73 results about "Dielectrophoretic force" patented technology

An apparatus for performing laser cutting and collection of a target micro-region of a tissue section on a blade. The apparatus comprises a carrier stage, a blade, a laser cutting system, a control module and a collector, wherein the laser cutting system comprises a laser device, a beam expander, a galvanometer scanner and an objective lens; and by means of adjusting a scanning angle θ of the galvanometer scanner and combining same with the objective lens, a scanned laser is focused to realize the cutting of a target micro-region on a tissue section and the ejection of the target micro-region. Laser microdissection is combined with mechanical sectioning, and non-contact collection of a specific micro-region of a tissue section placed on an inclined blade is achieved under the action of laser edge ejection combined with a dielectrophoretic force, such that the integrity of the morphology of a sample throughout an acquisition process is ensured, three-dimensional positional information of a target cell or cell population is accurately reserved, the cutting efficiency is improved, and non-contact cutting can also be realized to prevent the sample from being contaminated.

The invention discloses an NKT cell culture optimization method, and belongs to the technical field of cell culture.The method comprises the steps that firstly, PBMC is sorted through a spiral micro-fluidic chip integrated with CD3 / CD56 double-labeled magnetic beads, and CD3 + CD56 + cells with the purity larger than or equal to 95% are obtained through the synergistic effect of dielectrophoretic force and a magnetic field; then, in a GIBCO culture solution added with the IL-2 / IL-15 / alpha-GalCer sustained release microspheres, the pH, dissolved oxygen and lactic acid concentration are accurately regulated and controlled through a real-time monitoring system, and temperature-oxygen partial pressure gradient culture is adopted; and finally, three times of centrifugation are combined with NKT4 solution treatment, and the cells are collected in cooperation with a gradient cooling procedure, so that the cell activity maintenance rate is greater than or equal to 95%. Compared with the traditional technology, the method has the advantages that the NKT cell sorting purity and activity are remarkably improved, the culture environment is regulated and optimized through dynamic metabolism, and accurate control over the culture process is achieved.

A method of producing a battery electrode includes applying an electrode material onto a surface of a current collector, the electrode material comprising a binder material having binder particles, initiating a drying process to dry the electrode material, and applying a non-uniform electric field to the electrode material during the drying process. The non-uniform electric field defines a magnitude gradient to generate dielectrophoretic forces on the binder particles, thereby moving the binder particles.

The invention discloses an electrically-driven integrated liquid drop microfluidic device, relates to the technical field of microfluidics, and is used for solving the technical problems that most of the existing control modes are limited to plane control and cannot adapt to unstructured working environments in a deep cavity and a biochemical analyzer. The electrically-driven integrated liquid drop microfluidic device comprises a supporting plate, a liquid collecting plate, a detection platform, a displacement mechanism and a liquid transferring mechanism, the supporting plate is provided with a first surface and a second surface which are oppositely arranged; the supporting plate is arranged on the fixed platform through the first surface; the liquid collecting plate is arranged on the supporting plate through the second surface; the detection platform is arranged at any end of the liquid collection plate; the displacement mechanism is arranged to move in the horizontal and / or vertical direction relative to the liquid collecting plate; the pipetting mechanism is arranged on the displacement mechanism and is used for moving the liquid drops or the liquid marbles from the liquid collection area to the detection platform through dielectrophoretic force.

This invention provides a microplastic particle screening and separation device and method based on photoinduced dielectrophoresis. The device comprises a photoinduced dielectrophoresis chip consisting of a first illumination region, a second illumination region, a controllable barrier block, and a microchannel layer. By applying light to different positions on the photoinduced dielectrophoresis chip, the conductivity at the illumination position changes, thereby generating a non-uniform electric field. Due to the different radii of the microplastic particles, the magnitude of the photoinduced dielectrophoretic force varies. Larger-radius microplastic particles experience a greater dielectrophoretic force and cannot pass through the illumination region, while smaller-radius microplastic particles experience a smaller dielectrophoretic force and can flow to the sample outlet. By changing the position of the controllable barrier block, the separation of microplastic particles of three different radii can be achieved. The microplastic particle screening and separation device and method based on photoinduced dielectrophoresis described in this invention can achieve the separation and screening of target microplastics of different sizes using contactless photoinduced dielectrophoresis force.

