277results about How to "Increase production levels" patented technology

The invention discloses a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat sequences)-Cas (CRISPR-associated proteins) system in Streptomyces virginiae IBL14 and a gene editing operation method using the CRISPR-Cas system. The method comprises the processes of carrying out design and plasmid construction on features, targeted genes and gene editing templates of a CRISPR-Cas I-B type gene editing system and carrying out gene editing operation in the Streptomyces by a targeted gene and gene editing template recombinant plasmid. Through carrying out knockout, insertion, traceless point mutation and RNA (Ribonucleic Acid) level regulation and control on genetic genes by using the CRISPR-Cas I-B type gene editing system in the Streptomyces virginiae IBL14, the hereditary features of organisms can be conveniently changed; according to the CRISPR-Cas system and the method for carrying out gene editing by using the CRISPR-Cas system, novel technologies and methods are provided for improving the production level of products of medicine, food and other industries and improving the quality of the products.

The invention discloses a method for producing glutathione by using bifunctional glutathione synthetase contained in actinobacillus pleuropneumonia, actinobacillus succinogenes, bacillus cereus, streptococcus sanguis, streptococcus gordonii, streptococcus uberis and streptococcus thermophilus. The method comprises heterologous expression by using the seven microbes or enzymes of the microbes in other microbes. The glutathione synthesized by using the method has the structure of natural glutathione, and has high response rate and high output and yield of the glutathione.

The invention relates to the technical field of agricultural production, and particularly provides a monitoring method for crop diseases and pests. According to the technical scheme of the invention, the method is conducted based on the unmanned plane technology and the cloud computing technology. The method comprises the steps of establishing a crop disease and pest database, shooting the image of a field crop through the cruising of an unmanned plane, calculating, pre-warning, accurately shooting, calculating for a second time, guiding the disease and pest control and prevention, and the like. On the basis of establishing the crop disease and pest database, the image data of the field crop are shot by the unmanned plane. Through the cloud computing-based analysis and comparison, the early warning for crop diseases and pests is given out in time. Meanwhile, the disease and pest control and prevention is guided and the agricultural production level is improved. The agricultural production management cost is reduced. Moreover, the yield, the quality and the benefit of agricultural products are improved.

Belonging to the technical field of corn starch production, the invention in particular relates to a production improvement method for corn starch. The method consists of a purification process, an acid making process, a soaking process, a crushing section, germ separation drying, fiber washing and drying, protein concentration drying, starch milk refining, as well as starch dehydration and drying. The method is characterized in that the technical scheme of adding a compound enzyme composed of cellulose, xylanase, pectinase and mannanase into a crushed corn slurry plays a significant role in separating all corn components, thus facilitating production and processing of corn starch, and greatly improving the production level of corn starch.

The invention discloses a method for producing glutathione by fermentation of recombinant Escherichia coli. The method comprises the following stages of: (1) carrying out batch culture or fed-batch culture to obtain a great deal of recombinant Escherichia coli cells expressing exogenous bifunctional glutathione synthetase, wherein the bifunctional glutathione synthetase is protein which simultaneously has activities of gamma-glutamyl cysteine synthetase and glutathione synthetase; (2) carrying out over-expression on the bifunctional glutathione synthetase obtained in the stage (1); and (3) adding L-glutamic acid, L-cysteine and glycine precursor amino acids to realize high-efficiency production of glutathione by fermentation. According to the invention, the recombinant Escherichia coli strain expressing the exogenous bifunctional glutathione synthetase is used for producing glutathione by fermentation, so that the method not only has high GSH (Glutathione) synthesizing capability, but also is free from GSH feedback inhibition, and has the advantages of rapid reaction, high glutathione yield and short production period.

The present invention discloses a construction method of escherichia coli for synthesizing fucosylated oligosaccharide by fermentation. The construction method includes the steps: (1) overexpressing at least one gene for encoding enzymes required for denovo synthesizing GDP-L-fucose in a prokaryotic host cell; (2) expressing an exogenous gene for encoding a fucosyltransferase in the prokaryotic host cell; (3) reducing or eliminating the activity of GDP-mannanohydrolase in the prokaryotic host cell; and (4) reducing or eliminating the activity of beta-galactosidase in the prokaryotic host cell.According to the present invention, the escherichia coli is constructed by using the method, and the escherichia coli may be used for preparing the fucosylated oligosaccharide. Shown by a result generated by performing fermentation production verification on the fucosylated oligosaccharide (with 2'-fucosyllactose as an example) in a 5L tank by adopting an engineering strain constructed by the present invention, the highest production level of the 2'-fucosyllactose (2'-FL) may reach 50 g / L.

