33 results about "Corynespora" patented technology

The invention discloses broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of the broad-spectrum disease resisting, growth promotion and stress resisting bacillus. The broad-spectrum disease resisting, growth promotion and stress resisting bacillus is bacillus sp. WXCDD105, and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation address is 3, No.1 Yard, Western Beichen Road, Chaoyang District, Beijing, the preservation date is May 25, 2016, and the preservation number is CGMCC No.12496. A biocontrol strain provided by the invention has ultraviolet-resisting and drought-resisting properties, has stronger prevention and treatment effects on the tomato gray mold and the tomato leaf mold, and can inhibit fungal diseases including wilt fusarium of a plurality of types of crops, corn foot root bacteria, rice wilt pathogens, acanthopanax senticosus root rot pathogens, corynespora cassiicolai, colletrichum orbiculare, exserohilum turcicum, tomato spot blight fungi, sunflower sclerotinia sclerotiorum, botrytis cinerea, fusarium oxysporum, verticillium fusarium, alternaria alternata and the like; the broad-spectrum disease resisting, growth promotion and stress resisting bacillus has a growth promotion effect on tomato seeds and seedlings and can also improve the quality of fruits and reduce the rotting rate.

The invention discloses an Isaria javanicanicus mould strain and application thereof. The high-efficiency Isaria javanicanicus (Friedrichs & Bally) strain Ij01 is classified to belong to Corynespora, Hyphomycetaceae, Hyphomycetales, Hyphomycetes, Mitosporic Fungi, Eumycota. The strain has been preserved in China Center for Type Culture Collection (CCTCC), the preservation date is Oct 27, 2008, and the preservation number is CCTCC M 208171. The high-efficiency Isaria javanicanicus mould is entomopathogenic fungi to have high efficiency for preventing and controlling prodenia litura, has the characteristics of brand-new action mechanism different from the prior chemical insecticides, and no environmental pollution and residue when taken as a living biological pesticide, and meets the requirement of organic food production.

The invention discloses 1,2,4-triazole Mannich base derivatives containing substituted piperazidine, and a preparation method and application thereof. The structural formulae of the 1,2,4-triazole Mannich base derivatives containing substituted piperazidine are disclosed as general formulae I and II, wherein X, Y and Z are defined as Claim 1. The compounds have high bactericidal activity, have high in-vitro inhibition activity for ring rot of apple, sheath blight of wheat, scab of wheat, early blight of tomato and other plant pathogens, have favorable in-vivo control effects on corynespora leaf spot of cucumber, bacterial spot of cucumber, gummy stem blight of cucumber, downy mildew of cucumber, sclerotinia rot of cucumber and other plant fungi, and especially have special effects for bacterial spot of cucumber and Pseudoperonospora cubensis Rostov. The compounds disclosed as general formula I and general formula II have weeding activity. The 1,2,4-triazole Mannich base derivatives are suitable for integrated control of fungus attack and crop smothering on various crops.

The invention provides streptomyces hygroscopicus, which is named streptomyces hygroscopicus PA-4 and is preserved in Guangdong Microbial Culture Collection Center on May 24, 2021, and the preservation number is GDMCC No.61679. The strain has stable and broad-spectrum bacteriostatic activity and has a relatively strong bacteriostatic effect on various common crop pathogenic fungi such as fusarium oxysporum, colletotrichum, cryptoscoparia, corynespora, alternaria, fusarium and the like, a fermentation crude extract of the strain can influence normal growth and development of Foc4 mycelia, spores and the like and has a poisoning or inhibiting effect on Foc4 germs. In addition, the streptomyces hygroscopicus disclosed by the invention can also inhibit pathogenic bacteria from infecting plant root systems and promote plant growth, expands a new field for preventing and treating various plant diseases such as fusarium wilt, promoting plant growth and the like, and has a good development and application prospect.

The invention discloses a fungicide composition of fluoride containing ether bacteria amide and validamycin, which consists of active components and supplementary materials. The active components are fluoride ether bacteria amide and validamycin, the weight ratio of fluoride ether bacteria amide to validamycin is 1:4-4:1, and the total content of the active components is 10%-60%. The fungicide composition provided by the invention is specially utilized to prevent and control rice sheath blight disease, pyricularia grisea, rice false smut, wheat sharp eyespot, vegetable leaf spot disease, vegetable powdery mildew, pear scab, coniothyrium diplodiella, apple anthracnose, macrophoma kawatsukai, cucumber corynespora target leaf spot and strawberry fusarium wilt and the like.