The application discloses a kind of microscale microplastic migration occurrence multi-physical field coupling simulation method, belong to ecological environment field.The method aims at solving the problem that existing microplastic migration model ignores interface adsorption process, is difficult to couple the action of multiple physical fields and is not suitable for the problem of insufficient adaptability to heterogeneous environment.Its main points are: in the Lagrangian framework, by constructing seepage field, and comprehensively calculating the stokes drag, dielectrophoresis force and Brownian force suffered by microplastic particles to track its movement, while explicitly introducing the adsorption and desorption between particles and pore wall, to simulate the migration trajectory and occurrence state of microplastic in heterogeneous porous medium.The application provides a high-precision simulation tool for revealing the environmental fate and health risk of microplastics in groundwater systems.

The invention discloses a method for quickly removing bubbles in a porous medium based on electric field regulation and control, which comprises the following steps: arranging an electrode in a mold, and applying a non-uniform electric field to a porous medium flow field containing bubbles through the electrode in a resin impregnation process; based on a non-uniform electric field, dielectrophoretic force is induced to be generated at an interface of fluid-bubble-porous medium; through dielectrophoretic force, the bubble form is regulated and controlled, and the bubbles are driven to accelerate directional migration, so that the bubbles escape from the porous medium. According to the method, the limitation that a traditional bubble removal method mainly depends on pressure gradient and capillary force passive gaming is overcome, efficient, accurate and active regulation and control and rapid removal of the tiny bubbles in the porous medium are achieved, and the porosity of the composite material is effectively reduced.

The invention discloses a photoelectric tweezer chip and a manufacturing method thereof, according to the photoelectric tweezer chip, a photoconductive unit is arranged on a substrate, when a light beam irradiates the photoconductive unit, dielectrophoretic force can be excited and generated, and effective control over cells can be achieved. And the middle layer formed by the PEG material layer is arranged above the photoconductive unit, and the PEG material can protect the photoconductive unit and prevent adhesion of micro-objects. A plurality of nano columns distributed at intervals are arranged above the middle layer, and when a micro object is in contact with the nano columns, cell adhesion can be effectively avoided due to a small contact area; the dielectric constant difference between the nanorods and the solution can also form a concentrated electric field, repulsive force can be generated on micro-objects, and the cells are further prevented from being adhered to the photoelectric tweezers chip.

The application provides a microfluidic chip and a biological particle separation device based on the microfluidic chip, which comprises a main flow channel, a plurality of bypass flow channels and an electrode flow channel. The electrode flow channel is arranged on both sides of the side wall of the main flow channel and forms a plurality of electrode zones along the extension direction of the main flow channel, so as to provide a non-uniform electric field for the main flow channel; the plurality of bypass flow channels are sequentially communicated with the main flow channel along the extension direction of the main flow channel, so that the electrode zones and the bypass flow channels are alternately and spacedly arranged along the extension direction of the main flow channel. Thus, after the sample liquid is injected into the main flow channel, different target biological particles in the sample liquid can produce different lateral deviations under the dielectrophoresis force of one or more electrode zones, so that the target biological particles can flow out from the liquid outlet of the corresponding bypass flow channel or the main flow channel, and the efficient separation of biological particles of multiple sizes and types is realized.

With this imaging method, dielectric particles in a fluid chip are imaged by an imaging unit. The fluid chip has an electrode that causes dielectrophoretic force to act on the dielectric particles contained in a fluid injected into the fluid chip. The imaging method includes: a step for causing the imaging unit to focus on the electrode; and a step for changing a position for focusing by the imaging unit to a position separated from a position of the electrode by a predetermined distance in a first direction. The first direction follows the optical axis of the imaging unit and is indicative of a direction from the inner bottom surface to the inner top surface of the fluid chip.

A dielectrophoretic detection device including a chip, with a flow channel having at least one inlet and one outlet, and at least a detection area configured to detect analytes trapped on functionalised beads flowing within the flow channel, first and second electrode assemblies shaped as rows of parallel pillars extending a the height of the flow channel, and configured to generate under electric tension an electric field to form an electrical barrier, and preventing the beads to cross the barrier and drawing the beads to the detection area by dielectrophoretic forces where they are clustered and concentrated. The device may be provided with multiple rows of parallel pillars of electrode assemblies extending over the height of the flow channel, forming multiple concentration lines. The flow channel may be provided with further rows of parallel pillars of electrode assemblies crossing the flow channel in a transverse direction, forming further incubation lines.

