29 results about "Mycobacterium tuberculosis" patented technology

The invention relates to the technical field of gene detection, and discloses a mycobacterium tuberculosis drug resistance gene detection data analysis system which comprises a data import module, a signal correction module, a feature extraction module, a multi-stage interpretation module, a result fusion module and a report generation module. The system performs baseline drift correction, abnormal point detection and nonlinear normalization on a fluorescence intensity value output by a detector, extracts multi-dimensional features such as peak intensity, a mutation ratio, a consistency coefficient and an in-batch difference index, and constructs a dynamic threshold function to realize wild type and mutation type layered judgment. A drug resistance risk conclusion is generated through drug resistance characteristic matrix mapping, a standardized detection report is output, and intelligent analysis of a detection result and automatic report generation are achieved. Through multi-stage signal correction, multi-dimensional feature extraction and dynamic threshold interpretation, intelligent analysis and standardized report output of a mycobacterium tuberculosis drug resistance gene detection result are realized, and data judgment accuracy and clinical application efficiency are improved.

The present invention relates to specific binding molecules which bind to the HLA-E restricted peptide RLPAKAPLL (SEQ ID NO: 1) derived from Mycobacterium tuberculosis enoyl-ACP reductase. Said specific binding molecules may comprise CDR sequences embedded within a framework sequence. The CDRs and framework sequences may correspond to a T cell receptor (TCR) variable domain and may further comprise non-natural mutations relative to a native TCR variable domain. The specific binding molecules of the invention are particularly suitable for use as novel immunotherapeutic reagents for the treatment of infectious disease.

The invention belongs to the technical field of biology, and relates to a pathogen detection technology based on a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, in particular to a tubercle bacillus detection and activity determination method based on fluorescent T7-CRISPR. The first purpose is to provide a reagent combination for detecting viable tubercle bacillus, and the reagent combination comprises a) a T7 reverse transcription reaction reagent and b) a CRISPR-Cas13a reaction system; a) the T7 reverse transcription reaction reagent comprises a T7 reverse transcription primer and a T7 reverse transcriptase; and b) the CRISPR-Cas13a reaction system comprises specific crRNA, a fluorescence labeled ssRNA reporter, a reaction buffer solution, a Cas13a protein, RNase-Free H2O and a T7 reverse transcriptase. The invention also provides application of the reagent combination in detection of viable tubercle bacillus. The primer combination disclosed by the invention has the advantages of strong specificity and high sensitivity in detection application, can realize rapid detection and visual detection, and is low in detection cost, simple and convenient to operate and suitable for large-scale clinical application.

The utility model relates to the technical field of sample detection, in particular to a tubercle bacillus drug resistance comparison detection device which comprises a bottom plate, a control panel fixedly connected to the front side of the bottom plate, a first sliding groove formed in the top end of the bottom plate, a fixed base arranged at the top end of the bottom plate, a culture dish in contact with the top end of the fixed base and a clamping block slidably connected to the fixed base. The electric push rod is controlled to be started by operating the control panel, the sliding block is driven to move on the inner side of the first sliding groove through stretching of the sliding rod of the electric push rod, and the electric push rod is driven to move on the inner side of the first sliding groove. The slide block moves to drive the culture dish arranged at the top end of the fixed base fixed by the slide block to move, and the moved culture dish is consistent with the previous culture dish in position, so that a user can directly observe the difference between different culture dishes, and the time consumed for adjusting the microscope is shortened.

The invention discloses a detection kit based on orthogonal dual-channel label-free CRISPR-Cas and application of the detection kit, relates to the technical field of nucleic acid detection, and constructs an orthogonal dual-channel response system by utilizing the differentiated cleavage activity of Cas12a targeted DNA and Cas13 targeted RNA to a substrate. A Cas12a pathway is reported by utilizing protoporphyrin IX to be compounded with G-quadruplex G4DNA, and a Cas13a pathway is reported by utilizing DFHBI to be compounded with a Broccoli RNA structure, so that label-free signal transduction is realized. Through accurate screening of fluorophores, channel specificity is ensured, and optical signal crosstalk is eliminated. Mycobacterium tuberculosis (MTB) and respiratory syncytial virus (RSV) are used as model pathogens for verification, and a multiple recombinase polymerase amplification (RPA) technology is combined, so that the detection sensitivity on a synthetic target reaches a single molecule level. The system has high specificity, and even if the concentration of a non-target pathogen is increased by 100 times, no cross reaction exists.

