48 results about "Callose" patented technology

The invention discloses a meloidogyne graminicola translation elongation factor Mg-eFF1A and the application thereof to the control of plant diseases. A genomic sequence of the Mg-eFF1A is as shown inSEQ ID NO.1, and an amino acid sequence is as shown in SEQ ID NO.2. The Mg-eFF1A is firstly discovered, the fact that the Mg-eFF1A can be secreted to the exterior of body of meloidogyne graminicola,and the Mg-eFF1A can activate an immune system of a host during invasion of plant-parasitic nematodes, induce the fundamental immune response of paddies and disease resistance to pathogen, activate PTI immune response, such as pathogenesis-related gene expression, callose accumulation and MAP kinase phosphorylation, of the paddies, and enhance the resistance of the paddies to meloidogyne graminicola and magnaporthe grisea. In addition, researches find that the Mg-eFF1A can induce plant OsCERK1 expression during invasion of the meloidogyne graminicola, and the researches open a direction and strategy with huge potential for the prevention and control of the plant-parasitic nematodes.

The present invention relates to a polypeptide, or a fragment thereof, capable of enhancing callose biosynthesis and / or accumulation, wherein at least one of the conserved amino acid residues selected from the group consisting of residue corresponding to R84 of SEQ ID NO: 1, residue corresponding to R1926 or SEQ ID NO: 1 and residue corresponding to P189 of SEQ ID NO: 1, of the polypeptide or a fragment thereof, is modified by a mutation selected from the group consisting of substitution and deletion.

A method of treating cotton comprising: (i) contacting cotton with an aqueous wash liquor comprising water and an endo-β-1,3-glucanase enzyme; (ii) optionally rinsing the cotton; and (iii) drying the cotton. The wash liquor may contain surfactant. The cotton may be contacted with the aqueous wash liquor with agitation. The method may be for removal of callose from cotton. The method may be for the manufacture of a cotton-containing fabric having improved whiteness maintenance, soil repellency, reduced malodour, anti-wrinkle benefits, and / or improved drying.

Provided herein is Bacillus amyloliquefaciens strain PMB05 used in the field of plant disease control. Inoculation of plants with the strain can increase immune responses induced by detection of fungi and / or bacteria in plant, for example, increase the production of ROS and deposition of callose. Also, the strain has the effect of promoting plant growth. Therefore, the strain and its culture filtrate of the present invention can boost plant disease resistance and promote plant growth, which can be extensively used to control plant diseases and have the potential of turning into a commodified biological control agent.

The present invention "a trichoderma hydrophobin Thfb6 and its strain, plant ISR elicitor and excitation method" belongs to the field of molecular biology. The amino acid sequence of the Trichoderma hydrophobin Thfb6 is shown in SEQ ID NO.1. Based on the Trichoderma hydrophobin Thfb6, the present invention also provides a plant ISR elicitor and its stimulating method, a TMV resistance inducer and its inducing method. The trichoderma hydrophobin Thfb6 of the present invention can cause active oxygen bursts in plant leaves, induce cell extracellular alkalization, callose and phenolic substance accumulation and other early defense reactions, and improve stress resistance enzyme activities such as PAL, POD and PPO in leaves Improve and induce the expression of disease process-related protein gene PR‑1b, systemic resistance marker gene NPR1 and defense response key enzyme gene PAL, and induce tobacco to improve disease resistance to tobacco mosaic disease, and at the same time induce cucumber resistance to Fusarium wilt sex.

The invention belongs to the field of agricultural biotechnology, and in particular relates to the cotton Verticillium wilt resistance related gene GhABC and its encoded protein and application. The nucleotide sequence of the cotton verticillium wilt resistance-related gene GhABC of the present invention is shown in SEQ ID No.2, and the amino acid sequence of its encoded protein is shown in SEQ ID No.1. After the gene silencing plants are inoculated with the spore suspension of the cotton Verticillium dahliae pathogen Vd080, the silencing plants are more susceptible to the disease. Through mechanism research, it is found that the synthesis of xylem and callose in the silencing plants decreases, the ROS decreases, and the expression of some defense genes is down-regulated. It proved that GhABC is positively correlated with cotton resistance to Verticillium wilt, and GhABC can be used as a candidate gene for cotton breeding for disease resistance.