428results about How to "Broad antibacterial spectrum" patented technology

The invention relates to a bacillus amyloliquefaciens growing in a disease-preventing and growth-promoting plant and application thereof, relating to bacillus amyloliquefaciens and the application thereof. The bacillus amyloliquefaciens TF28 growing in the disease-preventing and growth-promoting plant belongs to bacillus, has the storage number of CGMCC No.4038 and the storage data of July 26, 2010. The application of the bacillus amyloliquefaciens TF28 comprises the following steps of: (1) preparing activated bacilli liquid; (2) inoculating the activated bacilli liquid in an NYD culture fluid to obtain a strain fermentation solution; and (3) soaking seeds in a dilution solution 50 times of the strain fermentation solution. The strain TF28 in the invention can be colonized, reproduced and transmitted in plants, can simultaneously generate two antibacterial substances, i.e. antimicrobial proteins and lipopeptide antibiotic and has the multiple efficacy of preventing diseases, stimulating growth, increasing the yield, improving the quality, widening an antibacterial spectrum, and the like. Rice seeds treated by the dilution solution 50 times of the fermentation solution of the strain TF28 reach a room-temperature bakana resistance rate over 84.6 percent.

The invention discloses a bacillus velezensis strain and application thereof in prevention and control of plant fungus diseases. The strain is a bacillus velezensis strain Bv01 with the accession number of CGMCC No. 12744. The bacillus velezensis strain Bv01 has strong inhibiting effects on mycelial growth of curvularia geniculata, pythium ultimum, sclerotinia rolfsii sacc, alternaria solani, fusarium garaminearum schwabe and fusarium oxysporum, and mycelial growth inhibition ratios are separately 85.17%, 91.54%, 90.78%, 78.14%, 75.69% and 86.67%; and the fermentation product of the bacillus velezensis strain Bv01 has obvious inhibition effect on the curvularia geniculate, the sclerotinia rolfsii sacc, the alternaria solani, the fusarium garaminearum schwabe and the fusarium oxysporum, andthe inhibition rates are separately 96.47%, 100.00%, 83.14%, 77.25% and 87.84%. The fermentation liquor of the bacillus velezensis strain Bv01 can effectively prevent and treat pythium aphanidermatum, scelerotium rolfsii and wheat scab. A fungicide which takes the fermentation liquor of the bacillus velezensis strain Bv01 as an active ingredient has high application value on agricultural production.

The invention discloses a trichoderma viride fungi strain and a method for preparing a bio-control fungicide thereof and application. The strain is named as trichoderma viride strain ZBS6 and collected in the CGMCC, and the collection number is CGMCC No.3538. The microbial fungicide is liquid or solid fungicide, and is prepared from the trichoderma viride ZBS6 strain. The ZBS6 strain and the fungicide thereof have wide bacteriostatic spectrum and good bacteriostatic effect, and have strong inhibiting effect on botrytis cirerea, rhizoctonia solani, rhizocto-nia cerealis, gaeumannomyces grami, marssonina coronaria, fusarium oxysporum and the like; and the fungicide can be used for control of botrytis cirerea, cucumber downy mildew, rhizocto-nia cerealis, wheat soil-borne disease and wheat powdery mildew, is efficient, non-toxic and simple to use, and does not cause environmental pollution.

The invention relates to bacillus amyloliquefaciens which has stronger phosphate solubilizing and plant growth promoting functions and has a better preventing and treating effect on various plant diseases. According to the bacillus amyloliquefaciens JCD-H-12 with the phosphate solubilizing, disease preventing and growth promoting functions, disclosed by the invention, the preservation number is CGMCC NO.11856, the preservation date is December 10, 2015, and multiple effects of solubilizing phosphate, preventing diseases, promoting the growth, increasing the yield and the quality, having a broad bacteriostatic spectrum and the like are obtained; a bacterial strain and inoculant of the bacterial strain are capable of effectively preventing and treating powdery mildew, tobacco black shank, botrytis blight, rice blast, cucumber fusarium wilt, tomato early blight, sweet pepper anthracnose, watermelon sclerotinia sclerotiorum, wheat scab, watermelon fusarium wilt, phytophthora capsici and the like; a broad-spectrum antibacterial effect is obtained, the effect is stable and durable, and the bacillus amyloliquefaciens can be developed into a bacterial fertilizer and microbial pesticide preparation which integrates phosphate solubilizing with disease preventing and treating.