A molecular marker for drug resistance in Mycobacterium tuberculosis, a reagent kit for detecting drug resistance in Mycobacterium tuberculosis, and a detection method thereof, comprising detecting nonsynonymous mutations at specific gene loci in the pncA gene, rpsA gene, or panD gene, or detecting mutations at specific amino acid loci in the pncA protein, rpsA protein, or panD protein, can help doctors select the most suitable treatment plan for pulmonary tuberculosis, improve the success rate of treatment, reduce the infection and spread of pulmonary tuberculosis, thereby protecting patients and public health.

This invention discloses a mutant of Mycobacterium tuberculosis tyrosine lyase and its applications, belonging to the fields of molecular biology and biocatalysis. This mutant, denoted as mutant I384V, is obtained by mutating isoleucine at position 384 of the Mycobacterium tuberculosis tyrosine lyase to valine. I384V has similar optimal temperature and pH to the wild-type tyrosine lyase, but exhibits higher specific enzyme activity and catalytic efficiency. Compared to the wild-type, the mutant I384V improves the synthesis efficiency of levodopa by 38.4%, showing broad prospects for large-scale industrial application.

The application discloses a kind of absolute quantitative detection method of child respiratory tract multi-pathogen based on multi-target ladder microarray and exogenous internal reference correction.The method sets up independent micro-reaction unit to different pathogen, adds fixed amount of exogenous internal reference nucleic acid, and calculates template number by positive unit proportion combined with Poisson distribution model, then corrects the result by using the theoretical addition amount of exogenous internal reference, so as to realize parallel detection and absolute quantification of pathogen such as bocavirus, adenovirus, mycobacterium tuberculosis and mycoplasma pneumoniae without standard curve and special digital PCR equipment.The application has the advantages of high sensitivity, strong anti-interference ability, suitable for mixed infection sample analysis and clinical application, etc.

The invention relates to a method and a kit for detecting a rifampicin drug resistance gene of mycobacterium tuberculosis. The kit comprises polynucleotide primers as shown in SEQ ID NO.1-SEQ ID NO.7. The detection method comprises the following steps: step 1, collecting a sample: collecting a sputum sample of a patient as a test sample; step 2, extracting DNA: extracting DNA of a test sample by using a kit method or a lysis method; 3, preparing a reaction system, and carrying out reaction amplification, namely preparing an amplification reaction system under the guidance of the special primer for the kit by taking the sample DNA in the step 2 as a template, and carrying out specific amplification; step 4, result analysis: in the specific amplification process, due to the difference of amplification target sequences, the basic group contents of amplification products are different; the change of a single basic group can cause the change of the melting temperature of amplified target double chains; the method comprises the following steps: monitoring the change of a fluorescence signal in a temperature rise process in real time, performing data integration and image drawing on a detection result to generate a target product melting curve, and judging the difference of a gene sequence in a target product according to the difference of the melting curve.

The application discloses a mycobacterium membrane protein MmpL3 inhibitor, a pharmaceutical composition thereof and application. The application provides a compound as shown in formula I, a pharmaceutically acceptable salt or a stereoisomer thereof. The compound of the application has a good inhibiting effect on Mycobacterium tuberculosis, and has a good application prospect in treating various diseases related to mycobacterium.

This invention relates to the field of high-throughput targeted sequencing technology, and particularly to a primer set and its applications for detecting important drug resistance and virulence genes in multidrug-resistant pathogens. Specifically, it includes primers for detecting various drug-resistant bacteria, such as third-generation cephalosporin-resistant Enterobacteriaceae, carbapenem-resistant Enterobacteriaceae, carbapenem-resistant Acinetobacter baumannii, and rifampicin-resistant Mycobacterium tuberculosis. This invention offers advantages such as speed, efficiency, strong targeting, and high specificity. It is suitable for analyzing the prevalence and differential distribution of multiple important drug resistance and virulence genes carried in metagenomic samples from various environments or pathogenic materials, and can be used to assess the risk of related environments, further guiding preventative and clinical treatment medications.

