76 results about "Mycobacterium tuberculosis" patented technology

The invention relates to the technical field of high-throughput targeted sequencing, in particular to a primer group for detecting important drug-resistant genes and virulence genes of multi-drug-resistant pathogenic bacteria and application of the primer group. Specifically, the kit comprises detection primers for multiple drug-resistant bacteria such as third-generation cephalosporin drug-resistant enterobacteriaceae bacteria, carbapenem drug-resistant enterobacteriaceae bacteria, carbapenem drug-resistant acinetobacter baumannii, rifampicin drug-resistant mycobacterium tuberculosis and the like. The method has the technical advantages of rapidness, high efficiency, strong targeting property, strong specificity and the like, is suitable for epidemic and difference analysis of various important drug-resistant genes and virulence genes carried by metagenome samples of various environments or disease materials and the like, evaluates the risk of related environments on the basis, and can further guide prevention and clinical treatment medication.

The invention discloses application of nadifloxacin, and relates to the technical field of biological medicines. According to the application disclosed by the invention, the condition that the nadifloxacin not only can better inhibit the Mycobacterium tuberculosis, but also can better inhibit the Mycobacterium nontuberculosis is found for the first time, so that the nadifloxacin can be used for preparing an inhibitor of the Mycobacterium tuberculosis and / or the Mycobacterium nontuberculosis; the invention also relates to a medicine for treating diseases caused by dry infection of Mycobacterium tuberculosis and / or non-Mycobacterium tuberculosis.

The invention discloses a mycobacterium tuberculosis tyrosine phenol lyase mutant and application thereof, and belongs to the field of molecular biology and the technical field of biological catalysis. The mutant is obtained by mutating 384 isoleucine of tyrosine phenol lyase from mycobacterium tuberculosis into valine, and is marked as a mutant I384V. The I384V and wild type tyrosine phenol lyase have similar optimum temperature and optimum pH, but the I384V shows higher specific enzyme activity and catalytic efficiency than the wild type tyrosine phenol lyase. Compared with a wild type, the tyrosine phenol lyase mutant I384V has the advantages that the synthesis efficiency of the tyrosine phenol lyase mutant I384V on the synthesis of levodopa is improved by 38.4%, and the tyrosine phenol lyase mutant I384V has wide prospects in large-scale industrial application.

The invention relates to the technical field of gene detection, and discloses a mycobacterium tuberculosis drug resistance gene detection data analysis system which comprises a data import module, a signal correction module, a feature extraction module, a multi-stage interpretation module, a result fusion module and a report generation module. The system performs baseline drift correction, abnormal point detection and nonlinear normalization on a fluorescence intensity value output by a detector, extracts multi-dimensional features such as peak intensity, a mutation ratio, a consistency coefficient and an in-batch difference index, and constructs a dynamic threshold function to realize wild type and mutation type layered judgment. A drug resistance risk conclusion is generated through drug resistance characteristic matrix mapping, a standardized detection report is output, and intelligent analysis of a detection result and automatic report generation are achieved. Through multi-stage signal correction, multi-dimensional feature extraction and dynamic threshold interpretation, intelligent analysis and standardized report output of a mycobacterium tuberculosis drug resistance gene detection result are realized, and data judgment accuracy and clinical application efficiency are improved.

The invention discloses an image processing-based tongue swab sample fluorescence detection method for Mycobacterium tuberculosis, and relates to the technical field of image processing, the image processing-based tongue swab sample fluorescence detection method comprises a flora monitoring module, an image analysis module and a concentration determination module, the flora monitoring module is used for identifying a flora culture solution and monitoring flora on the flora culture solution in real time, and the concentration determination module is used for determining the concentration of Mycobacterium tuberculosis; the image analysis module is used for carrying out straight division processing and flora contour fitting on a shot image, and the concentration determination module is used for analyzing the flora size, the shape and the life cycle of mycobacteria in image data to carry out targeted fluorescent agent concentration adjustment on florae with different growth conditions. The flora monitoring module comprises an image monitoring unit and a flora culture solution recognition module, the image monitoring unit is electrically connected with the flora culture solution recognition module and the flora recognition module, and the flora culture solution recognition module is electrically connected with the flora recognition module.

The present invention relates to specific binding molecules which bind to the HLA-E restricted peptide RLPAKAPLL (SEQ ID NO: 1) derived from Mycobacterium tuberculosis enoyl-ACP reductase. Said specific binding molecules may comprise CDR sequences embedded within a framework sequence. The CDRs and framework sequences may correspond to a T cell receptor (TCR) variable domain and may further comprise non-natural mutations relative to a native TCR variable domain. The specific binding molecules of the invention are particularly suitable for use as novel immunotherapeutic reagents for the treatment of infectious disease.

