159 results about "Streptomyces strain" patented technology

The invention discloses a streptomyces corchorusii strain NF0919, which is collected in China General Microbiological Culture Collection Center, and has the collection number CGMCC No. 3968. The strain can be used for preventing and treating rhizoctonia cerealis, rice false smut, rice sheath blight disease and other various plant diseases.

The invention pertains to the technical field of biology and medicine, and relates to a bacterial strain of streptomyces fradiae NTGA-334 with a preseravation no. of CGMCC No.2074, and a process for using the bacterial strain to ferment and transform Compound K in glycol group in various notoginsenosides so as to prepare 20-O-beta-D-glucopyranoside-20 (S)-protopanaxadiol. The culture media are Gause's no.1, ISP2, corroding acid (HSG culture medium) and asparagine culture medium. During the fermentation, the dosing proportions of various notoginsenosides are 0.1-10% (w/v), fermenting temperature is 18-50 DEG C, dynamically adjusted pH value of 0.1-35%(w/v) aqueous ammonia is 0.1-30% (w/v). The invention transforms and ferments in a scaled way various notoginsenosides, prepares Compound K, provides a streptomycete stain that is of steady condition, simple nutrition requirement and good growth, and provides a process for fermenting and separating by a simple and easy fully automatic fermenting tank. Using this bacterial strain and process to produce Compound K, the production cost is low, the foreign matter is less, and the yield rate is high.

The invention belongs to the technical field of biological pesticides, discloses a Streptomyces l avendulae xjy strain which has been collected in the general microorganism center of CCCCM in 8.16.2007 with the collection number: CCMCC No.2130. The invention also discloses a separation and authentication method for the strain, a preparation method for antibiotic active substances of the Streptomyces l avendulae xjy strain, authentication for analysis structures of the active substances, prevention and cure experiments for metabolic active substances of the xjy strain against leaf muld of tomato, powdery mildew of cucumber and phytophthora blight of pepper, and growth-promoting experiments for the growth of the strain. The antibiotic active substances secreted by Streptomyces xjy strain is mainly nucleosides antibiotic having the characteristics of persistent effect and no medicinal hazard as well as having the effects of obviously preventing and curing vegetable diseases and promoting the growth to a certain extent.

A novel antibiotic-producing Streptomyces sp. is provided that exhibits antifungal activity only on certain specific plant pathogens. Also provided is a method of treating or protecting plants from fungal infections comprising applying an effective amount of an antibiotic-producing Streptomyces sp. having all the identifying characteristics of NRRL Accession number B-30145. The invention also relates to fungicidal compositions comprising this novel Streptomyces strain and the antibiotics and metabolites produced by this strain either alone, or in combination with other chemical and biological pesticides.

The invention discloses a novel Streptomyces strain and application thereof. The strain is named as streptomyce sp. HS-HY-153, and the preservation number thereof is CGMCC NO. 14806; the invention also discloses a method for preparing Staurosporine by culturing the strain HS-HY-153.

The invention provides a marine Streptomyces strain and use thereof. The strain provided by the invention is Streptomyces sp. FXJ7.388, and the collection number of the strain is CGMCC No.4474. The invention also provides the use of the strain in the preparation of an antibacterial product. The antibacterial effect is to inhibit gram-positive bacteria, including Multi-resistant Staphylococcus aureus (MRSA 1-1), staphylococcus aureus CGMCC1.2386, bacillus subtilisGCMCC1.2428 and/or Candida albicans 2.538. Experiments prove that the Streptomyces sp. FXJ7.388 separated by the invention has the advantage of resisting gram-positive bacteria and Candida albicans, namely having high activity for resisting MRSA, staphylococcus aureus AND bacillus subtilis and certain inhibiting effect on Candida albicans.

The invention discloses wettable powder of a streptomyces roseoflavus biological bactericide, which adopts diatomite as a matrix and comprises the following components: not less than 108 propagules of streptomyces roseoflavus strain 0116, and 4% of a wetting dispersant. It is determined that diatomite is used as a carrier and sodium lignosulphonate is used as the wetting dispersant. In addition, the strain wettable powder storage stability is determined by regular detection of the viable bacterial count, and results show that after strain wettable powder storage for 24 months, the viable bacterial count is more than 80%. The invention has the characteristics of short development period, low cost, simple process, and capability of being developed into various dosage forms, and is suitable for large-scale production. Since propagules of streptomyces roseoflavus strain 0116 is used as the main component of the wettable powder, the invention is suitable for large-scale production, and when compared with strain fermentation liquor, the invention greatly shortens the field using amount, and reduces packaging and transport cost.

