33 results about "Retrotransposon" patented technology

Systems and methods for targeted gene modification, targeted insertion, perturbation of gene transcripts, and nucleic acid editing. Novel nucleic acid targeting systems comprise components of CRISPR systems and non-LTR retrotransposon elements.

The invention belongs to the technical field of plant biology, and particularly relates to a gene for regulating and controlling powdery mildew resistance of cucumbers and application of the gene, two cswrky31 mutants are obtained based on a cucumber Tnt1 reverse transcription transposon mutant library, and the two cswrky31 mutants both show high susceptibility to powdery mildew bacteria. A target gene Csa5G551250 which is directly regulated and controlled by CsWRKY31 is screened by virtue of a high-throughput transcriptome sequencing technology and a DNA affinity purification sequencing technology in combination with a yeast one-hybrid experiment. A dual luciferase report experiment shows that the CsWRKY31 positively regulates and controls the expression of the Csa5G551250, and the overexpression of the Csa5G551250 gene promotes the resistance of the cucumber to powdery mildew bacteria. A new reference gene resource is provided for cultivation of cucumber disease-resistant varieties, and a new thought is provided for molecular mechanism research of transcription factor mediated plant immunity.

Described herein are methods and compositions for accurate detection of cancer using ultrasensitive immunoassays, e.g., digital ELISA, to detect open reading frame 1 protein (ORF1p), which is encoded by the LINE-1 retrotransposon, in biofluids.

This technology provides targeted genome editing techniques for altering the color of parts of grape plants and their fruits. Specifically, this technology relates to using genome editing methods to generate edits in target genes that cause large fragment inactivation deletions, thereby altering the color traits of plants and fruits. These target genes include genes and genetic elements associated with plant color and pigment deposition (e.g., the PDS1 gene, the mybA1 gene, and the Gret1 retrotransposon).

The application discloses a sub-genome-specific retrotransposon of elephant grass and application thereof, and belongs to the field of bioinformatics and molecular cytogenetics. The application mainly comprises the following steps: 2 sub-genome-specific retrotransposon sequences are screened out through bioinformatics analysis of short fragment sequences of the sub-genome of elephant grass; primers are designed according to the sequences, and the genomic DNA of elephant grass is used as a template to amplify the primers through PCR, and the target fragments are recovered through purification of PCR products; and it is verified that the 2 retrotransposon sequences produce clear and bright signals on the respective 14 chromosomes of elephant grass through probe labeling, chromosome sheet preparation and fluorescence in situ hybridization. The retrotransposon sequences can be directly used for specific identification of the sub-genome of elephant grass, more accurate information is provided for identification of the sub-genome of elephant grass, and a foundation is laid for chromosome engineering breeding of elephant grass.

The present invention discloses an IRAP molecular marker primer for identifying Shazikongxin plum germplasm based on retrotransposons, characterized in that the IRAP molecular marker primer is one or a mixture of Ty1-6, Ty1-7, Ty1-9, Ty1-15, Ty1-18, Ty1-20, Ty1-25, Ty3-2, Ty3-3, Ty3-5, Ty3-6, Ty3-9, Ty3-14, and Ty3-16. The present invention detects the retrotransposons at the molecular level based on their characteristics, resulting in more accurate and reliable results. The detection was repeated on dozens of Shazikongxin plum germplasms and the test results remained consistent, indicating that the results were accurate and reliable.

Cells with stably integrated exogenous nucleic acid sequences (e.g., transgenes) into their genome are disclosed, said exogenous nucleic acid sequences being located within or near an integration site containing at least a portion of an endogenous retrovirus (ERV) or LTR-retrotransposon (LTR-RT), or replacing sequences containing ERVs or LTR-RTs that are part of or formerly part of the cell genome, as well as methods for producing and using such cells. Advantageously, high levels and / or stable production of transgene expression products can be achieved. Transgene integration and expression can be promoted by modulating cellular DNA repair pathways, for example, by transiently expressing genes encoding proteins that form part of DNA repair pathways during transgene integration.

Methods and compositions for modulating a target genome are disclosed.