The invention belongs to the technical field of agricultural biocontrol, and especially relates to a broad-spectrum disease resistance bacillus and an application thereof. The biocontrol bacillus is classified and named as Bacillus velezensis, and the preservation number is CGMCC. No.15766. The Bacillus velezensis has the advantages of broad antibacterial spectrum, strong antagonistic effect, longlasting period, and effectiveness in inhibition of the growth of crop pathogenic fungi, and can be used in the fields of agricultural biocontrol to prevent and control crop diseases caused by gray mold, blight, anthracnose, stem rot, seedling blight, stalk break, gibberellic disease and other fungi. The Bacillus velezensis can also be used for promoting the growth of crop seeds and seedlings andthe preservation and fresh-keeping of fruits and vegetables. The Bacillus velezensis has the characteristics of strong resistance to ultraviolet and drought, simple preparation method and low cost, isa biocontrol strain with excellent performance, and has good development and application prospects.

The present invention discloses a watermelon juice probiotic fermented beverage and a preparation method thereof. The beverage preparation method uses watermelons as raw materials, scientifically blends prebiotic factors, does not add any essences and coloring matters, mainly uses low-temperature processing technology such as ultrasonic wave cleaning, high-pressure pulsed electric field treatment, biological enzymolysis, vacuum degassing, etc. in the whole process, replaces the existing heat sterilization and enzyme blunting technology, maintains the color and luster, flavor and mouth feel of the natural watermelon juice to a maximum degree, enhances the non-biological stability and biological stability of the watermelon juice, reduces the addition of chemical additives, increases the juice squeezing rate and food safety of the watermelon juice, and has a probiotic live bacteria content of 7 multiplied by 10^11 to 8 multiplied by 10^11 cfu/ml. The watermelon juice probiotic fermented beverage is environmentally friendly, can be processed into probiotic solid beverage and quantitatively packaged beverage which have natural taste, are nutritious and health-care, are convenient to drink, and have longer shelf lives, opens up a new way for watermelon deep processing and industrial development, and can achieve better economic and social benefits.

The invention discloses application of streptomyces griseoflavus in biological prevention and control of plant diseases, particularly streptomyces griseoflavus NMG6-3-9 CGMCC No. 3441 in biological prevention and control of plant diseases. The provided strains have good antagonism effect on pathogenic bacteria of alfalfa root rot, can be used for the biological prevention and control of alfalfa root rot and other plant diseases, and have wide application potential in the field of biological prevention and control of plant diseases. The invention lays a foundation for the biological prevention and control of alfalfa root rot and further provides a scientific basis for the development and the application of a biological prevention actinomycete preparation.

Provided is a plant tissue culture bacteriostatic (antiseptic) agent containing botanical fungicide. The bacteriostatic (antiseptic) agent comprises isothiazolinone, nisin and aqueous extracts of the Chinese herbal medicines of terminalia, groundsel and aromatic madder. When the bacteriostatic (antiseptic) agent is applied in plant tissue culture, 0.35 to 0.5% of the bacteriostatic (antiseptic) agent is added into a medium, which enables fungal and bacterial contamination to a culture to be effectively reduced or prevented and poses little adverse influence on plant growth, thereby enhancing the success rate of plant tissue culture; the bacteriostatic (antiseptic) agent can also be applied in open type plant culture, e.g., in the rooting phase of sound seedlings during a late plant culture stage, the bacteriostatic (antiseptic) agent is directly added into a medium, and the medium can be inoculated in indoor environment with bacteria or a few bacteria without undergoing the step of autoclaving or operating on a superclean bench, thereby simplifying seedling culture in plant tissue culture, enhancing working efficiency, saving a great amount of energy and reducing production cost for plant tissue culture seedlings; therefore, the bacteriostatic (antiseptic) agent has a critical actual application value.