The invention relates to a strain for generating phospholipase D with high and stable yield by utilizing physical and chemical mutation, belonging to the technical field of microbial mutation. The strain is characterized in that the phospholipase D is prepared by fermenting streptomyces chromofuscus of high yield phospholipase D, which is obtained by taking the streptomyces chromofuscus as an original strain and sequentially carrying out composite mutation of ultraviolet rays and lithium chloride and composite mutation of atmosphere cold plasmas and the lithium chloride on a monospore bacterial suspension thereof. The invention has the advantages that the streptomyces chromofuscus of the high yield phospholipase D is obtained through mutation screening, thereby providing a high yield strain with higher application value to industrial production of the phospholipase D, laying a good foundation for fermentation culture and application of the phospholipase D, and also providing a good and feasible method for microbial mutation breeding. The invention adopts a mutation and screening method, has the advantages of simple operation, high efficiency and the like, and has a practical value on screening the strain with an industrial application value.

A streptomyces strain, and anti-kidney-cancer applications of piericidin metabolites thereof are disclosed. The streptomyces strain is streptomyces sp.HBERC-58855, and is deposited in the China Center for Type Culture Collection, in Wuhan University, Wuhan, China on April 20, 2017. The accession number of the streptomyces sp.HBERC-58855 is CCTCC NO:M 2017186. The 16sRNA nucleotide sequence of the streptomyces sp.HBERC-58855 is shown as SEQ ID NO:1. Piericidin compounds including piericidin A, piericidin A2, piericidin C2, IT-143-A, IT-143-B and piericidin s3a are extracted from the streptomyces sp.HBERC-58855 through fermentation for the first time, and are applied in preparation of anti-kidney-cancer medicines. Candidate compounds are provided for development of novel anti-kidney-cancer medicines. The streptomyces and the piericidin compounds are of great significance for marine microorganism medicine resource development.

The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.

The invention discloses Streptomyces griseofuscus LS-H1, namely CCTCC M 209211, and a method for preparing epsilon-polylysine and salt thereof by utilizing the strain. The Streptomyces griseofuscus LS-H1 is the strain obtained through screening; and the epsilon-polylysine at the concentration of between 0.7 and 20g/L can be accumulated under optimum conditions by culturing the Streptomyces griseofuscus LS-H1 in a culture medium containing a carbon source and a nitrogen source. After thalluses are removed from a zymotic fluid through centrifugation or filtration, the epsilon-polylysine and the salt thereof are obtained through the separation by an ion exchange method. The method is simple in the culture of the thalluses, and is favorable for industrialized mass production.

Aiming at the existing defects in preventing and controlling black shank of tobacco, the invention provides Streptomyces misawanensis D35 for inhibiting Phytophthora parasitica var. nicotianae. The strain was collected in China General Microbiological Culture Collection Centre (CGMCC) on August 27, 2009 with the collection number of CGMCC No: 3250. The invention also provides application of the strain in effectively inhibiting diffusion of Phytophthora parasitica var. nicotianae in tobacco, reducing disease incidence, improving the tobacco yield and quality, facilitating the development of biological prevention and control products and further promoting the safe control of Phytophthora parasitica var. nicotianae.

The invention discloses a nonabsorbent streptomyces strain BWL58 for preventing and treating a banana vascular wilt. The strain BWL58 is classified and named as nonabsorbent streptomyces ahygroscopicus, and is preserved in the China General Microbiological Culture Collection Center on May 9, 2011; the preservation number is CGMCC No. 4837; the strain has strong antagonistic capability to the pathogenic bacteria of the banana vascular wilt and can be used for preventing and treating the banana vascular wilt; the invention further discloses a microbial inoculum containing the strain, a preparation method and application thereof; and by using the microbial inoculum, not only can the banana vascular wilt be inhibited, but also the microbial inoculum has a certain growth promotion effect on a plant.