The invention belongs to the field of middle titanium slag melting technology, specifically to a middle titanium slag blast furnace smelting method. The technical problems the invention object to resolve is to provide a middle titanium slag melting method in a medium-sized blast furnace, the method can have a better use of schreyerite resources to perform middle titanium slag melting, specifically adopting 25 - 30 parts of all-vanadium-titanium pellet ore, 65 - 70 parts of low-vanadium-titanium magnetite ore and 5 - 10 parts of lump ore as raw materials for smelting in blast furnace. Obtained cast iron satisfies the quality requirements for further steelmaking, the furnace cinder dyadic alkalinity is 1.15 - 1.30, and can be used raw material of the composite powder. The method of the invention uses an appropriate charging stock structure in the blast furnace to reduce the sintering powder and to improve the air permeability, and makes full use of high cost-effective of schreyerite to effectively reduce production costs.

The invention discloses an agricultural product production and marketing integration service platform based on Internet and large data. The platform comprises a data excavation subsystem used by a user and an agricultural data analysis subsystem, wherein the data excavation subsystem is used for effective collection needed by the user and excavating data; and the agricultural data analysis subsystem is used for carrying out refinement management on agricultural production In the invention, production and market demands are tightly cooperated, an effective service is realized and an original profit mode is changed; the service is integrated with a production and marketing service of agricultural products, services are provided for a consumer, an agricultural producer or a cooperative, and a final purpose is to realize a customized and multi-type C2B order type agricultural production and marketing service mode; and the agricultural product production and marketing integration service platform is constructed and an agricultural product production and marketing integration service is realized so that an agricultural production level can be effectively increased and the agriculture performs a great effect in GDP growth, and simultaneously an income level of agricultural population is improved, acceptance of labor forces is increased and new rural construction is promoted.

The invention discloses a method for producing Nemadectin through fermenting. In the Nemadectin production process through fermenting, carbohydrates are intermittently or continuously added to a fermentation liquid to maintain the total sugar concentration of the fermentation liquid at 10-40g / L in order to carry out fermenting production when the total sugar concentration of the fermentation liquid is less than 30g / L. The method enables the Nemadectin fermentation unit to be improved by above 50%, simultaneously has the advantages of simple production operation, low equipment requirements and low cost, and is a technology suitable for the industrialized production.

The present invention discloses a method for using pennisetum hydridum lignin for preparation of active carbon and application of the active carbon, the method comprises the following steps: hydrothermal coke can be prepared from the pennisetum hydridum lignin as a raw material by a hydrothermal method, the hydrothermal coke is impregnated with a solution of phosphoric acid, then the impregnated hydrothermal coke is put into a muffle furnace for carbonization, and finally the impregnated hydrothermal after the carbonization is activated by a hydrochloric acid solution, washed and dried to obtain the active carbon. The active carbon prepared from a pennisetum hydridum waste residue is just suitable for detoxification and decoloration of a hydrolyzate after pennisetum hydridum hydrolysis due to the pore size of the active carbon, and compared with the decolorization effect of purchased commodity active carbon, the decolorization effect of the active carbon is better.

The invention provides a process for preparing squalene by taking lignocellulosic agricultural and forestry wastes as raw materials, which aims at problems in the aspect of the production of squaleneby microbial fermentation in the prior art. The process comprises the steps of pre-treatment, saccharification, yeast-like fungi fermentation and squalene extraction and the like. According to the process provided by the invention, lignocellulose biological saccharification is combined with yeast-like fungi high-density fermentation; by adopting lignocellulose biomass as raw material, on the one hand, the cost of enzyme usage and the production cost of squalene microbial fermentation in a saccharification stage are significantly reduced; on the other hand, the problem of comprehensive utilization of the agricultural and forestry wastes is solved. In addition, medium and fermentation medium in the lignocellulose saccharification stage can be recycled, water can be saved and usage of chemicals can be reduced, and has significant effect on reducing the discharge of wastewater and reducing the cost. In the process provided by the invention, the squalene yield can reach 13 g / L, accounting for 5% of the dry weight of thallus, and the purity of refined squalene is higher than 93%.

