92 results about "Tetrabromophenol Blue" patented technology

The invention discloses an absorbing-decomposing agent of indoor harmful formaldehyde gas, its formula using active SiO2, TiO2 or Al2O3 as sorbent, peroxide as decomposer and indicating absorbing and decomposing degree of formaldehyde through the color change of the indicators such as methyl yellow, fuchsin and bromophenol blue. It adopts the mixed emulsion or powder of formaldehyde sorbent and decomposer, mixes in formaldehyde- absorbing saturated developer, and then sprays the mixture on indoor decorations or blends the mixture in indoor decorations, quickly absorbing and decomposing formaldehyde gas into harmless matters, and the decomposer after decomposing formaldehyde gas also produces harmless matters to living environment, without secondary pollution. Its formula is simple and its operation is convenient and its price is low.

A point-of-care, screening kit for use by a heath care worker to create custom test strips for screening the bodily fluids of an individual for various, medical conditions includes: (a) a plurality of reagents (12), (b) a substrate (18) configured to: i) receive one of the reagents and react with it so as to cause it to acquire a first characteristic color, and, ii) upon the addition of the individual's bodily fluid to the substrate, acquire, as a result of the formulation of each of the reagents, a second, dichotomous characteristic color when the individual has a specific one of the various, medical conditions. This kit also includes: (c) a plurality of containers (10) having indicia (26) that are reflective of the reagent within the container and which of the various medical conditions is being screened for with the use of the container and the characteristic first and second colors which are indicative of the individual having a screened for medical condition, and (d) one of the reagents being a protein reagent that includes appropriate quantities of: water, isopropyl alcohol, citric acid monohydrate, sodium citrate iribasic monohydrate, tetrabromophenol blue and tartrazine.

The invention discloses a method for treating dye waste water by enzyme production through mixed biomass fermenting. In the method, white rot fungi which can generate a lignin degrading enzyme system are used as a fermenting enzyme-production strain; a solid and liquid culture method is adopted, a cottonseed hull and/or paddy straw are/is used as a fermentation substrate, and lignin degrading enzyme is induced to obtain an optimal lignin degrading enzyme system; and finally, crude enzyme liquid is extracted, separated and prepared and can be used for decoloring treatment of waste water of commonly-used synthetic dye, such as Congo red, phenol red, bromophenol blue, crystal violet, malachite green and the like so as to obtain the optimal decoloring effect of dye sewage. The method is simple and is easy to operate. The enzyme system induced by the method disclosed by the invention has high enzymolysis efficiency, and the optimal decoloring effect can be achieved by adopting the enzyme system to treat the dye waste water.

Water-based chemical indicator inks for ethylene oxide sterilization processes and methods for its use. The chemical indicator ink contains at least one pH indicator dye selected from the group consisting of Bromocresol green, Bromophenol blue, Methyl red, Ethyl orange, and combinations thereof. The pH indicator dye undergoes an irreversible color change when exposed to ethylene oxide vapor in the presence of low-temperature steam, but when exposed to other sterilization processes either does not undergo a color change or undergoes a color change that is different than is obtained when exposed to ethylene oxide.

The invention provides a humidity indicator which is really effective in environmental protection and comprises a carrier and solution used for soaking the carrier; wherein, the solution takes 30-80% of ethanol as a solvent and contains 0.00862-0.00992% of acid-base indicator and 0.8-13.8% of hygroscopic salt. The acid-base indicator is one of bromocresol green, bromothymol blue, thymol blue, thymolphthalein, bromophenol blue, methyl red, methyl orange, methyl yellow , neutral red and phenol red or the mixture thereof. The hygroscopic salt is alkali halide or alkali-earth metal halide. The humidity indicator of the invention has wide indicating range (capable of indicating 5-90% of ambient humidity) and high sensitivity. Diverse humidity indicators different in discoloration can be manufactured by adjusting the components. The humidity indicator of the invention has evident discoloration effect, simple use, reutilization, good suitability and extensive application.

