Bacillus subtilis A16, preparation method thereof and application thereof

A Bacillus subtilis, A16 technology, applied in the field of plant protection, can solve problems affecting human health and the environment, non-target biological toxicity, loss of disease resistance, etc., achieve good development and application prospects, wide antibacterial spectrum, and facilitate industrial production Effect

Inactive Publication Date: 2010-04-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Agricultural prevention and control is mainly through the cultivation of disease-resistant varieties, but some varieties are easy to lose disease resistance, so it is difficult to prevent and control diseases completely through disease-resistant varieties
Although chemical control can effectively control the occurrence and prevalence of diseases, long-term use will cause direct poisoning to non-target organisms, causing environmental pollution and seriously affecting human health and the environment.

Method used

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  • Bacillus subtilis A16, preparation method thereof and application thereof
  • Bacillus subtilis A16, preparation method thereof and application thereof
  • Bacillus subtilis A16, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 Bacillus subtilis A16 is to the plate antagonism of corn leaf spot bacterium and corn leaf spot bacterium

[0030] Bacillus subtilis A16 was obtained by plate face-off screening. In the potato sucrose medium PDA (wherein add 5g / L peptone, the pH value is adjusted to 6.5) plate central insert diameter is 5mm big and small spot mycelia piece of corn, connect three 5mm in diameter symmetrically subsequently The distance between the center of the filter paper and the central mycelium block is 25 mm. Finally, 10 μL of bacterial culture solution was added to each filter paper. The plate not connected with the culture solution of Bacillus subtilis was used as the blank control. The plate was repeated three times and placed at 28 After culturing in an incubator at ℃ for 7 days, the radius of the inhibition zone was measured, which was determined by figure 1 It can be seen that Bacillus subtilis A16 significantly inhibits the growth of corn leaf spot fungus, by f...

Embodiment 2

[0031] Example 2 Indoor Control Efficiency Determination of Bacillus subtilis A16

[0032] Sweet corn seeds sterilized by 0.1% NaClO were treated with culture solution of antagonistic strains (bacteria content was 1.2×10 9 cfu / ml) after soaking for 5 hours, sow in flower pots (soil disinfection), sow 10 seeds in each pot, and set the seeds soaked in sterile water as a control. Sow 10 pots for each treatment, and when the three leaves grow to one new (about 21 days), spray the spore suspension of Pseudomonas spp. and B. maize, place them in the greenhouse for cultivation after inoculation, and investigate the incidence of the disease 7 days after the inoculation of the pathogen. The results showed that the disease indexes of large and small spot diseases of maize plants treated with the A16 strain were 31.4 and 46.3 lower than those of the control, respectively.

Embodiment 3

[0033] Example 3 Antagonism of Bacillus subtilis A16 to various pathogenic bacteria

[0034] The antagonism of Bacillus subtilis A16 against five pathogenic bacteria, Curvularia dahliae, Verticillium dahliae, Wheat scab, Banana anthracnose and Banana fusarium wilt, was tested by plate confrontation method. Screening was carried out with reference to the plate confrontation method. In the center of the potato sucrose medium PDA (adding 5g / L peptone and adjusting the pH value to 6.5) a piece of mycelium of pathogenic bacteria with a diameter of 5mm was placed in the center of the plate, and at a distance of 25mm from both sides of the pathogenic bacteria, an inoculation loop was used to inoculate the endophytic bacteria , with only inoculation of pathogenic bacteria as a control, cultured in an incubator at 28°C for 7 days, measuring the width of the inhibition zone, and repeating 3 times for each treatment. The results are shown in Table 1 and image 3 . From image 3 In Fi...

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Abstract

The invention discloses bacillus subtilis A16, which is preserved in China Center for Type Culture Collection (CCTCC) with CCTCC NO. 209105. The bacterial colony of the Bacillus subtilis A16 is oyster white on a beef extract peptone culture medium, has irregular shape and no luster, is not wet, has irregular edge, has diameter of 0.5-1cm, and has microfold surface, straight rod-shaped thallus and elliptic spore. The invention also discloses a preparation method and application of the bacillus subtilis A16. The bacillus subtilis A16 has broad bacteriostasis, can suppress exserohilum turcicum and helminthosporium maydis, has higher antagonism for exserohilum turcicum bacteria, helminthosporium maydis bacteria, maize curvularia leaf spot fungus, verticillium dahliae kleb, wheat scab pathogen, colletotrichum musae and banana vascular wilt bacteria, and has high prevention cure efficiency, and good safety and development application prospect.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a bacillus subtilis A16 and a preparation method and application thereof. Background technique [0002] Corn big spot is a corn disease caused by the Ascomycete genus Setosphaeria turcica (Luttrell) Leonardet Suggs, which mainly produces large-scale lesion symptoms on the leaves. It is widely distributed. The damage is serious and it is a worldwide disease. The disease occurs and harms to varying degrees in major corn producing areas around the world. Corn small spot is an important leaf disease in warm and humid corn producing areas caused by the Ascomycete Cochliobolus heterostrophus Drechsler, and it is one of the common diseases on corn at home and abroad. It is estimated that the loss of susceptible varieties is about 10% to 20% in the average year of onset, and can reach 30% to 80% in severe cases, or even destroy the species. [0003] At present, the control of large and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/125
Inventor 王振中徐立新董章勇
Owner SOUTH CHINA AGRI UNIV
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