44 results about "Plasmopara viticola" patented technology

The present invention relates to compositions containing the fungus Alternaria alternata and, or the secondary metabolites thereof, belonging to the family of the diketopiperazines (DKPs), for controlling the pathogen fungus Plasmopara viticola.

The invention belongs to the technical field of crop disease diagnosis and control, and in particular relates to a molecular detection method and application of plasmopara viticola. According to the molecular detection method, a group of looped-mediated isothermal amplification (LAMP) primers are self-designed through bioinformatic analysis according to the internal transcribed spacer (ITS) sequence information of a variety of disclosed peronosporales fungi and other common plant pathogenic fungi; and the existence of the deoxyribonucleic acid (DNA) of a plasmopara viticola genome at the level of 33fg / mu l can be rapidly detected from the plasmopara viticola samples of different varieties in different grape growing regions within only 55 minutes by combining a rapid extraction technology for the genome of the plasmopara viticola and adopting a one-step LAMP reaction. The rapid molecular detection method has high specificity, sensitivity and detection accuracy, and wide application range, and can be popularized and applied in various places as a rapid detection technology for the plasmopara viticola.

The invention discloses a compound composition containing 2-(p-fluorophenyl)-5-methanesulfonyl-1,3,4-oxadiazole and Copper(succinate+glutarate+adipate) and a preparation. The compound composition is composed of 2-(p-fluorophenyl)-5-methanesulfonyl-1,3,4-oxadiazole and Copper(succinate+glutarate+adipate), wherein the weight ratio of 2-(p-fluorophenyl)-5-methanesulfonyl-1,3,4-oxadiazole to Copper(succinate+glutarate+adipate) is (20-1):(1-40). The compound composition disclosed by the invention accounts for 0.1-90% of the weight percentage of a bactericide and can be used for effectively preventing and controlling various bacterial diseases such as citrus canker and cucumber bacterial angular leaf spot disease and fungous diseases such as plasmopara viticola. The compound composition has a remarkable synergistic effect and is high in preventing effect and capable of reducing the dosage of a pesticide as well as preventing and treating costs and also delaying the pesticide resistance.

The invention discloses a biocontrol strain and agent capable of preventing and controlling plasmopara viticola and phytophthora capsici and an application of the biocontrol strain and agent. The biocontrol strain is characterized by being a streptomyces atratus py-1 strain in surface soil of a grapery and is collected with the number of China General Microbiological Culture Collection Center (CGMCC) No:7826 in CGMCC; and a secondary metabolite of streptomyces atratus py-1 is used as an active component of the biocontrol strain. The biocontrol agent is prepared through the steps of inoculating the streptomyces atratus py-1 into 200ml of psa culture solution in a 500ml triangular flask to culture for 5 days; centrifuging a fermentation solution for 30 minutes; filtering a supernatant solution by using a filter membrane to obtain a stock solution; diluting the stock solution, then, spraying the diluted stock solution on grapes, and irrigating the diluted stock solution into the roots of hot peppers. The biocontrol agent has a certain effect on inhibiting egg plant stem blight, egg plant root rot, tomato spotted wilt virus, tomato late blight, cucumber anthracnose, maize ear rot, sorghum curvularia lunata and wheat root rot.

The invention discloses a bactericidal composition containing fluorobactaramide and metrafenone and an application thereof. The bactericidal composition uses fluflubactam and metrafenone as main active ingredients, wherein the mass ratio of fluflubactam and metrafenone is 1-70:1-50. The bactericidal composition can be applied to the prevention and treatment of cereals, fruit trees, and vegetable diseases, has a high synergistic effect, overcomes and delays the drug resistance of pathogens, has fast sterilization speed, long-lasting effect, reduces application costs, and has good control effect. Significantly better than its single dose use. The present invention can be used to prevent and treat fungal diseases on crops, especially can be used to prevent and control fungal diseases such as grape downy mildew, capsicum phytophthora, potato late blight, rice sheath blight, cotton blight, wheat powdery mildew, etc., and the effect is remarkable and excellent. For single dose use.

The invention relates to a bactericidal composition with a synergistic effect, which has an effective active component containing mandipropamid and cyazofamid, wherein the weight ratio of the mandipropamid to the cyazofamid is (1-85) : (85-1). The effective active component is added with auxiliaries and polybutene so as to be prepared into wettable powder, water dispersible granules and suspending agent, and has very high biological activity for oomycetes fungus such as phytophthora, plasmopara viticola, pseudoperonospora cubensis, pythiumuhtimum, and plasmodiophora brassicae woron of plasmodiophoromycetes.