The invention provides a kit for detecting cysticercus cellulosae, swine trichinella and swine toxoplasmosis. The kit comprises sterile deionized water, polymerase chain reaction (PCR) liquid, thermus aquaticus deoxyribonucleic acid (Taq DNA) polymerase, a GoldView DNA dye, bromophenol blue sample loading buffer solution, a standard substance and a reference substance. In the detection kit provided by the invention, DNA of the cysticercus cellulosae, the swine trichinella and the swine toxoplasmosis in a specimen to be detected can be detected quickly and accurately only by one reaction, and the kit can also be used for the molecular epidemiological survey and curative effect monitoring of cysticercus cellulosae, swine trichinella and swine toxoplasmosis infection. The method has simple preparation steps and is low in cost, easy to operate, time-saving and labor-saving, a large number of reagents and consumables are saved, and the work efficiency is improved. The method is high in detection sensibility, specificity and accuracy, and the false positive rate is reduced.

The invention discloses a method for preparing cephalosporium acremonium proteome, which comprises the following steps that: cephalosporium acremonium thalli are ground into powder with liquid nitrogen, and then, total protein samples are extracted by a trichloroacetic acid (TCA)-acetone precipitation method; the obtained total protein samples are mixed with hydrated solution, hydration sampling is carried out, immobilized pH gradient (IPG) solid phase rubber strips are adopted, next, isoelectric focusing is carried out, the rubber strips are balanced after the isoelectric focusing completion and are then transferred onto sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) gel, electrophoresis is carried out, the electrophoresis is stopped when bromophenol blue indicators reach the bottom edge, and the gel is obtained; and the transmission scanning is carried out with a gel scanning instrument after the obtained gel is dyed, and the obtained images are analyzed by using software. The cephalosporin C has the important place in the cephalosporin antibiotics production, so the study on the cephalosporium acremonium proteome can be carried out, the foundation is laid for optimizing the molecular breeding and the metabolic engineering of cephalosporin producing strains, and the important significance is realized.

The invention belongs to the quick detection technology of food-borne pathogenic bacteria, and in particular relates to a detection method of loop-mediated isothermal amplification (LAMP) for salmonella. The detection method comprises the preparation of a reagent comprising a sample processing reagent, an LAMP reaction reagent, a large segment of BstDNA polymerase, agarose, bromophenol blue sampling buffer solution and ethidium bromide solution and the detection procedures of the detection an amplified product and the generation of deposition. The detection method comprises the following steps: firstly designing a specific primer; secondly extracting DNA from a sample; thirdly, establishing a reaction system. Compared with the traditional bacterial culture method, the immunity detection method and other molecular biology method adopted presently, the method has the advantages of simple operation, quickness, no special instrument reagent and specific sensitivity. The invention can be applied to the quick detection of the salmonella in foods and other samples.

The invention relates to a qualitative detection method capable of distinguishing cow milk doped in human milk. The qualitative detection method is a protein gel electrophoresis method. The electrophoresis method comprises the following steps of: putting a prepared gel plate into an electrophoresis slot, adding an electrode buffer solution, performing pre-electrophoresis under 30 to 120 volts for 15 to 60 minutes, injecting the treated test sample solution into a gel sample application hole, performing electrophoresis under 50 to 200 volts until a bromophenol blue indicator is electrophoresized to the bottom of the gel, and finally taking out the gel and fixing in a fixing solution, dyeing in a dyeing solution and decoloring in a decoloring solution so as to obtain a protein electrophoresis pattern; and comparing the sample electrophoresis pattern with a characteristic protein band in the electrophoresis pattern of pure human milk and pure cow milk prepared under the same condition so as to determine whether liquid cow milk or milk powder reconstituted milk is doped into the sample milk. In the method, only one gel electrophoresis apparatus and a conventional chemical reagent are needed, the operation is simple and the result is reliable; and the cow milk or a product of the cow milk doped into the human milk can be detected.

A thin layer detection pool for detecting serotonins and metabolites in a urine sample comprises a work electrode, a reference electrode and an auxiliary electrode, wherein the input ends of the work electrode, the reference electrode and the auxiliary electrode are connected with the output end of a liquid chromatograph; the output ends of the work electrode, the reference electrode and the auxiliary electrode are connected with the input end of an electrochemical system; the output end of the electrochemical system is connected with a computer signal acquisition end; the work electrode comprises a precious metal particle layer, a poly(bromophenol blue) layer and an electrode matrix; precious metal particles are deposited on a poly(bromophenol blue) membrane; and poly(bromophenol blue) is deposited on the electrode matrix. The thin layer detection pool manufactured in the invention can realize the detection of concentrations of the serotonins and metabolites thereof in the urine sample through simple steps, the detection linearity range can reach 1.0*10<-9>-1.0*10<-5>mol/L, and the detection resolution is 1.0*10<-10>mol/L. The detection of the concentrations of the serotonins and the metabolites thereof in the urine sample can be realized through the simple steps.

