16 results about "Wheat Rust" patented technology

The invention relates to application of a bactericidal composition to prevention and treatment of wheat rust, in particular to the bactericidal composition which comprises effective active ingredients of pyraclostrobin and cyproconazole, and is mainly characterized in that the weight ratio of pyraclostrobin to cyproconazole is 5: 1-1: 1, the composition is prepared into water dispersible granules, and the dosage of the effective active ingredients is 3-50g / hectare.

The invention provides a multi-source feature dynamic weight wheat rust monitoring method with regional adaptability, and relates to the technical field of agricultural remote sensing monitoring. The method comprises the following steps: firstly, generating false missing points based on a buffer exclusion method, and constructing a monitoring data set with balanced positive and negative samples; secondly, combining the key phenological period of wheat, fusing multi-source data such as weather, soil, terrain and remote sensing vegetation index, and constructing an initial feature set reflecting the dynamic change of the environment; performing screening and dynamic weighting on the feature set by using a Lasso regression algorithm, and determining an optimal feature combination according to environmental differences of different regions; and finally, constructing a monitoring model by utilizing an integrated learning strategy and fusing multiple machine learning algorithms. The method can effectively solve the problems of difficulty in negative sample acquisition and poor cross-region mobility of the model in large-scale monitoring, and realizes high-precision dynamic monitoring of the suitable region of wheat rust.

The present invention discloses a class of acyl imine derivatives containing trifluoromethyl oxadiazole as shown in the following general formula (I): for each substituent, see the specification. The present invention also discloses a preparation method and uses of the acyl imine derivatives containing trifluoromethyl oxadiazole. The acyl imine derivatives containing trifluoromethyl oxadiazole are particularly suitable for preventing and controlling soybean rust, wheat rust, corn rust, crop anthracnose, etc.

The present invention provides a wheat rust disease recognition method based on transfer learning and sharpness-aware minimization. 1. It is characterized by the following steps: S1: Obtain wheat rust disease pictures in different disease periods, and classify the wheat rust disease pictures according to the severity of the disease; S2: Preprocess and perform data augmentation on the wheat rust disease pictures to obtain a wheat rust disease training set; S3: Use the ImageNet large-scale picture dataset to pre-train a lightweight model to obtain a pre-trained model, and save the pre-trained model parameters; S4: On the basis of the pre-trained model, use the wheat rust disease training set to train the pre-trained model, adopt a label smoothing loss function, and combine the sharpness-aware minimization method to fine-tune the pre-trained model to improve the generalization ability of the pre-trained model. By introducing the transfer learning method, the present invention effectively improves the recognition accuracy of the lightweight model, and at the same time, combining the sharpness-aware minimization method can prevent the risk of model overfitting.

The invention belongs to the technical field of gene engineering, and relates to application of wheat receptor-like kinase TaSPR12 in improvement of wheat disease resistance. The wheat receptor kinase gene TaSPR12 is analyzed and screened by using a reverse genetics method, and the gene is induced to express by stripe rust germs. According to the present invention, the CRISPR-Cas9 gene editing technology is adopted to knock out the wheat receptor kinase gene TaSPR12 to create the function deletion mutant, and the resistance of the wheat stripe rust main epidemic race is improved; the invention determines that the wheat receptor kinase gene TaSPR12 plays a negative regulation role in wheat stripe rust resistance, and can be used for genetic improvement of wheat rust resistance.

The present invention discloses a trifluoromethyl oxadiazole derivative represented by general formula A-1: for each substituent, see the specification. The present invention also discloses a preparation method and uses of the trifluoromethyl oxadiazole derivative, and the trifluoromethyl oxadiazole derivative is particularly suitable for preventing and controlling soybean rust, wheat rust, corn rust, crop anthracnose, etc.