The invention belongs to the technical field of plant biology, and particularly provides application of a CsTSR gene in cucumber corynespora leaf spot resistance. A transient expression strain and a cstsr mutant are respectively obtained through transient transformation of the CsTSR gene and a Tnt1 reverse transcription transposon mutant library, and the CsTSR gene is found to positively regulate the resistance of the cucumber to the corynespora leaf spot disease. The miR164d can regulate and control the CsTSR transcription factor in a targeted manner. A dual luciferase report and a GUS (glucuronidase) staining experiment result show that the miR164d negatively regulates and controls the CsTSR, and the CsTSR-mediated cucumber disease resistance can be enhanced by silencing the miR164d. The invention discloses a functional mechanism of the miR164d-CsTSR molecular module in cucumber pathogenic bacterium infection resistance, and provides a new reference gene resource for cultivation of cucumber disease-resistant varieties.

In X-linked dystonia-parkinsonism (XDP), an inherited SVA retrotransposon insertion in the TAF1 gene disrupts gene transcription, leading to a rare neurodegenerative disorder. The mechanism underlying aberrant TAF1 transcription remains elusive. We found that 5-ALA, a 5-ALA ester, a porphyrin or a pharmaceutically acceptable salt thereof for use is capable of mitigating the molecular phenotypes associated with the G4-quadruplex structures in the XDP- SVA in XDP-patient cells. The present invention demonstrates that treatment with 5-ALA, a 5-ALA ester, a porphyrin or a pharmaceutically acceptable salt thereof for use thereof significantly neutralized the main molecular phenotypes linked to XDP. The invention provides means and methods for the treatment of XDP or an individual carrying a genetic modification associated with XDP.

To provide methods and compositions for modulating a target genome.SOLUTION: The present disclosure relates to compositions, systems, and methods for targeting, editing, modifying, or manipulating DNA sequences (e.g., inserting a heterologous DNA sequence of interest at a target site in a mammalian genome) at one or more locations within the DNA sequence in a cell, tissue, or subject, e.g., in vivo or in vitro. The DNA sequence of interest may include, for example, a coding sequence, a regulatory sequence, or a gene expression unit. More specifically, the present disclosure provides a retrotransposon-based system for inserting a sequence of interest into a genome.SELECTED DRAWING: None

Methods and compositions for altering a genome at one or more locations in a host cell, tissue, or subject are disclosed.

The invention discloses a pennisetum purpureum subgenome specific retrotransposon and application thereof, and belongs to the field of bioinformatics and molecular cell genetics. The method mainly comprises the following steps: screening two subgenome specific reverse transcription transposon sequences through bioinformatics analysis of subgenome short fragment sequences of target grass; designing a primer according to the sequence, taking Pennisetum purpureum genome DNA as a template, carrying out PCR amplification, and purifying a PCR product to recover a target fragment; probe labeling, chromosome flaking and fluorescence in situ hybridization verify that the two reverse transcription transposon sequences generate clear and bright signals on respective 14 chromosomes of pennisetum purpureum. The reverse transcription transposon sequence disclosed by the invention can be directly used for specifically identifying the pennisetum purpureum subgenome, provides more accurate information for identification of the pennisetum purpureum subgenome, and lays a foundation for engineering breeding of the pennisetum purpureum chromosome.

This invention provides a method for preparing sweet and waxy maize with the same kernel size and its application, relating to the field of bio-breeding technology. This invention identifies and obtains for the first time a new sweet and waxy maize germplasm material, TZNB25341, formed by natural mutation. For the aforementioned new material TZNB25341, this invention conducts in-depth analysis of its key controlling genes for sweet and waxy maize with the same kernel size, and carries out systematic gene mapping and cloning studies on the genetic regulatory mechanism of this trait. It was discovered that in TZNB25341… ZmSH1 A 5979 bp retrotransposon LTR was inserted into the exon 13 region of the (Zm00001eb374090) gene. The insertion of this transposon led to three premature translation termination sequences and a significant decrease in transcription level in the exon, resulting in phenotypic changes in TZNB25341, which ultimately manifested as the sweet and sticky phenotype.

To provide a technique for discriminating Shine Muscat capable of reducing the labor of a worker and rapidly discriminating the food.SOLUTION: The method for discriminating Shine Muscat includes a step of amplifying retrotransposon VINE1 or Tvv1 and a region on a genome adjacent to retrotransposon using DNA of grape to be discriminated as a template by using a primer set including a primer having a base sequence specifically binding to retrotransposon VINE1 or Tvv1 contained in the genome of Shine Muscat and a primer specifically binding to a genomic region located in the vicinity of retrotransposon VINE1 or Tvv1 contained in the genome.SELECTED DRAWING: None

This application provides a retroposophyte system for editing the genome and its use therein, wherein the retroposophyte in the retroposophyte system comprises an amino acid sequence as shown in any one of SEQ ID NO:1-158, SEQ ID NO:475-489, SEQ ID NO:515-520 and SEQ ID NO:522-530, or comprises an amino acid sequence having at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identity with any one of SEQ ID NO:1-158, SEQ ID NO:475-489, SEQ ID NO:515-520 and SEQ ID NO:522-530; or the retroposophyte is a truncated version of an amino acid sequence as shown in any one of SEQ ID NO:1-158, SEQ ID NO:475-489, SEQ ID NO:515-520 and SEQ ID NO:522-530. This application also relates to engineered sequences of retrosockase coupled with other amino acid sequences; and engineered sequences of truncated retrosockase coupled with other amino acid sequences.

