Streptomyces having inhibiting effect on Corynespora cassiicola and application of Streptomyces

A technology of Corynebacterium multiprime and Streptomyces, which is applied in the application, chemicals for biological control, bacteria, etc., can solve the problems of lack of attention, economic loss, infection of Corynebacterium multiprime, etc., and is not easy to produce. The effect of drug resistance and no environmental pollution

Active Publication Date: 2017-02-15
HUBEI BIOPESTICIDE ENG RES CENT
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In 1958, it was first reported in India that Corynebacterium multiprimum caused Corynemycetes defoliation (Ramakrishnan and Pilla, 1961), and the disease occurred successively in the main rubber-producing areas of Asia-Pacific and Africa in the 1950s and 1980s. However, because the occurrence of the disease is limited to nurseries and young plants and the damage is not serious, it has not attracted people's attention as a secondary disease.
However, in recent years, disease investigations have found that plant diseases caused by Corynemycetes multiprime have seriously affected the production of many types of economic crops in the world, and caused huge economic losses. , a bunch of red, poinsettia, water boxwood, cucumber, tomato, pepper and other horticultural crops Corynesporium leaf spot; The loss reaches 11,800 kg / ha (Pernez ny et al., 1996), and it is estimated that once severe Corynesporium leaf defoliation occurs, the yield loss of rubber trees will be nearly 20%; in addition, in Brazil, the Philippines, Nigeria, India and South Korea Corynesporium leaf spot has also been reported in Asian countries such as Japan and Japan (Chee, 1990)
[0004] In my country, Wei Jingchao first discovered cowpea leaf spot in the lower reaches of the Yellow River in 1950, but due to the limited planting area of ​​cowpea, it has not attracted much attention.
In the early 1990s, cucumber and eggplant vegetable cultivation fields in Henan, Liaoning and other places in my country were generally infested by Corynebacterium polybasicus, causing certain economic losses.
But the actinomycetes isolated at present are only 0.1-1% of the actual species, so it has great application and development space, and there is no anti-corynebacterium leaf spot of live actinomycetes and the antibiotics produced at present. to report

Method used

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  • Streptomyces having inhibiting effect on Corynespora cassiicola and application of Streptomyces
  • Streptomyces having inhibiting effect on Corynespora cassiicola and application of Streptomyces
  • Streptomyces having inhibiting effect on Corynespora cassiicola and application of Streptomyces

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Experimental program
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Effect test

Embodiment 1

[0035] A Streptomyces strain with good inhibitory effect on Corynebacterium multiprime was isolated from the soil collected from Moshan, Donghu Lake, Wuhan. It was identified by 16s rDNA as Streptomyces phaeoluteichromatogenes. It was identified on December 23, 2015. It was sent to the China Collection of Type Cultures for preservation, and was classified and named: Streptomy ces phaeoluteichromatogenes HEBRC-45958; the preservation number is CCTCC NO: M2015768; Location: Wuhan University, Wuhan, China. In the present invention, Streptomyces phaeoluteichromatoge nes HEBRC-45958 or referred to as HEBRC-45958.

[0036] Morphological characteristics: It grows well on ISP-2 medium, the colony is brownish-yellow, and the spore surface is smooth ( figure 1 ).

[0037] Physiological and biochemical characteristics of the strain:

[0038] Table 1 Carbon source utilization of strain HEBRC-45958

[0039]

[0040]

[0041] +: positive, -: negative

Embodiment 2

[0043] The preparation method of Streptomyces tannifera HEBRC-45958, comprising:

[0044] The slant-preserved strains were activated on ISP-2 plates, picked and inoculated into 100 ml of seed medium, and cultured for 4 days at 28°C on a shaker at 160 rpm; the seed liquid was obtained.

[0045] The formulation of the seed medium is the same as that of the fermentation medium.

[0046] The seed liquid of Streptomyces sp. HEBRC-45958 was transferred into the fermentation medium at an inoculum of 5% (V / V). The temperature is 28° C., the stirring speed of the fermentation tank is 300 rpm, and the filling amount of the fermentation medium is 80% of the volume of the 20L tank; the fermentation is sufficient for 6 days; the prepared fermentation broth is used in Example 4.

[0047] The formula of the fermentation medium includes: 2% of mannitol, 1.5% of soybean peptone, 0.3% of yeast powder, 0.5% of calcium carbonate, 0.5% of sodium chloride; pH value of 7, all the above are mass rat...

Embodiment 3

[0049] In vitro inhibitory effect of S.phaeoluteichromatogenes HEBRC-45958 on C.cassii cola:

[0050] The slant-preserved strain HEBRC-45958 was activated on ISP-2 plate, cultured at 28°C for 4 days, and a 4mm bacterial plate was punched out with a hole punch and placed in the center of the PDA plate. The slant-preserved C. cassiicola was activated on PDA medium for 5 days, and four 4 mm plates were taken and placed 25 mm away from the HEBRC-45958 plates. The antibacterial zone was observed after culturing for 7 days. The hyphae of C. cassiicola and HEBRC-45958 confronting the border were picked, and the morphological difference of the hyphae under the microscope was treated and compared with the control.

[0051] The confrontation between HEBRC-45958 and C. cassiicola produced an obvious inhibition zone with a width of 25 mm ( figure 2 ), with a strong inhibitory effect, the treated hyphae were twisted, swollen and deformed compared to the control hyphae ( image 3 ).

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Abstract

The invention discloses Streptomyces having an inhibiting effect on Corynespora cassiicola and application of the Streptomyces. The Streptomyces is Streptomyces phaeoluteichromatogenes HEBRC-45958, and the preservation number of the Streptomyces phaeoluteichromatogenes is CCTCC No. M2015768. The Streptomyces has the advantages that the Streptomyces phaeoluteichromatogenes can generate fermentation broth with high antibacterial activity after fermentation, and the fermentation broth is good in preventing and treating effect on Corynespora leaf spot caused by the Corynespora cassiicola; compared with current chemically-synthesized bactericide, the Streptomyces phaeoluteichromatogenes comes from the natural environment and is efficient, low in toxicity, free of environment pollution, less prone to tolerance generating, and the like.

Description

technical field [0001] The invention belongs to the technical field of crop prevention and control, and in particular relates to a Streptomyces strain with an inhibitory effect on Corynebacterium multiprime and its application. Background technique [0002] Corynespora cassiicola, belonging to the subphylum Deuteromycota, Hychospora, Hychospora, Duromycetaceae, Corynespora, is a worldwide distribution of plant pathogenic fungi with a wide range of hosts. The earliest species found in this genus with the widest range of hosts. It can infect 530 species of plants in nearly 380 genera in tropical and subtropical regions, including cucumber, tomato, cotton, cowpea, soybean, papaya, tobacco and rubber (Smith, 2008). The fungus mainly damages the leaves of plants, forming typical necrotic spots with yellow halos, and can also infect roots, stems, flowers and fruits, causing defoliation and fruit drop in severe cases. [0003] Corynebacterium polyphylla was first reported in Indi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/20C12N1/205C12R2001/465
Inventor 黄大野万中义曹春霞刘晓艳程贤亮姚经武杨自文
Owner HUBEI BIOPESTICIDE ENG RES CENT
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