The invention provides a method for the indoor culture breeding of Plasmopara viticola. The method comprises the following steps: 1, acquiring a Plasmopara viticola sample; 2, preparing a sporangium suspension; 3, selecting the grape variety and blade; 4, inoculating the sporangium suspension; and 5, carrying out blade moisturizing culture. The method has the advantages of simplicity, easy learning, low requirements on a tester, and culturing for 7-10 days under a same environment condition after blade morbidity, overcomes the defects comprising difficult survival, no indoor mass propagation of the Plasmopara viticola sample, and sporangium suspension insufficiency after the previous inoculation of the Plasmopara viticola, can be used for monitoring the Plasmopara viticola and determining the pathogenicity, and has very high practical application values.

The invention relates to an antagonistic strain which is finally identified as Bacillus amyloliquefaciens through physiological and biochemical reaction, 16SrDNA and gyrA gene sequencing and sequence alignment; the Bacillus amyloliquefaciens is named Bacillus amyloliquefaciens YTB1407. The Bacillus amyloliquefaciens strain has antagonistic activity to various fruit tree pathogenic fungi, including macrophoma kawatsukai, ahernaria mali, grape anthracnose, coniothyrium diplodiella, plasmopara viticola and the like; the Bacillus amyloliquefaciens strain is antibacterial in broad spectrum, stable in anti-microbial activity and lasting in effect and has the huge potential for developing novel, efficient and natural biological control agents.

The present invention provides an agricultural fungicide, which uses Sclerotiorin or a salt thereof as an effective component, and is used for preventing and controlling agricultural diseases caused by Sclerotinia sclerotiorum, Monographella nivalis, Gaeumannomyces graminis, Septoria tritici, Glomerella lagenarium, Mycosphaerella arachidis and Plasmopara viticola.

The invention relates to a sterilizing composition containing pyraclostrobin and quinolinone, wherein the mass part ratio of the pyraclostrobin to the quinolinone is (1-20):(30-1). The sterilizing composition is suitable for preventing and treating plasmopara viticola. The pyraclostrobin and the quinolinone are mixed to achieve an obvious synergistic effect, the use amount of pesticide is reduced,and the sterilizing composition has large economic benefit and social benefit when being popularized and applied.

The invention relates to a method for extraction of an active metabolite from a Streptomyces corchorusii NF0919 strain (with a preservation number of CGMCC No.3968 in CGMCC and a strain patent number of ZL201010236886.1) fermentation broth. The metabolite can effectively control Grape Anthracnose caused by plant pathogenic fungus Glomerella cingulata infection, Caucasus grape black rot caused by Physalospora baccae infection, Grape Alternaria Rot caused by Alternaria viticola infection, Grape white rot caused by Coniothyrium dlodiella infection, Grape Downy mildew caused by Plasmopara viticola infection, grape grey mould caused by Botrytis cinerea infection, grape spot anthracnose caused by Elsinae ampelina infection, and other plant diseases.

The invention discloses a sterilization composition containing diacetone amine copper oxalate. The sterilization composition containing the diacetone amine copper oxalate comprises a first active component and a second active component with the weight ratio of (1 to 10):(10 to1), wherein the first active component is the diacetone amine copper oxalate; the second active component is selected from one of dimethomorph, flumorph, metalaxyl, metalaxyl-M, fosetyl-Al, benalaxyl, oxadixyl, cymoxanil, propamocarb, cyazofamid and iprovalicarb. The composition can be prepared into a water dispersible granule, wettable powder or a dispersible oil suspending agent which are allowed to be used agriculturally, and has good control effects to cucumber downy mildew, potato late blight, tomato early blight and plasmopara viticola. According to the composite, the problem of non-ideal control effect by only utilizing diacetone amine copper oxalate or oomycetes fungicides is solved effectively; the compounding of diacetone amine copper oxalate and oomycetes fungicides has the obvious synergism, has the good crop safety, and can be used for reducing the risk of resistance production and drug delivery cost.

