19 results about "ANILINE BLUE" patented technology

The invention discloses a method for nondestructively detecting the content of beta-1, 3-glucan in ganoderma mycelium, and relates to the technical field of biological detection, and the method comprises the following steps: preparing different varieties of ganoderma mycelium samples; determining the content of beta-1, 3-glucan in different varieties of ganoderma mycelium samples by adopting an aniline blue fluorescence method; acquiring near infrared spectrum data of different varieties of ganoderma mycelia; preprocessing the spectral data; screening characteristic wavelengths; establishing a prediction model; and predicting by using the model. According to the prediction model for detecting the content of the beta-1, 3-glucan in the ganoderma mycelium based on the near infrared spectrum technology and the fluorescent quantitative technology, the content of the beta-1, 3-glucan can be rapidly and nondestructively detected at high flux, the prediction effect is good, the determination time is short, and the prediction model has the advantages of being easy and convenient to operate, rapid, low in cost and the like.

The invention relates to a compound for inhibiting osteosarcoma cell invasion and metastasis capability and application of the compound, in particular to application of a PAC channel inhibitor to preparation of a composition or a preparation, and the composition or the preparation is used for preventing and / or treating osteosarcoma. It is found for the first time that PAC channel inhibitors (such as Evans blue (EB), trypan blue (TB), aniline blue (AB), Coomassie brilliant blue (CBB), Cicago sky blue 6B (CSB), direct blue 71 (DB-71) and thiazole yellow (TY)) effectively prevent and / or treat osteosarcoma by inhibiting the activity of PAC channels.

The invention relates to the technical field of graphene oxide composite membrane water treatment, and discloses a novel graphene oxide composite membrane with a persistent dye separation function and a preparation method thereof.The preparation method of the novel graphene oxide composite membrane with the persistent dye separation function comprises the following steps that graphene oxide and UiO-66-NH2 are prepared; the preparation method comprises the following steps: preparing a TBO (at) UiO-66-NH2 / GO composite solution containing graphene oxide, UiO-66-NH2 and a toluidine blue O solution; the TBO-coated UiO-66-NH2 / GO composite liquid is loaded to the surface of a PES film, and the novel graphene oxide composite film with the persistent dye separation function is obtained; according to the novel graphene oxide composite membrane disclosed by the invention, a'vertical support-horizontal screening 'synergistic structure is constructed, and the dye separation performance and stability of the membrane are remarkably improved.

This invention proposes a sperm staining kit based on a sequential staining strategy of tail pre-staining – rapid differentiation – enhanced nuclear staining. A gentle fixation with 4% paraformaldehyde and acetate buffer preserves the complete nucleic acid of the sperm head and the protein structure of the tail. The addition of PVP K30 and Tween-20 effectively inhibits non-specific adsorption and reduces staining background interference. Brilliant Green SF combined with sodium tartrate is used for specific staining of the sperm tail, followed by rapid differentiation to wash away non-specific staining. Nuclear staining is performed using toluidine blue O combined with sodium chloride and sodium tetraborate hydrate, specifically binding to DNA and enhancing color development stability, resulting in a blue sperm head and a light green tail with clear color contrast. This invention produces a clean staining background, accurately distinguishing the morphology of the sperm head, acrosome, and tail, significantly improving the accuracy of microscopic identification of abnormal sperm such as large-headed, small-headed, curled-tailed, broken-tailed, and short-tailed sperm. It is suitable for detecting sperm morphology and abnormality rates in breeding males.

The invention relates to the technical field of forest tree genetic breeding and plant reproduction regulation and control, and particularly discloses a black poplar and white poplar distant hybridization obstacle breakthrough method based on sodium benzoate treatment. According to the method, female flowers of black poplar are taken as a treatment object, a sodium benzoate aqueous solution with the final concentration of 5-15 mM (preferably 10 mM) is used for carrying out dipping treatment on the female flowers, and then poplar pollen pollination is carried out. After pollination, fixing, softening and aniline blue dyeing are carried out, and pollen germination and pollen tube growth conditions are observed. Experimental results show that the method can significantly promote the growth of the poplar pollen on the black poplar stigma, improve the ability of a pollen tube to penetrate through the stigma, and realize fertilization and hybrid embryo formation, thereby effectively breaking through the distant hybridization obstacle between different branches of the poplar. The method disclosed by the invention is simple and convenient to operate, low in cost and good in repeatability, provides an efficient and feasible technical means for distant hybridization breeding of poplars, and has a good popularization and application prospect.

