24 results about "Callose" patented technology

The invention relates to the technical field of biological prevention and control, in particular to a method for analyzing rice leaf rhythm change after rice is infected by pyricularia grisea and application of the method in accurate prevention and control of rice blast. The analysis method comprises the following steps: (1) spraying a pyricularia grisea spore suspension on rice leaves; (2) carrying out whole-genome transcriptome analysis on the rice leaves, and carrying out clustering heat map; (3) analyzing expression conditions of the biological clock genes and the phytochrome related genes in the circadian rhythm pathway of the rice leaves, and analyzing the biological clock genes and the phytochrome related genes by a MetaCycle method; and (4) analyzing the deposition amount of H2O2 and callose in the rice leaves. Determining the dynamic change rule of the rice immune process caused by magnaporthe oryzae infection according to the method; in combination with the characteristics of small molecules, small-molecule pesticides are reasonably applied at a specific time point aiming at the rice blast, so that efficient and accurate prevention and control of the rice blast are realized.

The invention provides an OsWRKY19 gene and an application of the OsWRKY19 gene in resistance to cochliobolus miyabeanus of rice. A CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) technology is adopted to obtain an OsWRKY19 knockout mutant (Oswrky19) and an overexpression strain of And after the cochliobolus miyabeanus pathogenic bacteria are inoculated, compared with a wild type, the knockout mutant is more susceptible to diseases, and the over-expression strain resistance is obviously enhanced. The OsWRKY19 overexpression obviously improves the callose deposition level after rice plants are induced by chitin, and further enhances the basic defensive reaction of rice. RT-qPCR discovers that the expression of the defensive reaction related gene OsMBL1 in a knockout mutant is reduced and is increased in an overexpression plant, which indicates that the OsWRKY19 can promote the expression of the OsMBL1, thereby enhancing the resistance of rice. In conclusion, the OsWRKY19 is a gene for positively regulating and controlling the resistance of the cochliobolus miyabeanus of the rice, and can promote the basic defense response of the rice and the expression of a disease-resistant related gene, so that the resistance of the rice to the cochliobolus miyabeanus is improved. The content of the invention provides important genetic resources and theoretical guidance for the breeding of the cochliobolus miyabeanus resistant variety of rice.

The invention discloses an application of FGSG09291 protein and a coding gene thereof in improving plant disease resistance, and belongs to the technical field of plant immune induced resistance. According to the invention, a novel FGSG09291 protein with a plant immune activation function is found from fusarium graminearum, and the FGSG09291 protein can promote plant active oxygen outbreak and callose accumulation and provide basic immune response for plants; in addition, high expression of the PR1a gene, the WRKY70 gene and the LOX3 gene can be induced, the PR1a gene and the WRKY70 gene are genes of a salicylic acid pathway, and the increase of salicylic acid can improve the broad-spectrum resistance of plants. Experiments prove that the prevention and control effect of corn on dwarf mosaic disease can be enhanced by externally applying the FGSG09291 protein.

This disclosure provides a method for the VdGH45-1 protein to activate and induce plant immune responses, which can efficiently induce immune responses such as ROS burst, callus deposition, and expression of resistance-related genes in plants, while significantly enhancing plant resistance, providing a new approach to improving plant resistance.

The application discloses a banana fusarium wilt secreted protein FoXYG1 and application thereof, and belongs to the technical field of molecular plant pathology. The secreted protein FoXYG1 can induce plant defense response, including causing plant cell hypersensitive necrosis, inducing callose accumulation and up-regulated expression of a plant immune-related pathway marker gene, activating SA, JA, ET and HR pathway-mediated defense pathways to induce plant immune response, and can significantly improve the resistance of plants to fusarium oxysporum and / or fusarium oxysporum. A new way is provided for improving plant disease resistance and reducing disease occurrence, and thus has a wide application prospect in the green development of modern agriculture.

