30 results about "Diplodia maydis" patented technology

The invention discloses a pseudomonas aeruginosa D10 which is preserved in China Typical Culture Preservation Center, and the preservation number is CCTCC NO: M 209104. A colony on a beef paste peptone culture medium appears brown, round, glossy and wet, and has trim edges; the diameter of the colony is 0.2-0.5 cm; and the thallus is in a short bar shape and does not have gemma. The invention also discloses a preparation method and applications of the pseudomonas aeruginosa D10. The pseudomonas aeruginosa D10 of the invention can inhibit corn northern and southern leaf blight, has strong antergic functions on corn northern leaf blight, corn southern leaf blight, corn curvalaria leaf spot, cotton verticillium wilt, wheat fusarium graminearum, banana colletotrichum toilette and banana fusarium wilt, has the advantages of wide bacteriostatic range, high prevention efficiency and favorable environmental safety, and has favorable prospects for development and applications.

The invention discloses broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of the broad-spectrum disease resisting, growth promotion and stress resisting bacillus. The broad-spectrum disease resisting, growth promotion and stress resisting bacillus is bacillus sp. WXCDD105, and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation address is 3, No.1 Yard, Western Beichen Road, Chaoyang District, Beijing, the preservation date is May 25, 2016, and the preservation number is CGMCC No.12496. A biocontrol strain provided by the invention has ultraviolet-resisting and drought-resisting properties, has stronger prevention and treatment effects on the tomato gray mold and the tomato leaf mold, and can inhibit fungal diseases including wilt fusarium of a plurality of types of crops, corn foot root bacteria, rice wilt pathogens, acanthopanax senticosus root rot pathogens, corynespora cassiicolai, colletrichum orbiculare, exserohilum turcicum, tomato spot blight fungi, sunflower sclerotinia sclerotiorum, botrytis cinerea, fusarium oxysporum, verticillium fusarium, alternaria alternata and the like; the broad-spectrum disease resisting, growth promotion and stress resisting bacillus has a growth promotion effect on tomato seeds and seedlings and can also improve the quality of fruits and reduce the rotting rate.

The invention discloses an application of alpha-mangostin in controlling plant diseases. Tests prove that alpha-mangostin has agricultural fungicidal activity. The compound has very strong fungistatic action on mango anthracnose pathogens, diplodina sp., botrytis cinerea, and exserohilum turcicum when the testing concentration is 100 mg/L, and has an EC50 value of 18 mg/L-35 mg/L; the compound has certain spore germination inhibition effect on exserohilum turcicum and botrytis cinerea, and the EC50 values are 66.20 mg/L and 50.43 mg/L respectively; meanwhile, in-vivo test results show that the compound has very good protection and treatment effects on rubber tree powdery mildew when the testing concentration is 500 mg/L, and the protection and treatment effects on rubber tree powdery mildew are 75.61% and 70.12% respectively, which are higher than those of a control drug of prochloraz. The above results indicate that alpha-mangostin has broad-spectrum fungistatic action on plant pathogenic fungi, which lays the foundation for the development of novel pollution-free plant-derived pesticides by further using mangosteen shells.

The invention provides a Setosphaeria turcica spore production medium and an application thereof. Every liter of the Setosphaeria turcica spore production medium comprises 20-25g of mannitol, 3-5g of peptone, 40-60g of fresh corn leaves, 30-50g of sorghum grains and 14-16g of agar powder. Setosphaeria turcica is cultured in the spore production medium at 25DEG C in a dark place for 10-15d, aerial mycelia on the surface layer of Setosphaeria turcica are brushed off with sterile water by using a sterilizing writing brush, and light and dark alternate culture is carried out at 25DEG C for 72h to generate a large amount of spores with consistent form and consistent maturity. Corn variety disease resistance is identified through using the Setosphaeria turcica conidiospores generated through culturing by using the medium in order to evaluate the resistance level of corn variety resources to leaf blight, so the prevention and the control of Setosphaeria turcica can be positively realized.

The invention discloses bacillus amyloliquefaciens and application thereof to biocontrol on northern corn leaf blight. 16S rDNA of the bacillus amyloliquefaciens contains a sequence shown as SEQ ID NO: 1. The preferable bacillus amyloliquefaciens is preserved at China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.11488. The provided application means application of the bacillus amyloliquefaciens to inhibit corn leaf blight pathogen. The bacillus amyloliquefaciens provides an easily-colonized novel microbe with strong antagonistic action for biocontrol on corn leaf blight. Through application of bacillus amyloliquefaciens to biocontrol on corn leaf blight, an efficient stable biocontrol method for corn leaf blight is provided, the characteristic is utilized that bacillus amyloliquefaciens can easily colonize at corn leaves and possesses strong antagonistic action on corn leaf blight pathogen, and requirement about control on corn leaf blight in agricultural production is relatively well satisfied.