The invention relates to a resistance identification method of the cucumber corynespora leaf spot disease. The method includes the steps that a fresh, healthy and complete cucumber true leaf serves as an infection identification material; an improved Czapek liquid culture medium is used for culturing corynespora cassiicola, and primary separation and extraction are performed on culture filtrate to obtain crude toxin filtrate of the corynespora cassiicola; the back of the cucumber detached leaf is inoculated with the crude toxin filtrate in a non-destructive hanging drop mode; then, moisturizing culture is performed in an illumination culture box; the leaf area infected by the toxin filtrate is inspected through a crossing method after the third day, and then the resistance of the cucumber variety to the corynespora leaf spot disease caused by the corynespora cassiicola is obtained. Through the method, the resistance of different varieties of cucumbers to the corynespora cassiicola can be rapidly and accurately identified, and the identification period is greatly shortened. The method is easy to implement, high in repeatability and applicable to resistance identification of the cucumber corynespora leaf spot disease.

The invention discloses a preparation method of a citrus pulp-based fermentation preparation, which comprises the following steps: uniformly mixing citrus pulp with auxiliary materials to obtain a solid culture medium; providing zymophyte, and activating and culturing the zymophyte; and inoculating the activated and cultured zymophyte into the solid culture medium for fermentation culture to obtain a citrus pulp-based fermentation preparation finished product. The auxiliary materials are selected from rice bran and/or wheat bran, and the zymophyte is composed of bacillus licheniformis, corynespora aspera and aspergillus niger. Correspondingly, the invention further discloses the citrus pulp-based fermentation preparation prepared by adopting the preparation method and application of the citrus pulp-based fermentation preparation. By implementing the method, the contents of flavones, organic acids and amino acids in the citrus pulp-based fermentation preparation can be effectively increased, and the nutritional value of the citrus pulp-based fermentation preparation is increased.

The invention provides application of a compound H2 in preparation of a lipopeptide bacteriostatic preparation. The structural formula of the compound H2 is shown as a following formula (please see the formula in the description). The compound H2 is obtained by studying and separating the specific active ingredients of fermentation liquid of bacillus amyloliquefaciens HAB-2, has the wide activity on inhibiting tropical pathogenic fungi, has the effect of promoting growth of some plants, has the preservation effect on fresh fruits, can be applied to preparation of the lipopeptide bacteriostatic preparation and prevention and control of plant fungus diseases, particularly can be applied to tomato early blight, watermelon fusarium wilt, banana fusarium wilt, cotton fusarium wilt, rubber tree anthracnose, a rubber tree corynespora leaf fall disease, tea grey blight, mango anthracnose and/or a rubber tree brown root rot disease and the like and is worthy of application and popularization.

The invention relates to a method for preparing a medicament for preventing and controlling a corynespora leaf-fall disease of rubber trees. The method comprises the following steps: (1) weighing corymbose hedyotis herb, adding ethanol water with the volume percent concentration of 70-90 percent and the weight of 3-6 times of that of the corymbose hedyotis herb, and heating for reflux extraction for 1-3 hours; filtering, reducing the pressure of filtrate and recovering ethanol, concentrating until no ethanol is recovered, and obtaining an extracting solution 1; (2) weighing allophylus viridis, adding water with the weight of 3-6 times of that of the allophylus viridis, heating for 3-6 hours under the reflux condition, and cooling to a room temperature; filtering, adding ethanol in filtrate until the content of ethanol is 50-80 volume percent, and placing for 12-48 hours; filtering, reducing the pressure of the filtrate and recovering ethanol, concentrating until no ethanol is recovered, and obtaining an extracting solution 2; (3) merging the extracting solutions. The medicament for preventing and controlling the corynespora leaf-fall disease of the rubber trees is a good medicament for preventing and controlling the corynespora leaf-fall disease of the rubber trees in the fields, and the effect is superior to that of some usual antibacterial agents.

The invention relates to a medicament for preventing and controlling the corynespora leaf fall disease of a rubber tree. The medicament comprises the following raw materials in parts by weight: 100-300 parts of corymbose hedyotis herb and 100-300 parts of allophylus viridis. The medicament for preventing and controlling the corynespora leaf fall disease of the rubber tree is relatively good and is superior to certain common microbicides in effect.