The invention belongs to the technical field of biology, and provides a mycobacterium tuberculosis detection method and a kit, the detection method comprises the following steps: adding a to-be-detected sample containing mycobacterium tuberculosis into a lysis working solution, carrying out a heat preservation reaction, centrifuging, and taking a supernatant, the cracking working solution contains a biotinylated metal complexing agent and biotinylated nanoparticles, and guanidine isothiocyanate and hydrophobic alkyl groups are connected to the biotinylated nanoparticles; adding a magnetic bead suspension into the supernatant, stirring at room temperature for reaction, standing, taking an upper solution, adding the upper solution into a purification column, purifying and washing to obtain a detection sample, and the surface of a magnetic bead contains streptavidin; and adding a detection sample into an amplification reagent for fluorescent PCR amplification, and analyzing a PCR amplification product by using a fluorescence melting curve to finish detection. The method can effectively reduce impurities contained in the detection sample and improve the detection accuracy.

The invention belongs to the technical field of biomedical detection and immunoassay, and particularly discloses a mycobacterium tuberculosis detection probe as well as a preparation method and application thereof. The mycobacterium tuberculosis detection probe provided by the invention comprises a capture probe and a signal probe, the capture probe is magnetic nanoparticles of which the surfaces are fixedly loaded with a mycobacterium tuberculosis specific antibody 1, and the signal probe is a mycobacterium tuberculosis specific antibody 2 of which the surface is marked with a photosensitizer. According to the mycobacterium tuberculosis detection probe disclosed by the invention, a photosensitizer labeling technology is combined with immunoassay, so that high-sensitivity detection of mycobacterium tuberculosis can be realized under a mild condition; under the irradiation of visible light, active oxygen generated by the photosensitizer can catalyze oxidation reaction of various chromogenic substrates, so that rapid colorimetric detection of mycobacterium tuberculosis is realized, and the kit is particularly suitable for the requirements of clinical serum sample detection and the like; in addition, the fluorescent probe also has the advantages of simple preparation process, strong recognition specificity, high detection sensitivity, good stability and reproducibility and the like.

To provide a useful method for detecting the genus Mycobacterium acid-fast bacterium species.SOLUTION: A method for detecting the genus Mycobacterium acid-fast bacterium species that can be included in a sample includes amplifying a plurality of nucleic acid regions derived from a multicopy gene of the genus Mycobacterium acid-fast bacterium species, and the multicopy gene has the following characteristics (a) and / or (b): (a) the sequence identity of the genus Mycobacterium acid-fast bacterium species of a detection target between each strain is 82% or more, and the sequence identity between the genus Mycobacterium acid-fast bacterium species of a detection target and the other genus Mycobacterium acid-fast bacterium species is less than 82%; and (b) a multicopy gene is present in more than 98% of strains of the genus Mycobacterium acid-fast bacterium species of a detection target.SELECTED DRAWING: None

The invention relates to the technical field of micro-fluidic electrochemical detection, in particular to a multi-channel micro-fluidic chip for detecting various drug-resistant mycobacterium tuberculosis as well as a use method and application of the multi-channel micro-fluidic chip. The multi-channel micro-fluidic chip comprises a splitting area and a sample mixing area, the splitting area comprises a sample injection hole and a DNA splitting chamber which are communicated in sequence, the sample mixing area comprises a sample mixing chamber, and the DNA splitting chamber and the sample mixing chamber are communicated through a first micro-channel; the electrode array area comprises a plurality of reaction detection chambers and detection electrodes, the detection electrodes are in one-to-one correspondence with the reaction detection chambers and are in contact with the inner spaces of the reaction detection chambers, the electrodes are modified with signal amplification probes, Cas12a-crRNA compounds are preloaded on the surfaces of the electrodes, and the reaction detection chambers are communicated with the sample mixing chamber through a second micro-channel. Multiple channels are designed, synchronous detection of multiple drug-resistant genes is achieved, integration of clinical sample treatment, DNA acquisition, DNA thermal denaturation treatment and DNA detection is achieved, operation is easy and smooth, and clinical diagnosis and medication guidance of tuberculosis drug resistance can be well served.

The present invention relates to a SNP molecular marker, a detection primer set, a detection kit and an application thereof; the present invention provides a SNP molecular marker associated with susceptibility to non-tuberculous mycobacteria, comprising: a SNP site with an RS number of rs194800, or / and a SNP site with an RS number of rs2520016; the SNP molecular marker of the present invention can be used to detect susceptibility to non-tuberculous mycobacteria, and provides a new solution for evaluating an individual's early warning risk of developing non-tuberculous mycobacteria.

