Bacillus amyloliquefaciens L-1 for antagonizing pear disease and application thereof

A technology of Bacillus powdery and Bacillus subtilis, applied in the field of microorganisms, can solve the problems of high residues, pome fruit diseases, etc., and achieve the effects of strong specificity, maintaining ecological balance, and reducing the residues of pome fruit chemicals

Active Publication Date: 2017-10-20
FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: how to provide a microorganism that can effectively antagonize pear fruit diseases, so as to solve the problems of serious pear fruit diseases and high residues caused by chemical agent control.

Method used

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  • Bacillus amyloliquefaciens L-1 for antagonizing pear disease and application thereof
  • Bacillus amyloliquefaciens L-1 for antagonizing pear disease and application thereof
  • Bacillus amyloliquefaciens L-1 for antagonizing pear disease and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The isolation of embodiment 1 bacterial strain

[0026] In September 2015, the rhizosphere soil of pear trees was collected in Xingcheng City, Liaoning Province, and the bacteria in the soil were separated by the dilution plate method (Herr, 1959). Weigh 10 g of the dried soil sample and grind it thoroughly, transfer it to a Erlenmeyer flask containing 90 mL of sterile water after grinding, and fully shake at 120 r / min at 4 °C for 30 min. Dilute the soil sample suspension step by step in the ultra-clean workbench, draw 100uL of the diluted solution and evenly spread it on NBA (Nutrient Broth Agar) (3g beef extract, 5g peptone, 2.5g glucose, 20g agar powder, 1L water) medium cultured at 32°C for 1 day, and a single colony was picked and purified on NBA medium. After purification, 90 single colonies were transferred to new NBA medium and kept for future use.

Embodiment 2

[0027] Embodiment 2 Active strain screening

[0028] Adopt the plate confrontation method (Khamna et al., 2009) to measure the inhibitory activity of 90 strains of bacteria isolated to R. pear rot bacterium, concrete operation is as follows: pick respectively on PDA medium (potato dextrose Agar) (6g potato extract powder , 20g glucose, 20g agar powder, 1L distilled water) for 5 days, put the ringworm bacteria cake with a diameter of 6mm in the center of the PDA plate, punch holes on both sides of the pathogenic bacteria cake 3cm away, deduct the medium and inoculate it on the NBA medium Bacterial cakes to be screened that had been grown for 2 days on the tube were incubated at a constant temperature of 25°C. Each treatment was repeated 3 times, and the plate not inoculated with actinomycetes was used as the control. When the control colonies covered the plate, observe and record the diameter of the inhibition zone. The result is as figure 1 Shown: bacterial strain L-1 has si...

Embodiment 3

[0029] Example 3 Control Effect of Active Bacterial Strain L-1 on Common Fruit Postharvest Diseases of Apples and Pears

[0030] The antagonistic effect of L-1 on pear black spot, gray mold, brown rot, penicillium, and apple anthracnose and mold core diseases was determined by Oxford cup method. The L-1 strain was transferred into 100ml NB culture medium, and cultured at 28°C and 180r / min for 2 days. Inoculate the pathogenic bacteria cake to be tested in the center of the PDA plate, place sterilized Oxford cups on both sides, and add 200ul of L-1 fermentation broth to the Oxford cup, and add NB culture fluid without L-1 spores as a control, Incubate at a constant temperature of 25°C and repeat 3 times for each treatment. The growth diameter of the pathogenic bacteria was measured in the control dish, and the control effect was calculated by (control effect (%)=(1-treatment lesion diameter / control lesion diameter)*100). The results are shown in Table 1. Strain L-1 has signifi...

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Abstract

The invention discloses a strain of bacillus amyloliquefaciens L-1 for antagonizing pear disease, which belongs to the bacillus subtilis, and a preservation number is CGMCC No.14373. The research result displays that the bacillus amyloliquefaciens L-1 can effectively inhibit the development of pear ring rot disease, can cause pathogen mycelia malformation, and has obvious antagonistic activity on the pear ring rot disease as well as apple/pear common fungal diseases. The bacillus amyloliquefaciens L-1 can induce the active expression of fruit resistance-related enzymes(POD, CAT), reduces the accumulation of malonaldehyde in the fruits, and delays fruit aging; the bacillus amyloliquefaciens L-1 has no damage on the fruit quality, can increase the VC content in the fruits; and enables successful and rapid breeding in the wounds of the fruits; the bacterial strain is salt tolerant, the activity of a bioactive secondary metabolite is stable, and the bacterial strain has the characteristics of ultraviolet radiation resistance, acid and alkali resistance, and high temperature and metal ions resistance, and has large application potential.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of bacillus amyloliquefaciens and its application. Background technique [0002] my country is the world's largest pome fruit producer and exporter. In 2016, my country's total pome fruit output reached 19.3 million tons, accounting for 75% of the world's total output. The export volume has gradually surpassed the EU and Argentina to become the world's largest pome fruit exporter (Sun Pingping and Wang Wenhui, 2016); however, the production and management level of pear orchards in my country is backward (Zhang Shaoling, 2013), the occurrence of diseases is serious, pesticide residues exceed the standard (Wang Tianli, 2015), and the average fruit yield, quality and price are significantly lower than those in developed countries (Wang Wenhui et al. , 2014). With the increase of pear fruit production in our country, improving fruit quality, enhancing tree disease re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A23B7/155C12R1/07
CPCA01N63/00A23B7/155A01N63/10C12N1/205C12R2001/07
Inventor 孙平平崔建潮贾晓辉王文辉
Owner FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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