31 results about "Dichlorofluorescein" patented technology

Autonomous detection of damage in a polymer coating is described by utilizing microcapsules in a polymer coating having free and / or residual amines. The microcapsules contain a color indicator, such as 2′,7′-dichlorofluorescein (DCF), bromophenol blue (BPB) or fluorescamine, which is reactive with the free and / or residual amines present in the polymer coating. For coatings without the presence of free and / or residual amines, a color indicator microcapsule can be combined with a second type of microcapsule filled with a base. When sufficient damage is inflicted to the coating, the microcapsules in and / or around an area of the damage will rupture, and the color indicator will react with the free and / or residual amines or the base to autonomically indicate the area in which the coating has been damaged.

The invention discloses a method for measuring the active oxygen level in phanerochaete chrysosporium in treated wastewater. The method comprises the following steps: washing a phanerochaete chrysosporium bacterium ball in the treated wastewater, adding a liquid culture medium for further culture, further adding 2',7'-dichloro dihydro fluorescein sodium diacetate into the liquid culture medium after culture to obtain a mixture solution, carrying out incubation on the mixture solution, filtering, then performing ultrasonic crushing and centrifuging on the bacterium ball obtained through filtration, extracting a supernate, and finally measuring the fluorescence intensity of the oxidized dichlorofluorescein in the supernate. The method has the advantages that the operation condition is simple, the application is easy and the active oxygen level in the phanerochaete chrysosporium in the treated wastewater can be accurately and visibly measured.

The invention provides a polymer-based fluorescent probe, which comprises an amphiphilic block copolymer and a thiol-responsive group and / or a dichlorofluorescein group grafted on the amphiphilic block copolymer. Compared with the prior art, the present invention uses naphthalimides as sulfhydryl-responsive fluorescent molecules, which have a relatively large rigid coplanar structure and a conjugated system. There is a photoinduced electron transfer mechanism in this structure. When naphthalimides After Michael addition with sulfhydryl groups, fluorescence is produced due to the obstruction of PET; thioether-esterified dichlorofluorescein molecules are used as acid-responsive fluorescent molecules, which can be induced by H + The ester bond in its structure is hydrolyzed to make it have a conjugated structure, thereby generating fluorescence; and the present invention grafts the above-mentioned fluorescent molecules on the amphiphilic block copolymer as a polymer-based fluorescent probe with a highly sensitive mercapto group And / or the detection function of acidity, so as to realize the fluorescent tracking of the path of micelles entering the living body.

The invention belongs to the technical field of plant stress and physiologic response, and particularly relates to the labeling method for active oxygen generation, generation positions and relative content under biotic stress and abiotic stress during the growth and development process of wheat. The labeling method comprises the following steps of: preparing a label load buffer solution, preparing a material tissue block, rinsing the tissue block with the load buffer solution, placing the tissue block in a dark place at 25 DEG C, exciting the tissue block by using a laser excitation device, observing the tissue block and taking a photo. Compared with the traditional labeling method of DAB (Dimethylaminoazobenzene), NBT (Nitroblue Tetrazolium) and other active oxygen species, the labeling method saves time and avoids the distortion of active oxygen content and positions of a sample during long-time labeling load period. When the active oxygen is labeled by DCFH-DA (Dichlorofluorescein Diacetate), the material is fixed, and the accuracy of active oxygen labeling is ensured. The used device has simple requirements and is easy to operate.

The invention discloses a metal-organic framework material for the difunctionalization reaction of styrene catalyzed by visible light, its preparation method and application. The molecular formula of the material is C 30 h 17 Cl 2 N 2 o 5.5 co 0.5 , the crystal system is monoclinic, the space group is C2 / c, and the unit cell parameters are: α=90.00°, β=104.889(2)°, γ=90.00°, and the number of molecules in the unit cell is 8; the material The general chemical formula is Co 0.5 (DCF)(bpy)(H 2 O) 0.5 , wherein, DCF is 2',7'-dichlorofluorescein, and bpy is 4,4'-bipyridine. The material provided by the invention has the high-efficiency performance of catalyzing the difunctionalization reaction of styrene under visible light conditions to synthesize 1,3-oxathiolane-2-imino. The preparation process of the invention is simple, the catalytic efficiency is high, and the cycle Good stability and easy large-scale preparation.

The invention belongs to the technical field of total chlorine content determination of eye wash products in the pharmaceutical industry, and particularly relates to a method for determining the total chlorine content in a balanced salt solution for perfusion, which specifically comprises the following steps: precisely measuring 10 ml of balanced salt solution for perfusion, adding 80 ml of water and 0.1 ml of dilute nitric acid, adding 5 ml of dextrin solution and 8 drops of dichlorofluorescein indicating solution, and titrating with 0.1 mol / L silver nitrate titrating solution, thus the color of the solution finally becomes light pink. The method disclosed by the invention can be successfully used for determining the total chlorine content of the balanced salt solution for perfusion, is good through methodology verification, meets the determination requirements of the total chlorine content of the balanced salt solution for perfusion, realizes accurate control of the quality of the balanced salt solution for perfusion, and provides a basis for product quality and safety.