This invention discloses a method for detecting tuberculous and non-tuberculous mycobacteria and its application, belonging to the field of biodetection technology. Addressing the combined problems of poor specificity, limited detection targets, high equipment costs, and long detection cycles in existing tuberculous and non-tuberculous mycobacteria detection technologies, this invention achieves specific detection by utilizing the ligation specificity of ligases. Ligases are highly precise; if a single base cannot be correctly matched, the ligation reaction will fail.

The invention discloses a sputum sample fluorescence microscopic image mycobacterium tuberculosis identification method, which comprises the following steps: carrying out multi-focal plane scanning on a sputum sample by adopting an optical microscope to obtain a continuous fluorescence slice image sequence; processing the fluorescence slice image sequence through a three-dimensional volume reconstruction algorithm to generate three-dimensional fluorescence volume data containing space coordinates and fluorescence intensity information; a thallus structure in the three-dimensional fluorescence volume data is converted into a graph model, nodes correspond to thallus feature points and carry space coordinates and fluorescence parameters, and edges are connected with adjacent feature points and reflect a space relation; according to the method, the recognition precision is high, the three-dimensional imaging and graph neural network technology is adopted, the multi-dimensional information of the thalli can be comprehensively obtained, the three-dimensional model is constructed and converted into the graph model for feature analysis, the problem of limitation of a two-dimensional view angle is effectively solved, and the recognition accuracy is high. And the identification accuracy of the mycobacterium tuberculosis is greatly improved.

This invention discloses the application of a thermostable antigen of Mycobacterium tuberculosis, belonging to the field of biomedical technology. This invention provides an application of the thermostable antigen of Mycobacterium tuberculosis (Mtb-HAg). Immunization of mice with Mtb-HAg alone and in combination with BCG showed that immunization with Mtb-HAg alone induced a significantly higher specific immune response than with BCG and significantly stimulated lymphocyte proliferation. Immunization with Mtb-HAg in combination with BCG induced a stronger specific Th1 immune response, thereby significantly improving the immunogenicity of BCG, and demonstrating the potential of Mtb-HAg as a candidate subunit vaccine.

The invention relates to a cyanine compound and application thereof in fluorescence labeling of mycobacterium tuberculosis. The cyanine compound is a compound as shown in I, a tautomer or a pharmaceutically acceptable salt thereof. The cyanine compound provided by the invention can effectively mark Mtb, so that the cyanine compound has a prospect of being developed into an Mtb detection probe.

The invention relates to a tuberculosis antigen-specific host biomarker for diagnosing tuberculous pleural effusion and a kit, and belongs to the technical field of biological medicines. The host biomarker provided by the invention comprises the following components: IFN (interferon) gamma, IL-2 (interleukin-2), IL-12RB2 (interleukin-12R < 2 >), GZMB (Growth Zehnder Microorganism Blanket), IL-3 (interleukin-3), CXCL10 (C X-ray cell line 10), IL-12B (interleukin-12B), IL-18RAP ( According to the present invention, the mycobacterium tuberculosis RD1 region antigen or peptide fragment and the mycobacterium tuberculosis RD2 region antigen or peptide fragment are jointly used as the antigen irritants to stimulate the pleural effusion sample to be detected, and then the expression change of the host biomarker is detected so as to effectively identify and diagnose the tuberculous pleural effusion. By combining tuberculosis specific antigen stimulation and local infection microenvironment transcriptome analysis, the non-specificity defect of an existing host biomarker is overcome, and the host biomarker has high sensitivity and specificity in diagnosis of tuberculous pleural effusion.

The utility model discloses a petri dish for rapidly detecting mycobacterium tuberculosis, and particularly relates to the technical field of petri dishes, the petri dish comprises a supporting base, a supporting assembly is arranged on the supporting base, and the supporting assembly comprises a first storage cavity arranged in the supporting base; a second storage cavity is formed in the top of the first storage cavity, and a third storage cavity is formed in the top of the second storage cavity. According to the storage device, the supporting assembly is arranged, the friction force between the first storage cavity, the second storage cavity and the third storage cavity and the fingers of a worker is increased through the arrangement of anti-skid protrusions, and through the arrangement of staggered concave cavities, when the storage device is installed on the refrigeration equipment for storage, the refrigeration equipment can make close contact with the limiting base plate; and the low-temperature conduction preservation effect is ensured.

