30 results about "Dichlorofluorescein" patented technology

The invention discloses a method for detecting content of active oxygen in cells. The method has the advantage that a DCF (dichlorofluorescein) fluorescence signal value of a single live cell corresponding to pollutants exposed under different concentrations can be obtained; a detection result is accurate, and the problem of inaccurate measuring of final fluorescence intensity due to change of cell number caused by exposing of the pollutants is solved; the corresponding fluorescence signal value of single cell under a certain concentration can be truly reflected, and the injury to cells by pollutant exposing can be truly and accurately reflected, so as to lay a solid foundation for the application of an in-vitro cell model into the toxicity evaluation of the pollutants.

The invention belongs to the field of analytical chemistry and particularly relates to a reagent for determining sodium chloride content in urine, a test paper strip thereof, a preparation method thereof and a purpose thereof. The reagent is composed of a reagent A and a reagent B; the reagent A is composed of 1-60 weight parts of silver nitrate and 1 volume part of water; the reagent B contains 0.5-20 weight parts of 2,7-dichlorofluorescein and 1 volume part of organic solvent selected from at least one of alcohol, phenol, ether, acid, amide and ketone of C1-C10. The reagent B further contains 0.5-50 weight parts of surface active agents and 1-70 weight parts of sulfosalicylic acid, and the relation between the weight part and the volume part is g/L. The test paper strip for determining sodium chloride content in urine is simple and convenient to operate, low in cost, favorable for industrial production and capable of quickly and conveniently determining sodium chloride content in urine, and thus can judge whether the salt intake is excessive.

The invention discloses a method for measuring the active oxygen level in phanerochaete chrysosporium in treated wastewater. The method comprises the following steps: washing a phanerochaete chrysosporium bacterium ball in the treated wastewater, adding a liquid culture medium for further culture, further adding 2',7'-dichloro dihydro fluorescein sodium diacetate into the liquid culture medium after culture to obtain a mixture solution, carrying out incubation on the mixture solution, filtering, then performing ultrasonic crushing and centrifuging on the bacterium ball obtained through filtration, extracting a supernate, and finally measuring the fluorescence intensity of the oxidized dichlorofluorescein in the supernate. The method has the advantages that the operation condition is simple, the application is easy and the active oxygen level in the phanerochaete chrysosporium in the treated wastewater can be accurately and visibly measured.

The invention relates to fluorescent ink. The fluorescent ink contains a fluorescent agent and can produce a fluorescent effect when encountering ultraviolet rays (sunlight, fluorescent lamps and mercury lamps are more frequent) and emit white light, so that the color looks like the color has a glare fluorescent feeling. The formula comprises the following components: 5-12% of dichlorofluorescein, 5-7% of sodium hydroxide, 3-5% of sodium carbonate, 2% of phosphoric acid, 2% of balance modifier, 5-8% of ethanol, 3% of fluorescent effect, not less than 70% of distilled water and 1% of additives.

The invention discloses a preparation method of a bromofluorescein type cosmetic dyestuff. Specifically, the dyestuff disclosed by the invention is prepared from 4',5'-dibromofluorescein (C.I.45370: 1) , 2',4',5',7'-tetrabromofluorescein (C.I.45380: 2), 2',4',5',7'-tetrabromo-4,7-dichlorofluorescein (C.I.45405: 1) and 2',4',5',7'-tetrabromo-4,5,6,7-tetrabromofluorescein (C.I.45410: 1). The preparation method comprises the following steps: (I) putting bromine into liquid alkali and a water solution of sodium hypochlorite at low temperature to prepare a sodium hypobromite solution; (II) dissolving fluorescein or corresponding chlorofluorescein or partially dissolving the fluorescein or the corresponding chlorofluorescein into an acid-containing solvent; dropwise adding the sodium hypobromitesolution at room temperature and carrying out bromination; (III) after reaching a bromination finishing point, adding a proper amount of a reducing agent to eliminate hypobromous acid if residual hypobromous acid exists; after removing a solvent, washing an obtained crude product with diluted hydrochloric acid and desalting; drying to obtain a target product.

