2 results about "ANILINE BLUE" patented technology

This invention proposes a sperm staining kit based on a sequential staining strategy of tail pre-staining – rapid differentiation – enhanced nuclear staining. A gentle fixation with 4% paraformaldehyde and acetate buffer preserves the complete nucleic acid of the sperm head and the protein structure of the tail. The addition of PVP K30 and Tween-20 effectively inhibits non-specific adsorption and reduces staining background interference. Brilliant Green SF combined with sodium tartrate is used for specific staining of the sperm tail, followed by rapid differentiation to wash away non-specific staining. Nuclear staining is performed using toluidine blue O combined with sodium chloride and sodium tetraborate hydrate, specifically binding to DNA and enhancing color development stability, resulting in a blue sperm head and a light green tail with clear color contrast. This invention produces a clean staining background, accurately distinguishing the morphology of the sperm head, acrosome, and tail, significantly improving the accuracy of microscopic identification of abnormal sperm such as large-headed, small-headed, curled-tailed, broken-tailed, and short-tailed sperm. It is suitable for detecting sperm morphology and abnormality rates in breeding males.

The application discloses a kind of methods for constructing electrochemical immunosensor for detecting apolipoprotein A4, comprising: (1) nitrogen-doped graphene and polyethyleneimine are fully stirred in solvent Composite, then add bipyridine functionalized covalent organic framework material and fully stir Composite, obtain NG-PEI-COF composite material;With bipyridine functionalized covalent organic framework material as carrier, gold nanoparticles, apolipoprotein A4 antibody and toluidine blue are loaded in sequence, to obtain tracer probe;(2) on electrode NG-PEI-COF composite material, gold nanoparticles, apolipoprotein A4 and tracer probe are modified in sequence, to obtain the electrochemical immunosensor of the application.The electrochemical immunosensor constructed by the method shows excellent detection performance for apolipoprotein A4, the marker of depression, good reproducibility and stability, and shows good anti-interference performance for a variety of other markers of depression, can be accurately determined in 0.01~300 ng / mL linear range, detection limit is as low as 2.16 pg / mL, and can be applied to serum sample.