The invention provides a micro-fluidic chip and an intelligent particle sorting system and equipment. The micro-fluidic chip comprises a channel layer and a substrate layer which are arranged from top to bottom, the channel layer comprises an inlet flow channel, a shunting flow channel, a sorting flow channel, an outlet flow channel and an electrode flow channel; one end of the inlet runner is connected with the sample inlet and the sheath fluid inlet, and the other end of the inlet runner is connected with the shunt runner; a sample and sheath fluid flow through the inlet flow channel, flow into the shunting flow channel and then flow through the sorting channel for sample particle sorting, and the sorted sample particles flow out through the outlet flow channel; the electrode flow channel enables the side wall of the sorting flow channel to form a side wall electrode, and the side wall electrode enables the sample to deflect due to dielectrophoretic force when flowing through the sorting channel so as to sort sample particles. According to the invention, the particles can be sorted efficiently and intelligently in a high-throughput manner.

The invention relates to the technical field of electric appliance engineering, in particular to a metal particle trap structure based on electrostatic induction. Through the synergistic effect of the electrostatic induction electrode and the high-adsorption specific surface structure, a metal particle active capture trap mechanism is constructed. A high-gradient electric field generated by the induction electrode guides polarized metal particles in the environment to migrate to the trap groove; the trap groove dissipates kinetic energy of collision metal particles through a special inclined plane geometric structure and a surface coating, so that the particle capturing probability is improved; and meanwhile, the metal particles are finally deposited at the bottom of the trap in the migration process by combining the effects of structural diversion, dielectrophoretic force induction and gravity settling. The above various synergistic effects significantly improve the global capture efficiency of particles of different materials, sizes and motion states, and the method is suitable for a direct current GIL / GIS environment with complex electric field distribution.

A channel chip (11) includes a substrate (21), a liquid receiver (FW), a pair of first electrodes (33a), (33b), and a pair of second electrodes (43a), (43b). A sample liquid or a reference liquid can be injected into the liquid receiver (FW). When a control voltage is applied, the pair of first electrodes (33a), (33b) can cause a dielectrophoretic force to act on dielectric particles (P1) contained in the sample liquid injected into the liquid receiver (FW). The pair of second electrodes (43a), (43b) are disposed at positions different from positions of the pair of first electrodes (33a), (33b). The control voltage applied between the pair of first electrodes (33a), (33b) are corrected based on the impedance between the pair of second electrodes (43a), (43b) measured through the reference liquid injected into the liquid receiver (FW).

The present invention provides a multi-stage sorting device and method for marine microbial particles based on a U-shaped microfluidic chip. The device includes a first sorting zone, a U-shaped microchannel, and a second sorting zone. The first sorting zone includes a sample inlet, a ratchet structure, an oil inlet, a first microfluidic straight channel, a sample outlet, and a positive power supply insertion port. The second sorting zone includes two sample outlets, a negative power supply insertion port, and a second microfluidic straight channel. The oil and sample particles form microdroplets at the connection between the oil channel and the first microfluidic straight channel. Due to the different dielectrophoretic forces required for particle separation, the microdroplets have different sizes. Both the first and second sorting zones use direct current dielectrophoresis. Two external electrodes act in the microchannel to generate a non-uniform electric field at the microdroplets. Marine microbial particles sorted in the sorting zones enter different sample outlets based on their size and the magnitude of the dielectrophoretic force, thereby achieving multi-stage sorting of marine microbial particles.

The invention discloses a cell manipulation observation device and a cell manipulation observation method.According to the cell manipulation observation device, an initial light beam is emitted through a light source module, the initial light beam is split through a light splitting module to form a first light beam and a second light beam, and at the moment, the light intensity of the first light beam and the light intensity of the second light beam are distributed through the light splitting module; the upper polar plate or the lower polar plate is used for irradiating the micro-fluidic chip; after the second light beam irradiates the lower polar plate, dielectrophoretic force is generated through the photoconductive layer to drive the cells to move, so that the cells can be effectively moved, and the light intensity can be reduced to prevent the cells from being damaged. The first light beam irradiates the upper polar plate and penetrates through the upper polar plate to irradiate specific cells in the micro-channel, so that the specific cells or secretions of the specific cells can be effectively and conveniently identified and observed. By arranging the first light path module and the second light path module, the paths of the first light beam and the second light beam can be changed, so that corresponding adjustment can be performed based on specific requirements, and control and observation of different types of micro-fluidic chips can be met.

