24 results about "Curvularia" patented technology

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis F0b and an application of the bacillus velezensis F0b. The bacillus velezensis F0b is preserved in the Guangdong Microbial Culture Collection Center on April 26, 2024, the preservation date of the bacillus velezensis F0b is April 26, 2024, and the preservation number of the bacillus velezensis F0b is GDMCC (China General Microbiological Culture Collection Center): 64566. The bacillus velezensis has prevention and / or control effects on pyricularia oryzae, cabbage sclerotinia sclerotiorum, tomato fusarium wilt, banana fusarium wilt, rice leaf spot, peronophythora litchii, soybean carbon rot, colletotrichum capsici and corn leaf curvularia, and can be used for preparing broad-spectrum microbial source bactericides. The fermentation liquor of the bacillus velezensis can generate amylase, carboxymethyl cellulose and protease, and the fermentation liquor of the bacillus velezensis has prevention and / or control effects on vegetable pathogenic fungal diseases, namely cabbage sclerotiniose or pepper anthracnose.

The invention discloses a biocontrol complex microbial inoculant for preventing and treating maize curvularia leaf spot and application thereof, and belongs to the field of crop disease prevention and treatment. The biocontrol complex microbial inoculant comprises the following active ingredients: bacillus cereus, pseudomonas chlororaphis and streptomyces microflavus, and / or metabolites thereof. The beneficial microorganisms with different action mechanisms are combined, and the prevention and control effect on the curvularia maize leaf spot disease is enhanced through the synergistic effect. Natural microbial resources are adopted to replace traditional chemical pesticides, harmful substance residues are reduced, and the ecological environment is protected. Through reasonable matching of different types of beneficial microorganisms, effective prevention and control of the curvularia maize leaf spot disease are realized, and healthy growth of plants can be promoted. Compared with a single strain preparation, the complex microbial inoculant provided by the invention has stronger stability and durability, and can significantly reduce the incidence rate of diseases.

The invention relates to a microbial strain and application thereof, in particular to bacillus brevis, a microbial agent prepared from the bacillus brevis and application of the microbial agent. The invention provides the cold-resistant bacillus subtilis which has multiple functions of generating capsular polysaccharide, being high in salt resistance and cold resistance, promoting growth and disease resistance of plants in saline-alkali soil and the like. The bacterial strain can generate capsular polysaccharide, experiments prove that the bacterial strain has high capabilities of dissolving phosphorus, fixing nitrogen and producing siderophore, IAA, ACCD and cytokinin, and has strong antagonistic action on fusarium, curvularia and microplasmodium, and the generated capsular polysaccharide can promote salt tolerance of plants, so that the yield of the plants is increased, and the yield of the plants is increased. The bio-fertilizer is suitable for production of multifunctional bio-fertilizers with disease prevention, growth promotion, salt and alkali resistance, cold resistance and the like, is applied to production of saline-alkali soil grain crops, pasture and greenhouse commercial crops, has remarkable effects of promoting cold damage resistance, root development, prevention and control of root diseases, improvement of crop yield and the like, can reduce the use of chemical fertilizers and pesticides, and has a wide application prospect.

This invention discloses a pesticide composition containing silicon-rich rings and its application in controlling banana leaf spot disease, belonging to the field of biocide pesticide technology. The invention extracts pomegranate seeds. In indoor experiments, the colony diameter of *Curvularia natans* leaf spot pathogen was reduced, indicating that the pomegranate seed extract can inhibit the growth of *Curvularia natans*. Using this extract and silicon-rich rings as synergistic components, it is combined with propiconazole to prepare a pesticide composition containing silicon-rich rings. In field trials, the disease index of the plants decreased, indicating that *Curvularia natans* leaf spot pathogen was inhibited; the complex group showed the highest relative control efficacy, reaching 78.13%. The pesticide composition containing silicon-rich rings prepared by this invention can better inhibit *Curvularia natans* leaf spot pathogen and is suitable for pesticide development for controlling banana leaf spot disease.