This invention relates generally to isolated monoclonal antibodies that binds to an epitope of the 10 kDa culture filtrate protein (CFP-10) or a peptide fragment thereof, and methods for using the same to detect Mycobacterium tuberculosis.

The invention belongs to the field of gene engineering, and particularly relates to a polyphosphate kinase mutant and application thereof. According to the invention, modification and screening are carried out on the basis of amino acids of wild type polyphosphate kinase PPK (polyP-polyphosphate kinase [EC: 2.7.4. 1]) from Mycobacterium tuberculosis, and the polyphosphate kinase with high enzyme activity, high catalytic efficiency and high ATP regeneration efficiency is obtained. The amino acid sequence of the polyphosphate kinase mutant is as shown in SEQ ID NO.11, and the polyphosphate kinase mutant is obtained by mutating valine at the 267th site of wild type polyphosphate kinase into glutamic acid and simultaneously mutating threonine at the 369th site into proline. Uridine monophosphate and cytidine monophosphate are produced through catalysis of the polyphosphokinase, the conversion yield is larger than 95%, meanwhile, cyclic regeneration of ATP is efficiently achieved, the ATP adding amount is reduced by 97%-98%, the product concentration is increased, the production cost is reduced, the catalysis application potential is greatly improved, and the important industrial application prospect is achieved.

The invention relates to the technical field of distributed computing, and discloses a distributed computing task scheduling optimization method and system for tubercle bacillus research data, and the method comprises the steps: extracting the CPU intensity, the memory demand, the I / O characteristic and the data dependence characteristic vector of a computing task; constructing a task dependence directed acyclic graph; determining a task execution sequence through topological sorting and key path analysis; constructing a multi-dimensional fitness matrix of task-resource matching; determining a task allocation scheme by adopting a scheduling strategy combining dynamic planning and heuristic search; predicting a resource demand through an adaptive resource reservation mechanism and dynamically adjusting the resource reservation amount; according to the method, collaborative optimization of accurate perception of task features and intelligent matching of resources is realized, and the resource utilization rate and the task processing efficiency are effectively improved.

A fast-acting disinfectant composition containing relatively high amounts of hydrogen peroxide, an organic sulfonic acid or salt thereof, and a carboxylic acid is disclosed. The fast onset disinfectant composition exhibits at least one of the following: efficacy on at least one non-enveloped or enveloped virus after a contact time of 15 seconds when tested according to ASTM test e1053; efficacy on at least one fungus after a contact time of 1 minute when tested according to the AOAC bactericidal spray / wipe method; efficacy on at least one bacterium after a contact time of 15 seconds when tested according to the AOAC bactericidal spray / wipe method; or efficacy on at least one Mycobacterium tuberculosis after a contact time of 1 minute during a Mycobacterium tuberculosis activity test according to an AOAC disinfectant.

The invention discloses a Mycobacterium tuberculosis real-time fluorescence isothermal amplification detection kit for internally controlling amplification efficiency through a color development method and application of the Mycobacterium tuberculosis real-time fluorescence isothermal amplification detection kit. The kit comprises an isothermal amplification primer aiming at mycobacterium tuberculosis, a probe modified by a fluorophore substance, an isothermal amplification primer aiming at an exogenous internal control gene, a pH value sensitive dye, an isothermal amplification enzyme and an endonuclease. According to the invention, isothermal amplification detection containing amplification efficiency internal control can be realized only by using a single fluorescent labeled probe, mutual interference among a plurality of fluorescent probes is reduced so as to ensure efficient and specific detection of target genes, and meanwhile, the cost of a detection reagent and the cost requirement of detection equipment are reduced.

The invention discloses a mycobacterium tuberculosis mutant detection crRNA and application of the mycobacterium tuberculosis mutant detection crRNA. One, two or more mismatched basic groups are arranged at the 5'end of crRNA, a section of basic group which can not be completely matched with a target section is arranged at the 3 'end of crRNA, and a loop-shaped bubble structure is formed, so that the effect of distinguishing specific mutant type and wild type mycobacterium tuberculosis is achieved, and meanwhile, the mutation point of a drug-resistant gene can be determined; the method has the advantages of low detection cost, short detection period, high sensitivity and good specificity.