Streptomyces termitum ACT-2 strain, its culture and application

A technology of Streptomyces termites and ACT-2, which is applied in the field of biotechnology agricultural production and can solve the problems of difficulty in meeting biological control requirements and low bacteriostatic potency.

Inactive Publication Date: 2012-02-29
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the microorganisms that have been reported to have antagonistic effects on bacterial blight are mainly bacteria, such as Bacillus subtilis and Enterobacter, and their antibacterial potency is low, which is difficult to meet the biological control requirements of agricultural production. Actinomycetes active against Leaf blight have rarely been reported

Method used

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  • Streptomyces termitum ACT-2 strain, its culture and application
  • Streptomyces termitum ACT-2 strain, its culture and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] An antagonistic strain of rice bacterial blight Streptomyces termites ( Streptomyces termitum ) ACT-2 strain.

[0026] (1) Medium

[0027] Gaoshi No. 1 liquid medium: 20 g soluble starch, KNO 3 1 g, K 2 HPO 4 0.5 g, MgSO 4 0.5 g, NaCl 0.5 g, FeSO 4 0.01 g, distilled water 1000 ml, pH 7.2-7.5.

[0028] Beef extract peptone solid medium: 3 g beef extract, 10 g peptone, 5 g NaCl, 20 g agar, 1000 ml water, pH 7.0-7.2.

[0029] (2) Method

[0030] A gradient dilution separation method was used. Take 1 g of soil and add 9 ml of distilled water for gradient dilution (10 -1 -10 -4 ), choose the appropriate concentration (10 -2 ) 100 μl spread on Gaussia No. 1 medium plate, cultured at 28 ℃ for 24 h, and a round white dry colony appeared, which can be preliminarily determined that the strain is an actinomycete. A single colony is picked, and after further separation and purification, the ability of the isolated and purified strain to antagonize bacterial blig...

Embodiment 2

[0035] Streptomyces termites that have antagonistic effects on rice bacterial blight ( Streptomyces termitum ) The preparation method of ACT-2 bacterial strain fermented liquor.

[0036] (1) Strain: ACT-2 strain.

[0037] (2) Method

[0038] After the bacteria were activated by Gaoshi No. 1 solid medium, they were mixed with sterile water to make 1×10 6 1 / ml of the spore suspension, take 1 ml into Gaoshi No. 1 culture medium, activate and culture at 28 ℃ for 24 h; put 50 ml Gaoshi No. 1 culture medium into a 250 ml Erlenmeyer flask, and use 2% inoculation amount, 28 ℃, 150 r / min, shake flask culture for 48 hours, and obtain the fermentation broth with antagonistic activity against bacterial blight, and the antibacterial effect was determined by the Oxford cup method, and the diameter of the inhibition zone was 21.73 mm.

[0039] After preliminary optimization of fermentation conditions, when 50 ml of Gao Shi No. 1 culture medium was filled in a 250 ml Erlenmeyer flask, the...

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Abstract

The invention belongs to the field of biotechnology and agricultural production, especially relates to a Streptomyces termitum ACT-2 strain, its culture and application. Streptomyces termitum ACT-2 strain is preserved in China Center for Type Culture Collection (CCTCC); the address is College of Life Sciences, Wuhan University, Luojia Mountain, Wuchang District, Wuhan, Hubei Province; the collection number is CCTCCCNO: M2011008 and the collection date is Jan. 7, 2010. Streptomyces termitum ACT-2 strain, which has an effect of resisting Xanthomonas oryzae pv. oryzae, is obtained by separating and screening from paddy soil. The strain and its fermented products can be used as a biopesticide and also used to control Xanthomonas oryzae pv. oryzae and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology agricultural production, in particular to Streptomyces termites ( Streptomyces termitum ) ACT-2 strain and its culture and use. Background technique [0002] Actinomycetes widely exist in soil, air and water, most of which have the ability to produce antibiotics. Streptomyces produced 80% of the antibiotics produced by actinomycetes. Streptomyces play a very important role in the industrial production of antibiotics. [0003] The antibiotic substances produced by some Streptomyces can have specific antagonistic effects on some plant pathogenic bacteria, and can inhibit the growth and reproduction of pathogenic microorganisms. It has a certain degree of control effect on plant diseases caused by the pathogenic bacteria. [0004] Rice bacterial blight is caused by Xanthomonas oryzae ( X. oryzae ), which is seriously harmful to rice production. Screening Streptomyces that have a certain antagonist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/465
Inventor 蒋冬花宋迤明董夏梦
Owner ZHEJIANG NORMAL UNIVERSITY
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