The invention belongs to the technical field of biology, and relates to a pathogen detection technology based on a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, in particular to a tubercle bacillus detection and activity determination method based on fluorescent T7-CRISPR. The first purpose is to provide a reagent combination for detecting viable tubercle bacillus, and the reagent combination comprises a) a T7 reverse transcription reaction reagent and b) a CRISPR-Cas13a reaction system; a) the T7 reverse transcription reaction reagent comprises a T7 reverse transcription primer and a T7 reverse transcriptase; and b) the CRISPR-Cas13a reaction system comprises specific crRNA, a fluorescence labeled ssRNA reporter, a reaction buffer solution, a Cas13a protein, RNase-Free H2O and a T7 reverse transcriptase. The invention also provides application of the reagent combination in detection of viable tubercle bacillus. The primer combination disclosed by the invention has the advantages of strong specificity and high sensitivity in detection application, can realize rapid detection and visual detection, and is low in detection cost, simple and convenient to operate and suitable for large-scale clinical application.

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a deadly global disease. Embodiments of the invention comprise an improved vaccine for generating an immune response and preventing or treating mycobacterial diseases such as tuberculosis in humans and animals. Embodiments of the invention also comprise a method for using the vaccine against such mycobacterial diseases.

The utility model relates to the technical field of interpretation training, and discloses a tubercle bacillus skin test result interpretation training device which comprises a three-dimensional optical measuring instrument, a carrier plate is placed in the middle of the inner side of the bottom wall of the three-dimensional optical measuring instrument, an operation table is placed on one side of the three-dimensional optical measuring instrument, and an arm model is placed on the operation table. According to the utility model, through the arrangement of the arm model, the induration model and the reddish halo model, in the use process of the tubercle bacillus skin test result interpretation training device, a student can hold the arm model to observe the peripheral convex model, and a practical operator identifies the induration part and measures the induration reading by using the measuring scale, so that the induration judgment and reading are realized; then, teaching personnel detach the induration model, the induration model is clamped on the upper side of the carrier plate through the strip-shaped opening and located on the right lower side of the measuring head, corresponding size values are obtained through the display screen, whether manual measurement is accurate or not is known, and the structure can train the recognition ability of students and exercise the measurement ability.

The utility model relates to the technical field of sample detection, in particular to a tubercle bacillus drug resistance comparison detection device which comprises a bottom plate, a control panel fixedly connected to the front side of the bottom plate, a first sliding groove formed in the top end of the bottom plate, a fixed base arranged at the top end of the bottom plate, a culture dish in contact with the top end of the fixed base and a clamping block slidably connected to the fixed base. The electric push rod is controlled to be started by operating the control panel, the sliding block is driven to move on the inner side of the first sliding groove through stretching of the sliding rod of the electric push rod, and the electric push rod is driven to move on the inner side of the first sliding groove. The slide block moves to drive the culture dish arranged at the top end of the fixed base fixed by the slide block to move, and the moved culture dish is consistent with the previous culture dish in position, so that a user can directly observe the difference between different culture dishes, and the time consumed for adjusting the microscope is shortened.

The invention discloses a detection kit based on orthogonal dual-channel label-free CRISPR-Cas and application of the detection kit, relates to the technical field of nucleic acid detection, and constructs an orthogonal dual-channel response system by utilizing the differentiated cleavage activity of Cas12a targeted DNA and Cas13 targeted RNA to a substrate. A Cas12a pathway is reported by utilizing protoporphyrin IX to be compounded with G-quadruplex G4DNA, and a Cas13a pathway is reported by utilizing DFHBI to be compounded with a Broccoli RNA structure, so that label-free signal transduction is realized. Through accurate screening of fluorophores, channel specificity is ensured, and optical signal crosstalk is eliminated. Mycobacterium tuberculosis (MTB) and respiratory syncytial virus (RSV) are used as model pathogens for verification, and a multiple recombinase polymerase amplification (RPA) technology is combined, so that the detection sensitivity on a synthetic target reaches a single molecule level. The system has high specificity, and even if the concentration of a non-target pathogen is increased by 100 times, no cross reaction exists.

A molecular marker for drug resistance in Mycobacterium tuberculosis, a reagent kit for detecting drug resistance in Mycobacterium tuberculosis, and a detection method thereof, comprising detecting nonsynonymous mutations at specific gene loci in the pncA gene, rpsA gene, or panD gene, or detecting mutations at specific amino acid loci in the pncA protein, rpsA protein, or panD protein, can help doctors select the most suitable treatment plan for pulmonary tuberculosis, improve the success rate of treatment, reduce the infection and spread of pulmonary tuberculosis, thereby protecting patients and public health.