A streptomyces globisporus H50 (CCTCC No.M203034) for preventing and eliminating the hypochnus and blast of rice is obtained from soil by screening with PAD and YPTG plate method. Its advantage is high effect (more than 65% for its bacterium inhibiting rate).

The invention belongs to the field of biotechnology and agricultural production, especially relates to a Streptomyces termitum ACT-2 strain, its culture and application. Streptomyces termitum ACT-2 strain is preserved in China Center for Type Culture Collection (CCTCC); the address is College of Life Sciences, Wuhan University, Luojia Mountain, Wuchang District, Wuhan, Hubei Province; the collection number is CCTCCCNO: M2011008 and the collection date is Jan. 7, 2010. Streptomyces termitum ACT-2 strain, which has an effect of resisting Xanthomonas oryzae pv. oryzae, is obtained by separating and screening from paddy soil. The strain and its fermented products can be used as a biopesticide and also used to control Xanthomonas oryzae pv. oryzae and the like.

The invention provides a genetic engineering bacterium capable of promoting the synthesis of antibiotic medermycin and a method for producing the medermycin by using the same. The method comprises the steps that by taking deoxyribonucleic acid (DNA) of a genome of Streptomyces nashvillensis AM-7161 as a formwork and utilizing degenerate primers to conduct polymerase chain reaction (PCR) amplification, a new complete sequence of genes of ribosome recycling factors (RRFs) of the Streptomyces is obtained; the genes are downstream placed on an efficient promoter PtipA of the Streptomyces, so that a plasmid pHSL56.2 capable of efficently expressed in the Streptomyces is constructed; the pHSL56.2 is led into the host cell Streptomyces AM71-61, and then an engineering bacterium AM71-61/pHSL56.2 (with a preservation number of CCTCCNo:M2012093) is obtained; and when the engineering bacterium AM71-61/pHSL56.2 is used to conduct solid fermentation and liquid fermentation, the accumulation of the antibiotic medermycin can be effectively promoted. The efficient expression plasmid pHSL56.2 of the genetic engineering bacterium can also be directly led into a Streptomyces strain cell for producing other antibiotics of a benzoisochromanequinones family or other aromatic polyketide antibiotics, so as to obtain corresponding antibiotic high-yield engineering bacteria. By utilizing the method, the antibiotic high-yield bacteria can be genetically bred, so that the synthetic capability of the antibiotics is enhanced.

The invention discloses streptomyces strains and joint application thereof in prevention and treatment of cucumber fusarium wilt and relates to the technical field of microbes. Actinomycetes disclosed by the invention are obtained by separation from soil samples collected from rhizospheres of plants in Xixi Wetland, Zhejiang by adopting an actinomycetes separation technology, identified by microbial taxonomy and named as Streptomycesalbus 2013 and Streptomycestenebrarius M929, wherein S. albus 2013 was collected in China Center for Type Culture Collection on June 19, 2013 with the collection number of CCTCC NO: M2013269; and S. tenebrarius M929 was collected in China Center for Type Culture Collection on December 25, 2013 with the collection number of CCTCC NO: M2013710. The two strains can be fermented to produce active substances with relatively strong antagonistic effect against the cucumber fusarium wilt (Fusariumoxysporumf. sp. cucumebrium) and can be used for preventing and treating the cucumber fusarium wilt. When fermentation products of the two strains are mixed according to the ratio in percentage by weight of (3:2)-(1:1) to prepare a mixed preparation, the effect of preventing and treating pathogenic bacteria of the cucumber fusarium wilt is more significant than that when each of the two strains is used as a single preparation respectively.

A method of vanillin production from ferulic acid with a high concentration by biotransformation using a Streptomyces sp. strain is claimed in this invention. This strain is named as Streptomyces sp. V-1, which has been deposited in China Center for Type Culture Collection on Jul. 12, 2006 with the number of CCTCC M 206065. Using this strain, high concentration of vanillin fermentation broth is obtained from ferulic acid by biotransformation in GY biotransformation medium. With the addition of macroporous adsorbent resin DM11, the concentration of vanillin can be greatly improved. The advantage of this invention is less environmental pollution, high product concentration, less by-product, short processing cycle, low production cost, simple product extraction, clean production process, product environmental friendly, safe and reliable, which solves many difficulties in the vanillin production from botanical raw material extraction or chemical synthesis, and therefore it has good application prospect.