The invention belongs to a rapid detection technology of food-borne pathogens, in particular to a loop-mediated isothermal amplification (LAMP) method for detecting shigella dysenteriae DNA. The method comprises the preparation of reagent comprising sample processing reagent, LAMP reactive reagent, a large fragment of Bst DNA polyase, agarose, bromophenol blue loading buffer solution and ethidium bromide solution and the procedures of the detection of amplification products and sediment generation. The method comprises the following steps: firstly, designing a specific primer; secondly, extracting sample DNA; and thirdly, preparing a reaction system. Compared with the currently adopted traditional bacterial culture method, an immunological detection method and other molecular biology methods, the method has the advantages of simple and rapid operation, no special instrument and reagent, sensitivity and specificity and can be applied to the rapid detection of shigella dysenteriae in foods and other samples.

The invention relates to a yak molecular marker-assisted breeding technology in the technical field of animal biology and specifically relates to a yak meat tenderness candidate gene detection kit. The yak meat tenderness candidate gene PCR (polymerase chain reaction)-SSCP (single-strand conformation polymorphism) detection kit is characterized by comprising a PCR solution, DNA (deoxyribonucleic acid) standard samples of CAPN3*B and CAPN3*C, an SSCP detection reagent, deionized water, 10% of ammonium persulfate, a loading denaturation buffer solution, TEMED (N, N, N', N'-tetramethylethylenediamine) and 12% of non-denatured polyacrylamide gel, wherein Arc: Bis in the non-denatured polyacrylamide gel is 37.5: 1; the loading denaturation buffer solution comprises 98% of deionized formamide, 0.025% of bromophenol blue, 0.025% of xylene cyanol FF and 10mmol/L of EDTA (ethylenediamine tetraacetic acid) with pH value of 8.0. The yak meat tenderness candidate gene detection kit provided by the invention has the characteristics of high speed in molecular marker breeding of yak, high sensitivity, low cost and the like.

The invention relates to a method for electrophoresis determination of purity of recombinant human prourokinase for injection, wherein the method includes the following steps: (1) preparation of a test sample solution: taking the recombinant human prourokinase for injection on ice, thawing, diluting with MilliQ water, adding a non reductive sample buffer solution, placing at the temperature of 55-65 DEG C and heating for 1-5 minutes, immediately placing in an ice bath to obtain the non reductive test sample solution; (2) preparation of a Marker solution: taking a Marker solution, thawing, placing in an 80-100 DEG C water bath for 1-5 minutes, and immediately placing in an ice bath for standby application; and (3) determination method: adopting a vertical plate gel electrophoresis system, and detecting the test sample solution and the Marker solution, wherein the sample loading amount of the two solutions is 10-20 [mu]L, and the instrument initial voltage is 50-60 V; and adjusting the voltage to 100-110 V when entering a separation gel, when a bromophenol blue band runs to the lower edge of the gel, finishing the electrophoresis, after electrophoresis, dyeing and decoloring to obtain an atlas, and calculating the purity of the test sample solution according to the atlas.

The invention relates to a kit and more particularly relates to a kit for PCR (Polymerase Chain Reaction) detection of haemophilus paragallinarum. The kit comprises 20 microliters of PCR buffer solution, 25 microliters of ultrapure water, 5 microliters of Marker DL2000, 3 microliters of 15pmol/microliter upper primers, 3 microliters of 15pmol/microliter lower primers, 3 microliters of polymerase, 3 microliters of bromophenol blue, 15 microliters of TE buffer solution, 5 microliters of negative control and 5 microliters of positive control. The kit has the beneficial effects that the kit has the advantage of rapid detection of a PCR detection technology and the kit is low in construction cost; the special primers are adopted so as to meet the detection of a PCR product of a gene segment which is 0.00953ng at the minimum; the detection sensitivity is high.