Non-LTR retrotransposon systems are provided. Isolated functional proteins encoded by retrotransposons, nucleic acids encoding functional proteins, nucleic acid sets, nucleic acid constructs, compositions, recombinant vectors, recombinant host cells, and kits are provided. Methods for introducing exogenous nucleic acid fragments into the genome of a host cell, methods for editing the genome of a host cell, and methods for obtaining a host cell containing exogenous nucleic acid fragments in its genome are also provided. Further, the use of functional proteins encoded by retrotransposons, nucleic acids, nucleic acid sets, nucleic acid constructs, compositions, recombinant vectors, or recombinant host cells for introducing exogenous nucleic acid fragment genes into the genome of a host cell, or for preparing drugs or formulations, is provided.

Methods and compositions comprising a template RNA comprising a modified 5' LTR and modified 3' LTR for altering a genome at one or more locations in a host cell, tissue, or subject are disclosed.

The invention discloses a dht mutant gene for regulating and controlling the plant type, the panicle shape and the grain shape of rice, a mutant and application, the sequence of the mutant gene is as shown in SEQ ID No.1, and compared with an original gene Os07g0129700, a reverse transcription transposon fragment with the length of 1375 nucleotides as shown in SEQ ID No.2 is inserted into the fourth intron of the original gene Os07g0129700. The dht mutant gene provided by the invention can realize the synergistic improvement of the plant type, panicle shape and grain shape of rice, meanwhile, the yield and quality of the rice are improved, and the dht mutant gene has important breeding utilization value; a specific molecular marker for detecting the dht mutant gene is combined with rice plant type observation, so that the dht mutant gene in the mutant can be quickly introduced into a target rice variety, and the synergistic improvement of the yield and the quality of the dht mutant gene is realized.

Methods and compositions for altering a genome at one or more locations in a host cell, tissue, or subject are disclosed.

Engineering the oleaginous yeast Rhodosporidium toruloides to produce pomegranate-derived punicic acid and pomegranate-derived punicic acid accumulation in Saccharomyces cerevisiae by Ty retrotransposon-targeted random gene shuffling.

Methods and compositions for altering a genome at one or more locations in a host cell, tissue, or subject are disclosed.

The invention discloses a cotton fiber yield-related NAC protein coding gene GhIFY molecular marker and application thereof, and belongs to the field of biotechnology application. The promoter sequence of the cotton NAC protein coding gene GhIFY has two haplotypes, the promoter sequence of the haplotype 1 is as shown in SEQ ID NO.1, the promoter sequence of the haplotype 2 is as shown in SEQ ID NO.2, and compared with the haplotype 2, a 5103bp retrotransposon is inserted at the-1634bp position of the promoter sequence of the haplotype 1; the fiber yield and lint percentage character of the haplotype 1 is superior to that of the haplotype 2. A to-be-detected cotton germplasm material carrying the NAC protein coding gene GhIFY with a retrotransposon sequence shown as SEQ ID NO.1 is selected as a high-yield character cotton germplasm for cotton breeding. The molecular marker has important effects and application prospects in improvement of cotton fiber yield traits and cultivation of new high-yield cotton varieties.

The invention belongs to the technical field of plant biology, and particularly relates to a gene for regulating and controlling powdery mildew resistance of cucumbers and application of the gene, two cswrky31 mutants are obtained based on a cucumber Tnt1 reverse transcription transposon mutant library, and the two cswrky31 mutants both show high susceptibility to powdery mildew bacteria. A target gene Csa5G551250 which is directly regulated and controlled by CsWRKY31 is screened by virtue of a high-throughput transcriptome sequencing technology and a DNA affinity purification sequencing technology in combination with a yeast one-hybrid experiment. A dual luciferase report experiment shows that the CsWRKY31 positively regulates and controls the expression of the Csa5G551250, and the overexpression of the Csa5G551250 gene promotes the resistance of the cucumber to powdery mildew bacteria. A new reference gene resource is provided for cultivation of cucumber disease-resistant varieties, and a new thought is provided for molecular mechanism research of transcription factor mediated plant immunity.