The invention provides preparation technology of allochroic silica gel and allochroic silica gel utensils. The allochroic silica gel comprises following raw materials: silica gel raw gel with a hardness of 40 DEG, coloring agents, and an allochroic powder; the allochroic powder comprises polyoxymethylene melamine, styrene maleic anhydride polymer, a surface active thickening agent, and dichloro fluorescein; and the ratio of the coloring agents to the allochroic powder is controlled to be 1:0.5-1. The allochroic silicagel is capable of displaying the color of doped coloring agents of different colors at different temperatures through the fluorescence effect of the allochroic powder with heat sensitivity; especially, color change can be realized in a very short time period at critical temperature values.

The present invention provides a method of screening for a compound that enhances or inhibits the transport activity of OATP1B1, using dichlorofluorescein. The present invention also provides a use of dichlorofluorescein in measurement of the expression level of OATP1B1. The present invention further provides a method for measuring the expression level of OATP1B1 in test cells, using dichlorofluorescein. The present invention further provides a use of a kit including dichlorofluorescein and positive cells expressing OATP1B1 in measurement of the expression level of OATP1B1 in test cells.

The invention discloses a visible light catalyzed styrene bifunctionalization reaction metal organic framework material as well as a preparation method and application thereof. The molecular formula of the material is C30H17Cl2N2O5.5Co0.5, the crystal system is monoclinic, the space group is C2/c, and the cell parameters are as follows: alpha = 90.00 degrees, beta = 104.889 (2) degrees, gamma = 90.00 degrees and the number of molecules in cells is 8. The general chemical formula of the material is Co0.5(DCF)(bpy)(H2O)0.5, wherein the DCF is 2',7'-dichlorofluorescein, and the bpy is 4,4'-dipyridyl. The material provided by the invention has high-efficiency performance of catalyzing styrene bifunctionalization reaction to synthesize 1,3-oxa-thiacyclopentane-2-imino under the condition of visible light, and is simple in preparation process, high in catalytic efficiency, good in cycling stability and easy for large-scale preparation.

The invention discloses an antitumor medicine screening kit and a using method thereof, and belongs to the technical field of biomedicine. The kit comprises caenorhabditis elegans, a nematode culture medium, escherichia coli and dye. An objective of screening effective antitumor medicines rapidly, efficiently and accurately with simple operation and a high throughout is achieved by utilizing different dyeing effects of the dye that is 2',7'-dichlorofluorescein diacetate on mutants and wild type caenorhabditis elegans, thus saving time and the cost for personnel researching and developing antitumor medicines.

The invention relates to a protein negative staining detection technology, in particular to 2',7'-dichlorofluorescein and application of a derivative thereof in protein negative staining detection. The invention further provides a method for performing protein negative staining detection by applying the 2',7'-dichlorofluorescein. The method comprises the steps that direct dyeing is performed after protein gel electrophoresis is finished; then, developing is performed. The 2',7'-dichlorofluorescein has the advantages of being high in sensitivity, easy and rapid to operate, good in reproducibility, good in linear relation, good in mass spectrum compatibility, safe to use, low in cost and the like, an can be well applicable to research on high-throughput proteomics.

The invention relates to a hydrogenated dichlorofluorescein diacetamide derivative as well as a preparation method and application thereof, and belongs to the technical field of heme detection. The hydrogenated dichlorofluorescein diacetamide derivative disclosed by the invention can be used as a fluorescence reporter molecule for heme detection, and the detection method disclosed by the inventioncan be used for conveniently and quickly detecting heme; according to the invention, three peroxides H2O2, TBHP and ART are respectively mixed with DCFHA to prepare a detection reagent; the three systems can be used for rapid detection of heme, the detection limit can reach the picomole level, the DCFHA/H2O2 detection method can successfully distinguish bloodstains and coffee stains, and fluorescence can be seen with naked eyes under ultraviolet light; the DCFHA/ART is doped into the filter paper to prepare the detection test paper, and the detection test paper has good effects in heme detection and blood determination, can be used as a portable device, and is used in criminal investigation and other practical applications.

