7 results about "ANILINE BLUE" patented technology

The invention relates to the technical field of graphene oxide composite membrane water treatment, and discloses a novel graphene oxide composite membrane with a persistent dye separation function and a preparation method thereof.The preparation method of the novel graphene oxide composite membrane with the persistent dye separation function comprises the following steps that graphene oxide and UiO-66-NH2 are prepared; the preparation method comprises the following steps: preparing a TBO (at) UiO-66-NH2 / GO composite solution containing graphene oxide, UiO-66-NH2 and a toluidine blue O solution; the TBO-coated UiO-66-NH2 / GO composite liquid is loaded to the surface of a PES film, and the novel graphene oxide composite film with the persistent dye separation function is obtained; according to the novel graphene oxide composite membrane disclosed by the invention, a'vertical support-horizontal screening 'synergistic structure is constructed, and the dye separation performance and stability of the membrane are remarkably improved.

This invention proposes a sperm staining kit based on a sequential staining strategy of tail pre-staining – rapid differentiation – enhanced nuclear staining. A gentle fixation with 4% paraformaldehyde and acetate buffer preserves the complete nucleic acid of the sperm head and the protein structure of the tail. The addition of PVP K30 and Tween-20 effectively inhibits non-specific adsorption and reduces staining background interference. Brilliant Green SF combined with sodium tartrate is used for specific staining of the sperm tail, followed by rapid differentiation to wash away non-specific staining. Nuclear staining is performed using toluidine blue O combined with sodium chloride and sodium tetraborate hydrate, specifically binding to DNA and enhancing color development stability, resulting in a blue sperm head and a light green tail with clear color contrast. This invention produces a clean staining background, accurately distinguishing the morphology of the sperm head, acrosome, and tail, significantly improving the accuracy of microscopic identification of abnormal sperm such as large-headed, small-headed, curled-tailed, broken-tailed, and short-tailed sperm. It is suitable for detecting sperm morphology and abnormality rates in breeding males.

The invention relates to the technical field of forest tree genetic breeding and plant reproduction regulation and control, and particularly discloses a black poplar and white poplar distant hybridization obstacle breakthrough method based on sodium benzoate treatment. According to the method, female flowers of black poplar are taken as a treatment object, a sodium benzoate aqueous solution with the final concentration of 5-15 mM (preferably 10 mM) is used for carrying out dipping treatment on the female flowers, and then poplar pollen pollination is carried out. After pollination, fixing, softening and aniline blue dyeing are carried out, and pollen germination and pollen tube growth conditions are observed. Experimental results show that the method can significantly promote the growth of the poplar pollen on the black poplar stigma, improve the ability of a pollen tube to penetrate through the stigma, and realize fertilization and hybrid embryo formation, thereby effectively breaking through the distant hybridization obstacle between different branches of the poplar. The method disclosed by the invention is simple and convenient to operate, low in cost and good in repeatability, provides an efficient and feasible technical means for distant hybridization breeding of poplars, and has a good popularization and application prospect.

The application discloses a kind of methods for constructing electrochemical immunosensor for detecting apolipoprotein A4, comprising: (1) nitrogen-doped graphene and polyethyleneimine are fully stirred in solvent Composite, then add bipyridine functionalized covalent organic framework material and fully stir Composite, obtain NG-PEI-COF composite material;With bipyridine functionalized covalent organic framework material as carrier, gold nanoparticles, apolipoprotein A4 antibody and toluidine blue are loaded in sequence, to obtain tracer probe;(2) on electrode NG-PEI-COF composite material, gold nanoparticles, apolipoprotein A4 and tracer probe are modified in sequence, to obtain the electrochemical immunosensor of the application.The electrochemical immunosensor constructed by the method shows excellent detection performance for apolipoprotein A4, the marker of depression, good reproducibility and stability, and shows good anti-interference performance for a variety of other markers of depression, can be accurately determined in 0.01~300 ng / mL linear range, detection limit is as low as 2.16 pg / mL, and can be applied to serum sample.

The application discloses a method for nondestructive detection of the content of beta-1,3-glucan in Ganoderma mycelium, and relates to the technical field of biological detection, and comprises the following steps: preparing Ganoderma mycelium samples of different varieties; determining the content of beta-1,3-glucan in the Ganoderma mycelium samples of different varieties by adopting an aniline blue fluorescence method; collecting near-infrared spectrum data of the Ganoderma mycelium of different varieties; pre-processing spectrum data; screening characteristic wavelengths; establishing a prediction model; and using the model for prediction. The prediction model for the detection of the content of beta-1,3-glucan in Ganoderma mycelium based on near-infrared spectrum technology and fluorescence quantitative technology can quickly, high-throughput and nondestructively detect the content of beta-1,3-glucan, has good prediction effect, short determination time, and the advantages of simple operation, rapidness, low cost and the like.

The application discloses a red dye solution, a reagent combination and a method for Masson trichrome staining, and belongs to the field of histochemistry technology. The red dye solution comprises 0.4-1.0% of bibracine, 0.4-1.0% of temptation red and 1-2% of acetic acid, and water is used as a solvent, and the red dye solution further comprises 0.05-0.15% of orange G sodium salt; the reagent combination comprises the red dye solution and further comprises a blue dye solution, the blue dye solution comprises 2% of aniline blue, 1-2% of acetic acid, and water is used as a solvent, and the blue dye solution further comprises 0.005-0.03% of fast green, 0.0025-0.015% of brilliant green or 0.005-0.03% of bromocresol green sodium salt. The reagent combination further comprises hematoxylin dye solution, red differentiation agent and blue differentiation agent. The improved red dye solution for Masson trichrome staining provided by the application increases the affinity for muscle fiber dyeing, makes the muscle fiber dyeing more firm, and can still maintain bright red after phosphomolybdic acid differentiation. Meanwhile, the improved blue dye solution makes the collagen fiber dyeing more bright, and the color contrast between the muscle fiber and the collagen fiber is more obvious and easier to distinguish.

The utility model relates to a detection swab filled with aniline blue staining fluid, which effectively solves the problems of multiple detection preparation procedures and low detection speed. Comprising a bulb, the bottom of the bulb is open, a plug is connected to the bottom of the bulb in a sealed mode, aniline blue staining fluid is contained in the bulb, a sealed glass tube is further placed in the bulb, a hydrogen peroxide solution is contained in the glass tube, a partition tube extends out of the upper end of the plug, a swab is detachably connected to the lower portion of the plug, and the partition tube and the swab are connected in the plug. After the partition tube is broken, the aniline blue staining fluid can enter the swab through the plug, and a test tube is in threaded connection below the plug; the device is simple and ingenious in structure, hydrogen peroxide and aniline blue solution are mixed after sampling, the mixed solution enters the end part of the swab to react with the sample, the sample is dyed, the detection result is judged according to the color, the detection is quick, and the use is convenient.