13 results about "Callose" patented technology

The invention provides an OsWRKY19 gene and an application of the OsWRKY19 gene in resistance to cochliobolus miyabeanus of rice. A CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) technology is adopted to obtain an OsWRKY19 knockout mutant (Oswrky19) and an overexpression strain of And after the cochliobolus miyabeanus pathogenic bacteria are inoculated, compared with a wild type, the knockout mutant is more susceptible to diseases, and the over-expression strain resistance is obviously enhanced. The OsWRKY19 overexpression obviously improves the callose deposition level after rice plants are induced by chitin, and further enhances the basic defensive reaction of rice. RT-qPCR discovers that the expression of the defensive reaction related gene OsMBL1 in a knockout mutant is reduced and is increased in an overexpression plant, which indicates that the OsWRKY19 can promote the expression of the OsMBL1, thereby enhancing the resistance of rice. In conclusion, the OsWRKY19 is a gene for positively regulating and controlling the resistance of the cochliobolus miyabeanus of the rice, and can promote the basic defense response of the rice and the expression of a disease-resistant related gene, so that the resistance of the rice to the cochliobolus miyabeanus is improved. The content of the invention provides important genetic resources and theoretical guidance for the breeding of the cochliobolus miyabeanus resistant variety of rice.

The invention discloses an application of FGSG09291 protein and a coding gene thereof in improving plant disease resistance, and belongs to the technical field of plant immune induced resistance. According to the invention, a novel FGSG09291 protein with a plant immune activation function is found from fusarium graminearum, and the FGSG09291 protein can promote plant active oxygen outbreak and callose accumulation and provide basic immune response for plants; in addition, high expression of the PR1a gene, the WRKY70 gene and the LOX3 gene can be induced, the PR1a gene and the WRKY70 gene are genes of a salicylic acid pathway, and the increase of salicylic acid can improve the broad-spectrum resistance of plants. Experiments prove that the prevention and control effect of corn on dwarf mosaic disease can be enhanced by externally applying the FGSG09291 protein.

The application discloses a banana fusarium wilt secreted protein FoXYG1 and application thereof, and belongs to the technical field of molecular plant pathology. The secreted protein FoXYG1 can induce plant defense response, including causing plant cell hypersensitive necrosis, inducing callose accumulation and up-regulated expression of a plant immune-related pathway marker gene, activating SA, JA, ET and HR pathway-mediated defense pathways to induce plant immune response, and can significantly improve the resistance of plants to fusarium oxysporum and / or fusarium oxysporum. A new way is provided for improving plant disease resistance and reducing disease occurrence, and thus has a wide application prospect in the green development of modern agriculture.

The invention discloses an effect of an extracellular zinc metalloproteinase gene MoZMP1 in regulation and control of pathogenicity of magnaporthe oryzae. The full-length sequence of the MoZMP1 gene is as shown in SEQ ID NO.1, the cDNA sequence of the MoZMP1 gene is as shown in SEQ ID NO.2, and the amino acid sequence of the protein coded by the MoZMP1 gene is as shown in SEQ ID NO.3. According to the invention, a MoZMP1 gene is knocked out from magnaporthe oryzae, and a knockout mutant [delta] Mozmp1 is obtained. The analysis on the growth and development and pathogenicity of the knockout mutant delta Mozmp1 shows that the knockout of the MoZMP1 gene does not influence the growth and development process of the magnaporthe oryzae, but the pathogenicity of the magnaporthe oryzae is remarkably reduced, which indicates that the MoZMP1 gene can specifically regulate and control the virulence of the magnaporthe oryzae; the further analysis on the rice immunoreaction shows that a large amount of active oxygen and callose can be accumulated in the rice infected by the knockout mutant delta Mozmp1, and meanwhile, the expression of the rice defensive reaction gene is remarkably up-regulated, which indicates that the MoZMP1 gene has a remarkable effect on inhibiting the rice immunity and promoting the pathogenicity of the pyricularia oryzae.

The application belongs to the technical field of plant disease prevention and treatment, and particularly relates to application of salicylic acid in preparation of a preparation for preventing and treating sugarcane leaf blight. The application provides application of salicylic acid in preparation of a preparation for preventing and treating sugarcane leaf blight, fills the technical blank of salicylic acid in the field of disease prevention and treatment, and provides a green and efficient new prevention and treatment scheme. In the application, the salicylic acid can not only directly inhibit the mycelium growth of a pathogenic bacterium (sclerotinia roflsii), but also can induce the sugarcane plant to produce systemic acquired resistance (SAR) by activating the expression of a defense gene, the deposition of callose, a MAPK cascade reaction, phenylalanine ammonia lyase and peroxidase activity, so as to realize the double prevention and control effects of direct bacteriostasis and immune induction.

The application discloses a banana burrowing nematode branch acid mutase and application thereof. The application provides application of a banana burrowing nematode branch acid mutase gene RsCM in prevention and treatment of banana burrowing nematodes, and the nucleotide sequence of the banana burrowing nematode branch acid mutase gene RsCM is shown in SEQ ID NO. 1 or SEQ ID NO. 2. Tobacco transient expression shows that RsCM can significantly inhibit flg22-induced defense gene expression and callose deposition, and can significantly inhibit BAX-induced cell necrosis, which indicates that RsCM can inhibit the defense response of plants; a transgenic tomato is constructed through in planta RNAi technology, and it is found that the pathogenicity of banana burrowing nematodes is reduced after inoculation of the nematodes. The application provides a new strategy for prevention and treatment of nematodes in agricultural production, provides a new target for cultivation of nematode-resistant plant varieties, and provides a new approach for prevention and treatment of banana burrowing nematodes.