The invention discloses an iron-based biological leaf surface protective agent as well as a preparation method and application thereof, and the iron-based biological leaf surface protective agent is prepared from the following raw materials in parts by weight: 2 to 20 parts of EDTA-Fe, 50 to 200 parts of urea, 30 to 100 parts of monopotassium phosphate, 10 to 20 parts of boric acid, 20 to 40 parts of magnesium sulfate, 200 to 400 parts of nano silicon dioxide, 30 to 100 parts of a biological source synergist and 11 to 40 parts of an auxiliary agent, the biological source synergist comprises a Trichoderma asperellum TaLY2 fermentation broth, the Trichoderma asperellum TaLY2 fermentation broth is obtained by inoculating a Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum The iron-based biological leaf surface protective agent disclosed by the invention solves the problems of short effective period, high application frequency and single function of a traditional leaf surface iron fertilizer, has a good prevention and treatment effect on the curvularia leaf spot of corn, further promotes the growth of the corn and improves the yield of the corn.

The invention relates to the technical field of plant disease prevention and treatment, in particular to application of a Welan gum aqueous solution to prevention and treatment of plant diseases, and the concentration of the Welan gum aqueous solution is 50 micrograms per milliliter to 100 micrograms per milliliter. The molecular weight of the welan gum is 800 Da to 900 Da. Experiments prove that the Welan gum aqueous solution can inhibit spore germination and mycelial growth of the original fungus curvularia lunata of the corn curvularia leaf spot, the physiological function of leaves is not affected after spraying, in addition, the Welan gum aqueous solution also has an inhibiting effect on diseases such as northern corn leaf blight and wheat scab, and is expected to be used as a green and safe pesticide, and the Welan gum aqueous solution has broad application prospects. The compound is applied to comprehensive prevention and control of diseases of corn and other crops, and can also be used as a dispersing agent and a slow-release agent to be mixed with other bacteriostatic agents to improve the prevention and control effect.

This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition containing benzimidazole. The pesticide composition's active ingredient is a compound of benzimidazole and fluazinam or emodin methyl ether. This invention, through indoor bioactivity tests, found that the compound of benzimidazole with fluazinam or emodin methyl ether, within a certain mass ratio range, has a synergistic effect against various plant fungal diseases such as Rhizoctonia solani, Curvularia crescentis, and Sclerotium sclerotiorum, helping to improve control efficacy. This is of great significance for the research and development of pesticides for controlling diseases such as cucumber damping-off, corn curvularia leaf spot, and peanut white mold.

The invention relates to a sulfonamide compound containing thiazole heterocycle as well as a preparation method and application of the sulfonamide compound, and belongs to the technical field of agricultural chemicals and bactericides. According to the invention, substituted sulfonyl chloride and unsubstituted or substituted aminoacetophenone are used as main raw materials, the thiazole heterocyclic ring and a sulfonamide group are effectively combined through different reactions to prepare the novel sulfonamide compound containing the thiazole heterocyclic ring, and the structural general formula is shown as a formula I or a formula II. The compound provided by the invention has bacteriostatic activity on multiple plant pathogenic fungi such as sclerotinia sclerotiorum, rhizoctonia solani, botrytis cinerea, curvularia maydis, fusarium oxysporum, pyricularia grisea and the like, and an innovative solution is provided for agricultural sustainable disease prevention and control.

The invention relates to a carbazate compound as well as a preparation method and application thereof. The carbazate compound is a compound shown in a formula (I), or an isotope labeled compound thereof, or an optical isomer, a geometric isomer, a tautomer or an isomer mixture thereof, or a pharmaceutically acceptable salt thereof. The carbazate compound provided by the invention shows broad-spectrum antibacterial activity on plant pathogenic bacteria such as pathogenic fungi; the invention relates to the field of pesticide preparations, in particular to a pesticide preparation for tobacco alternaria alternata pathogenic bacteria, cabbage black spot pathogenic bacteria, tomato early blight pathogenic bacteria, apple anthracnose pathogenic bacteria, corn curvularia pathogenic bacteria, wheat scab pathogenic bacteria, watermelon fusarium wilt pathogenic bacteria, potato dry rot pathogenic bacteria, rice blast pathogenic bacteria, pumpkin fusarium wilt pathogenic bacteria, cotton fusarium wilt pathogenic bacteria, apple ring rot pathogenic bacteria and apple rot pathogenic bacteria. The compound shows excellent bacteriostatic activity on plasmopara viticola, powdery mildew of muskmelon and the like. (I)