The ultraviolet ray mutagenesis, selective breeding and identification method of cold tolerant Dunaliella includes: inoculating Dunaliella to culture medium; lighting and dark inducing for synchronized growth; mixing Dunaliella liquid with iodine solution or bromophenol blue solution to deactivate Dunaliella, injecting Dunaliella liquid to culture dish, mutagenesis under ultraviolet lamp before dark culturing, mixing with fresh culture liquid and painting the mixture to Dunaliella culture medium for low temperature lighting culture; inoculating single Dunaliella colony to the culture liquid and low temperature lighting culturing; sampling detection to comparing the low temperature lighting growth curves of cold tolerant Dunaliella mutant and wild prototype strain; extracting total DNA for RAPD comparison; calculating genetic similarity coefficient; extracting total protein and post-electrophoresis staining while record results; comparing protein electropherogram; and confirming the obtained cold tolerant Dunaliella mutant.

The invention belongs to a technology for simultaneously determining the content of two different effective components in a disinfectant, and concretely relates to a method for simultaneously determining the content of ethylene glycol phenyl ether and quaternary ammonium salt in the disinfectant. The determination method is characterized in that high-performance liquid chromatography is adopted to determine ethylene glycol phenyl ether in the disinfectant, ethylene glycol phenyl ether has ultraviolet absorption at 254[mu]m, reversed-phase high performance liquid chromatography (HPLC) is used to separate, qualitative analysis is carried out through using the retention time and an ultraviolet spectrogram, the peak area is used to quantify, and the mass fraction of ethylene glycol phenyl ether in the disinfectant is calculated according to a formula; and the quaternary ammonium salt and bromophenol blue and other acidic dyes form a colored salt in order to determine the content of the quaternary ammonium salt, and the colored salt can be easily extracted by a chlorine-containing organic solvent in an alkaline aqueous solution, and the combination of the colored salt is less stable than combination of sodium tetraphenylborate and the quaternary ammonium salt, so the property of the colored salt is used as the principle of the indicator sodium tetraphenylborate for titrating the quaternary ammonium salt, and the content of the quaternary ammonium salt in the disinfectant can be obtained through recording the titration amount of sodium tetraphenylborate and calculating according to a formula.

The invention discloses a method for preparing nucleic acid molecular weight standard through one-time polymerase chain reaction (PCR) to amplify all bands required by a complete marker. Two synthetical single-chain nucleotides are annealed, when double chains are formed, the two synthetical single-chain nucleotides are connected on carriers by the same tail end sequence. After conventional procedures such as transformation, etc., ascherichia coli clone is produced. Under the conditions of existing primers, dNTP, DNA polymerase and buffer fluid and according to a certain circulation conditions, the PCR amplification is carried out. The circulation amount and an amplification system are adjustable according to different requirements; the obtained material is added with appropriate amount of bromophenol blue, and the finished products are prepared. During the next production, the conservation bacteria can be cracked and used as a template (or directly adopting plasmid) to do the PCR amplification again, and the products can be fast and effectively prepared abundantly, which saves manpower, material and financial resources.

A method for mutagenizing and selectively culturing a refractory single-cell green alga includes such steps as sterilizing and cooling culture medium, inoculating alga liquid, culturing, mixing with iodine solution or bromophenol blue, deactivating, calculating alga density in alga liquid, ultraviolet mutagenizing, dark culturing, mixing the cultured alga liquid with fresh culture medium, culturing, high-temp screening, amplifying culture of living alga cells, culturing under light radiation, inoculating yellow alga, culturing, testing its mutagenized effect, measuring the long and short diameters of cell, extracting DNA, random amplifying of polymorphic DNA, calculating genetic similarity coefficient, extracting H-42 and general protein, electrophoresis, dyeing with Coomassic brilliant blue, recording result and observing the electropherogram to obtain result.

The invention discloses a subphthalocyanine/titanium dioxide nano photocatalyst and a preparation method and application thereof. The nano photocatalyst is synthesized by taking titanium dioxide as abody, adding little subphthalocyanine as a photosensitizer and utilizing a chemical modification method, and is used for improving photocatalytic performance of TiO2 under visible light. The preparation method includes: preparing titanium dioxide through a sol-gel method; preparing subphthalocyanine and the subphthalocyanine/titanium dioxide nano photocatalyst through a solvothermal method. The nano photocatalyst organically combines subphthalocyanine with titanium dioxide, visible light response range of a titanium dioxide catalyst is expanded, the titanium dioxide photocatalyst after being modified has good degradation effect on water solutions of acidic and alkaline organic dye under the visible light with wavelength greater than 400 nm, and degradation rates for acid fuchsin and bromophenol blue can reach 97% and 99% respectively. The nano photocatalyst has the advantages that the preparation method is simple, needed raw materials are few, and the photocatalyst is free of pollution, recyclable, low in cost and suitable for industrial production.