The invention discloses a management method for preventing and treating grape downy mildew. According to the management method, novel microorganism organic fertilizers are applied, plasmopara viticola can be killed by virtue of antagonism of microorganisms, meanwhile multiple assistant measures are taken to treat both symptoms and root causes, and thus fruit trees suffering from diseases can be thoroughly cured. The management method disclosed by the invention is capable of reducing the amounts of chemical fertilizers and pesticides, plays an active role in protecting and utilizing soil and ambient agricultural environments, is capable of reducing the planting cost and increasing the economic benefits and the social benefits, and has wide application prospects.

The invention belongs to the field of biotechnology equipment, and in particular relates to a petri dish for cultivating plasmopara viticola. The petri dish comprises a petri dish cover (1), a petri dish box (3) and a support sieve (2), wherein the petri dish cover (1) is provided with a cover edge (1.1), a cover ring (1.2) and a hemispherical mist flushing point (1.3); the support sieve (2) is provided with a sealing ring (2.1), sieve pores (2.2) and support pillars (2.3); the petri dish box (3) is provided with a mist inlet tube and a water outlet tube, and a mist outlet (3.3) of the mist inlet tube is formed upwards in an inclined way; the water outlet tube is internally provided with through degreasing cotton (4.3). A plate is made from a transparent plastic material, so that the growth situation of the plasmopara viticola in the petri dish can be conveniently observed. The petri dish reduces the probability that leaves are infected by bacteria; the petri dish is internally connected with a humidifier, so that the mist is even during culturing, and the cultured plasmopara viticola is higher in activity.

The invention discloses a plant immune activator protein secreted by peronospora viticola, a primer and application. The amino acid sequence of the plant immune activator protein is as shown in SEQ ID NO.2, the nucleotide sequence of the coding gene of the plant immune activator protein is as shown in SEQ ID NO.1, the amino acid sequence of a mutant PvAvh77-M2 of the plant immune activator protein is as shown in SEQ ID NO.4, and the nucleotide sequence of the coding gene of the plant immune activator protein is as shown in SEQ ID NO.3. The mutant PvAvh77-M2 of the plant immune activator protein can induce expression of grape disease-resistant related genes and accumulation of defensive substances, inhibit infection of plasmopara viticola and reduce harm of downy mildew to grapes, and can be used as a biopesticide for preventing downy mildew of the grapes.

The invention provides a method for the indoor culture breeding of Plasmopara viticola. The method comprises the following steps: 1, acquiring a Plasmopara viticola sample; 2, preparing a sporangium suspension; 3, selecting the grape variety and blade; 4, inoculating the sporangium suspension; and 5, carrying out blade moisturizing culture. The method has the advantages of simplicity, easy learning, low requirements on a tester, and culturing for 7-10 days under a same environment condition after blade morbidity, overcomes the defects comprising difficult survival, no indoor mass propagation of the Plasmopara viticola sample, and sporangium suspension insufficiency after the previous inoculation of the Plasmopara viticola, can be used for monitoring the Plasmopara viticola and determining the pathogenicity, and has very high practical application values.

The invention discloses a composition containing oxathiapiprolin and copper abietate. The proportion of the oxathiapiprolin to the copper abietate is 1:30-30:1, and preferably 1:10-20:1. The composition has good synergistic effect for plasmopara viticola in the matching range.

The invention relates to a bactericide composition containing an artemisia capillaris thunb extractive and propamocarb hydrochloride as well as application of the bactericide composition. The bactericide composition comprises active ingredients of the artemisia capillaris thunb extractive and propamocarb hydrochloride, wherein the weight ratio of the artemisia capillaris thunb extractive and propamocarb hydrochloride is 30:1 to 1:30. The invention further relates to a bactericide comprising the bactericide composition and pesticide acceptable carriers and additives. Furthermore, the invention further relates to application of the bactericide composition or the bactericide to prevent and control phytophthoya capsici leonian, phytophthora melonis or plasmopara viticola. Compared with the single dosage, the synergic coefficients of the artemisia capillaris thunb extractive and propamocarb hydrochloride after composition are both 130 above, so that the bactericide composition is good in synergic function; meanwhile, the bactericide composition can suspend the drug resistance of the single dosage well.