This invention belongs to the field of biopesticide technology and discloses a plant immune inducer containing cyclic diguanylic acid (cDGA) and its application. The final concentration of cDGA in this plant immune inducer is 0.5~1 μmol / L. After spraying, it can rapidly activate multiple immune responses in plants, including reactive oxygen species (ROS) bursts, callose accumulation, and efficient expression of disease resistance genes and ROS scavenging-related genes. By activating the plant's own immune system, it enhances the plant's resistance to various pathogens, showing significant control effects against bacterial spot fungus of tomato, Phytophthora, and Actinidia kiwifruit canker. This cDGA plant immune inducer also has advantages such as being environmentally friendly, low in toxicity, residue-free, and unlikely to induce drug resistance in pathogens, thus having broad application prospects.

The invention discloses a rice disease resistance related gene OsSIE3 and application thereof. The nucleotide sequence of the OsSIE3 gene is as shown in SEQ ID NO. 1. The invention preliminarily analyzes the biological function of the OsSIE3 gene, and particularly relates to the application of the OsSIE3 gene in regulation of rice blast resistance, regulation of rice immune response and regulation of rice growth and development. The OsSIE3 gene in wild type rice is knocked out through a CRISPR-Cas9 gene editing technology, an OsSIE3 gene knockout mutant is obtained, and the mutant shows that the resistance to rice blast is reduced, the callose deposition amount is reduced, and the grain width, the grain thickness and the thousand grain weight are reduced. It is indicated that the OsSIE3 gene is crucial to maintenance of rice disease resistance, normal immune response and excellent grain traits, and the gene not only has important production and application potentials, but also can be used for research of a rice blast resistance mechanism, an immune response regulation mechanism and a growth and development regulation mechanism of rice.

The invention belongs to the technical field of biological prevention and control, provides application of arbutin to improvement of resistance of wheat to airborne diseases, and particularly provides application of arbutin to prevention and control of wheat stripe rust, leaf rust and powdery mildew. The arbutin is applied to improving the resistance of wheat to stripe rust, brown leaf rust and powdery mildew. The arbutin enhances the accumulation of wheat callose and improves the resistance of wheat to airborne diseases. The arbutin improves the expression quantity of the resistance genes TaPR1, TaPR2 and TaPR5, and improves the resistance of wheat to airborne diseases. Results of the invention show that arbutin can significantly enhance the resistance of wheat to leaf rust, stripe rust and powdery mildew. Fluorescent real-time quantitative PCR (Polymerase Chain Reaction) shows that after exogenous spraying of arbutin, the expression quantity of disease course defense reaction genes in wheat is obviously increased, and surface arbutin is a potential metabolite for enhancing wheat immunity and can be used for preventing and treating wheat rust.

The invention relates to the field of plants, in particular to a GhICDH3 gene and application of the GhICDH3 gene in regulation and control of verticillium wilt resistance of plants. The invention provides an application of a GhICDH3 gene or a GhICDH3 protein coded by the GhICDH3 gene in any of the following items: an application in improvement of verticillium wilt resistance of plants; or the application in cultivating new germplasm with high verticillium wilt resistance. According to the invention, sequence characteristics, protein subcellular localization and ubiquitination modification site functions of the cotton GhICDH3 gene are systematically analyzed, and a disease-resistant mechanism of the gene for regulating reactive oxygen species outbreak, callose accumulation and lignin synthesis of cotton and an action relationship between the gene and interaction protein GhHSP70 are determined; a novel target gene capable of being used for genetic improvement of cotton verticillium wilt resistance is excavated, and the core technical problems that in cotton verticillium wilt resistance research, high-quality anti-disease genes are deficient, a key regulation mechanism is unknown, and efficient molecular breeding is difficult to achieve are solved.

The application discloses application of FGSG_09291 protein and a coding gene thereof in improving plant disease resistance, and belongs to the technical field of plant immune induction. A new FGSG_09291 protein with plant immune activation function is found from Fusarium graminearum, and the FGSG_09291 protein can promote active oxygen burst and callose accumulation of plants, and provide basic immune response for the plants. PR1a genes, WRKY70 genes and LOX3 high expression of the genes, PR1a and WRKY70 the genes are genes of a salicylic acid pathway, and increase of the salicylic acid can improve broad-spectrum resistance of the plants. It is verified through experiments that the FGSG_09291 protein can enhance the prevention and control effect of corn on the stunt disease.

The application relates to application of a cotton class sweet protein gene GhTLP1b in improving resistance of cotton to verticillium wilt. The application clones a GhTLP1b gene from a disease-resistant material ZZM2, uses an agrobacterium-mediated transformation technology to obtain transgenic cotton strains of gene overexpression and gene silencing of the gene, and proves that the GhTLP1b overexpression strain enhances the resistance of cotton to the verticillium wilt, and is characterized by delayed disease occurrence and reduced disease index; the GhTLP1b silencing strain makes the cotton susceptible, and is characterized by weakened active oxygen burst and reduced lignin and callose accumulation. The application identifies and verifies an important resistance gene of cotton to the verticillium wilt, and provides an effective gene resource for genetic breeding.

The invention relates to application of a cotton sweet protein gene GhTLP1b in improvement of verticillium wilt resistance of cotton. A GhTLP1b gene is cloned from a disease-resistant material ZZM2, an agrobacterium-mediated transformation technology is utilized to obtain a transgenic cotton strain with overexpression and gene silencing of the gene, and it is proved that the GhTLP1b overexpression strain enhances the resistance of cotton to verticillium wilt, which is represented by disease delay and disease index reduction; the GhTLP1b silencing strain causes cotton susceptibility, which shows that active oxygen bursting is weakened, and lignin and callose accumulation is reduced. The invention identifies and verifies the important resistance gene of cotton verticillium wilt resistance, and provides effective gene resources for genetic breeding.