The invention relates to the technical field of functional microorganism screening and application, and particularly provides bacillus velezensis and application thereof in prevention and treatment of plant fungal diseases. The bacillus velezensis provided by the invention is a bacillus velezensis separated from rhizosphere of pepper, the Latin name of the bacillus velezensis is bacillus velezensis, the preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) No.29890, and the bacillus velezensis has a strong inhibition effect on fusarium oxysporum CMTT4-1 of pepper fusarium wilt, pathogenic bacteria SYDW1-1 of rice curvularia and colletotrichum gloeosporioides TCNKBLTJ1-1. And on the other hand, the fungicide prepared by utilizing the bacillus velezensis provided by the invention can be used for effectively preventing and treating pepper fusarium wilt and promoting pepper growth when being applied to peppers, and compared with traditional chemical pesticides and chemical fertilizers, the fungicide for biological prevention and treatment has the characteristics of being healthy to human and livestock, low in cost, environment-friendly and the like, conforms to the sustainable development principle and has a wide application prospect.

The invention discloses a microbial agent for preventing and treating tomato root rot, and belongs to the technical field of microbial materials. According to the invention, three biocontrol strains, namely bacillus licheniformis, bacillus subtilis and monascus curvularia, are obtained through screening, and the three biocontrol strains have an inhibition effect on a plurality of pathogenic bacteria, and particularly have a relatively strong inhibition effect on fusarium oxysporum. A small amount of glucose, soybean cake powder and the like are added to form a composite nutrient solution, and sufficient nutrition is provided for microbial activities. A microbial activity accelerant composed of alginate oligosaccharide and 5-aminolevulinic acid is added, so that the microbial colonization efficiency is improved. In actual tomato planting, the tomato root rot can be effectively prevented and treated, meanwhile, tomato plant growth is promoted, tomato quality is improved, the method is green, safe and pollution-free, environmental pollution is avoided, the dosage of other corresponding chemical pesticides can be reduced, expenditure is saved, cost is reduced, potential economic benefits and environmental benefits are achieved, and the method is worthy of application and popularization.

The invention discloses Streptomyces sp.Zm3-3, a microbial agent and application of the microbial agent, and belongs to the technical field of microorganisms. According to the invention, a strain of Streptomyces sp.Zm3-3 is separated and screened from corn leaves, and the strain can antagonize the growth of hyphae of Zea maydis and curvularia lunata. Indoor potting and field control effect determination results show that the control effect of the Zm3-3 fungicide on corn southern leaf blight and corn curvularia leaf spot exceeds 70%, and the control effect on corn rust exceeds 60%. The Zm3-3 microbial inoculum and the fermentation filtrate thereof can digest hyphae of Zea maydis and curvularia lunata and inhibit germination of conidia of pathogenic bacteria. The biocontrol microbial inoculum prepared from the strain can replace or reduce the use of chemical pesticides, accords with the new idea of green plant protection development, is beneficial to guarantee of food and ecological safety, and has good economic and social benefits.

The present invention discloses a Panax notoginseng sterol C-22 desaturase gene PnCYP710A and its applications, PnCYP710A The nucleotide sequence of the gene is shown in SEQ ID NO: 1, encoding a sterol C-22 desaturase of the plant cytochrome P450 monooxygenase family. The invention has been confirmed by functional genomics related technology research. PnCYP710A The gene has the function of improving the resistance of plants to fungal infection. PnCYP710A The gene was constructed into a plant expression vector and transferred into tobacco for expression. PnCYP710A The resistance of transgenic tobacco to Fusarium solani and Curvularia solani was significantly improved.

The invention belongs to the technical field of microorganisms, and particularly relates to saccharomyces boulardii BHS09, a microbial agent and application of the saccharomyces boulardii BHS09 in agriculture. The preservation number of the bacterial strain BHS09 provided by the invention is GDMCC (Graphite December for March Center) No. 66542. The strain has high phosphorus dissolving capacity and potassium dissolving capacity, and the potassium dissolving rate is 46.83%; the capacity of high-yield IAA is achieved, and the yield reaches 24 mg / L; the inhibition rates of the volatile gas to curvularia maize, fusarium moniliforme, fusarium pseudograminearum and fusarium oxysporum are respectively 11.64%, 54.82%, 46.48% and 51.80%, and the inhibition rates of the volatile gas to rhizoctonia solani, fusarium verticillium, curvularia maize, fusarium moniliforme, fusarium pseudograminearum, fusarium graminearum, phytophthora nicotianae and fusarium oxysporum are 40.05%-76.02%; the strain has an obvious growth promoting effect on wheat, and provides a strain resource for excavation of composite microbial strains with multiple functions.