The invention relates to a composite natural fresh-keeping agent for cold fresh meat and application of composite natural fresh-keeping agent. The composite natural fresh-keeping agent for the cold fresh meat is characterized by comprising tea polyphenol, chitosan, nisin, natamycin, lysozyme, oregano essential oil, vitamin C, vitamin E, erythorbic acid, a spice extract and water in certain proportions by weight, wherein the spice extract is obtained by extracting ginger, garlic, cinnamon, cardamom, clove and rosemary in certain proportions by weight through ethanol extraction. The composite natural fresh-keeping agent for the cold fresh meat is prepared from biological-source raw materials by a simple preparation method, has the advantages of non-toxicity, safety, broad antibacterial spectrum and the like, is convenient to operate during using and prominent in fresh-keeping effect, and has an important industrial value.

The invention provides bacillus amyloliquefaciens HAB-2, which has the collection number of CCTCC M 2015070. Although the key lipopeptide synthetase gene sfp gene is deleted, the surfactin substances can still be generated, indicating that the strain has a specific and distinctive biosynthetic pathway and has a remarkably high research value. The bacillus amyloliquefaciens HAB-2 has very strong ability of inhibiting pathogenic fungi, has a wide antibacterial spectrum and can inhibit multiple kinds of pathogenic fungi while the antibacterial effect is good and the antibacterial rate is high. Further research finds that the active ingredient can be directly obtained through simple treatment on the fermentation broth of the strain, and the antibacterial active ingredient is stable and has very high activity in strong acid and strong alkaline environments; the antibacterial activity is not changed after 24 hours of ultraviolet irradiation, and high temperature does not influence the antibacterial active ingredient; and the fermentation broth of the biocontrol bacteria realizes certain growth promoting effect on a plant.

The invention discloses a probiotic foodstuff and a preparation method thereof. In the fermentation process of yoghourt, a functional starter, germinated brown rice, Chinese herbal medicine extract with anti-heat-and-cold irritability and a stabilizing agent capable of effectively enhancing stability are added; temperature-varying yoghurt fermentation enables propagation of lactic acid bacteria to be maximized and anti-heat-and-cold stress capability of lactic acid bacteria to be improved; non-thermal processing technology is employed for pretreatment of yoghurt and fruit and vegetable pulp so as to prevent infection with infectious microbes in preparation process, improve juice yield of the fruit and vegetable pulp, increase the content of soluble cellulose in the fruit and vegetable pulp and prevent browning of the fruit and vegetable pulp; and a scientifically compounded cryoprotectant with good anti-refrigeration effect is added during mixing of the fruit and vegetable pulp and the yoghurt. Eventually, the prepared probiotic foodstuff has fine mouthfeel, uniform texture, stable state, natural quality, a great number of live bacteria, strong functionality and long shelf life, wherein the content of the live bacteria is 1.75 * 10<11> to 2.55 * 10<11> CFU/g, and the shelf life is 30 to 36 months at normal temperature.

The invention relates to the technical field of biology, and specifically discloses a ginseng endophytic paenibacillus polymyxa and application thereof. The ginseng endophytic paenibacillus polymyxa was preserved in General Microorganism Center of China Microorganism Culture Preservation Management Committee on February 5, 2013. The address is No.3 No. 1 Court, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology Chinese Academy of Sciences. The preservation number is CGMCC No. 7250. The ginseng endophytic paenibacillus polymyxa is a paenibacillus polymyxa strain isolated and identified from a ginseng plant, can be widely and directly applied in plant disease control of ginseng, American ginseng, schisandra chinensis, fritillary bulb, and astragalus, can be applied in the preparation of preventions for treatment of diseases of ginseng, American ginseng and other plants, and can effectively solve the successive cropping obstacle problems of old ginseng grounds, and ginseng, American ginseng and other plants.

The invention relates to efficient bacillus subtilis for preventing and controlling plant fungal disease and promoting plant growth. The bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No.6788. The bacillus subtilis related by the invention has a high capacity of inhibiting the growth of plant pathogenic fungi, has high hereditary stability, does not pollute the environment, is ecologically safe, and can be widely applied to prevention and control of plant pathogenic fungi, especially Pythium aphanidermatum, Fusarium, Sclerotinia sclerotiorum and Botrytis cinerea. The bacillus subtilis is singly used or is used together with other organic and inorganic nutrients required in the growth of plants to be prepared into a biological compound fertilizer which has the advantages of remarkable continuous cropping resistance, premature senility resistance, and control of dead seedlings and rotten roots and is capable of ensuring correct color and large fruits, and increasing output and improving quality of improved products.