The invention discloses a bactericidal composition containing azoxystrobin and fenaminstrobin and application thereof. The bactericidal composition uses the azoxystrobin and the fenaminstrobin as main effective ingredients, wherein the mass ratio of the azoxystrobin to the fenaminstrobin is (70:1) to (1:50). The bactericidal composition can be applied to prevention and control of cereal, fruit tree and vegetable diseases, has a higher synergistic interaction effect, overcomes and delays the drug resistance of pathogenic bacteria and is high in sterilization speed and long in lasting period, the application cost is reduced, and a prevention and control effect is obviously superior to the effects of single chemical pesticides in use. The bactericidal composition can be used for preventing and controlling fungous diseases of crops, especially can be used for preventing and controlling powdery mildew, wheat glume blotch, wheat rust, wheat powdery mildew, rice sheath blight disease, powdery mildew of cucumbers, downy mildew of cucumbers, plasmopara viticola, apple powdery mildew, tomato early blight, pear scab and other fungal diseases and is better than the single chemical pesticides in effect in use.

The invention discloses a method rapidly identifying a cucumber breeding material with heat resistance and moisture resistance without damaging a seedling stage, which comprises the following steps carried out in a manual simulation environment with daytime / night=12hr / 12hr, daytime temperature of 35 DEG C, night temperature of 25 DEG C, daytime humidity of 80%, night humidity of 100% and daytime illumination intensity of 40,000lux: (1) carrying out first screening on the seventh day after cucumber sowing, and selecting seedlings with the length of hypocotyls not exceeding 4cm; (2) on the ninth day after sowing, selecting the seedlings where first main leaves have the sizes not exceeding 4*5cm and the main leaves and edges of cotyledons are free of thermal burn; and (3) respectively inoculating plasmopara viticola and zucchini yellow mosaic virus (ZYMV) on the seedlings selected in the step (2) at the period of a third main leaf. The seedlings having heat resistance and moisture resistance to the plasmopara viticola and the ZYMV are the cucumber breeding material with the heat resistance and the moisture resistance. The method rapidly identifying the cucumber breeding material with the heat resistance and the moisture resistance without damaging the seedling stage causes no damage to the seedlings, can identify the cucumber breeding material with the heat resistance and the moisture resistance through three simple morphological characteristic indexes and is learned and operated easily. Besides, the method is good in relevance and high in accuracy rate.

The invention provides a method for detecting overwintering amount of plasmopara viticola in soil by real-time fluorescence quantitative PCR. The method of the invention is of great significance for accurately estimating the field disease condition in an early stage, determining the disease place, and formulating timely and effective control measures; a specific primer pair Pv5 / Pv6 is designed according to a full-length ITS gene (2337 bp) of plasmopara viticola, and a molecular detection system of plasmopara viticola real-time PCR is constructed, to quantitatively detect the overwintering amount of plasmopara viticola in soil, and to provide technical support for early monitoring, early warning and accurate prevention and control of plasmopara viticola in the field.

The invention discloses an in vitro preservation method of plasmopara viticola. According to the method, plasmopara viticola is embedded in agar powder, and then preserved at -80 DEG C; after 90 daysof preservation, healthy leaves of grapes are still pathogenic for back grafting; and a specific preservation treatment method and conditions of plasmopara viticola, and the processes of determinationof sporangium pathogenicity after preservation are also included in the method. The method provided by the invention is an ideal, simple and effective preservation method.

The invention provides a bactericidal composition, the bactericidal composition contains Pseudomonas fluorescens and Gliocladium pink chlamydospores, the mass ratio of Pseudomonas fluorescens and Glioma pink chlamydospores is 1000 :1~1:1000. The fungicidal composition provided by the present invention shows obvious synergistic effect in preventing and treating downy mildew diseases caused by Plasmodium by combining Pseudomonas fluorescens and chlamydospores of Gliocladium rosea, and reduces the existing plant pathogenic bacteria The risk of potential resistance to chemical agents; the combination of Pseudomonas fluorescens and chlamydospores of Gliocladium pinkum has obvious synergistic effect, and at the same time reduces the dosage of agents and is more environmentally friendly, so that the composition can be widely used in the prevention and treatment of Downy mildew disease caused by Plasmodium unipara.

