6 results about "Wheat Rust" patented technology

The invention provides a multi-source feature dynamic weight wheat rust monitoring method with regional adaptability, and relates to the technical field of agricultural remote sensing monitoring. The method comprises the following steps: firstly, generating false missing points based on a buffer exclusion method, and constructing a monitoring data set with balanced positive and negative samples; secondly, combining the key phenological period of wheat, fusing multi-source data such as weather, soil, terrain and remote sensing vegetation index, and constructing an initial feature set reflecting the dynamic change of the environment; performing screening and dynamic weighting on the feature set by using a Lasso regression algorithm, and determining an optimal feature combination according to environmental differences of different regions; and finally, constructing a monitoring model by utilizing an integrated learning strategy and fusing multiple machine learning algorithms. The method can effectively solve the problems of difficulty in negative sample acquisition and poor cross-region mobility of the model in large-scale monitoring, and realizes high-precision dynamic monitoring of the suitable region of wheat rust.

The invention belongs to the technical field of gene engineering, and relates to application of wheat receptor-like kinase TaSPR12 in improvement of wheat disease resistance. The wheat receptor kinase gene TaSPR12 is analyzed and screened by using a reverse genetics method, and the gene is induced to express by stripe rust germs. According to the present invention, the CRISPR-Cas9 gene editing technology is adopted to knock out the wheat receptor kinase gene TaSPR12 to create the function deletion mutant, and the resistance of the wheat stripe rust main epidemic race is improved; the invention determines that the wheat receptor kinase gene TaSPR12 plays a negative regulation role in wheat stripe rust resistance, and can be used for genetic improvement of wheat rust resistance.

The invention belongs to the technical field of genetic engineering breeding, and provides application of a TaSnRK2.8 gene in regulation and control of wheat disease resistance. The nucleotide sequence of the TaSnRK2.8 gene is as shown in SEQ ID NO: 1, the gene encodes wheat cytoplasm serine / threonine protein kinase TaSnRK2.8, and the amino acid sequence of the TaSnRK2.8 is as shown in SEQ ID NO: 2. The over-expression of the TaSnRK2.8 gene can significantly enhance the resistance of wheat to stripe rust and weaken the pathogenicity of stripe rust; when the TaSnRK2.8 gene is knocked out, the disease resistance of the wheat is weakened, and the TaSnRK2.8 gene is proved to positively regulate the stripe rust resistance of the wheat. The invention provides important gene resources and technical support for genetic improvement of wheat rust resistance, and can be used for cultivating stripe rust resistant wheat varieties.

The invention provides an inoculation method convenient for wheat stripe rust single spore isolation, and belongs to the technical field of wheat stripe rust single spore isolation. Uniformly mixing the summer spore powder and the talcum powder according to a preset proportion, and adding the mixture into the L-shaped glass bent pipe; wax layers of bred wheat seedlings are removed, and the wheat seedlings are placed on a rotating table after being sprayed with water mist; the rotating table device is started to rotate uniformly; under the action of pressure, uniformly spraying the mixed bacterial powder in the L-shaped glass bent pipe to wheat seedling leaves for inoculation; wheat seedlings are subjected to isolated culture after being moisturized, and then single spore isolation is carried out. According to the method, the ratio of the summer spore powder to the talcum powder is accurately controlled, and an improved bacterium spraying device is adopted, so that the inoculation success rate and the spore distribution uniformity are remarkably improved, the separation effect is better, and single spores are easier to obtain.

The invention belongs to the technical field of biological prevention and control, provides application of arbutin to improvement of resistance of wheat to airborne diseases, and particularly provides application of arbutin to prevention and control of wheat stripe rust, leaf rust and powdery mildew. The arbutin is applied to improving the resistance of wheat to stripe rust, brown leaf rust and powdery mildew. The arbutin enhances the accumulation of wheat callose and improves the resistance of wheat to airborne diseases. The arbutin improves the expression quantity of the resistance genes TaPR1, TaPR2 and TaPR5, and improves the resistance of wheat to airborne diseases. Results of the invention show that arbutin can significantly enhance the resistance of wheat to leaf rust, stripe rust and powdery mildew. Fluorescent real-time quantitative PCR (Polymerase Chain Reaction) shows that after exogenous spraying of arbutin, the expression quantity of disease course defense reaction genes in wheat is obviously increased, and surface arbutin is a potential metabolite for enhancing wheat immunity and can be used for preventing and treating wheat rust.

This invention discloses TaMP Genes and their application in improving wheat rust resistance belong to the field of plant biotechnology and breeding. TaMP The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention utilizes Agrobacterium-mediated wheat embryo genetic transformation technology to prepare... TaMP Transgenic wheat plants with gene overexpression and knockout. It was verified that overexpression... TaMP Genes that reduce wheat's resistance to stripe rust; knockout TaMP The gene enhances wheat's resistance to stripe rust. Therefore, this invention verifies the efficacy of wheat myristylated modified protein. TaMP Genes play a crucial role in wheat's resistance to stripe rust. As mentioned above, TaMP This gene plays a negative regulatory role in the defense response of wheat against rust. It can be used to improve the disease resistance traits of wheat and other crops, and to breed new transgenic plant varieties resistant to disease.