The invention provides a method for detecting the total alkaloid content of dendrobium candidum. Compared with the existing dendrobium candidum acid dye colorimetric method, the method first uses sanguinarine as a standard product to replace dendrobine so that a sample to be tested has the maximum absorption wavelength (410nm) the same to that of a complex produced by the reaction of the standardproduct and an acid dye. Preferably, bromophenol blue is used as the acid dye so that the background absorption value is reduced. The novel method has the advantages of high accuracy, good stability and easy reproduction. The method provides the accurate and effective detection method for studying the accumulation rule of total alkaloids of dendrobium candidum and the quality control of the dendrobium candidum total alkaloid preparation process.

The invention provides a separation identification and phosphorus solubilizing capacity measurement method for improved phosphate solubilizing bacteria. The method comprises the following step: getting a soil sample, performing preliminary screening and secondary screening on the oil sample, so as to screen out phosphate solubilizing bacteria, and measuring the phosphorus solubilizing capacity, wherein the diameter of a phosphorus solubilizing ring and phosphorus content in a culture solution comprehensively reflect the phosphorus solubilizing capacity of strains. According to the invention, a bromophenol blue indicator is used as a selection marker, through the addition of bromophenol blue used as the indicator for the preliminary screening of the phosphate solubilizing bacteria, and observation of the phosphorus solubilizing ring and secondary screening, target strains can be screened out effectively, rapidly and visually, in addition, through the measurement of phosphorus content in the liquid culture solution, quantification to the decomposition capacity of the phosphate solubilizing bacteria can be achieved.

The invention discloses sensitive test paper for measuring ammonia in air as well as preparation and determination methods thereof, belonging to the field of environmental monitoring. The sensitive test paper is loaded with bromophenol blue and a cationic surfactant, and the mass ratio of bromophenol blue (g) to the cationic surfactant (mg) is equal to (0.06-0.4):(0.1-0.5). The preparation methodcomprises the following steps: immersing filter paper or chromatographic filter paper in soaking liquid to obtain immersed test paper, then drying, and sealing with plastic at 110-150 DEG C, so as toobtain the sensitive test paper for measuring ammonia in air. One end of the sensitive test paper is cut open, so that ammonia in air diffuses into the test paper and reacts with the bromophenol blueand the cationic surfactant supported on the test paper to form a blue complex, and the rising height of the blue complex is measured to obtain the content of ammonia in air. The test paper has the advantages of accurate, fast, intuitive and convenient use, can be used to quantitatively detect ammonia in air on site, and can meet the field test requirements of industrial waste gas and workplace air.

The invention discloses a wetness indicating adhesive and a preparation technology thereof, and the wetness indicating adhesive is used for nursing products such as paper diapers, etc.. The wetness indicating adhesive comprises, by weight, 8-10% of microcrystalline wax, 9-10% of a benzoate plasticizer, 30-35% of hydrogenated rosin, 3-5% of stearic acid, 10-12% of glycerin monostearate, 20-30% of polyvinylpyrrolidone, 8-15% of polymerized rosin, 1-2% of an antioxidant, 0.1-1% of bromophenol blue, 0.1-1% of bromocresol green and 0.1-1% of a yellow dye. The wetness indicating adhesive is prepared by steps of melting, stirring, discharging, packaging and the like. The wetness indicating adhesive has advantages of strong coloration effect, gradient coloration, coloration stability, safety and reliability.

The invention discloses a quick detecting method of sibutramine hydrochloride. The method sequentially comprises the following steps of: (1) extracting sibutramine hydrochloride from a sample through an organic solvent; (2) extracting and purifying the sibutramine hydrochloride through an inorganic acid solution; and (3) adding bromophenol blue as an indicator, and judging the result through chromogenic reaction. The invention further provides a special detecting kit of the method. The kit comprises one or a mixed solution of methanol, ethanol, n-butyl alcohol, dichloromethane and acetonitrile, the inorganic acid solution, and the bromophenol blue solution. Compared with the prior art, the method disclosed by the invention is low in analysis cost, free of special equipment, strong in specificity, high in sensitivity, and simple, convenient and quick to operate, thereby being suitable for onsite inspection. The quick detecting kit of sibutramine hydrochloride prepared by the method can meet the demands of related food supervision and inspection in extensive rural areas.