The invention discloses an
InDel molecular marker linked with cucumber
corynespora leaf spot, a PCR (
Polymerase Chain Reaction) detection primer, a kit and application. According to the invention, a resistant cucumber material and a high-susceptibility material are utilized to construct an F2 group, and BSA-Seq, a
molecular marker and other methods are utilized to preliminarily locate a cucumber
corynespora leaf spot resistant
gene in an interval of about 5.0 Mb in 19 Mb-24 Mb on Chr5; the
InDel molecular marker closely linked with the resistance to the
corynespora leaf spot disease is developed, and the
InDel molecular marker is applied to molecular marker detection and
seedling-stage
disease resistance identification on 143 cucumber test varieties, so that the high correlation between the molecular marker and the resistance to the cucumber corynespora leaf spot
disease is verified; whether cucumbers contain the anti-corynespora leaf spot
gene or not can be simply and rapidly detected, then the cucumber anti-corynespora leaf spot disease resistance is detected or detected in an auxiliary mode, and the molecular marker has an application prospect in cucumber anti-corynespora leaf spot molecular marker auxiliary breeding.