The invention discloses a method for measuring the total chlorine content in a compound preparation containing sodium citrate, which comprises the following steps: 1, precisely measuring 10ml of the compound preparation, adding 0-3ml of 0.1 mol/L calcium nitrate solution, and adding 0-0.3 ml of dilute nitric acid; 2, adding 5ml of 2% dextrin solution and 8 drops of dichlorofluorescein indicating liquid; 3, titrating with a silver nitrate titration solution (0.1 mol/L); as a preferable technical scheme of the invention, each 1ml of silver nitrate titration solution (0.1 mol/L) is equivalent to 3.545 mg of chlorine; the method has the beneficial effects that 10 ml of the compound preparation is precisely measured, 5 ml of a 2% dextrin solution and 8 drops of the dichlorofluorescein indicator solution are added, titration is performed by using the silver nitrate titration solution (0.1 mol/L), and on the basis that 1 ml of the silver nitrate titration solution (0.1 mol/L) is equivalent to 3.545 mg of chlorine], 1 ml of the 0.1 mol/L calcium nitrate solution and 0.1 ml of dilute nitric acid are added, so that the influence of sodium citrate in the compound preparation containing sodium citrate on the total chlorine content determination can be eliminated, therefore, the accuracy of total chlorine content determination is ensured.

The invention belongs to the technical field of biomedicine, and in particular relates to an antioxidant drug screening kit and a use method thereof. The antioxidant drug screening kit provided by the present invention includes Caenorhabditis elegans, nematode culture medium, Escherichia coli OP50, 2', 7' dichlorofluorescein ethylene glycol, and can be used for high-throughput screening of antioxidant drugs. The invention is easy to operate and fast in detection, and is suitable for large-scale popularization in the field of screening antioxidant drugs.

The invention discloses a preparation method of an aniline para-trifluoromethylated derivative. The method comprises the following steps: sequentially adding an aniline derivative, 4, 5-dichlorofluorescein, potassium persulfate and sodium trifluoromethanesulfinate into a glass reaction tube, and reacting at room temperature (23-25 DEG C) under 40W blue LED (light-emitting diode) by taking dimethyl sulfoxide as a solvent to obtain the aniline para-trifluoromethylated derivative. According to the invention, the aniline derivative is used as an initiator, and the raw materials are easily available and various; products obtained by the method disclosed by the invention have various types and can be directly applied to modification of drug molecules; and meanwhile, the synthesis route is safe and easy to implement, the cost is low, the reaction operation and post-treatment process are simple, the selectivity is good, the side reaction is few, and the amplification reaction can be carried out.

The invention provides a method for quantitatively measuring the content of in-vivo/in-vitro superoxide radicals generated by environmental stress, which comprises the following steps: S1, preparing a test solution: taking a sample to be detected, and preparing the test solution; step S2, preparation of a reference substance solution: taking a standard substance 2, 7-dichlorofluorescein, and dissolving in water to prepare the reference substance solution; s3, detecting the test solution and the reference solution by adopting a high performance liquid chromatograph-fluorescence detector; and S4, drawing a standard curve by taking the peak area of the reference substance solution as a vertical coordinate and the concentration of the reference substance solution as a horizontal coordinate, and calculating the content of 2, 7-dichlorofluorescein in the test solution according to the standard curve so as to obtain the content of superoxide radicals. By adopting the technical scheme of the invention, the ROS can be quantitatively measured, the interference of external environmental factors is reduced, different batches have comparability, and the method is simple and reliable.