The invention belongs to the technical field of biological probe preparation, and particularly relates to a fluorescent probe for hypochlorous acid detection and application thereof. The fluorescent probe comprises a near infrared fluorescent probe NIR-CDs and a red light fluorescent probe R-CDs, and the NIR-CDs probe is synthesized and prepared by taking toluidine blue dye as a precursor and introducing gallic acid; the R-CDs are prepared by using a nuclear fast red dye as a precursor to adjust the emission wavelength of carbon dots and introducing biuret. The fluorescent probe provided by the invention is simple and convenient in preparation method, easy to operate and stable and reliable in detection result, and compared with part of types of probes in the prior art, the probe material provided by the invention has the advantages of high sensitivity, wide application range, stable fluorescent signal and the like, so that the probe material can be used for preparing the fluorescent probe. The fluorescent probe has very important practical value and popularization and application significance in hypochlorite (ClO-) detection or related fluorescence imaging detection application.

The application discloses a kind of methods for constructing electrochemical immunosensor for detecting apolipoprotein A4, comprising: (1) nitrogen-doped graphene and polyethyleneimine are fully stirred in solvent Composite, then add bipyridine functionalized covalent organic framework material and fully stir Composite, obtain NG-PEI-COF composite material;With bipyridine functionalized covalent organic framework material as carrier, gold nanoparticles, apolipoprotein A4 antibody and toluidine blue are loaded in sequence, to obtain tracer probe;(2) on electrode NG-PEI-COF composite material, gold nanoparticles, apolipoprotein A4 and tracer probe are modified in sequence, to obtain the electrochemical immunosensor of the application.The electrochemical immunosensor constructed by the method shows excellent detection performance for apolipoprotein A4, the marker of depression, good reproducibility and stability, and shows good anti-interference performance for a variety of other markers of depression, can be accurately determined in 0.01~300 ng / mL linear range, detection limit is as low as 2.16 pg / mL, and can be applied to serum sample.

The application provides a heparin coating density and shedding rate detection method based on a package, and relates to the technical field of heparin quantitative detection. The heparin coating density and shedding rate detection method based on the package comprises the following steps: S1, drawing a heparin concentration-delta absorbance standard curve by using the toluidine blue method; S2, measuring the dynamic circulation and shedding amount of the heparin coating in the package; S3, measuring the residual amount of the heparin coating in the package; and S4, calculating the heparin coating density p and the coating shedding rate F at each time point in the package n The application can more truly simulate the fluid environment in the extracorporeal circulation process, realizes accurate measurement, can deeply study the coating stability under the dynamic scouring environment, and provides more accurate and effective evaluation basis for the long-term stability and service life of the long package.

The application discloses a method for nondestructive detection of the content of beta-1,3-glucan in Ganoderma mycelium, and relates to the technical field of biological detection, and comprises the following steps: preparing Ganoderma mycelium samples of different varieties; determining the content of beta-1,3-glucan in the Ganoderma mycelium samples of different varieties by adopting an aniline blue fluorescence method; collecting near-infrared spectrum data of the Ganoderma mycelium of different varieties; pre-processing spectrum data; screening characteristic wavelengths; establishing a prediction model; and using the model for prediction. The prediction model for the detection of the content of beta-1,3-glucan in Ganoderma mycelium based on near-infrared spectrum technology and fluorescence quantitative technology can quickly, high-throughput and nondestructively detect the content of beta-1,3-glucan, has good prediction effect, short determination time, and the advantages of simple operation, rapidness, low cost and the like.

