The invention discloses a bactericidal composition containing picoxystrobin and famoxadone, and applications thereof, wherein the bactericidal composition uses picoxystrobin and famoxadone as main effective components, and a mass ratio of picoxystrobin to famoxadone is 1:50-70:1. According to the present invention, the bactericidal composition can be used for preventing and controlling the diseases of cereals and vegetables, has high synergistic effect, can overcome and delay the drug resistance of bacteria, has advantages of rapid sterilization, long effective period and application cost reducing, can provide the prevention and control effect significant superior to the use of the single agent, can be used for preventing and controlling fungal diseases on crops, can be particularly used for preventing and controlling leaf blight, leaf rust, glume blight, brown spot, powdery mildew, leaf spot, blight, Sclerotium disease, net blotch and leaf blotch of wheat, rice sheath blight disease,and powdery mildew, downy mildew, net blotch, rust, glume blight, late blight, leaf spot, anthracnose, stem blight, corky scab and other fungal diseases of pea, beets, rape, grapes, potatoes, melons,hot peppers and tomatoes, and can provide the effects superior to the use of the single agent.