The invention discloses a molecular marker closely linked with a wild apple peel color, a detection primer and application of the molecular marker and the detection primer, and belongs to the technical field of genetic breeding. The molecular marker is located at a site 31503385-31511919 of a No.9 chromosome of a catalpa bungei genome, and is an insertion / deletion polymorphic site of an LTR retrotransposon in an upstream promoter region of an MdMYB1 gene. The invention further designs a detection primer of the molecular marker, and the nucleotide sequence of the detection primer is SEQ ID NO.2 and SEQ ID NO.3. When the amplification product of the detection primer has a strip at 536bp, the amplification product is an insertion genotype (red peel), and when the amplification product does not have a strip, the amplification product is a deletion genotype (yellow or yellow green peel). The method can quickly and accurately judge the pericarp color of the wild apple species filial generation, realizes early screening of coloring characters, greatly improves the cross breeding efficiency of wild apples, provides key technical support for genetic improvement and directional utilization of wild apple resources, and has a wide application prospect.

The application discloses a Idahoa genus germplasm identification IRAP marker, and an IRAP primer used in the IRAP marker is designed based on a RT sequence of an Idahoa genus retrotransposon, wherein the specific IRAP primer used in the IRAP marker is selected from one or more sequences shown in the following: primer IRAP07, sequence 5'-GCCGACCAT TGTTGTTATGT-3'; primer IRAP19, sequence 5'-AGGCGTCTGTTTGAGACCAT-3'; primer IRAP52, sequence 5'-GCTGTATGCCAAATTGAGCA-3'; primer IRAP56, sequence 5'-CCAAGTAAGGCTGGAAAACC-3'; primer IRAP59, sequence 5'-CCGCGGATAGATGACTTGTT-3'; primer IRAP65, sequence 5'-TAGTGATGCCATTCGGGTTA-3'; and primer IRAP73, sequence 5'-ACTTCCGCGGATAGATGACT-3'. The marker is convenient and fast to operate, stable in result, high in efficiency, not easy to be affected by environment, and can be applied to researches such as Idahoa genus germplasm identification, genetic diversity evaluation and species genetic relationship analysis, thereby providing an effective means for excellent germplasm mining, assisted breeding and germplasm property right protection.

The present disclosure provides systems and methods for transferring a cargo nucleotide sequence to a target nucleic acid site, which may include a first double-stranded nucleic acid comprising a cargo nucleotide sequence configured to interact with a retrotransposase, and the retrotransposase configured to transfer the cargo nucleotide sequence to the target nucleic acid site.

The invention provides an FSH (follicle stimulating hormone) beta gene-based molecular marker for the reproductive performance of a Branchiotai Tibetan pig, a primer of the molecular marker and a method for screening a high-reproductive-performance Branchiotai Tibetan pig individual by using the marker, and the sequence of the molecular marker is a nucleotide sequence as shown in SEQ ID No: 1. The polymorphism of the insertion mutation site of the 292bp retrotransposon in the intron 1 of the FSH beta gene of the chang-dug Tibetan pig is shown as three genotypes, namely AA, AB and BB, wherein the AA type is a dominant genotype; a forward primer of a primer sequence of the molecular marker has a nucleotide sequence as shown in SEQ ID No: 2, and a reverse primer of the primer sequence of the molecular marker has a nucleotide sequence as shown in SEQ ID No: 3. The primer provided by the invention can specifically amplify the DNA sequence around the insertion mutation site of the 292bp retrotransposon in the intron 1 of the Tibetan pig FSH beta gene, the detection efficiency is high, and the reproductive performance of the Tibetan pig can be remarkably improved and the large-scale development of the Tibetan pig industry can be promoted when the primer pair is used for molecular marker-assisted breeding.

The present disclosure demonstrates that earlier editing of cells with ribosomal non-LTR retrotransposons (R elements) still allowed sufficient editing from subsequent dosing of R elements, without significant interference therebetween, whether they are the same R elements or different. Provided are compositions and methods for integrating one or more exogenous sequence(s) to the genome of a mammalian cell, comprising (a) introducing to the cell a first amount of one or more polynucleotide(s) comprising a first ribosomal non-long terminal repeat (LTR) retrotransposon (R element) which comprises a first exogenous sequence, and, (b) introducing to the cell a second amount of one or more polynucleotide(s) comprising a second R element.