The invention relates to compound feed for promoting onset of estrus of replacement gilts. According to the technical scheme, the compound feed is prepared from raw materials in percentage by weight as follows: 5%-20% of wheat bran, 25%-55% of corn, 1%-15% of corn gluten, 1%-10% of corn DDGS (distillers dried grains with solubles), 5%-35% of glucose, 3%-15% of soybean meal, 1%-2% of stone powder, 1%-2% of calcium hydrophosphate, 0.1%-3% of corn oil, 0.1%-0.5% of table salt, 0.1%-0.3% of amino acid, 0.2%-0.6% of compound trace elements and 0.3%-0.4% of multivitamins. The compound feed is applied to promotion of onset of estrus of the replacement gilts, can effectively regulate and control secretion of insulin, glucagon and progesterone, shorten the age of puberty of the replacement gilts, increase the feed intake of the replacement gilts and increase the number born alive and the litter weight of gilts, and has important significance in improving productivity of the gilts.

The invention provides a technology for producing astaxanthin through efficient saccharifying of lignocellulose type agricultural and forest wastes, aiming at the problems in the aspects of producingthe astaxanthin by fermenting through phaffia rhodozyma and carrying out enzymolysis on lignocellulose. The technology comprises the steps of pre-treating, saccharifying, carrying out phaffia rhodozyma fermentation, extracting the astaxanthin and the like. According to the technology provided by the invention, a lignocellulose biomass is used as a raw material, so that on one hand, the enzyme utilization cost of a saccharifying phase and a production cost of microorganism fermentation are remarkably improved; on the other hand, the comprehensive utilization problems of the agricultural and forest wastes are also solved. By combining biological saccharifying of the lignocellulose and a high-density fermentation phase of the phaffia rhodozyma, ash obtained by saccharifying can be further used as a solid matrix of yeast fermentation and the fermentation density is improved. In the technology provided by the invention, the ratio of the astaxanthin to the dry weight of thalli can reach 2.6percent and the biomass of yeast thalli can reach 65g / L. The purity of the obtained refined astaxanthin is greater than 90 percent through technologies including molecular distillation and the like.

The invention discloses a fixed bed catalyst preparation method and a fixed bed catalyst. The method comprises that a molded carrier contacts a metal compound solution in an impregnation machine, and the molded carrier after contacting is dried and calcined, wherein the impregnation machine comprises an obliquely-arranged roller (10), the roller (10) comprises a rotatable cylinder (1) and a spray device (2), the inner wall of the cylinder (1) is provided with a spiral blade, and the spray device (2) is fixedly arranged and extends into the internal space of the cylinder (1). According to the method, a molded carrier is conveyed into the cylinder (1), and a metal compound solution is sprayed on the molded carrier with the spray device (2). With the method, the fixed bed catalyst with characteristics of good impregnation uniformity and low impregnation breaking rate can be obtained, automatic catalyst impregnation control can be achieved, production cost can be reduced, and requirements of large-scale industrial production can be met.

The invention relates to an industrial three-dimensional production system for modern agriculture, which comprises a plurality of elementary planting and breeding units formed by a three-dimensional combined frame, wherein columns (2) at four corners of the three-dimensional combined frame (1) are water supplementing columns which supplement water for the planting and breeding units, two columns (2) on the opposite sides are electrified columns to provide power for the planting and breeding units; a base frame (11) are fixed with the columns (2) to serve as the base of the frame; and the production system is provided with a water, optical and electric integrated intelligent platform (29) and a planting and breeding system, wherein the water, optical and electric integrated intelligent platform (29) provides light, water and power needed by growth for the planting and breeding system. The planting and breeding system comprises planting discs and culture boxes. The planting discs are placed in the elementary planting and breeding units. The culture boxes are placed on the lower part of the base frame (11) to form an integral planting and culturing three-dimensional production system. Agricultural production is assembled to an integrated modularized system so that the industrial three-dimensional production system for modern agriculture can be efficiently applied to an agricultural biological circulating economical system which is energy-saving.

The invention provides a floating type water quality detection device in an aquaculture Internet of Things online monitoring system. The floating type water quality detection device comprises a floating shell, a sealing cover, a port sealing piece, a supporting seat, a solar power generation plate, a storage battery, an integrated circuit board, a detection sensor group and a signal transmitter, wherein the storage battery, the integrated circuit board and the signal transmitter are arranged in the floating shell; the detection sensor group for detecting temperatures, dissolved oxygen and pH (Potential of Hydrogen) values are hermetically mounted at the bottom of the floating shell; the sealing cover is hermetically mounted at the upper port of the floating shell; the lower end face of the supporting seat is fixedly arranged on the sealing cover and the solar power generation plate is mounted on the upper end face of the supporting seat; the solar power generation plate is electrically connected with the storage battery; the storage battery supplies power to the integrated circuit board, the detection sensor group and the signal transmitter. The floating type water quality detection device can be used for carrying out real-time synchronous detection on the water temperatures, the dissolved oxygen and the pH values of a pond; an external electric supply is not needed and an electric wire does not need to be erected in the pond; the floating type water quality detection device is one of key equipment in the aquaculture Internet of Things online monitoring system.