The invention relates to bacillus amyloliquefaciens and application thereof. The invention aims to solve the problems of the existing chemical agent for controlling leaf blight of corn that the environment is polluted, pathogenic bacteria easily generate drug resistance, and the yield increase effect of corn is not good. The bacillus amyloliquefaciens provided by the invention is bacillus amyloliquefaciens KB1-01 and is collected in China General Microbiological Culture Collection Center (CGMCC), the collection address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the collection date is September 12, 2014, and the collection number is CGMCC No.9649. The application of the bacillus amyloliquefaciens refers to application of the bacillus amyloliquefaciens in inhibition of leaf blight of corn and increase of yield of corn. By using the bacillus amyloliquefaciens, the control effect on the leaf blight of corn is 80.8%, and the yield increase rate of corn is 18.26%. The bacillus amyloliquefaciens is applied to the agricultural field.

The invention provides a corn setosphaeria turcica LAMP detection primer as well as a fast detection method and application thereof, is mainly applied to the specific detection of the corn setosphaeria turcica and belongs to the technical field of crop disease detection and biology. The corn setosphaeria turcica LAMP detection primer is designed, and comprises outer primers F3 and B3, and inner pripers FIP and BIP, shown in a sequence table. According to the corn setosphaeria turcica detection method built on the basis of the primer, the green fluorescence can be observed or trapezoidal stripswith LAMP features can occur through color development reaction after LAMP constant-temperature amplification or agarose gel electrophoresis detection. The LAMP detection primer and the detection method can realize the fast, sensitive and accurate detection of the corn setosphaeria turcica infected plants in the production practice; meanwhile, the primer and the detection method can be used for early diagnosis of the field diseases and the pathogenic bacterium monitoring and identification; the reliable technical and theoretical basis is provided for the early warning and prevention and control of the corn setosphaeria turcica.

The invention discloses a paenibacillus kribbensis metabolite and application thereof in biocontrol. The structural formula of the paenibacillus kribbensis metabolite is shown as a formula (I). The formula (I) is shown in hte description. The active metabolite has high broad-spectrum antifungal activity, particularly remarkable fungistatic activity for plant pathogenic fungi, such as rice sheath blight fungus, corn southern leaf blight fungus and corn northern leaf blight fungus), and can be mass-produced for preventing and controlling plant diseases, and in terms of the prevention and controlof plant fungal diseases, such as rice sheath blight, corn southern leaf blight and corn northern leaf blight and the preparation of corresponding fungus biocontrol agents, the paenibacillus kribbensis metabolite has a good development and application prospect.

The invention relates to the usage of meadow sweet in a bactericide preparation. And it also provides a kind of galenical bactericide which is made from an active component of the fresh or dry meadow sweet extract. Such bactericide can obviously treat of Magnaporthe grisea,Rhizoctonia solani Kuhn,Exserohilum turcicum,fusarium graminearum which are high efficient and low toxin remained during the process.

The invention provides a pseudomonas fluorescens CB113 QS system comQ gene deletion mutant strain and application thereof, and belongs to the technical field of microorganisms. The pseudomonas fluorescens mutant strain M2 is obtained due to the deletion of comQ gene coding sequence 675bp in pseudomonas fluorescens CB113. Compared with the wild type strain, the pseudomonas fluorescens mutant strain M2 has an advantage in application that the inhibitory activity to pathogenic fungus consisting of exserohilum turcicum and cylindrocarpon destructans is obviously improved. The strain provided by the invention shows the characteristics of relatively high fungus inhibiting characteristics and brings a good way for inhibiting fungus for the prior art.

