Pseudomonas aeruginosa D10, and preparation method and applications thereof

A Pseudomonas aeruginosa, colony technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as affecting human health and the environment, non-target biological poisoning, loss of disease resistance, etc., Achieve good development and application prospects, broad antibacterial spectrum, and easy industrial production.

Inactive Publication Date: 2010-04-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Agricultural prevention and control is mainly through the cultivation of disease-resistant varieties, but some varieties are easy to lose disease resistance, so it is difficult to prevent and control diseases completely through disease-resistant var...

Method used

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  • Pseudomonas aeruginosa D10, and preparation method and applications thereof
  • Pseudomonas aeruginosa D10, and preparation method and applications thereof
  • Pseudomonas aeruginosa D10, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Plate antagonism of Pseudomonas aeruginosa D10 on corn leaf spot fungus and corn leaf spot fungus

[0031] Pseudomonas aeruginosa D10 was obtained by plate face-off screening. In the potato sucrose medium PDA (wherein add 5g / L peptone, the pH value is adjusted to 6.5) plate central insert diameter is 5mm big and small spot mycelia piece of corn, connect three 5mm in diameter symmetrically subsequently The center of the filter paper is 25 mm away from the central hyphae block, and finally 10 μL of bacterial culture solution is added to each filter paper piece, and the plate not connected with Pseudomonas aeruginosa culture solution is used as a blank control, repeated three times, and placed After culturing in an incubator at 28°C for 7 days, the radius of the inhibition zone was measured. figure 1 It can be seen that Pseudomonas aeruginosa D10 significantly inhibits the growth of corn leaf spot fungus, by figure 2 It can be seen that Pseudomonas aeruginosa ...

Embodiment 2

[0032] Example 2 Determination of Pseudomonas aeruginosa D10 indoor control effect

[0033] Sweet corn seeds sterilized by 0.1% NaClO were treated with culture solution of antagonistic strains (bacteria content was 1.2×10 9 cfu / ml) after soaking for 5 hours, sow in flower pots (soil disinfection), sow 10 seeds in each pot, and set the seeds soaked in sterile water as a control. Sow 10 pots for each treatment, and when the three leaves grow to one new (about 21 days), spray the spore suspension of Pseudomonas spp. and B. maize, place them in the greenhouse for cultivation after inoculation, and investigate the incidence of the disease 7 days after the inoculation of the pathogen. The results showed that the disease indexes of large and small spot diseases of maize plants treated with Pseudomonas aeruginosa D10 strain were 35.1 and 57.4 lower than those of the control, respectively.

Embodiment 3

[0034] Example 3 Antagonism of Pseudomonas aeruginosa D10 to various pathogenic bacteria

[0035] The antagonism of Pseudomonas aeruginosa D10 against five pathogenic bacteria, Curvularia dahliae, Verticillium dahliae, Wheat scab, Banana anthracnose and Banana wilt, was tested by plate confrontation method. Screening was carried out with reference to the plate confrontation method. In the center of the potato sucrose medium PDA (adding 5g / L peptone and adjusting the pH value to 6.5) a piece of mycelium of pathogenic bacteria with a diameter of 5mm was placed in the center of the plate, and at a distance of 25mm from both sides of the pathogenic bacteria, an inoculation loop was used to inoculate the endophytic bacteria , with only inoculation of pathogenic bacteria as a control, cultured in an incubator at 28°C for 7 days, measuring the width of the inhibition zone, and repeating 3 times for each treatment. The results are shown in Table 1 and image 3 . From image 3 The ...

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Abstract

The invention discloses a pseudomonas aeruginosa D10 which is preserved in China Typical Culture Preservation Center, and the preservation number is CCTCC NO: M 209104. A colony on a beef paste peptone culture medium appears brown, round, glossy and wet, and has trim edges; the diameter of the colony is 0.2-0.5 cm; and the thallus is in a short bar shape and does not have gemma. The invention also discloses a preparation method and applications of the pseudomonas aeruginosa D10. The pseudomonas aeruginosa D10 of the invention can inhibit corn northern and southern leaf blight, has strong antergic functions on corn northern leaf blight, corn southern leaf blight, corn curvalaria leaf spot, cotton verticillium wilt, wheat fusarium graminearum, banana colletotrichum toilette and banana fusarium wilt, has the advantages of wide bacteriostatic range, high prevention efficiency and favorable environmental safety, and has favorable prospects for development and applications.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to Pseudomonas aeruginosa D10 and its preparation method and application. Background technique [0002] Corn big spot is a corn disease caused by the Ascomycete genus Setosphaeria turcica (Luttrell) Leonardet Suggs, which mainly produces large-scale lesion symptoms on the leaves. It is widely distributed. The damage is serious, and the disease occurs and harms to varying degrees in major corn producing areas around the world. According to reports, in the years and regions where the maize spot disease is severely prevalent, it can lead to a 50% or even more reduction in corn yield. [0003] Corn small spot is an important leaf disease in warm and humid corn production areas caused by the Ascomycete Cochliobolus heterostrophus Drechsler. It mainly damages leaves, and can also damage leaf sheaths, bracts, Ears and grains are one of the common diseases of corn at home and abroad. It i...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/385
Inventor 王振中徐立新董章勇
Owner SOUTH CHINA AGRI UNIV
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