Mutant male sterile gene of soybean
A male sterility gene, male sterility technology, applied in the direction of plant genetic improvement, angiosperm/flowering plants, botanical equipment and methods, etc., can solve problems such as increased cost efficiency
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Embodiment 1
[0026] The following examples are provided to further illustrate the present invention and are not intended to limit the invention further than that set forth in the appended claims. Example 1 Demonstration of male sterility in ms mutant soybean lines
[0027] Seeds of the male sterile line msMOS were obtained from Midwest Oilseeds, Adel, Iowa. This line has an unusually high yield in the field. To test the integrity of male sterility, 300 plants of progeny of known heterozygotes were grown in the Iowa State University, Ames greenhouse in the summer of 1995 in the absence of insect pollinators. During anthesis, plants were classified as fertile and sterile based on the presence or absence of pollen. Fertile plants were de-weeded. The male sterile plants were saved and the number of seeds set was checked one month after the plants had fully flowered.
[0028] In greenhouse experiments in the absence of insect pollinators, no pods formed in the male sterile plants, indicatin...
Embodiment 2
[0028] In greenhouse experiments in the absence of insect pollinators, no pods formed in the male sterile plants, indicating that this was a completely male sterile line, and that the summer greenhouse environment (June to August 1995) did not affect the male sterile gene expression. Genetic analysis of embodiment 2 ms mutant soybean lines
[0029] To determine whether the mutation is a cytoplasmic male sterile mutant, a genetic study was performed. To determine whether new mutations arose at novel loci, or independent mutations occurred at one of the previously described loci shown in Table 1 (ms1, ms2, ms3, ms4, ms5 or ms6), allelism tests were performed. Crosses were performed using a known regressive sterile homozygous as the female parent and an F1 hybrid (hybrid) from Midwest Oilseeds as the male parent. F1 seeds were planted in the greenhouse at Iowa State University, Ames, or at the University of Puerto Rico Soybean Breeding Facility (Isabela Substation, Isabela, Pue...
Embodiment 3
[0038] Linkage was tested between the ms and W1 (flower color) loci, and X was calculated using P=0.45 2 = 2.65, and for an independent classification between the msms and T1 (pilose color) loci, X 2 =0.30 and P=0.96. In both tests, the observed values corresponded to the expected ratio of 9:3:3:1. There was no linkage between the ms and W1 loci or between the ms and T1 loci. Example 3 Cytological analysis of anther development in male sterile soybean
[0039] Cytological observations of anther and pollen development were obtained from regenerated shoots of various sizes collected from male-fertile and male-sterile plants. in I 2 Identification of male fertile and male sterile plants by anthers in the late flattening stage in an aqueous solution of K1 (Jensen, W.A., Phytohistochemistry, Freeman, San Francisco, p. 203, 1962); anthers from male fertile plants exhibit densely stained pollen grains , while anthers from male sterile plants were nearly empty, with only a few ...
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