A polypeptide with an anti-Mycobacterium tuberculosis activity, and a preparation method and use thereof are provided. The preparation method of the polypeptide includes the following steps: (1) preparing a straight-chain polypeptide with a sequence shown in SEQ ID NO: 1; and (2) allowing sulfhydryl groups of two cysteine residues in the straight-chain polypeptide to undergo a dehydration-condensation reaction with dimethylolurea to obtain the polypeptide. The polypeptide has little cytotoxicity and a prominent anti-Mycobacterium tuberculosis effect.

This invention discloses a mutant of Mycobacterium tuberculosis tyrosine lyase and its applications, belonging to the fields of molecular biology and biocatalysis. This mutant, denoted as mutant I384V, is obtained by mutating isoleucine at position 384 of the Mycobacterium tuberculosis tyrosine lyase to valine. I384V has similar optimal temperature and pH to the wild-type tyrosine lyase, but exhibits higher specific enzyme activity and catalytic efficiency. Compared to the wild-type, the mutant I384V improves the synthesis efficiency of levodopa by 38.4%, showing broad prospects for large-scale industrial application.

The application discloses a kind of absolute quantitative detection method of child respiratory tract multi-pathogen based on multi-target ladder microarray and exogenous internal reference correction.The method sets up independent micro-reaction unit to different pathogen, adds fixed amount of exogenous internal reference nucleic acid, and calculates template number by positive unit proportion combined with Poisson distribution model, then corrects the result by using the theoretical addition amount of exogenous internal reference, so as to realize parallel detection and absolute quantification of pathogen such as bocavirus, adenovirus, mycobacterium tuberculosis and mycoplasma pneumoniae without standard curve and special digital PCR equipment.The application has the advantages of high sensitivity, strong anti-interference ability, suitable for mixed infection sample analysis and clinical application, etc.

The invention relates to a method and a kit for detecting a rifampicin drug resistance gene of mycobacterium tuberculosis. The kit comprises polynucleotide primers as shown in SEQ ID NO.1-SEQ ID NO.7. The detection method comprises the following steps: step 1, collecting a sample: collecting a sputum sample of a patient as a test sample; step 2, extracting DNA: extracting DNA of a test sample by using a kit method or a lysis method; 3, preparing a reaction system, and carrying out reaction amplification, namely preparing an amplification reaction system under the guidance of the special primer for the kit by taking the sample DNA in the step 2 as a template, and carrying out specific amplification; step 4, result analysis: in the specific amplification process, due to the difference of amplification target sequences, the basic group contents of amplification products are different; the change of a single basic group can cause the change of the melting temperature of amplified target double chains; the method comprises the following steps: monitoring the change of a fluorescence signal in a temperature rise process in real time, performing data integration and image drawing on a detection result to generate a target product melting curve, and judging the difference of a gene sequence in a target product according to the difference of the melting curve.

The present invention relates to a multiplex gene detection system for viral and bacterial infection of the central nervous system, comprising forward and reverse primers for detecting enterovirus, human double echovirus, Mycobacterium tuberculosis, Listeria monocytogenes, Streptococcus pneumoniae, varicella zoster virus, mumps virus, cytomegalovirus, herpes simplex virus type 1, herpes simplex virus type 2, Neisseria meningitidis, Epstein-Barr virus, Streptococcus agalactiae, Haemophilus influenzae, human herpes virus type 6, Escherichia coli K1 and Japanese encephalitis virus, and the detection sample is cerebrospinal fluid. The multiplex gene detection product for viral and bacterial infection of the central nervous system of the present invention has high throughput, low cost, high sensitivity and good specificity, and is used for synchronous identification and semi-quantitative analysis of multiple viral and bacterial infections of the central nervous system.

The application discloses a mycobacterium membrane protein MmpL3 inhibitor, a pharmaceutical composition thereof and application. The application provides a compound as shown in formula I, a pharmaceutically acceptable salt or a stereoisomer thereof. The compound of the application has a good inhibiting effect on Mycobacterium tuberculosis, and has a good application prospect in treating various diseases related to mycobacterium.

An adenoviral vector with E1 and E3 regions removed and expressing the nucleoprotein or other immunogenic domain(s) of an influenza virus with or without the presence of the Autophagy-Inducing Peptide C5 (AIP-C5) from the CFP10 protein of Mycobacterium tuberculosis, compositions comprising same, and methods of use for general vaccination against heterosubtypic influenza viruses.

