30 results about "Diplodia maydis" patented technology

The invention discloses bacillus subtilis A16, which is preserved in China Center for Type Culture Collection (CCTCC) with CCTCC NO. 209105. The bacterial colony of the Bacillus subtilis A16 is oyster white on a beef extract peptone culture medium, has irregular shape and no luster, is not wet, has irregular edge, has diameter of 0.5-1cm, and has microfold surface, straight rod-shaped thallus andelliptic spore. The invention also discloses a preparation method and application of the bacillus subtilis A16. The bacillus subtilis A16 has broad bacteriostasis, can suppress exserohilum turcicum and helminthosporium maydis, has higher antagonism for exserohilum turcicum bacteria, helminthosporium maydis bacteria, maize curvularia leaf spot fungus, verticillium dahliae kleb, wheat scab pathogen, colletotrichum musae and banana vascular wilt bacteria, and has high prevention cure efficiency, and good safety and development application prospect.

The invention discloses a strain of broad-spectrum disease-resistant, growth-promoting, and stress-resistant Pseudomonas nitrogen and its application. The broad-spectrum disease-resistant, growth-promoting, and stress-resistant Pseudomonas nitrogen is Pseudomonas azotoformans) WXCDD51, the date of deposit is July 12, 2016, and the deposit number is CGMCC No.12760. The biocontrol bacterial strain of the present invention has the characteristics of UV resistance and drought resistance, has a strong control effect on tomato gray mold and leaf mold, and can inhibit various crop fusarium wilt, corn stem rot, rice blight, and Acanthopanax Add root rot, cucumber brown spot, cucumber anthracnose, corn leaf spot, tomato blight, sunflower sclerotinia, strawberry gray mold, Fusarium oxysporum, Verticillium, Alternaria and other fungal diseases , and has a growth-promoting effect on tomato seeds, and can also improve fruit quality and reduce rot rate.

The invention relates to the usage of meadow sweet in a bactericide preparation. And it also provides a kind of galenical bactericide which is made from an active component of the fresh or dry meadow sweet extract. Such bactericide can obviously treat of Magnaporthe grisea,Rhizoctonia solani Kuhn,Exserohilum turcicum,fusarium graminearum which are high efficient and low toxin remained during the process.

The invention discloses bacillus amyloliquefaciens and application thereof to biocontrol on northern corn leaf blight. 16S rDNA of the bacillus amyloliquefaciens contains a sequence shown as SEQ ID NO: 1. The preferable bacillus amyloliquefaciens is preserved at China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.11488. The provided application means application of the bacillus amyloliquefaciens to inhibit corn leaf blight pathogen. The bacillus amyloliquefaciens provides an easily-colonized novel microbe with strong antagonistic action for biocontrol on corn leaf blight. Through application of bacillus amyloliquefaciens to biocontrol on corn leaf blight, an efficient stable biocontrol method for corn leaf blight is provided, the characteristic is utilized that bacillus amyloliquefaciens can easily colonize at corn leaves and possesses strong antagonistic action on corn leaf blight pathogen, and requirement about control on corn leaf blight in agricultural production is relatively well satisfied.

The invention discloses a broad-spectrum disease-resistant, growth-promoting, and stress-resistant bacillus for preventing and treating tomato gray mold and leaf mold and its application. The broad-spectrum, disease-resistant, growth-promoting, and stress-resistant bacillus is Bacillus sp. WXCDD105 was deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is May 25, 2016, and the preservation number is CGMCC No. 12496. The biocontrol bacterial strain of the present invention has the characteristics of UV resistance and drought resistance, has a strong control effect on tomato gray mold and leaf mold, and can inhibit various crop fusarium wilt, corn stem rot, rice blight, and Acanthopanax Add root rot, cucumber brown spot, cucumber anthracnose, corn leaf spot, tomato blight, sunflower sclerotinia, strawberry gray mold, Fusarium oxysporum, Verticillium, Alternaria and other fungal diseases , and has a growth-promoting effect on tomato seeds and seedlings, and can also improve fruit quality and reduce rot rate.

The invention discloses an application of alpha-mangostin in controlling plant diseases. Tests prove that alpha-mangostin has agricultural fungicidal activity. The compound has very strong fungistatic action on mango anthracnose pathogens, diplodina sp., botrytis cinerea, and exserohilum turcicum when the testing concentration is 100 mg / L, and has an EC50 value of 18 mg / L-35 mg / L; the compound has certain spore germination inhibition effect on exserohilum turcicum and botrytis cinerea, and the EC50 values are 66.20 mg / L and 50.43 mg / L respectively; meanwhile, in-vivo test results show that the compound has very good protection and treatment effects on rubber tree powdery mildew when the testing concentration is 500 mg / L, and the protection and treatment effects on rubber tree powdery mildew are 75.61% and 70.12% respectively, which are higher than those of a control drug of prochloraz. The above results indicate that alpha-mangostin has broad-spectrum fungistatic action on plant pathogenic fungi, which lays the foundation for the development of novel pollution-free plant-derived pesticides by further using mangosteen shells.

The invention provides a Setosphaeria turcica spore production medium and an application thereof. Every liter of the Setosphaeria turcica spore production medium comprises 20-25g of mannitol, 3-5g of peptone, 40-60g of fresh corn leaves, 30-50g of sorghum grains and 14-16g of agar powder. Setosphaeria turcica is cultured in the spore production medium at 25DEG C in a dark place for 10-15d, aerial mycelia on the surface layer of Setosphaeria turcica are brushed off with sterile water by using a sterilizing writing brush, and light and dark alternate culture is carried out at 25DEG C for 72h to generate a large amount of spores with consistent form and consistent maturity. Corn variety disease resistance is identified through using the Setosphaeria turcica conidiospores generated through culturing by using the medium in order to evaluate the resistance level of corn variety resources to leaf blight, so the prevention and the control of Setosphaeria turcica can be positively realized.