The application discloses a red dye solution, a reagent combination and a method for Masson trichrome staining, and belongs to the field of histochemistry technology. The red dye solution comprises 0.4-1.0% of bibracine, 0.4-1.0% of temptation red and 1-2% of acetic acid, and water is used as a solvent, and the red dye solution further comprises 0.05-0.15% of orange G sodium salt; the reagent combination comprises the red dye solution and further comprises a blue dye solution, the blue dye solution comprises 2% of aniline blue, 1-2% of acetic acid, and water is used as a solvent, and the blue dye solution further comprises 0.005-0.03% of fast green, 0.0025-0.015% of brilliant green or 0.005-0.03% of bromocresol green sodium salt. The reagent combination further comprises hematoxylin dye solution, red differentiation agent and blue differentiation agent. The improved red dye solution for Masson trichrome staining provided by the application increases the affinity for muscle fiber dyeing, makes the muscle fiber dyeing more firm, and can still maintain bright red after phosphomolybdic acid differentiation. Meanwhile, the improved blue dye solution makes the collagen fiber dyeing more bright, and the color contrast between the muscle fiber and the collagen fiber is more obvious and easier to distinguish.

This application belongs to the field of biological probe preparation technology, specifically relating to a fluorescent probe for hypochlorous acid detection and its application. The fluorescent probe includes two types: near-infrared fluorescent probes (NIR-CDs) and red fluorescent probes (R-CDs). NIR-CDs are prepared by introducing gallic acid as a precursor using toluidine blue dye; R-CDs are prepared by introducing biuret to adjust the emission wavelength of the carbon dots using nucleoside red dye as a precursor. The fluorescent probe provided in this application has a simple and easy-to-operate preparation method, and the detection results are stable and reliable. Compared with some types of probes in the prior art, the probe material provided in this application has advantages such as high sensitivity, wide applicability, and stable fluorescence signal. Therefore, it is particularly suitable for detecting hypochlorous acid (ClO₂). ‑ It has significant practical value and promotional significance for detection and related fluorescence imaging detection applications.

The invention relates to the technical field of detection, in particular to a beta-1, 3-glucan determination method based on a fluorescent qualitative and quantitative technology. The method comprises the following steps: mixing a beta-1, 3-glucan detection sample solution diluted by a low-concentration sodium hydroxide solution with a high-concentration sodium hydroxide solution, carrying out a metal bath reaction, carrying out an ice bath after the metal bath reaction, adding an aniline blue working solution into the mixed solution after the ice bath, and carrying out a metal bath dark reaction, decoloring the uncombined aniline blue working solution in the reacted mixed solution, performing fluorescence detection, and comparing with a beta-1, 3-glucan standard curve based on a fluorescence detection result to obtain a detection result; the method is high in specificity and sensitivity and simple and convenient to operate, and the detection efficiency of beta-1, 3-glucan is improved.

The application provides a method for evaluating the feeding behavior of mosquito larvae and application thereof, and belongs to the technical field of insect feeding. The application provides a method for evaluating the feeding behavior of mosquito larvae, wherein after yeast dyed by aniline blue is used as feed to feed mosquito larvae, the feeding level is reflected according to the content of aniline blue in the mosquito larvae; the application also provides application of the method in screening and / or developing drugs for regulating the feeding behavior of mosquito larvae. The examples show that the method can accurately reflect the change of the feeding behavior of mosquito larvae, and has important significance for screening and / or developing drugs for regulating the feeding behavior of mosquito larvae and then inhibiting the growth and development of the mosquito larvae.

The invention discloses a staining reagent for helicobacter pylori staining and a preparation method and a staining method thereof, and belongs to the technical field of biomedicine. The dyeing reagent for helicobacter pylori dyeing is characterized by comprising eosin, methylene blue, aniline blue and methanol, the mass ratio of the eosin to the methylene blue is (0.3-0.4): 1. In addition, the invention further provides a preparation method of the dyeing reagent for helicobacter pylori dyeing and a helicobacter pylori dyeing method. The staining reagent provided by the invention can be used for rapidly and specifically staining the helicobacter pylori.