The present invention provides a wheat cell wall-associated receptor-like kinase TaWAKL8-2B and its application in rust resistance, belonging to the field of bio-agricultural technology. The present invention provides an application of wheat cell wall-associated receptor-like kinase TaWAKL8-2B in improving wheat rust resistance. The wheat cell wall-associated receptor-like kinase TaWAKL8-2B of the present invention can significantly enhance the broad-spectrum resistance of wheat to stripe rust and leaf rust by regulating linalool synthesis and ROS accumulation. Overexpression TaWAKL8-2B The disease resistance of transgenic plants was significantly improved, while knockout plants were susceptible to the disease. This invention provides an important gene resource for wheat rust resistance breeding.

The utility model relates to a wheat stripe rust inoculation device, and belongs to the technical field of wheat stripe rust inoculation. Comprising a cabinet body, a cabinet door is arranged on one side of the cabinet body, a moisturizing mechanism is arranged at the upper end of the cabinet body, rotating mechanisms are arranged at the upper end and the lower end of one side of the cabinet body, and the two rotating mechanisms are sleeved with bearing mechanisms. By arranging the bearing mechanism to be matched with the rotating mechanism, the height of the bearing mechanism can be adjusted, a user can put inoculated wheat into the bearing mechanism without bending down, the efficiency of putting the wheat into the cabinet body is also improved, the wheat in the bearing mechanism can be moisturized through the moisturizing mechanism, and the working efficiency of the user is improved. The wheat moves at a constant speed in the bearing mechanism, so that the wheat in each bearing box is uniformly moisturized, and the success rate of wheat stripe rust inoculation in the device is ensured.

Techniques are disclosed that enable generating a predicted yield for a cereal grain crop based on one or more traits extracted from image(s) of the cereal grain crop. Various implementations include determining a heading trait value based on the number of identified spikelets, where the spikelets are identified by processing the image(s) of the cereal grain crop using a spikelet detection model. Additional or alternative implementations include generating a predicted cereal grain crop yield based on one or more additional or alternative trait values such as one or more heading values, one or more projected leaf area values, one or more stand spacing values, one or more wheat rust values, one or more maturity detection values, one or more intercropping phenotyping values extracted cereal grains intercropped with other crops, one or more additional or alternative trait output values, and / or combinations thereof.

The utility model provides a portable sampling device, which relates to the technical field of field wheat rust sampling and comprises a sampling collection box and a sampling device. A left waistband is hinged to the left side face of the sampling and collecting box, a right waistband is hinged to the right side face of the sampling and collecting box, and the sampling device comprises a sampling box, a lower blade, a bearing pad, a turning cover, a torsion spring, an upper blade, a locking rod, a guide column, a connecting block and a traction spring. A lower blade is adhered to the interior of the sampling box; an upper blade is hinged to the top end of the turning cover; locking rods are welded to the left side face and the right side face of the connecting block, and a traction spring is welded to the upper side face of the connecting block; through the arrangement of the sampling device, the left waistband, the right waistband and the sampling and collecting box, a sampled sample can be conveniently carried about, the situation that germs make contact with the lost rust leaves through hands and infection is expanded is prevented, and the problem that the infection range of the rust disease in the field is expanded due to the contact type picking and sampling mode of the wheat rust leaves is solved.

The present invention belongs to the field of genetic engineering technology and relates to the application of wheat receptor-like kinase TaSRK1 in improving wheat disease resistance. The present invention uses reverse genetics methods to analyze and screen to obtain the wheat receptor kinase gene TaSRK1 , and proves that TaSRK1 is induced to express by stripe rust pathogens, and the CRISPR-Cas9 gene editing technology is used to knock out TaSRK1 to create a loss-of-function mutant, which shows improved resistance to the main prevalent races of wheat stripe rust, and determines that the wheat receptor kinase gene TaSRK1 plays a negative regulatory role in wheat resistance to stripe rust. The present invention patent can be used for genetic improvement of wheat rust resistance.