The present invention relates to novel polynucleotide sequences comprising genes that encode novel lipolytic enzymes, as well asfunctional equivalents of the gene or the amino acid sequences with high homology thereto. The invention also relates to methods of using these lipolytic enzymes in industrial processes, for example in the dairy or baking industry.

The invention discloses a south high moisture and hot region beef cattle rapid fattening culture method comprising four stages: beef cattle rapid fattening early stage preparation; beef cattle rapid fattening adaptation stage; beef cattle rapid fattening transition period; beef cattle rapid fattening later stage; the method also comprises a beef cattle cooling process, a beef cattle dermatopathy prevention process and a beef cattle enterogastritis prevention process. The method employs store cattle of 12-18 months, preferably uses castrated bulls, and the poise characteristics comprise the following: 300-400kg body weight, healthy growth, big physique, strong arms and legs, and anosis. The beef cattle cultured by the method can have fullness body muscles after fattening, the fur is bright, and average daily weight growth is obvious.

An embodiment of the invention provides a two-year-triple-cropping high-yield cultivating method of winter wheat and summer corn and relates to the field of agriculture. The two-year-triple-cropping high-yield cultivating method includes that high-quality varieties of wheat and corn which are golden wheat 26 and Lingyu No.4 respectively are selected; the growth period of the two varieties is 343 days in total, solar heat resources are sufficiently utilized, multiple cropping in normal does not miss the farming season; the summer corn with the green is watered in the milk-ripe stage; in case of years with low-temperature corn seasons, sowing time of the golden wheat 26 ranges from October 5th to October 20th on the basis of increase of seeding rate, the lowest accumulated temperature before the winter is ensured to be 580 DEG C, and the accumulated temperature of 180-220 DEG C is given to enable the Lingyu No.4 to be grouted and ripened sufficiently; when content of rapidly available phosphorus in the soil is higher than 25milligrams / kilogram, phosphatic fertilizer is centrally applied to the golden wheat 26 all year round; the Lingyu No.4 is mechanically and directly sowed in double seeds and wide distance on a hard land with wheat stubble without tillage; straws directly return to the field. By the two-year-triple-cropping high-yield cultivating method, resource productivity is improved, and food production level of Tianjin city is enhanced.

The invention discloses an efficient feed additive and a preparation method thereof and belongs to the field of feed additives. The feed additive comprises the following raw materials in parts by weight: 10-15 parts of a perillae extract, 5-10 parts of a yeast cytoderm extract, 15-30 parts of a bacillus subtilis culture, 5-8 parts of an astragalus membranaceus extract, 3-6 parts of a codonopsis pilosula extract and 2-6 parts of fructo-oligosaccharide. The feed additive is small in use amount, stable in performance and safe to use; the astragalus membranaceus extract, the codonopsis pilosula extract and the perillae extract are reasonably matched with the bacillus subtilis culture and the extraction rates of beneficial components including amino acids, polysaccharides, microelements and the like in Chinese herbal medicines are increased; bacillus subtilis is used for promoting the growth of beneficial anaerobic bacteria, reducing the PH (Potential of Hydrogen) value of intestinal tracts and improving intestinal flora; the digestion and the absorption of the beneficial components in Chinese herbal medicines in a feed are promoted effectively; the production performance and the stress performance of livestock and poultry are enhanced and the comprehensive benefits of feeding are increased; the daily gain of animals and the feed conversion rate are improved and the usage amount of the feed is saved.

The present invention provides a method for artificially developing novel brassica napus. The excellent characters of cabbage and mustard type rape are integrated for obtaining novel brassica napus. The hybridization compatibility when the mustard type rape and cabbage are used for executing interspecific hybridization is higher than the hybridization compatibility when the Chinese cabbage and cabbage are used for executing interspecific hybridization. The interspecific hybrid is easily obtained, and simultaneously, the advantages of drought resistance, cold resistance, excellent strain type, etc. of the mustard type rape can be introduced into the brassica napus. The method of the invention also has the advantages of stable heredity, high speed, large area of character variation and facilitation for selecting the brassica napus with excellent charcter. Furthermore, the chromosomes of AC chromosome set of brassica napus obtained from the method of the invention respectively come from the mustard type rape and cabbage, have larger difference from the prior brassica napus. The use of hybrid vigor of genome of brassica napus is facilitated and the production level of cabbage is increased.