The invention discloses a preparation method for a dichloro substituted II-type halogenated polyketone compound zunyimycin A, wherein the compound is derived from a secondary metabolite of a streptomyces sp.FJS31-2 strain (with the strain preservation number of CGMCC4.7321), and finally, a structure is identified and determined after strain activation, expansion culture and isolation. The compoundcan be used in treatment of diseases infected by gram-positive bacteria such as staphylococcus aureus (MRSA) and drug-resistant bacteria thereof, staphylococcus epidermidis, bacillus subtilis and thelike, is used in treatment of diseases infected by gram-negative bacteria such as super-spectrum beta-lactamase escherichia coli (ESBL), proteusbacillus vulgaris, bacterium burgeri and the like, andis used for treatment of diseases infected by fungi, such as candida albicans.

The present invention relates to a new strain of Streptomyces sp., called CIMAP A1 isolated from the soil of geranium (Pelargonium graleoleus) planted in the experimental fields of CIMAP and having the accession No. . . . and capable of inhibiting the growth of phytopathogenic fungi.

The invention discloses a streptomyces strain and a combined application thereof in the prevention and treatment of tomato blight and relates to the technical field of microbes. According to the streptomyces strain and the combined application thereof, actinomycetes are obtained through collecting 10-15cm deep-layer soil samples from saline-alkaline land of a suburban district of Tai'an in Shandong and separating by adopting an actinomycete separation technology, and are named streptomyces rimosus M527 and streptomyces globisporus M927 through microbial taxonomic identification, S. rimosus M527 has been collected in CCTCC (China Center for Type Culture Collection) on June 19, 2013 and has the collection number of CCTCC NO: M 2013270, and S. globisporus M927 has been collected in CCTCC on December 25, 2013 and has the collection number of CCTCC NO: M 2013709. Both strains can be fermented to generate active substances which have relatively strong antagonism on Fusarium oxysporum f. sp. Lycopersici Snyder et Hansen and can be used for preventing and treating plant diseases, such as tomato blight. When fermentation products of the two strains are mixed according to the weight percent of (4: 1) to (7: 3), the biological prevention effect is particularly remarkable compared with that when the two kinds of metabolic products are used respectively as single preparations.

The invention discloses a method for increasing the yield of lincomycin by co-expressing metK1 and metK2 genes in streptomyces lincolnensis. The method is characterized in that the metK1 and metK2 genes in the streptomyces lincolnensis are co-expressed through a genetic engineering way, so that an engineering strain with high lincomycin yield of the streptomyces lincolnensis is obtained; and the strain obtained by the technology can be fermented to increase the yield of the lincomycin.

The invention discloses a bacterial-wilt-resistant antagonistic bacterium strain. The strain is named Streptomyces rochei L-9, and was collected by China General Microbiological Culture Collection Center on September 25th, 2013; and the collection number is CGMCC No.8264. The strain L-9 has high bacterial wilt resistance, and the antagonistic ring radius reaches 15mm. The invention provides a new microbial resource for biological control on bacterial wilt, and overcomes the defects in the prior art.

The invention relates to the fermenting method for producing natamycin that breeds a streptomycete strain of high yield natamycin, by the method of fermenting metabolizing method to select the fermenting culture medium compounding and fermenting technology condition. The method has the advantages of high yield, low cost, short producing cycle, etc.

The invention discloses a preparation method of a monensin premix. The preparation method comprises the following specific steps: preparing cinnamon streptomyce strain; seed flask culture and seed tank culture are performed; when the age of the seed is 20-24h, the pH of a culture solution is 6.6-7.1, the biomass is above 10%, and microscopic examination shows that mycelia are thick and reticular, are stretched and darkly stained and are free of foreign bacteria, performing fermentation culture is performed; when the residual oil amount is below 0.7%, the biomass is above 40%, the mycelium concentration is 40-50%, and the monensin titer is not lower than 40000U/mL, fermentation is finished; monensin is extracted by a one-step spray drying method, wherein the prepared finished monensin product has the monensin content of above 20% and the grain rate of above 75%. According to the preparation method, the fermentation process is simple, so that the fermentation unit can be effectively increased and the production cost can be reduced; usages of an animal-derived nitrogen source and a chemical organic solvent are avoided.