The invention belongs to the technical field of genetic engineering breeding, and provides application of a TaSnRK2.8 gene in regulation and control of wheat disease resistance. The nucleotide sequence of the TaSnRK2.8 gene is as shown in SEQ ID NO: 1, the gene encodes wheat cytoplasm serine / threonine protein kinase TaSnRK2.8, and the amino acid sequence of the TaSnRK2.8 is as shown in SEQ ID NO: 2. The over-expression of the TaSnRK2.8 gene can significantly enhance the resistance of wheat to stripe rust and weaken the pathogenicity of stripe rust; when the TaSnRK2.8 gene is knocked out, the disease resistance of the wheat is weakened, and the TaSnRK2.8 gene is proved to positively regulate the stripe rust resistance of the wheat. The invention provides important gene resources and technical support for genetic improvement of wheat rust resistance, and can be used for cultivating stripe rust resistant wheat varieties.

The invention provides an inoculation method convenient for wheat stripe rust single spore isolation, and belongs to the technical field of wheat stripe rust single spore isolation. Uniformly mixing the summer spore powder and the talcum powder according to a preset proportion, and adding the mixture into the L-shaped glass bent pipe; wax layers of bred wheat seedlings are removed, and the wheat seedlings are placed on a rotating table after being sprayed with water mist; the rotating table device is started to rotate uniformly; under the action of pressure, uniformly spraying the mixed bacterial powder in the L-shaped glass bent pipe to wheat seedling leaves for inoculation; wheat seedlings are subjected to isolated culture after being moisturized, and then single spore isolation is carried out. According to the method, the ratio of the summer spore powder to the talcum powder is accurately controlled, and an improved bacterium spraying device is adopted, so that the inoculation success rate and the spore distribution uniformity are remarkably improved, the separation effect is better, and single spores are easier to obtain.

The invention provides wheat cell wall related receptor-like kinase TaWAKL8-2B and application thereof in rust disease resistance, and belongs to the technical field of biological agriculture. The invention provides an application of a wheat cell wall related receptor-like kinase TaWAKL8-2B in improvement of wheat rust resistance. The wheat cell wall related receptor-like kinase TaWAKL8-2B disclosed by the invention can be used for remarkably enhancing the broad-spectrum resistance of wheat to puccinia striiformis and leaf rust by regulating and controlling the synthesis of linalool and the accumulation of ROS (reactive oxygen species). The disease resistance of the transgenic plant overexpressed with TaWAKL8-2B is obviously improved, and the plant is infected if the plant is knocked out. The invention provides an important gene resource for wheat anti-rust breeding.

The invention belongs to the technical field of biological prevention and control, provides application of arbutin to improvement of resistance of wheat to airborne diseases, and particularly provides application of arbutin to prevention and control of wheat stripe rust, leaf rust and powdery mildew. The arbutin is applied to improving the resistance of wheat to stripe rust, brown leaf rust and powdery mildew. The arbutin enhances the accumulation of wheat callose and improves the resistance of wheat to airborne diseases. The arbutin improves the expression quantity of the resistance genes TaPR1, TaPR2 and TaPR5, and improves the resistance of wheat to airborne diseases. Results of the invention show that arbutin can significantly enhance the resistance of wheat to leaf rust, stripe rust and powdery mildew. Fluorescent real-time quantitative PCR (Polymerase Chain Reaction) shows that after exogenous spraying of arbutin, the expression quantity of disease course defense reaction genes in wheat is obviously increased, and surface arbutin is a potential metabolite for enhancing wheat immunity and can be used for preventing and treating wheat rust.

This invention discloses TaMP Genes and their application in improving wheat rust resistance belong to the field of plant biotechnology and breeding. TaMP The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention utilizes Agrobacterium-mediated wheat embryo genetic transformation technology to prepare... TaMP Transgenic wheat plants with gene overexpression and knockout. It was verified that overexpression... TaMP Genes that reduce wheat's resistance to stripe rust; knockout TaMP The gene enhances wheat's resistance to stripe rust. Therefore, this invention verifies the efficacy of wheat myristylated modified protein. TaMP Genes play a crucial role in wheat's resistance to stripe rust. As mentioned above, TaMP This gene plays a negative regulatory role in the defense response of wheat against rust. It can be used to improve the disease resistance traits of wheat and other crops, and to breed new transgenic plant varieties resistant to disease.