The invention belongs to the technical field of fresh keeping agents, aims to develop a composite fresh keeping agent including microorganism derived effective components and plant derived effective components, is used for fresh keeping and corrosion resistance for picked fruits and picked vegetables, and provides a microorganism and plant derived composite fresh keeping agent for fruits and vegetables. The composite fresh keeping agent is prepared from a component A and a component B, wherein the component A consists of microorganism fermentation fluid and a propolis extract, and microorganisms are bacillus amyloliquefaciens Lh-1, candida intermedia and cryptococcus laurentii; and the component B consists of plant derived effective component mixed liquor and film forming liquor, wherein the plant derived effective component mixed liquor is prepared by mixing a tree peony bark water extract with a citrus peel alcohol extract, a rabdosia rubescens alcohol extract, a radix zanthoxyli water extract, a lycium ruthenicum alcohol extract, a Chinese herbaceous peony water extract, a clinopodium chinense alcohol extract and a citronella water extract, and the film forming liquor is prepared by mixing konjac glucomannan with chitosan. Through the adoption of the composite fresh keeping agent, the fruits and the vegetables are furthest prevented from mildewing and rotting, the effects for preventing the fruits and the vegetables from postharvest diseases are enhanced, the moisture dissipation of the fruits and the vegetables is reduced, the storage time of the fruits and the vegetables is prolonged, and a certain economic value is created.

The invention provides Paenibacillus polymyxa (named as Paenibacillus polymyxa WK-3) with the collection number of CCTCC M2015298 and further provides a screening method of the Paenibacillus polymyxa WK-3, application of the Paenibacillus polymyxa WK-3 in inhibition of rice sheath blight, watermelon fusarium wilt and hyphal cluster root-rot sickness and a Paenibacillus polymyxa WK-3 bactericide. The Paenibacillus polymyxa WK-3 is an endogenous strain of rice, is safe to human bodies, livestock and crops, environment-friendly and relatively wide in antibacterial spectrum, has a relatively good antibacterial effect on the growth of mycelia of common soil-borne pathogens such as the rice sheath blight, the watermelon fusarium wilt and the hyphal cluster root-rot sickness, has the antibacterial rates of respectively more than 85.4%, more than 81.8% and more than 73.9% for the rice sheath blight, the watermelon fusarium wilt and the hyphal cluster root-rot sickness and has a remarkable effect of inhibiting the growth of mycelia of the rice sheath blight and generating mycelia malformation; the further prepared bactericide has a remarkable effect of preventing and controlling rice sheath blight; and the Paenibacillus polymyxa WK-3 is short in production period and has a very wide application prospect in biological prevention and control of plant diseases.

The invention discloses Pantoea agglomerans B50l CGMCC No.1655 and fermenting cultivating method and application. The method includes the following steps: inoculating the Pantoea agglomerans B50l CGMCC No.1655 into triangle bottle to gain seed liquid, inoculating the seed liquid into fermenting jar special cultivating medium for 48-96 hours. It has good effect to avoid disease, high effect efficiency, high biology safety and simple manufacture technology.

The invention discloses a natural plant extraction composition for cosmetic anti-corrosion. The composition is prepared by compounding 2 to 8 kinds of antibacterial and antifungal plant extracts, and the weight percentage of each kind of extract is 10 to 80 percent. The prepared natural anti-corrosion composition has the advantages that through the matching of plant extracts with antibacterial and antifungal effects, the effects of simultaneously damaging positions of bacterium and fungus cytomembranes, cell walls, nucleic acid, zymoprotein and the like are achieved; the antibacterial capability of the natural plant extraction composition is enhanced, so that the strong-effect and broad-spectrum antibacterial effects can be achieved. The natural anti-corrosion composition is obtained through compounding plant extracts; the antibacterial effect is high; the antibacterial spectrum is broad; the composition can be used as a preservative to be safely and efficiently applied to the fields of cosmetics and the like.