The invention discloses a fluorescent microscopic observation method for the attachment condition of hibiscus pollen on stigmas, which comprises the following steps: firstly, selecting hibiscus flowers which bloom completely, picking off pistils, and quickly cutting stigmas to obtain stigma samples; putting the column head sample into the stationary liquid, and standing for more than 24 hours; taking out the column head sample from the stationary liquid, longitudinally cutting from the top end of the column head, and averagely dividing the column head into a plurality of parts to obtain a column head tissue; the stigma tissue is soaked in a sodium hydroxide solution for standing, and the standing time does not exceed 3 hours; taking a column head sample, washing with distilled water, and dyeing with a water-soluble aniline blue solution; placing the stigma sample in a glass slide, and gently rolling the stigma sample into a slice by using a cover glass, so that the tissue is semitransparent; and placing the glass slide on which the column head sample is placed under a fluorescence microscope for observation. The method provided by the invention can effectively observe the attachment condition of the hibiscus pollen on the stigma, and provides support for evaluation of the stigma pollen attachment condition and research of hibiscus pollination biology.

The application belongs to the technical field of microorganisms, and particularly relates to a use of streptomyces in efficient decolorization and degradation of dyes; the streptomyces is MS-S2, the streptomyces MS-S2 can grow on an inorganic salt culture medium with malachite green, aniline blue, reactive blue and reactive red as the only carbon source, and has the ability of decolorization and degradation of dyes. It is verified through experiments that the streptomyces MS-S2 can quickly and efficiently decolorize and degrade malachite green, the decolorization rate is as high as 100% in a short time, and the streptomyces can adapt to a wide pH range, and the degradation product is non-toxic and non-harmful. The streptomyces can be applied to wastewater treatment in the industries of printing and dyeing, spinning, breeding and the like.

The invention provides an overdyeing kit capable of simultaneously detecting CD8 + T cells and collagen and application of the overdyeing kit, and belongs to the technical field of pathological detection. The overdyeing kit capable of simultaneously detecting the CD8 + T cells and the collagen, provided by the invention, comprises a CD8 immunohistochemical staining reagent and an improved aniline blue staining kit. According to the overdyeing kit provided by the invention, the spatial distribution of the collagen matrix and the CD8 + T cells in the tumor microenvironment can be synchronously detected by a single slice through an overdyeing strategy, the CD8 + T cells (brown) and collagenous fibers (blue-green) can be respectively and specifically marked, the dyeing contrast ratio is remarkably improved, and meanwhile, the histomorphological integrity is reserved. Compared with the traditional step-by-step staining method, the overdyeing kit provided by the invention breaks through the technical bottlenecks of high consumption of tissue samples and complex operation in multiple staining.

The invention provides a novel pseudomonas strain and application thereof in decoloration of triphenylmethane dyes, and belongs to the technical field of microorganisms. The novel pseudomonas strain RC10 provided by the invention has the capability of degrading triphenylmethane dyes such as aniline blue and can be used for decoloring the triphenylmethane dyes, the decoloring rate within 48 hours is as high as 90% or above, and the effect is remarkable.

The utility model relates to a detection swab filled with aniline blue staining fluid, which effectively solves the problems of multiple detection preparation procedures and low detection speed. Comprising a bulb, the bottom of the bulb is open, a plug is connected to the bottom of the bulb in a sealed mode, aniline blue staining fluid is contained in the bulb, a sealed glass tube is further placed in the bulb, a hydrogen peroxide solution is contained in the glass tube, a partition tube extends out of the upper end of the plug, a swab is detachably connected to the lower portion of the plug, and the partition tube and the swab are connected in the plug. After the partition tube is broken, the aniline blue staining fluid can enter the swab through the plug, and a test tube is in threaded connection below the plug; the device is simple and ingenious in structure, hydrogen peroxide and aniline blue solution are mixed after sampling, the mixed solution enters the end part of the swab to react with the sample, the sample is dyed, the detection result is judged according to the color, the detection is quick, and the use is convenient.