The invention provides an electric separation device and method for removing solid catalyst particles in catalytic slurry oil, and belongs to the technical field of slurry oil solid removal and petrochemical engineering, the device comprises an outer cylinder, an inner cylinder and a central metal rod which are coaxially arranged, the inner cylinder is connected with a high-voltage power supply to form a non-uniform electric field, and the outer cylinder and / or the inner cylinder are / is of a variable-diameter structure; due to the design, the electric field gradient is enhanced in a variable-diameter area, so that the dielectrophoretic force acting on the superfine catalyst particles is increased; meanwhile, according to the fluid continuity principle, the flow speed of the oil slurry decreases progressively due to increase of the cross section area of a channel in the flowing process from top to bottom, the particle retention time is prolonged, and fluid drag force is reduced. Through the synergistic effect of the two effects, the removal efficiency of superfine particles with the diameter smaller than 1 micron is remarkably improved. The device is simple in structure, does not need a complex control system, can achieve efficient and stable solid removal under moderate electric field intensity, and is suitable for various complex working conditions.

The application provides a cell inertia sorting device and method based on dielectric enhancement, and belongs to the technical field of cell filtering and sorting. The device comprises a spiral structure inertia sorting module and a dielectrophoresis enhancement sorting module. Specifically, the device comprises a PDMS cover sheet layer, an Ag-PDMS three-dimensional electrode layer, an ITO wire layer and a glass substrate layer. The spiral structure inertia sorting module can focus cells of different sizes at different balance positions. In the dielectrophoresis enhancement sorting module, a long strip-shaped three-dimensional electrode group and a semicircular three-dimensional electrode are designed to provide strong and stable dielectrophoresis force for cells. Since different cells are subjected to dielectrophoresis forces of different sizes and directions, different directional deviations are generated, so that the separation distance of the two kinds of cells is increased, and the inertia sorting effect is enhanced. The inertia technology and the dielectrophoresis technology are organically combined, the sorting precision is improved, and high-precision and high-throughput separation between different cells is realized.

The utility model relates to the field of bipolar microfluidics, and discloses a bipolar microfluidics production device which comprises a bearing table, a microfluidics main body is fixedly arranged on the upper surface of the bearing table, a chip is fixedly arranged on the upper surface of the bearing table, and a blue light tower is fixedly arranged on the upper surface of the bearing table. According to the device, through the arrangement of the micro-fluidic main body, primary fluid treatment can be carried out on liquid, through the arrangement of the chip, high-precision control can be carried out on small particles and cells in the fluid through the synergistic effect of an electric field generated by the bipolar electrode, electroosmotic flow and dielectrophoretic force, and through the arrangement of the heat preservation sleeve and the temperature adjusting instrument, high-precision control can be carried out on the small particles and cells in the fluid. The temperature of the fluid in transmission can be accurately controlled, the fluid can be specifically treated through the arrangement of a blue light tower, the activity of a biological sample and the stability of chemical substances in the fluid can be effectively protected through the arrangement of a low-temperature collector, and the fluid can be uniformly treated through the arrangement of a blue light emission vessel and a rotor.

The invention discloses a liquid-like surface large particle multi-type solid-liquid control method based on a pyroelectric effect. The method comprises the following steps: step 1, pretreating a pyroelectric wafer material; step 2, performing liquid phase modification on the pyroelectric wafer; step 3, preparing a photo-thermal response material; step 4, operating platform combination; step 5, directional control of liquid drops; and step 6, performing directional control on the solid particles. The control platform designed by the invention is formed by combining three layers of materials in a blocking manner, the preparation process is simple, the solid-liquid control operation method is simple and convenient, and the production cost is relatively low. According to the control platform, a liquid-like surface and a pyroelectric material are combined, on the basis that the friction force between a solid and the surface of the platform is greatly reduced on the liquid-like surface, local charge gradient is generated on the surface by means of the response mechanism of the pyroelectric material, and direct control over the solid material is achieved under the action of dielectrophoretic force; the innovative microfluidic platform and solid control mode greatly widen the practical application of the technical field of microfluidic.

The invention relates to determining the frequency of TTfield therapy based on electrical characteristics of targeted cancer cells. Cancer treatment using TTField (Tumor Treatment Field) may be tailored for each individual subject, by obtaining cancer cells from the subject, determining electrical characteristics of the cancer cells (e.g., dielectrophoretic force, cell membrane capacitance, etc.), determining a frequency for TTField based on the determined electrical characteristics, and treating cancer by applying TTField to the subject at the determined frequency. Further, cancer treatment may be planned for each individual subject, by obtaining cancer cells from the subject, determining electrical characteristics of the cancer cells, predicting whether TTField will be effective to treat cancer based on the determined electrical characteristics, and treating the subject by applying TTField if the prediction indicates that TTField is effective.