The invention provides a plugging control and drainage assisting double-effect auxiliary thick oil huff-puff development method. The method includes the following steps that a solid-phase particle water plugging slug is injected into a thick oil well to plug an oil layer; when the injection pressure is increased to 1.0 MPa or above, a foam plug and a nitrogen plug are sequentially injected into the oil well to plug a high-permeability hole channel in the oil layer; when the injection amount of the foam plug reaches the design amount, the foam plug stops being injected, and only the nitrogen plug is injected into the oil well; when the injection amount of nitrogen reaches the design amount, a sealing plug is injected into the oil well to seal the injected nitrogen and foam in the oil layer, and finally steam injection huff-puff production is carried out. By means of the method, stratum energy can be effectively supplemented, gas channeling in the construction process is avoided, an oil layer steam absorption section is adjusted, the swept volume is increased, and the purposes of improving the high-round low-efficiency steam huff-puff well, restoring capacity, increasing the recovery ratio of a thick oil reservoir, prolonging the production period, improving the production level and increasing the oil-steam ratio are achieved.

The invention discloses a deep learning-based tire defect intelligent detection method. According to the method, based on an original X-ray detection device, an original video is divided into two channels through a video distributor; one channel is used for an operator to perform manual judgment, and the other channel is used for collecting an X-ray image through a high-speed video collection cardand transmitting the X-ray image to an identification system; and automation is realized by utilizing an artificial intelligence technology, so that the productivity is improved and the labor cost isreduced.

The present invention is heavy ion beam irradiation effect to obtain quinhydroxy ketone, and relates to heavy ion beam irradiation technology. Heavy ion beam irradiation process for developing new medicine is superior to available chemical synthesis process, which has long developing period, high cost and high manpower consumption, and can obtain new matter in short period and separate out lead compound with obvious medicine effect. The heavy ion beam irradiation process of obtaining quinhydroxy ketone is to irradiate quinalkenyl ketone with 16O8+ and 12C6+ heavy ions in the initial energy of 75 MeV / micron, and energy depositions of 1.585x10<2> Gy-1.585x10<12>Gy and 1.656x10 Gy-1.656x10<9>Gy. The irradiation product quinhydroxy ketone has strengthened antibacterial activity and lowered toxicity, and the present invention provides one effective way for developing new medicines.

The invention provides a method for increasing hybrid rape planting purity by applying a herbicide resistant mutant character, which belongs to a plant breeding method. A conventional breeding technique is applied to transfer and breed imidazoline ketone herbicide resistance to a cytoplasmic male sterility restorer; a resistance restorer is utilized to selectively breed new combination of hybrid rape; and when the new combination F1 is planted, an imidazoline ketone herbicide is sprayed once at the seedling stage, so that all false hybrids without resistance such as trace pollen selfing plants, foreign pollen pollution plants and all mechanical mixing plants in a sterile line can be killed and removed, thereby greatly increasing the hybrid rape planting purity and maximizing the potential yield increase and the yield level of hybrids.

A diaphragm article includes an elastomer body reinforced with a pulp dispersion of polyaramid fibers. The elastomer has a material composition consisting of a silicon and / or fluoro-silicon polymer. The elastomer is blended with fiber to produce a heterogenous mixture. A compression molding process is used to fabricate the diaphragm, where a composition of aramid reinforced elastomer is employed as the preform structure. The diaphragm structure provides an increased strength and stiffness threshold. One diaphragm form includes fibers oriented generally orthogonally to the central pressure axis to provide improved radial stiffness. The diaphragm construction may be augmented with fabric reinforcement to provide further improvements in durability and performance.

The invention discloses a one-hill-multiple-plant maize high-yield culture technique which changes the traditional maize micro-ridge single-plant seed-clearing culture mode and adopts the combined culture manner of one-ridge-plant one-ridge-empty (one plant to empty plant) and one-hill-multiple-plant, and the row spacing is 100-110cm and the hill spacing is 50-60cm. In the culture technique, the root systems of the multiple plants in one hill densely grow together, the root quantity is great, the root is deep, the range of the root system is large, plus the inter-plant spatial distance in hills is greatly expanded, the wind blows through wider space and the resistance of the plants to the wind becomes weak, thus greatly improving the resistance to lodging of the maize. The expansion of the inter-plant spatial distance in hills contributes to fine light transmission and photosynthesis enhancement, so that the maize plants can grow and develop in the most suitable environment, thus effectively increasing the per mu yield of the maize.