The invention discloses freeze-dried tablets and a preparation method thereof. According to preparation method of the freeze-dried tablets, a functional leavening agent, functional germinated brown rice, Chinese herbal medicine extracts with ambient temperature stress resistance, and a stabilizer capable of effectively improving stability are added in the yoghurt fermentation process; through the adoption of a variable-temperature yoghurt fermentation technology, the propagation of lactic acid bacteria is maximized, and the ambient temperature stress resistance of lactic acid bacteria is improved; yoghurt and fruit puree are pre-processed by adopting a non-thermal processing technology, so that the sundry bacteria contamination is prevented during the process, the juice yield of fruit juice is increased, the content of soluble cellulose of the fruit puree is increased, and the browning of the fruit puree is prevented; in addition, a freezing protecting agent scientifically compounded and having better anti-freezing effect is added during the mixing of the yoghurt and fruit puree; finally, the freeze-dried tablets which are delicate in mouth feel, uniform in texture, stable in state, great in quantity of live bacteria, good in functionality and long in shelf life. The content of live bacteria in the freeze-dried tablets is 1.55*10<11>-2.52*10<11> CFU/g, and the shelf life is 30-36 months at the normal temperature.

The invention discloses cinnamon essential oil loaded Pickering emulsion. The cinnamon essential oil loaded Pickering emulsion is characterized by comprising the following components in a volume ratio: 45-55% of compounded oil phases by using cinnamon essential oil as a main component, 18-23% of a zein solution, 7-12% of a pectin solution and 17-23% of a xanthan gum solution. The cinnamon essential oil loaded Pickering emulsion disclosed by the invention can be diluted with water to have different working concentrations, can still maintain stable and homogeneous state, and can be widely applied to sepsis resistance and fresh keeping of various fruits and vegetables after picking and during the shelf life in the effect manner of coating, soaking and the like.

The invention discloses a chitosan oligosaccharide kojic acid derivative and a preparation method thereof. The chloro kojic acid is mainly reacted with C2-amino(-NH2) of chitosan oligosaccharide to generate a chitosan oligosaccharide derivative, the obtained derivative is analyzed and confirmed by using an infrared spectrum and 1H nuclear magnetic resonance, and the chitosan oligosaccharide molecule is effectively combined with inoculated groups to form the chitosan derivative, wherein the reacted amino groups account for 24.15-55.68% of the total amount of amino in chitosan oligosaccharide. The prepared chitosan oligosaccharide kojic acid derivative is good in solubility and strong in bacteriostatic activity and can be used as a preservative to be widely applied to the fields of medicines and foods.

The invention relates to a Paenibacillus polymyxa Jaas ed2 with the functions of disease prevention and growth promotion to crops and application thereof, and belongs to the technical field of intensive agriculture production. The bacterial strain Jaas ed2 adopted by the invention has been preserved at the General Microbiological Culture Collection Center of China Microbiological Culture Collection Administration Committee on Sep. 18th, 2012 with the preservation number of CGMCC No.6580. The bacterial strain has the antagonistic activity to verticillium lecanii of eggplant, oxysporum of watermelon and other various pathogenic fungi, can enter plants of eggplants and watermelons in case of lower concentration of bacterium for colonization and conduction in the crops, can be used for promoting growth and preventing diseases of crops, and particularly has excellent control efficiency to soil-borne vascular bundle diseases, is low in production cost, simple to operate, facilitates ecological environment protection for farmlands, and reduces pesticide residue when used as a preparation, thereby meeting requirements for production of nuisance free agricultural products in China.

The invention discloses bacillus subtilis JYM841 with antibacterial activity. The bacillus subtilis is preserved in a China center for type culture collection in January 10, 2014, and the preservation number is CCTCC NO: M 2014012. The bacillus subtilis JYM841 has a strong inhibitory effect on staphylococcus aureus and candida albicans, is high in antibacterial activity, wide in antibacterial spectrum, high in enzyme production, sensitive to bacterial antibiotic, drug-resistant to fungal antibiotic, high in heat-resisting ability and tolerant to acid and alkaline, has high antibacterial activity after treated at the temperature of 100 DEG C for 20min, and has tolerance to various enzymes such as trypsin, papain and protease K. An acid and alkali resistant pH (potential of hydrogen) value ranges from 6 to 10, and metabolite is stable, so that the bacillus subtilis can be applied to preparation of medicines for treating genital tract infection.