The present invention discloses a multi-field composite non-contact polishing device and method, which can achieve high-efficiency and high-quality polishing of workpieces through the composite of temperature field, electric field, magnetic field, chemical field, and mechanical field. The temperature control system can achieve constant control of the temperature of the non-contact polishing liquid, ensuring that the polishing liquid always maintains a good shear thickening effect in a constant temperature field during the polishing process. The abrasive particles polarized in the dielectric medium by the alternating power supply are moved by the dielectrophoretic force, preventing the sedimentation of most of the abrasive particles during the polishing process and improving the polishing removal rate. A magnetic field is applied between the polishing tool and the workpiece to promote the adsorption of magnetic abrasive particles in the polishing liquid on the processed surface of the workpiece, allowing the abrasive particles to enter more corners of the complex surface of the workpiece, and increasing the pressure of the abrasive particles on the workpiece surface during polishing, thereby improving the polishing efficiency. The oxidant in the polishing liquid removes the surface oxide layer through the mechanical action of the abrasive particles, and this cycle can eventually achieve a high-quality surface.

The invention discloses a micro-fluidic chip and a manufacturing method of the micro-fluidic chip. According to the micro-fluidic chip, the photoconductive area is formed between the at least two first supporting pieces, the photoconductive layer is arranged in the photoconductive area, light beams irradiate the lower polar plate, dielectrophoretic force is generated through the photoconductive layer to drive cells to move, and the cells can be effectively controlled; the supporting structure between the upper polar plate and the lower polar plate is divided into the first supporting pieces and the second supporting pieces, the at least two first supporting pieces are adjacently arranged, a microfluid channel is formed between the two adjacent first supporting pieces, and the second supporting pieces are used for forming auxiliary supporting between the upper polar plate and the lower polar plate, so that the stability of the micro-fluidic chip can be effectively improved; besides, the second supporting piece is arranged on the outer side of the first supporting piece, so that the movement of cells in the microfluid channel is not interfered, and a stable and uniform supporting effect can be provided on the outer side.

The control voltage correction method corrects a control voltage that causes a dielectrophoretic force to act on dielectric particles contained in the first fluid injected into a fluid chip. The control voltage correction method includes measuring an impedance between first electrodes in a fluid chip through the first fluid or a second fluid, calculating a correction coefficient based on the measured impedance and a fluid impedance derived by a mathematical formula, and correcting a control voltage based on the correction coefficient. The fluid impedance indicates an impedance of the first fluid or the second fluid. The first fluid is a fluid containing dielectric particles and other particles. The second fluid is the same type of fluid as the fluid excluding the dielectric particles and the other particles from the first fluid.

The invention discloses a battery charging and discharging method and device and a medium, relates to the field of batteries, and automatically triggers capacity recovery of the battery when the battery reaches a preset recovery condition. And pulse parameters are determined in real time according to the current capacity fading rate of the battery, so that the configuration and application of the repair pulse are carried out. A restoration pulse is applied to an electrode of the battery, so that a neutral particle of an isolated silicon-lithium compound in the battery can generate a dielectrophoresis effect under the action of an electric field generated by the restoration pulse, and the isolated silicon-lithium compound is controlled to move towards the electrode based on the action of dielectrophoresis force, so that the isolated silicon-lithium compound is in contact with a binder in the electrode; and the conductive network can be adhered to the conductive network of the electrode again under the action of the adhesive, and is reconnected with the conductive network to participate in subsequent electrochemical reaction, so that the capacity recovery of the electrode is realized. The pulse parameters are determined according to the real-time capacity attenuation rate of the battery, and can adapt to the real-time characteristics of the battery to realize capacity recovery, so that the cycle life of the battery is effectively prolonged.