The invention provides bacillus vallismortis TCS004 with a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.25850. The invention discloses an application of the bacillus vallismortis TCS004 in preventing and treating plant pathogenic fungi or plant pathogenic bacteria, or an application of the bacillus vallismortis TCS004 in preparing a microbial preparation for preventing and treating the plant pathogenic fungi or the plant pathogenic bacteria. The bacterial strain has a very high inhibition effect on plant pathogenic fungi such as lasiodiplodia mali, macrostem botryosphaeria verticillata, citrus interspersonia, streptosclerotinia sclerotiorum, alternaria alternata, colletotrichum frutescens, muscardine fungus, alternaria solani, fusarium solani, botrytis cinerea, fusarium equisetum, curvularia, alternaria alternata and the like; and the compound also has relatively good bacteriostatic activity on plant pathogenic bacteria xanthomonas oryzae and the like. The invention further provides a formula of a fermentation culture medium of the bacillus vallismortis TCS004 and fermentation process conditions of the bacillus vallismortis TCS004.

The invention discloses a strain of extreme oriental pseudomonas LJ-6 and application thereof in prevention and control of mulberry diseases. The extreme oriental pseudomonas disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on June 18, 2024, and the preservation number of the extreme oriental pseudomonas is GDMCC (China General Microbiological Culture Collection Center) NO: 64766. The extreme pseudomonas orientalis LJ-6 has a very good antagonistic effect on bacterial pathogenic bacteria such as lauraceae bacteria, pantoea anantoea, Klebsiella compacta and enterobacter cloacae and fungal pathogenic bacteria such as fusarium oxysporum, curvularia and colletotrichum truncatum, and has a very good antagonistic effect on plant diseases caused by plant infection by the pathogenic bacteria. The composition has good prevention and control effects on mulberry bacterial wilt, mulberry bacterial wilt, mulberry leaf spot, mulberry anthracnose and the like, and is safe and effective. Therefore, the extreme oriental pseudomonas LJ-6 disclosed by the invention has good application prospect and value in the aspects of preventing and controlling plant diseases such as mulberries and preparing disease prevention and control preparation products.

The invention belongs to the technical field of molecular biological detection, and particularly relates to a primer pair, a detection reagent and an RPA-LFD kit for visually, ultrasensitively and rapidly detecting puccinia zeae and application. The sequences of an upstream primer and a downstream primer of the RPA primer pair are respectively shown as SEQ ID NO: 1 and SEQ ID NO: 2. The RPA primer pair can be used for visually detecting the puccinia zeae through RPA amplification or RPA-LFD by naked eyes, the sensitivity is ultrahigh (0.01 pg / mu L), and the specificity is strong; and no specific band exists on puccinia polystachys, bipolaris maydis, corn ear rot pathogen and curvularia maydis leaf spot pathogen.

The invention belongs to the field of chemical synthesis, and particularly relates to a 4-aryl naphthoimidazole compound as well as a preparation method and application thereof. The chemical structural formula of the 4-aryl naphthoimidazole compound is as shown in formula I. The 4-aryl naphthoimidazole compound is easy to synthesize and has extremely high inhibitory activity on fungi such as apple anthracnose pathogenic bacteria, apple ring rot pathogenic bacteria, rice blast pathogenic bacteria, cabbage black spot pathogenic bacteria and / or corn curvularia pathogenic bacteria; the effect is obviously better than that of the currently common clinically applied antifungal drug azoxystrobin. The preparation method disclosed by the invention has the advantages of short synthesis route, simple and easily available raw materials, simple post-treatment and the like, and is suitable for industrial large-scale production. # imgabs0 #

The invention discloses multifunctional cold-resistant bacillus subtilis and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of the Peribacillusfrigitorirans YM763 is obtained through separation and screening, and the strain can be used for preventing and treating corn diseases caused by Curcularia lunata, Exserohilum tubers and Bipolar maydis at the same time and degrading a chemical insecticide chlorantraniliprole, and has no cross resistance with existing bactericides and insecticides. The invention further provides a preparation method of the strain of the Peribacillusfrigitorirans YM763, and the strain of the invention has the advantages that the strain of the Peribacillusfrigitorirans YM763 can be used for preventing and treating corn diseases caused by Curcularia lunata, Exserohilum tubers and Bipolar maydis and degrading the chemical insecticide chlorantraniliprole; compared with the existing chemical synthesis pesticide for prevention and treatment, the method has the advantages of high efficiency, low toxicity, no environmental pollution and low possibility of generating drug resistance because of being from the natural environment.