The invention discloses a deuterated tetracyclic derivative and application of the deuterated tetracyclic derivative in prevention or treatment of diseases caused by bacterial infection, and particularly application of the deuterated tetracyclic derivative in prevention or treatment of lung diseases caused by mycobacterium tuberculosis or drug-resistant mycobacterium tuberculosis.

The invention discloses a Mycobacterium tuberculosis double-target joint detection kit and a detection method, IS6110 and mpt64 genes are screened and determined as target genes for double-target joint detection of Mycobacterium tuberculosis, the detection false negative rate is reduced to a great extent, and the detection precision is improved. According to the invention, two pairs of specific primers and two probes are designed and synthesized, efficient, rapid and accurate double-target joint detection of mycobacterium tuberculosis is realized, the sensitivity and the specificity both reach 95% or more, and the matching degree of the two probes for joint detection of mycobacterium tuberculosis exceeds 90%. A unique reaction system is designed to carry out real-time fluorescent quantitative PCR, primer probes do not interfere with one another, amplification detection can be carried out at the same time, and high sensitivity and specificity are achieved. The sample does not need to be cultured, and the mycobacterium tuberculosis can be rapidly and accurately detected through sputum, so that the quantitative and qualitative detection of the mycobacterium tuberculosis is realized.

This invention relates to the field of high-throughput targeted sequencing technology, and particularly to a primer set and its applications for detecting important drug resistance and virulence genes in multidrug-resistant pathogens. Specifically, it includes primers for detecting various drug-resistant bacteria, such as third-generation cephalosporin-resistant Enterobacteriaceae, carbapenem-resistant Enterobacteriaceae, carbapenem-resistant Acinetobacter baumannii, and rifampicin-resistant Mycobacterium tuberculosis. This invention offers advantages such as speed, efficiency, strong targeting, and high specificity. It is suitable for analyzing the prevalence and differential distribution of multiple important drug resistance and virulence genes carried in metagenomic samples from various environments or pathogenic materials, and can be used to assess the risk of related environments, further guiding preventative and clinical treatment medications.

The invention belongs to the technical field of biology, and provides a mycobacterium tuberculosis detection method and a kit, the detection method comprises the following steps: adding a to-be-detected sample containing mycobacterium tuberculosis into a lysis working solution, carrying out a heat preservation reaction, centrifuging, and taking a supernatant, the cracking working solution contains a biotinylated metal complexing agent and biotinylated nanoparticles, and guanidine isothiocyanate and hydrophobic alkyl groups are connected to the biotinylated nanoparticles; adding a magnetic bead suspension into the supernatant, stirring at room temperature for reaction, standing, taking an upper solution, adding the upper solution into a purification column, purifying and washing to obtain a detection sample, and the surface of a magnetic bead contains streptavidin; and adding a detection sample into an amplification reagent for fluorescent PCR amplification, and analyzing a PCR amplification product by using a fluorescence melting curve to finish detection. The method can effectively reduce impurities contained in the detection sample and improve the detection accuracy.

The invention belongs to the technical field of biomedical detection and immunoassay, and particularly discloses a mycobacterium tuberculosis detection probe as well as a preparation method and application thereof. The mycobacterium tuberculosis detection probe provided by the invention comprises a capture probe and a signal probe, the capture probe is magnetic nanoparticles of which the surfaces are fixedly loaded with a mycobacterium tuberculosis specific antibody 1, and the signal probe is a mycobacterium tuberculosis specific antibody 2 of which the surface is marked with a photosensitizer. According to the mycobacterium tuberculosis detection probe disclosed by the invention, a photosensitizer labeling technology is combined with immunoassay, so that high-sensitivity detection of mycobacterium tuberculosis can be realized under a mild condition; under the irradiation of visible light, active oxygen generated by the photosensitizer can catalyze oxidation reaction of various chromogenic substrates, so that rapid colorimetric detection of mycobacterium tuberculosis is realized, and the kit is particularly suitable for the requirements of clinical serum sample detection and the like; in addition, the fluorescent probe also has the advantages of simple preparation process, strong recognition specificity, high detection sensitivity, good stability and reproducibility and the like.

