Method for Determination of Active Oxygen Level in Phanerochaete chrysosporium After Treating Wastewater

A technology for treating Phanerochaete protothecoides and wastewater, which is applied in the fields of wastewater treatment and microbial application, can solve the problems of high background value, complicated instrument operation and high detection cost, and achieves the effects of improving the crushing efficiency, being easy to expand the culture and having a simple preparation process.
CN103808703BInactive Publication Date: 2016-06-01HUNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUNAN UNIV
Publication Date
2016-06-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for measuring the active oxygen level in phanerochaete chrysosporium in treated wastewater. The method comprises the following steps: washing a phanerochaete chrysosporium bacterium ball in the treated wastewater, adding a liquid culture medium for further culture, further adding 2',7'-dichloro dihydro fluorescein sodium diacetate into the liquid culture medium after culture to obtain a mixture solution, carrying out incubation on the mixture solution, filtering, then performing ultrasonic crushing and centrifuging on the bacterium ball obtained through filtration, extracting a supernate, and finally measuring the fluorescence intensity of the oxidized dichlorofluorescein in the supernate. The method has the advantages that the operation condition is simple, the application is easy and the active oxygen level in the phanerochaete chrysosporium in the treated wastewater can be accurately and visibly measured.
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Description

technical field

[0001] The invention relates to the field of microorganism application and waste water treatment, in particular to a method for measuring the active oxygen level in Phanerochaete chrysosporium cells after waste water is treated. Background technique

[0002] At present, the harm of heavy metals and organic pollutants to the environment has become a global problem. In the current field of wastewater treatment, the existing technology has been able to use Phanerochaete chrysosporium to remove heavy metals and organic matter in wastewater. Receptivity also deserves our attention.

[0003] Existing detection methods for reactive oxygen species are mainly used in the detection of reactive oxygen species in higher organisms such as animals and plants. The detection method used is basically flow cytometry, but the price of flow cytometry is high, the detection cost is high, and the operation of the instrument is complicated. , and it is not suitable for batch test...

Claims

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