Method for decoloring and degrading soluble azo dyes through catalysis of chloroperoxidase and oxidation of H2O2

A chloroperoxidase, azo dye technology, applied in chemical instruments and methods, oxidized water/sewage treatment, water/sewage treatment, etc., can solve the problem of low chromaticity removal rate, large fluctuation of treatment effect and inability to treat dyes and other problems, to achieve the effect of improving biodegradability, fast speed and simple operation steps

Inactive Publication Date: 2010-07-21
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently commonly used coagulation method is basically unable to deal with such water-soluble dyes, and various chemical oxidation methods have problems such as low chromaticity removal rate, large fluctuations in treatment effect, complex process and secondary pollution.

Method used

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  • Method for decoloring and degrading soluble azo dyes through catalysis of chloroperoxidase and oxidation of H2O2
  • Method for decoloring and degrading soluble azo dyes through catalysis of chloroperoxidase and oxidation of H2O2
  • Method for decoloring and degrading soluble azo dyes through catalysis of chloroperoxidase and oxidation of H2O2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Taking the decolorization and degradation of 1000 mL of a water-soluble aniline blue solution with a concentration of 10 mmol / L as an example, the raw materials used and the decolorization and degradation methods are as follows:

[0022] 1. Prepare the solution

[0023] The amount and concentration of the formulated substance is 0.1mol / L KH 2 PO 4 -K 2 HPO 4 Buffer solution, adjust its pH to 5.5 with 3mol / L hydrochloric acid, use this buffer to prepare a chloroperoxidase solution with a substance concentration of 0.1mmol / L, and use 30% H 2 O 2 The solution and distilled water are prepared according to the conventional method. The concentration of the substance is 0.2mol / L H 2 O 2 Aqueous solution.

[0024] 2. Decolorization and degradation

[0025] Add 40mL of chloroperoxidase solution with a substance concentration of 0.1mmol / L to 1000mL of a water-soluble aniline blue solution with a substance concentration of 10mmol / L, mix well, and a substance concentration of 10mmol / L water...

Embodiment 2

[0032] Taking the decolorization and degradation of 1000 mL of a water-soluble aniline blue solution with a concentration of 10 mmol / L as an example, the raw materials used and the decolorization and degradation methods are as follows:

[0033] In the decolorization and degradation step 2 of this embodiment, add 10 mL of a chloroperoxidase solution with a substance concentration of 0.1 mmol / L to 1000 mL of a water-soluble aniline blue solution with a substance concentration of 10 mmol / L, and mix well. The volume ratio of the water-soluble aniline blue solution with the substance concentration of 10mmol / L and the chloroperoxidase solution with the substance concentration of 0.1mmol / L is 1:0.01, and the substance concentration is 3mol / L H 2 SO 4 The amount of solution or substance is a NaOH solution with a concentration of 3mol / L to adjust the pH to 3, and 40mL of a substance with a concentration of 0.2mol / L is added. 2 O 2 Aqueous solution, water-soluble aniline blue solution with a...

Embodiment 3

[0035] Taking the decolorization and degradation of 1000 mL of a water-soluble aniline blue solution with a concentration of 10 mmol / L as an example, the raw materials used and the decolorization and degradation methods are as follows:

[0036] In the decolorization and degradation step 2 of this embodiment, add 60 mL of a chloroperoxidase solution with a substance concentration of 0.1 mmol / L to 1000 mL of a water-soluble aniline blue solution with a substance concentration of 10 mmol / L, and mix well. The volume ratio of the water-soluble aniline blue solution with the substance concentration of 10mmol / L and the chloroperoxidase solution with the substance concentration of 0.1mmol / L is 1:0.06, and the substance concentration is 3mol / L H 2 SO 4 The amount of the solution or substance is 3mol / L NaOH solution to adjust the pH to 3, add 80mL of the substance amount of H 0.2mol / L 2 O 2 Aqueous solution, water-soluble aniline blue solution with a substance concentration of 10mmol / L and H...

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Abstract

The invention discloses a method for decoloring and degrading soluble azo dyes through catalysis of chloroperoxidase and oxidation of H2O2, which comprises the following steps: preparing solution; decoloring and degrading; and computing the decoloring and degrading rate of the azo dyes. In the established method for decoloring and degrading soluble azo dyes orange II, bromophenol blue, methylene blue and soluble aniline blue wastewater through the catalysis of the chloroperoxidase and the oxidation of the H2O2, the H2O2 is used as an oxidant based on a bio-enzyme catalysis degradation system, a large conjugate system and azo structures and other chormophoric groups in the dyes are quickly damaged, irreversible decoloration and degradation of the dyes are realized, and the biochemical quality is improved. The method has the advantages of high speed of decoloring and degrading, high efficiency, simple operating steps, low consumption of the chloroperoxidase and the H2O2, and the like, and can be applied to treatment of industrial wastewater with soluble azo dyes.

Description

Technical field [0001] The invention belongs to the technical field of treatment of water, waste water or sewage, and specifically relates to an enzyme-catalyzed H 2 O 2 The oxidation method realizes the rapid decolorization and degradation of azo dye wastewater. Background technique [0002] my country is the largest country in textile printing and dyeing, and the textile printing and dyeing industry is also a major producer of industrial wastewater, accounting for about 35% of the entire industrial wastewater discharge. According to data, the discharge of printing and dyeing wastewater in my country is about 4 million m3 per day, and dye wastewater has become one of the key environmental pollution sources. [0003] Orange II, bromophenol blue, methine blue and water-soluble aniline blue are water-soluble azo dyes, which are widely used in the printing and dyeing industry and are one of the main components of printing and dyeing wastewater. The structure of this type of dye conta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/72C02F1/58
Inventor 蒋育澄茹雪娇胡满成李淑妮翟全国
Owner SHAANXI NORMAL UNIV
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