The invention discloses an effect of an extracellular zinc metalloproteinase gene MoZMP1 in regulation and control of pathogenicity of magnaporthe oryzae. The full-length sequence of the MoZMP1 gene is as shown in SEQ ID NO.1, the cDNA sequence of the MoZMP1 gene is as shown in SEQ ID NO.2, and the amino acid sequence of the protein coded by the MoZMP1 gene is as shown in SEQ ID NO.3. According to the invention, a MoZMP1 gene is knocked out from magnaporthe oryzae, and a knockout mutant [delta] Mozmp1 is obtained. The analysis on the growth and development and pathogenicity of the knockout mutant delta Mozmp1 shows that the knockout of the MoZMP1 gene does not influence the growth and development process of the magnaporthe oryzae, but the pathogenicity of the magnaporthe oryzae is remarkably reduced, which indicates that the MoZMP1 gene can specifically regulate and control the virulence of the magnaporthe oryzae; the further analysis on the rice immunoreaction shows that a large amount of active oxygen and callose can be accumulated in the rice infected by the knockout mutant delta Mozmp1, and meanwhile, the expression of the rice defensive reaction gene is remarkably up-regulated, which indicates that the MoZMP1 gene has a remarkable effect on inhibiting the rice immunity and promoting the pathogenicity of the pyricularia oryzae.

The application belongs to the technical field of plant disease prevention and treatment, and particularly relates to application of salicylic acid in preparation of a preparation for preventing and treating sugarcane leaf blight. The application provides application of salicylic acid in preparation of a preparation for preventing and treating sugarcane leaf blight, fills the technical blank of salicylic acid in the field of disease prevention and treatment, and provides a green and efficient new prevention and treatment scheme. In the application, the salicylic acid can not only directly inhibit the mycelium growth of a pathogenic bacterium (sclerotinia roflsii), but also can induce the sugarcane plant to produce systemic acquired resistance (SAR) by activating the expression of a defense gene, the deposition of callose, a MAPK cascade reaction, phenylalanine ammonia lyase and peroxidase activity, so as to realize the double prevention and control effects of direct bacteriostasis and immune induction.

The invention provides a Huanglongbing prediction method for analyzing the content of the callose of the citrus vein duct through microspectrum, which comprises the following steps of: detecting the change of the callose by adopting a microspectrum image aiming at the callose of the citrus vein duct through a spectrum image method, and analyzing whether a plant has a defensive reaction of Huanglongbing infection so as to predict the Huanglongbing infection of the plant; according to the invention, the early diagnosis effect is better, and the accuracy is higher.

The invention belongs to the technical field of bioengineering, and particularly relates to a protein elicitor SyCD1 and application thereof. According to the invention, a protein elicitor SyCD1 gene is obtained by cloning from Rabdolothrix poplar Hhs.015, the nucleotide sequence of the protein elicitor SyCD1 gene is as shown in SEQ ID No: 1, and the amino acid sequence of the protein coded by the protein elicitor SyCD1 gene is as shown in SEQ ID No: 2. The efficacy of the unknown functional protein-protein elicitor SyCD1 is discussed and studied for the first time, the unknown functional protein-protein elicitor SyCD1 is applied to improvement of plant disease resistance, and tests prove that the SyCD1 protein induces plant active oxygen accumulation and callose bursting, regulates plant defense gene expression and improves plant disease resistance. The invention provides a new biological material for improving the disease resistance of plants.

The application belongs to the technical field of bioengineering and particularly relates to a protein elicitor SyCD1 and application thereof. SyCD1 The nucleotide sequence of the gene is shown as SEQ ID No:1, and the amino acid sequence of the encoded protein is shown as SEQ ID No:2. The efficacy of the unknown function protein-protein elicitor SyCD1 is first explored in the application, and the protein is applied to improving the disease resistance of plants. It is verified through experiments that the SyCD1 protein induces the accumulation of active oxygen and the eruption of callose in plants, regulates the expression of plant defense genes, and improves the disease resistance of plants. The application provides a new biological material for improving the disease resistance of plants.

