrichment micro-fluidic chip comprises a sample treatment chamber and a separation purification chamber, wherein the separation purification chamber comprises a micro channel, a magnetic field adsorption part, a magnetic collection chamber, a cell collection chamber and a filtering membrane; a sub-channel is formed inside the micro channel; the magnetic field adsorption part is arranged on the inner wall of the sub-channel; one end of the micro channel is communicated with the sample treatment chamber; the other end of the micro channel is communicated with the magnetic collection chamber and the cell collection chamber in sequence; the magnetic collection chamber is used for collecting magnetic beads which are not adsorbed by the inner wall of the sub-channel; the filtering membrane which is capable of retaining circulation tumor cells is arranged inside the cell collection chamber; and a liquid outlet from which a liquid filtered by the filtering membrane flows out is formed in the cell collection chamber. The circulation tumor cell separation and enrichment micro-fluidic chip is efficient, rapid, good in specificity and high in accuracy.
Owner:SUREXAM BIO TECH
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