The invention discloses Desertorin B, and an extraction method and application thereof. The molecular formula of the Desertorin B is C25H20O8. The extraction method is as the follows: (1) carrying outstrain cultivation on decaisnea insignis endophytic fungi aspergillus tubingensis DS37, (2) carrying out fermentation cultivation on the strain obtained in the step (1) in a fermentation culture medium, then carrying out extraction with petroleum ether, ethyl acetate and methanol for a plurality of times, mixing the extracting solutions, carrying out filtering, recovering the mixed solvent, carrying out reduced pressure concentration on the filtrate to obtain a crude extract, and finally carrying out silica gel column chromatography to obtain the Desertorin B. The Desertorin B prepared by themethod has a good effect of inhibiting setosphaeria turcica, and can be used for the technologies of agricultural prevention and control and chemical prevention and control. In addition, the Desertorin B has strong activity for resisting MCF-7 and HepG2 cell lines, and can provide a possibility for the development of anti-tumor medicines. At the same time, the Desertorin B has certain antioxidantactivity.

The invention provides a nutritionalhumic acid medical fertilizer and a production method thereof. The production method of the nutritionalhumic acid medical fertilizercomprises the following steps that S01, a solid filter cake is prepared by fermenting leachate from a kitchen; S02, the fixed filter cake is separately identified andcomprises Chinese medicine residue raw materials, vegetable and fruit residues, and edible residual oil derivatives; SO3, the Chinese medicine residue raw materials, the vegetable and fruit residues, and the edible residual oil derivatives are fermented; S04, fermented Chinese medicine residue raw materials, fruits and vegetables residues, and edible residual oil derivative are made into a humic acid medical fertilizer according to a mass percent of apresetproportion. According to the nutritionalhumic acid medical fertilizer and a production method thereof, the preparedhumic acid medical fertilizer has the effects of anti-SOD oxidation, anti-root rot and vine wither, anti pests and diseases such as root-knot nematode, exserohilumturcicum, and fusarium graminerum, and improving soil and stimulating growth.

The invention provides an artificial inoculation method for corn northern leaf blight of greenhouse facilities. The method comprises the three steps of planting management of corns in a greenhouse, inoculation treatment of northern leaf blight of corn plants, and management of corn plants after inoculation, wherein under the greenhouse conditions of facilities, autumn corns with the delayed sowingtime being 20 to 30 days are selected as inoculation objects, at the late whorl stage of corns, exserohilum turcicum conidial suspension obtained after cultivating for 1 to 2 days on a PDA culture medium is adopted for spray inoculation, and the moisturizing conditions of the inoculated plants are optimized, so that on the condition of ensuring the normal growth of corn plants, indoor artificialinoculation of corn northern leaf blight reaches an ideal and stable incidence result. The method solves the problem that in some warm southern areas of China, due to the high temperature in the cornearing stage, the incidence result of artificial inoculation of corn northern leaf blight is poor.

A bacillus amyloliquefaciens preparation and its application. The invention relates to a bacillus amyloliquefaciens preparation and its application. The invention aims to solve the problem that an existing chemical agent for controlling northern leaf blight of corn pollutes the environment, is not safe to human and livestock, makes germs easily generate drug resistance and has unconspicuous corn yield-increasing effects. The bacillus amyloliquefaciens preparation contains, by weight, 0.01-0.02% of zinc sulfate, 0.01-0.02% of boric acid, 0.1-0.2% of Tween 20 and the rest of bacillus amyloliquefaciens KB1-01. The application of the bacillus amyloliquefaciens preparation is an application of the bacillus amyloliquefaciens preparation in inhibiting northern leaf blight of corn and increasing corn yield. Control effect on northern leaf blight of corn is 80.8%, and corn yield-increasing rate is 18.26%. The invention is applied in the field of agriculture.

The invention relates to Alternaria compacta and application thereof, and belongs to the technical field of microorganisms. The Alternaria compacta is preserved in the China General Microbiological Culture Collection Center on July 15, 2021, and the preservation number is CGMCC No. 23046. A liquid culture of the Alternaria compacta has a remarkable inhibiting effect on four fungi including Alternaria maydis, pathogenic bacteria of rhizoma atractylodis wilt, pathogenic bacteria of salvia miltiorrhiza wilt and pathogenic bacteria of salvia miltiorrhiza root rot, and the application prospect is wide.

The invention discloses application of a bactericidal composition containing pyraclostrobin and isoprothiolane and relates to application of the bactericidal composition containing pyraclostrobin andisoprothiolane to reduction or prevention of sigatoka or exserohilum turcicum infecting plants, plant propagating materials and plant organs growing later, soil, material or space and stored matters.The application is characterized in that a ratio of the pyraclostrobin to isoprothiolane ranges from 20:1 to 1:20. In the composition, the pyraclostrobin and isoprothiolane are used according to the ratio capable of achieving a synergistic effect for reducing or preventing infection of the sigatoka or exserohilum turcicum.