The application discloses a banana burrowing nematode branch acid mutase and application thereof. The application provides application of a banana burrowing nematode branch acid mutase gene RsCM in prevention and treatment of banana burrowing nematodes, and the nucleotide sequence of the banana burrowing nematode branch acid mutase gene RsCM is shown in SEQ ID NO. 1 or SEQ ID NO. 2. Tobacco transient expression shows that RsCM can significantly inhibit flg22-induced defense gene expression and callose deposition, and can significantly inhibit BAX-induced cell necrosis, which indicates that RsCM can inhibit the defense response of plants; a transgenic tomato is constructed through in planta RNAi technology, and it is found that the pathogenicity of banana burrowing nematodes is reduced after inoculation of the nematodes. The application provides a new strategy for prevention and treatment of nematodes in agricultural production, provides a new target for cultivation of nematode-resistant plant varieties, and provides a new approach for prevention and treatment of banana burrowing nematodes.

The present application relates to a kind of complex microbial inoculant for preventing and treating citrus Huanglongbing and its preparation method and application.The complex microbial inoculant for preventing and treating citrus Huanglongbing includes 5-10 parts of Bacillus subtilis X1, 5-10 parts of Bacillus pumilus X2, 5-10 parts of Bacillus licheniformis X3, 5-10 parts of Bacillus amyloliquefaciens X4 and 5-10 parts of Pseudomonas fluorescens X5 by weight.After adding the complex microbial inoculant, the content of callose in the diseased leaf can be effectively reduced, the mechanism of self-resistance of plant is induced, the disease resistance is improved, and the Huanglongbing bacteria has obvious inhibitory effect and growth promotion effect.

The invention relates to an application of LtSCRE11 protein in regulating and controlling resistance of peach trees to gummosis. Micromolecular cysteine-rich protein LtSCRE11 secreted by peach gummosis pathogenic bacteria, namely, dispora pirocolor, is screened by a method of combining a genome and a plant bacterium interaction transcriptome with bioinformatics. It is found through injection of nicotiana benthamiana that LtSCRE11 can induce ROS bursting and callus accumulation, and injection of purified LtSCRE11 protein into nicotiana benthamiana and peaches can induce expression of plant immune related genes and MAPK protein phosphorylation signals, so that resistance of nicotiana benthamiana and peaches to pathogenic bacteria is induced. The invention proves that the LtSCRE11 plays a positive role in coping with the infection of pathogenic bacteria by plants, the resistance of peaches to the pathogenic bacteria of the gummosis can be remarkably improved by injecting the LtSCRE11 protein into leaves of peach trees, and the LtSCRE11 protein is expected to be developed into a novel immune resistance inducer target for preventing and controlling the gummosis of the peach trees.

The invention relates to the technical field of gene engineering, in particular to application of a GhUGP1 gene in regulation and control of drought resistance of cotton. Phylogenetic analysis reveals that the GhUGP1 gene has high conservative property in the plant evolution process, and promoter cis-acting element analysis shows that the GhUGP1 gene contains rich stress response elements and indicates that the GhUGP1 gene participates in abiotic stress response. By constructing a GhUGP1 overexpression plant, it is found that the GhUGP1 overexpression plant shows a more drought-resistant phenotype, a lower in-vitro leaf water loss rate and a faster rehydration recovery capability under drought stress. Physiological index detection shows that active oxygen accumulation of overexpressed plants is remarkably reduced, antioxidant enzyme activity is improved, malondialdehyde content is reduced, callose deposition is reduced, and pore opening is reduced. Results show that the GhUGP1 plays a key role in maintaining moisture balance, enhancing active oxygen scavenging ability and regulating stomatal movement by positively regulating the drought resistance of the cotton.