The invention belongs to the technical field of pesticides, and particularly relates to a composition containing a plant immune resistance inducer and a chemical bactericide and application of the composition to promotion of disease resistance and yield increase of corn. The disease index of corn southern leaf blight and corn curvularia leaf spot disease treated by the composition is low and is superior to or close to the prevention effect of a conventional chemical agent treatment group (100% color development), and the yield of corn treated by the composition per mu is increased by 9.7% or above and is close to the yield increase value of the conventional chemical agent treatment group (100% color development).

The invention belongs to the technical field of microorganisms and genetic engineering, and particularly relates to a chitinase mutant and application thereof in promoting plant growth. According to the present invention, by using the error-prone PCR technology, the direct evolution is performed on the wild type chitinase in vitro so as to obtain the chitinase mutant with significantly improved enzyme activity and significantly improved environmental stability; the enzyme activity of the mutant is 1051.36 U / mL under the conditions that the temperature is 50 DEG C and the pH value is 7 and is about 2.7 times that of wild chitinase (385.45 U / mL), and the mutant not only has a good killing effect on pests such as lepidoptera pests, thysanoptera pests and coleoptera pests, but also has good bacteriostatic activity on pathogenic bacteria such as phytophthora capsici, pythium aphanidermatum, botrytis cinerea, potato early blight and corn curvularia, and has good application prospects. The fertilizer is used for growth and development of vegetable plants or grain plants, and can promote plant growth and significantly improve the yield and quality of plants.

The invention belongs to the technical field of microbial agents, and discloses acremonium spp. NtSi1 and application thereof in preparation of a root rot prevention and control agent. The acremonium spp. NtSi1 bacterial liquid provided by the invention has a wide inhibition effect on plant pathogenic bacteria, and can be used for preventing and treating plant root rot caused by Fusarium oxysporum, Fusarium solani, Fusarium falciforme, Alternaria sp, Curvularia spp and the like, and the acremonium spp. NtSi1 bacterial liquid can be used for preventing and treating the plant root rot caused by the Fusarium oxysporum, the Fusarium solani, the Fusarium falciforme, the Alternaria sp. Or the Curvularia spp. Tests prove that the control effect of the biocontrol microbial inoculum on mung bean root rot under the potting condition reaches 66.21%, and the control effect of the biocontrol microbial inoculum on tobacco root rot reaches 63.46%.

The present application relates to the field of microorganism, specifically relates to the application of Tolypocladium inflatum O1-SF-04630 and its prevention and treatment of plutella xylostella, the preservation number of the Tolypocladium inflatum O1-SF-04630 strain (Tolypocladium inflatum O1-SF-04630) is CCTCC NO:M 20241094, the research has confirmed that the strain has strong pathogenicity to plutella xylostella, has the potential to be developed as biological control agent, and can be applied to the prevention and treatment of plutella xylostella pest.

The invention relates to a substituted tetrahydro-beta-carboline derivative as well as a synthesis method and application thereof. The preparation method comprises the following steps: by taking different carboxylic acids as raw materials, adding a solvent anhydrous dichloromethane for dissolving, reacting with thionyl chloride under the condition of oil bath heating to prepare a corresponding acyl chloride substrate, then carrying out amidation reaction with a tetrahydro-beta-carboline compound, adding a quenching agent for quenching reaction, and finally obtaining the tetrahydro-beta-carboline compound. And carrying out reduced pressure concentration to remove dichloromethane to obtain the substituted tetrahydro-beta-carboline derivative. The compound has the application of inhibiting phytopathogen. The phytopathogen is pythium aphanidermatum, cotton verticillium wilt, alternaria alternata, botrytis cinerea, curvularia maize, eggplant verticillium wilt, fusarium oxysporum, cucumber target leaf spot, tomato alternaria solani, watermelon fusarium wilt, cucumber brown spot, apple alternaria leaf spot and sclerotinia sclerotiorum. The synthesis process is simple, and the product purity is high.