The invention discloses a sterilization composition containing fluoride ether bacteria amide and propiconazole and application thereof. Fluoride ether bacteria amide and propiconazole are taken as main effective ingredients of the sterilization composition, wherein the mass ratio of fluoride ether bacteria amide to propiconazole is 1-70:1-50. The sterilization composition can be applied to prevention of cereal, fruit tree and vegetable diseases, have high synergistic effects and overcome and delay pesticide resistance of harmful bacteria, and is quick in sterilization speed and long in lasting period; the application cost is lowered, and the prevention effect is obviously better than that of a single pesticide of the sterilization composition. The sterilization composition can be used for prevention of fungi on crops and can particularly be used for prevention of fungal diseases such as plasmopara viticola, potato late blight, rice sheath blight, cotton seedling damping-off, wheat take-all, powdery mildew, rust disease, root rot, rice bakanae disease and sigatoka, and the effect of the sterilization composition is remarkably better than that of the single pesticide.

The invention relates to an antagonistic strain which is finally identified as Bacillus amyloliquefaciens through physiological and biochemical reaction, 16SrDNA and gyrA gene sequencing and sequence alignment; the Bacillus amyloliquefaciens is named Bacillus amyloliquefaciens YTB1407. The Bacillus amyloliquefaciens strain has antagonistic activity to various fruit tree pathogenic fungi, including macrophoma kawatsukai, ahernaria mali, grape anthracnose, coniothyrium diplodiella, plasmopara viticola and the like; the Bacillus amyloliquefaciens strain is antibacterial in broad spectrum, stable in anti-microbial activity and lasting in effect and has the huge potential for developing novel, efficient and natural biological control agents.

The invention discloses a metalaxyl-M and propamocarb hydrochloride compounded aqueous solution and an application thereof. The metalaxyl-M and propamocarb hydrochloride compounded aqueous solution is prepared from, by weight, 1-20% of metalaxyl-M, 1-60% of propamocarb hydrochloride, 3-30% of thymol, 5-20% of a surface active agent, 0.1-2% of stabilizer, 0.1-0.3% of defoamer and the balance of water. The metalaxyl-M and propamocarb hydrochloride compounded aqueous solution is efficient, safe and environment friendly, synergistic effect on the component is achieved, crop output can be increased, quality of fruit is improved, cost is lowered and generation of drug resistance to bacteria is beneficially suspended; the metalaxyl-M and propamocarb hydrochloride compounded aqueous solution can be used for preventing and controlling crop diseases of citrus burning disease, corynespora cassiicola, pepper phytophthora blight, rice seedling blight and rice blast, plasmopara viticola and the like.

The invention discloses a compounded composition containing isolongifolenoneoximelactam and flutriafol as well as a preparation containing the isolongifolenoneoximelactam and the flutriafol. The compounded composition is composed of the isolongifolenoneoximelactam and the flutriafol, wherein the weight ratio of the isolongifolenoneoximelactam to the flutriafol is (20-1):(1-40); and the compoundedcomposition accounts for 0.1%-90% of the weight of the bactericide. The compounded composition provided by the invention can effectively prevent and control various bacterial diseases such as citrus canker diseases and cucumber bacterial angular leaf spot diseases, and can also prevent and control fungal diseases such as grape plasmopara viticola; and the compounded composition has obvious synergistic effects, the prevention effects are high, the use amount of pesticides is reduced, the prevent and control costs are reduced, and the drug resistance is delayed.

The invention discloses a bactericidal composition containing dikestrobin and acetostrobin. The film component is 0.5%-15%, and the rest are additives and water, and the percentage concentration is weight percentage. The fungicidal composition has a technically feasible composition ratio, and shows a good synergistic effect on the prevention and treatment of grape downy mildew, and can effectively reduce the risk of resistance of grape downy mildew to dikestribin , is an ideal grape fungicide composition.

The invention discloses a bacillus amyloliquefaciens strain and applications of the bacillus amyloliquefaciens strain in inhibiting plasmopara viticola. The bacillus amyloliquefaciens KB provided by the invention is assigned with the accession number of CGMCC NO.8179. The cultured product of bacillus amyloliquefaciens KB is also within the protection range of the invention, and the fermented product of bacillus amyloliquefaciens KB is also within the protection range of the invention. The invention further protects the applications of the bacillus amyloliquefaciens KB, the cultured product of bacillus amyloliquefaciens KB, or the fermented product of bacillus amyloliquefaciens KB in inhibiting plasmopara viticola, in preventing and treating plasmopara viticola, and in preventing and treating plant diseases caused by plasmopara viticola. The invention provides the bacillus amyloliquefaciens strain having the inhibiting effect for plasmopara viticola, which has the great application value for preventing and treating plasmopara viticola.