The invention discloses molecular markers for detecting cucumber corynespora leaf spot bacteria and application thereof. The molecular markers with sequences comprise a molecular marker P1 with SEQ ID NO.1 to 2, a molecular marker P2 with SEQ ID NO.3 to 4, and a molecular marker P3 with SEQ ID NO.5 to 6. The molecular markers have the advantages that (1) the cucumber corynespora leaf spot bacteria can be accurately, quickly, sensitively and practically detected; (2) the sensitivity on the detection of pathogenic bacteria is high, and the molecular markers can be applied to the quick detection of the cucumber corynespora leaf spot bacteria in disease plants.

The invention discloses a preparation method of a botanical extract biopesticide preparation and an application thereof, and relates to a preparation method and an application of a biopesticide preparation. A cowherb seed extract containing multiple components is processed into a preparation to be used for preventing and treating common plant diseases, and the preparation is processed into soluble liquid, microemulsion, emulsion in water, a suspending agent, water dispersible granules and the like, and is used for preventing and treating fungal diseases such as cucumber corynespora leaf spot, tomato early blight, cucumber downy mildew, wheat scab and the like, and bacterial diseases such as cucumber bacterial angular leaf spot, citrus fester disease, Chinese cabbage soft rot and the like. The biological pesticide can be applied to plant virus diseases such as tobacco mosaic virus (TMV), potato Y virus (PVY), tomato yellow leaf curl virus (TYLCV), tomato spotted wilt virus (TSWV), rice stripe disease, pepper virus disease and the like, accords with national carbon peak reaching and carbon neutralization policies, maintains national grain safety, environmental safety and health and safety guarantee of people, and has wide application prospects and conversion values.

The invention provides application of piperlongumine in preventing and treating the plant pathogenic fungi. The test concentration of the piperlongumine serving as a bactericide for preventing and treating the plant pathogenic fungi is larger than or equal to 200 mg/L; as an antibacterial active component, the piperlongumine can be used for preventing and treating fusarium wilt, botrytis cinerea, gibberellic disease, physalospora piricola, alternaria alternata, peronophythora, northern leaf blight and corynespora fallen leaf, the inhibition rate is 80% or above, and meanwhile, the piperlongumine also has relatively strong protection and treatment effects on botrytis cinerea. Tests show that the piperlongumine has a broad-spectrum bactericidal effect on plant pathogenic fungi, and a foundation is laid for further utilizing piperaceae plant piper longum to create a novel botanical fungicide.

The invention relates to a method for bioconverting fumaric acid to produce L-malic acid. Using the self-selected Corynespora lusitaniae CICC 33054 (Clavispora lusitaniae CICC 33054), the yeast cells were obtained by fermentation and centrifugation. Add sodium fumarate solution to yeast cells for biotransformation, replace precipitation with calcium fumarate, filter, sulfuric acid hydrolysis, filter, concentrate, crystallize and dry to obtain L-malic acid product. Corynespora portuguese CICC 33054 is easy to cultivate and has high transformation efficiency, and its use in the production of L-malic acid can improve production efficiency and reduce production costs.

The invention discloses a preparation method of bio-based end-capped polyether, which comprises the following steps: mixing and fermenting geotrichum candidum, corynespora aspera and pichia membranaefaciens in a fermentation culture medium taking hydroxyl-containing polyether as a substrate, and finally preparing the end-capped polyether through the action with an end-capping reagent, the preservation number of the geotrichum candidum is CCTCC No.M2013114; the preservation number of the corynespora aspera is CCTCC No.M205116; and the preservation number of the pichia membranaefaciens is CCTCC No.M205117. The method provided by the invention is simple and economical, can generate terminated polyether in one step, and is beneficial to continuous production.

The invention discloses a cucumber CsSTK gene, a protein, an expression vector and application, and belongs to the technical field of molecular biology. The invention discloses a cucumber CsSTK gene. The nucleotide sequence of the cucumber CsSTK gene is as shown in SEQ ID NO: 1; the amino acid sequence of the protein coded by the CsSTK gene is shown as SEQ ID NO: 2; the invention also discloses an expression vector containing the CsSTK gene. The invention also discloses an application of the CsSTK gene or the protein or the expression vector in improving the resistance of the cucumber corynespora leaf spot disease. Genetic engineering verifies that a transgenic plant obtained through overexpression of CsSTK is inoculated and identified, the disease symptom of the cucumber corynespora leaf spot is obviously relieved, and it is indicated that the overexpression of CsSTK can obviously enhance the resistance to the cucumber corynespora leaf spot disease.