The invention discloses a bactericidal composition containing picoxystrobin and prothioconazole and an application thereof. The bactericidal composition uses picoxystrobin and prothioconazole as main active ingredients, wherein the mass ratio of picoxystrobin to prothioconazole is 1:50-70:1. The bactericidal composition can be applied to the prevention and treatment of cereals, fruit trees, and vegetable diseases, has a high synergistic effect, overcomes and delays the drug resistance of pathogens, has fast sterilization speed, long-lasting effect, reduces application costs, and has good control effect. Significantly better than its single dose use. The present invention can be used to prevent and treat fungal diseases on crops, especially can be used to prevent and treat wheat leaf blight, leaf rust, glume blight, brown spot, powdery mildew, leaf spot, fusarium wilt, sclerotinia, nettle Spot and moire, rice sheath blight, rice false smut, rape and peanut gray mold, black spot, brown spot, black shank, sclerotinia and rust fungal diseases, the effect is better than single agent use.