To provide a useful method for detecting the genus Mycobacterium acid-fast bacterium species.SOLUTION: A method for detecting the genus Mycobacterium acid-fast bacterium species that can be included in a sample includes amplifying a plurality of nucleic acid regions derived from a multicopy gene of the genus Mycobacterium acid-fast bacterium species, and the multicopy gene has the following characteristics (a) and / or (b): (a) the sequence identity of the genus Mycobacterium acid-fast bacterium species of a detection target between each strain is 82% or more, and the sequence identity between the genus Mycobacterium acid-fast bacterium species of a detection target and the other genus Mycobacterium acid-fast bacterium species is less than 82%; and (b) a multicopy gene is present in more than 98% of strains of the genus Mycobacterium acid-fast bacterium species of a detection target.SELECTED DRAWING: None

The utility model relates to the technical field of medical treatment, and discloses a tubercle bacillus droplet collecting device which comprises a mask body, a one-way air outlet valve and ear hooks, the one-way air outlet valve is installed at the position, corresponding to the mouth and the nose, of the mask body, the ear hooks are connected to the two edge sides of the mask body, and the tubercle bacillus droplet collecting device further comprises a droplet collecting mechanism. The foam collecting mechanism comprises a mounting frame and a collecting assembly, the mounting frame is mounted at the position, corresponding to the one-way air outlet valve, of the inner side of the mask body, one side of the mounting frame penetrates out of the mask body, and the end face of the end, located outside the mask body, of the mounting frame is provided with a sliding cavity which extends in the length direction of the mounting frame and is open towards the side of the end face. The collecting assembly is slidably arranged in the concave cavity in the length direction of the mounting frame and used for collecting spittle of a patient. The utility model solves the problems in the prior art that the diagnosis rate of early-stage tuberculosis is insufficient and the etiological positive rate is very low.

The invention relates to the technical field of micro-fluidic electrochemical detection, in particular to a multi-channel micro-fluidic chip for detecting various drug-resistant mycobacterium tuberculosis as well as a use method and application of the multi-channel micro-fluidic chip. The multi-channel micro-fluidic chip comprises a splitting area and a sample mixing area, the splitting area comprises a sample injection hole and a DNA splitting chamber which are communicated in sequence, the sample mixing area comprises a sample mixing chamber, and the DNA splitting chamber and the sample mixing chamber are communicated through a first micro-channel; the electrode array area comprises a plurality of reaction detection chambers and detection electrodes, the detection electrodes are in one-to-one correspondence with the reaction detection chambers and are in contact with the inner spaces of the reaction detection chambers, the electrodes are modified with signal amplification probes, Cas12a-crRNA compounds are preloaded on the surfaces of the electrodes, and the reaction detection chambers are communicated with the sample mixing chamber through a second micro-channel. Multiple channels are designed, synchronous detection of multiple drug-resistant genes is achieved, integration of clinical sample treatment, DNA acquisition, DNA thermal denaturation treatment and DNA detection is achieved, operation is easy and smooth, and clinical diagnosis and medication guidance of tuberculosis drug resistance can be well served.

The present invention relates to a SNP molecular marker, a detection primer set, a detection kit and an application thereof; the present invention provides a SNP molecular marker associated with susceptibility to non-tuberculous mycobacteria, comprising: a SNP site with an RS number of rs194800, or / and a SNP site with an RS number of rs2520016; the SNP molecular marker of the present invention can be used to detect susceptibility to non-tuberculous mycobacteria, and provides a new solution for evaluating an individual's early warning risk of developing non-tuberculous mycobacteria.

This invention discloses a method for detecting tuberculous and non-tuberculous mycobacteria and its application, belonging to the field of biodetection technology. Addressing the combined problems of poor specificity, limited detection targets, high equipment costs, and long detection cycles in existing tuberculous and non-tuberculous mycobacteria detection technologies, this invention achieves specific detection by utilizing the ligation specificity of ligases. Ligases are highly precise; if a single base cannot be correctly matched, the ligation reaction will fail.

The invention discloses a sputum sample fluorescence microscopic image mycobacterium tuberculosis identification method, which comprises the following steps: carrying out multi-focal plane scanning on a sputum sample by adopting an optical microscope to obtain a continuous fluorescence slice image sequence; processing the fluorescence slice image sequence through a three-dimensional volume reconstruction algorithm to generate three-dimensional fluorescence volume data containing space coordinates and fluorescence intensity information; a thallus structure in the three-dimensional fluorescence volume data is converted into a graph model, nodes correspond to thallus feature points and carry space coordinates and fluorescence parameters, and edges are connected with adjacent feature points and reflect a space relation; according to the method, the recognition precision is high, the three-dimensional imaging and graph neural network technology is adopted, the multi-dimensional information of the thalli can be comprehensively obtained, the three-dimensional model is constructed and converted into the graph model for feature analysis, the problem of limitation of a two-dimensional view angle is effectively solved, and the recognition accuracy is high. And the identification accuracy of the mycobacterium tuberculosis is greatly improved.