Methods of selectively positioning a micro-object in a microfluidic device are described in this application. The microfluidic device can comprise an enclosure having an inlet, an outlet, and a flow region connecting the inlet and outlet, and an electrode activation substrate having a photoconductive layer. The methods of selectively positioning can comprising: projecting a first light beam on an electrode activation substrate of the microfluidic device, wherein the first position is proximal to the first micro-object, and wherein the first light beam activates a positive dielectrophoresis (DEP) force within the enclosure sufficient to capture the first micro-object; and projecting a second light beam upon a second position on the electrode activation substrate, wherein the second position is adjacent to or at least partially surrounding the first position, without overlapping the first position, the second light beam activating a positive DEP force within the enclosure sufficient to capture second micro-objects other than the first micro-object. The methods of selectively positioning can further comprise moving the first light beam towards a third position on the electrode activation substrate, wherein the DEP force activated by the first light beam is sufficient to move the first micro-object to the third position. Optionally, the methods can include moving the second light beam in relation to the first light beam to prevent micro-objects other than the first micro-object from being captured by the first light beam. Other embodiments are described.

The invention relates to the technical field of electronic measuring instrument manufacturing, and discloses a surface enhanced Raman detection system and method based on electric field regulation and control plasmon coupling. The system comprises a micro-fluidic chip assembly with a micron-order interdigital electrode, an electric field modulation driving unit, a photoelectric signal acquisition unit and a phase lock analysis and arbitration processing unit, and the electric field modulation driving unit applies periodic alternating current driving voltage to the interdigital electrode; the SERS active nanoparticles are driven by dielectrophoretic force to generate periodic aggregation and diffusion motion which is twice of the basic frequency, the phase-locked analysis and arbitration processing unit extracts the frequency doubling amplitude in the spectral signal as a concentration characterization value, and outputs a result only when a phase lag component is located in a preset effective interval. SERS signals are stripped from direct-current noise, and the measured signal-to-noise ratio and reliability are improved.

The utility model provides a kind of cell well plate, from bottom to top sequentially include substrate, middle layer, hydrophobic layer, the middle layer is equipped with a plurality of sample wells, the sample well is communicated with the substrate, electrode is provided on the substrate, and the electrode is located the sample well below;The electrode is used to generate uneven electric field, so that cell or bacteria is inclined to the side movement of uneven electric field and is settled in the bottom of the sample well;The middle layer is used to clean sample liquid;The hydrophobic layer is used to keep sample in the sample well and sample well between not connected and flows in series.The utility model adds electric field below well plate, improves cell settling velocity by dielectrophoresis force effect, and cell (or bacteria) is not easy to lose under the action of dielectrophoresis force after settling, and has better retention rate in laminar flow cleaning process.

The invention relates to a cell three-dimensional rotation and electroporation micro-fluidic chip based on alternating current electric field regulation and control, which comprises a first PDMS layer, a second PDMS layer and a third PDMS layer which are sequentially stacked, a first PDMS channel and a second PDMS channel are arranged in the first PDMS layer, a third PDMS channel is arranged in the second PDMS layer, and the third PDMS channel is arranged in the third PDMS layer. A fourth PDMS channel, a fifth PDMS channel, a fluid inlet and a fluid outlet are formed in the third PDMS layer. According to the embodiment of the invention, the electric field is applied to the four exciting electrodes to form the rotating electric field, the negative dielectrophoresis force generated by the rotating electric field generates the rotating torque on the cells, the cells are controlled to rotate three-dimensionally, and the cells are driven to rotate three-dimensionally in different directions and at different speeds by changing the parameters of the applied electric field, so that the three-dimensional rotation of the cells is realized. Therefore, many genetic information and structural details of observed cells and particles can be observed, the applied voltage is small, additional modification on the cells is not needed, and the control is accurate.

The application discloses a method for decelerating and / or capturing single molecules by dielectrophoresis force based on a nanopore device. The method comprises the following steps: preparing a solid-state nanopore film; taking the solid-state nanopore film as a diaphragm for separating an anode and a cathode of an electrolytic cell to obtain a nanopore sequencing device; detecting by using the nanopore sequencing device; adding a single molecule to be detected into a negative electrode grounding chamber of the electrolytic cell; during the detection, applying a direct current voltage at two ends of the diaphragm, introducing an alternating current voltage through a conductive layer, and respectively providing an electric field force and a dielectrophoresis force for the single molecule, so as to respectively serve as a driving external field for the single molecule to pass through a nanopore in the solid-state nanopore film and a capturing force for the single molecule to stay in the nanopore; and by controlling the dielectrophoresis force to be greater than or approximately equal to the electric field force, the single molecule is decelerated or captured. The application expands the measurement range of the nanopore to-be-detected substance by using dielectrophoresis, and can realize real-time controllable capturing and deceleration perforation of the single molecule, so that the detection time resolution is greatly improved.