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Spiraeic germicide and its preparing method and use

A fungicide, the technology of Spiraea, which is applied in the field of pesticides, can solve the problems of unseen antibacterial activity of Spiraea, and achieve the effect of low human toxicity

Inactive Publication Date: 2008-03-05
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, no research on the antibacterial activity of Spiraea

Method used

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  • Spiraeic germicide and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation and Antibacterial Activity Determination of Dried Meadowsweet Ethanol Extract

[0023] Steps to prepare dry Spiraea ethanol extract:

[0024] 1. dry Spiraea stems, leaves and flowers are pulverized through a 40-mesh sieve;

[0025] ② Soak in anhydrous ethanol solvent 10 times the weight of the raw material, the extraction temperature is normal temperature, and the extraction time is 24 hours;

[0026] ③ After soaking, stir in a water bath, the temperature is 50°C, and the stirring time is 6 hours;

[0027] ④ obtain the extract after vacuum filtration;

[0028] ⑤ concentrating the extract under reduced pressure with a rotary evaporator, recovering the ethanol to obtain the total ethanol extract of Spiraea chinensis, and concentrating under reduced pressure at a temperature of 45°C;

[0029] ⑥Take a small amount of total ethanol extract and dissolve it with water 50 times the weight of the total extract. The dissolved supernatant is the experiment...

Embodiment 2

[0037] Example 2 Preparation of Fresh Spiraea Leaf and Flower Ethanol Extract and Determination of Antibacterial Activity

[0038] The method for preparing fresh Spiraea ethanol overall and the bioactivity assay method are the same as in Example 1. The experimental results are shown in Table 2.

[0039] Table 2 Inhibition effect of fresh Alpine Spiraea leaves and flowers on pathogenic bacteria

[0040] pathogenic bacteria

[0041] From the results in Table 2, it can be seen that fresh Spiraea leaves and flowers have obvious inhibitory effects on the four pathogenic bacteria, and the effect is better than that of dried Spiraea stems, leaves, and flowers.

Embodiment 3

[0042] Example 3 Preparation and Antibacterial Activity Determination of Dried Meadowsweet Ethyl Acetate and Methanol Extract

[0043] Preparation steps of ethyl acetate extract:

[0044] ① extracting and separating the total ethanol extract of Spiraea chinensis with ethyl acetate to obtain an ethyl acetate extract, further dissolving the solid phase with methanol to obtain a methanol extract, and concentrating to obtain a creamy ethyl acetate and methanol extract;

[0045] ② Dissolve the above-mentioned paste extracts in water with 50 times the weight of the extracts respectively, and the dissolved supernatant is the standby sample for the experiment, and the dissolution temperature is 50°C in a water bath.

[0046] The method for measuring biological activity is the same as that in Example 1. The experimental results are shown in Table 3 and Table 4.

[0047] The inhibitory effect of table 3 ethyl acetate extract on 4 kinds of pathogenic bacteria

[0048] Concent...

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PUM

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Abstract

The invention relates to the usage of meadow sweet in a bactericide preparation. And it also provides a kind of galenical bactericide which is made from an active component of the fresh or dry meadow sweet extract. Such bactericide can obviously treat of Magnaporthe grisea,Rhizoctonia solani Kuhn,Exserohilum turcicum,fusarium graminearum which are high efficient and low toxin remained during the process.

Description

technical field [0001] The invention relates to a botanical fungicide and belongs to the field of pesticides. Background technique [0002] Spiraea, also known as Huoshaojian and Buddha Tea [Miao name: Vobnixngol (Vobnixngol), Mongolian name: Benma] belongs to the genus Spiraea (Spiraea) of the Rosaceae Spiraeae subfamily; it is a plant with light leaves in the Rosaceae family. The whole plant of Spiraea japon ical.f.var.fortunei (Planch) Rehd. There are more than 100 species of this genus in the world, mainly distributed in the northern temperate zone. my country has abundant Spiraea plant resources, and is the modern distribution and differentiation center of this genus, with high species diversity and richness. Distributed in Shaanxi, Shandong, Jiangsu, Anhui, Zhejiang, Jiangxi, Hubei, Sichuan, Guizhou, Yunnan, Inner Mongolia and other provinces and regions, there are more than 70 species. Many species in this genus have beautiful flowers and delicate leaves, and are r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/00A01P3/00A01N65/12
Inventor 侯太平周丽
Owner SICHUAN UNIV
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