This invention belongs to the field of biopesticide technology and discloses a plant immune inducer containing cyclic diguanylic acid (cDGA) and its application. The final concentration of cDGA in this plant immune inducer is 0.5~1 μmol / L. After spraying, it can rapidly activate multiple immune responses in plants, including reactive oxygen species (ROS) bursts, callose accumulation, and efficient expression of disease resistance genes and ROS scavenging-related genes. By activating the plant's own immune system, it enhances the plant's resistance to various pathogens, showing significant control effects against bacterial spot fungus of tomato, Phytophthora, and Actinidia kiwifruit canker. This cDGA plant immune inducer also has advantages such as being environmentally friendly, low in toxicity, residue-free, and unlikely to induce drug resistance in pathogens, thus having broad application prospects.

The invention discloses a rice disease resistance related gene OsSIE3 and application thereof. The nucleotide sequence of the OsSIE3 gene is as shown in SEQ ID NO. 1. The invention preliminarily analyzes the biological function of the OsSIE3 gene, and particularly relates to the application of the OsSIE3 gene in regulation of rice blast resistance, regulation of rice immune response and regulation of rice growth and development. The OsSIE3 gene in wild type rice is knocked out through a CRISPR-Cas9 gene editing technology, an OsSIE3 gene knockout mutant is obtained, and the mutant shows that the resistance to rice blast is reduced, the callose deposition amount is reduced, and the grain width, the grain thickness and the thousand grain weight are reduced. It is indicated that the OsSIE3 gene is crucial to maintenance of rice disease resistance, normal immune response and excellent grain traits, and the gene not only has important production and application potentials, but also can be used for research of a rice blast resistance mechanism, an immune response regulation mechanism and a growth and development regulation mechanism of rice.

The invention belongs to the technical field of biological prevention and control, provides application of arbutin to improvement of resistance of wheat to airborne diseases, and particularly provides application of arbutin to prevention and control of wheat stripe rust, leaf rust and powdery mildew. The arbutin is applied to improving the resistance of wheat to stripe rust, brown leaf rust and powdery mildew. The arbutin enhances the accumulation of wheat callose and improves the resistance of wheat to airborne diseases. The arbutin improves the expression quantity of the resistance genes TaPR1, TaPR2 and TaPR5, and improves the resistance of wheat to airborne diseases. Results of the invention show that arbutin can significantly enhance the resistance of wheat to leaf rust, stripe rust and powdery mildew. Fluorescent real-time quantitative PCR (Polymerase Chain Reaction) shows that after exogenous spraying of arbutin, the expression quantity of disease course defense reaction genes in wheat is obviously increased, and surface arbutin is a potential metabolite for enhancing wheat immunity and can be used for preventing and treating wheat rust.

The invention relates to the field of plants, in particular to a GhICDH3 gene and application of the GhICDH3 gene in regulation and control of verticillium wilt resistance of plants. The invention provides an application of a GhICDH3 gene or a GhICDH3 protein coded by the GhICDH3 gene in any of the following items: an application in improvement of verticillium wilt resistance of plants; or the application in cultivating new germplasm with high verticillium wilt resistance. According to the invention, sequence characteristics, protein subcellular localization and ubiquitination modification site functions of the cotton GhICDH3 gene are systematically analyzed, and a disease-resistant mechanism of the gene for regulating reactive oxygen species outbreak, callose accumulation and lignin synthesis of cotton and an action relationship between the gene and interaction protein GhHSP70 are determined; a novel target gene capable of being used for genetic improvement of cotton verticillium wilt resistance is excavated, and the core technical problems that in cotton verticillium wilt resistance research, high-quality anti-disease genes are deficient, a key regulation mechanism is unknown, and efficient molecular breeding is difficult to achieve are solved.

The application discloses application of FGSG_09291 protein and a coding gene thereof in improving plant disease resistance, and belongs to the technical field of plant immune induction. A new FGSG_09291 protein with plant immune activation function is found from Fusarium graminearum, and the FGSG_09291 protein can promote active oxygen burst and callose accumulation of plants, and provide basic immune response for the plants. PR1a genes, WRKY70 genes and LOX3 high expression of the genes, PR1a and WRKY70 the genes are genes of a salicylic acid pathway, and increase of the salicylic acid can improve broad-spectrum resistance of the plants. It is verified through experiments that the FGSG_09291 protein can enhance the prevention and control effect of corn on the stunt disease.

The invention discloses a nano antibody specifically bound with callose or an antigen binding fragment thereof, the callose nano antibody provided by the invention can be specifically bound with callose and has a wide application prospect in identification of plant immunity, the antibody purification process is relatively simple, the production cost is lower, and the method is suitable for industrial production. Wide application potential and technical advantages can be shown in biological scientific research.

This invention belongs to the field of biotechnology and discloses a method for immunofluorescence staining of proteins in Lycoris radiata meiotic cells. The method includes the following steps: 1) treating fixed anthers to obtain pollen mother cells in the meiotic stage; 2) treating with enzymes to decompose the cytoplasm of the meiotic cells; 3) preparing slides and performing fluorescent staining on the treated meiotic cells; 4) observing the localization signal of the target protein under a fluorescence microscope. This invention uses dextranase and other enzymes to treat pollen mother cells, effectively decomposing the thick outer callosity protective layer of Lycoris radiata pollen mother cells, which is beneficial for antibody binding to chromosomes; at the same time, it improves the slide preparation method, effectively enhancing the expression of fluorescence signals. This method is of great significance for studying the subcellular localization of proteins with different functions in Lycoris radiata pollen mother cells in the meiotic stage using immunofluorescence localization technology.

The application relates to application of a cotton class sweet protein gene GhTLP1b in improving resistance of cotton to verticillium wilt. The application clones a GhTLP1b gene from a disease-resistant material ZZM2, uses an agrobacterium-mediated transformation technology to obtain transgenic cotton strains of gene overexpression and gene silencing of the gene, and proves that the GhTLP1b overexpression strain enhances the resistance of cotton to the verticillium wilt, and is characterized by delayed disease occurrence and reduced disease index; the GhTLP1b silencing strain makes the cotton susceptible, and is characterized by weakened active oxygen burst and reduced lignin and callose accumulation. The application identifies and verifies an important resistance gene of cotton to the verticillium wilt, and provides an effective gene resource for genetic breeding.

The application provides a Verticillium dahliae exoprotein elicitor VdSP8, and finds that VdSP8 can improve plant resistance and induce plant defense response, heterologous expression of VdSP8 significantly improves the resistance of host plants to multiple pathogenic bacteria, including Verticillium dahliae, Botrytis cinerea and Pseudomonas syringae; meanwhile, VdSP8 can also improve plant growth rate, increase fresh weight and promote plant bolting. The results of cytological studies show that VdSP8 improves the resistance of plants to Verticillium dahliae by enhancing callose deposition, promoting H2O2 accumulation and regulating hypersensitive response, and spraying VdSP8 protein on plants or introducing VdSP8 gene into plant cells to express VdSP8 protein in the plant body can significantly improve the resistance of the plants to Verticillium dahliae, and has good agricultural application prospect.

The invention relates to application of a cotton sweet protein gene GhTLP1b in improvement of verticillium wilt resistance of cotton. A GhTLP1b gene is cloned from a disease-resistant material ZZM2, an agrobacterium-mediated transformation technology is utilized to obtain a transgenic cotton strain with overexpression and gene silencing of the gene, and it is proved that the GhTLP1b overexpression strain enhances the resistance of cotton to verticillium wilt, which is represented by disease delay and disease index reduction; the GhTLP1b silencing strain causes cotton susceptibility, which shows that active oxygen bursting is weakened, and lignin and callose accumulation is reduced. The invention identifies and verifies the important resistance gene of cotton verticillium wilt resistance, and provides effective gene resources for genetic breeding.