38 results about "Cytometry" patented technology

The present disclosure provides improved and useful techniques for cross-standardization of flow cytometry instruments and, particularly, spectral flow cytometry instruments. Aspects of the disclosure include methods of calibrating a flow cytometer having a plurality of fluorescence channels. Methods of interest utilize calibration sets of bead populations, wherein each bead population of the calibration set includes a different fluorophore attached to a surface thereof and the calibration set includes a number of bead populations that is less than the number of fluorescence channels of the flow cytometer. Flow cytometers, non-transitory computer-readable storage media, and kits including, e.g., calibration sets of bead populations for carrying out the subject methods are also provided.

An analytical method of and system for monitoring cell status whereby excitation energy is focused to an excitation cytometry volume in a cell sample to fluoresce first and second fluorophores of a cell. Fluorescence signals from the first and second fluorescing cell fluorophores are directed to a detection subsystem. A first peak in fluorescence intensity for the first fluorophore is detected as is a second peak in fluorescence intensity for the second fluorophore. The fluorescence signals are processed to identify when the first and second peaks occur substantially simultaneously indicating the presence of a cell at the excitation cytometry volume instead of background fluorescence and in response, the cellular status of the cell is measured using the intensity of the levels of the first and second peaks.

Described herein are techniques for predicting whether a subject will respond to an immune checkpoint inhibitor (ICI) therapy based on RNA expression data and cytometry data obtained for the subject. In some embodiments, the techniques include: obtaining the RNA expression data, the RNA expression data having been previously obtained from a tumor sample from the subject; selecting, using the RNA expression data, an MF profile type for the tumor sample; obtaining the cytometry data, the cytometry data having been previously obtained from a blood sample from the subject; determining, using the cytometry data, a G2 score for the blood sample, wherein the G2 score is indicative a likelihood that the blood sample is of a Primed (G2) immunoprofile type of multiple immunoprofile types; and predicting, based on the selected MF profile type and the G2 score, whether the subject will respond to the ICI therapy.

A method for determining whether a subject is at risk for having a progressing or high-grade pre-invasive lesion, nodule or small mass, or having a solid malignant tumour is described the method comprising: (i) determining a ratio of activated and / or exhausted T cells:naive and / or resting T cells in a sample of blood obtained from the subject, wherein the determining comprises analysing T cells using cytometry to detect the presence or absence of a panel of biomarkers comprising Ki67 and CD39, or (ii) determining a ratio of activated and / or exhausted T cells:T cells which are not activated and / or exhausted T cells in a sample of blood obtained from the subject, wherein the determining comprises analysing T cells using cytometry to detect the presence or absence of a panel of biomarkers comprising Ki67 and CD39, (iii) determining a proportion of activated and / or exhausted T cells as a percentage of T cells in a sample of blood obtained from the subject, wherein the determining comprises analysing T cells using cytometry to detect the presence of a panel of biomarkers comprising Ki67 and CD39, and / or (iv) determining a proportion of activated and / or exhausted T cells as a percentage of T cells in a sample of blood obtained from the subject, wherein the T cells are CD4 T cells, and wherein the determining comprises analysing T cells using cytometry to detect the presence of a panel of biomarkers comprising FoxP3.

An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and / or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.

Systems, methods, and apparatuses for performing real-time data analytics. One apparatus includes at least one electronic processor and at least one memory storing instructions executable by the at least one electronic processor. The at least one electronic processor is configured to obtain a sequence of cytometry data output by a cytometry instrument, normalize a first data point in the sequence of cytometry data using a set of normalization parameters determined based on a queue of cases belonging to a predetermined class, update the queue of cases belonging to the predetermined class with the normalized first data point in response to determining, using a machine learning model, the normalized first data point belongs to the predetermined class, and normalize a second data point in the sequence of cytometry data using an updated set of normalization parameters determined based on the updated queue of cases belonging to the predetermined class.

An objective lens unit for a microscope is disclosed. The objective lens unit is suitable to be used when at least partially submerged in a liquid sample. The objective lens unit includes an immersion lens positioned adjacent a distal front end of the enclosure of the objective lens unit. The immersion lens is arranged and configured to receive light through a front end optical aperture. An objective lens system is arranged inside an enclosure and proximal from the immersion lens. The objective lens system is configured to collect light received by the immersion lens through the front end optical aperture. The objective lens system includes a motorless automated adjustable lens, in particular a tuneable lens, configured to amend the focal length of the objective lens system relative to the immersion lens.

Systems, methods, and apparatuses for performing real-time cytometry data analysis. One apparatus includes at least one electronic processor and at least one memory storing instructions executable by the at least one electronic processor. The at least one electronic processor is configured, through execution of the instructions, to obtain flow cytometry data generated by a cytometry instrument representing cells of multiple categories, generate a feature vector representation based on the flow cytometry data using a plurality of self-organizing maps (SOMs), wherein each SOM corresponds to a different category of multiple categories, and predict each of one or more target labels of the cells by applying each of one or more regression models to the feature vector representation.

A device for directing illumination for a sample volume in microscopy, such as in-situ cytometry. The device includes a body having a distal front end with an optical aperture provided therein, and a reflector. The reflector is arranged distal from the front end and at a non-zero distance from the optical aperture and is configured to receive light emitted through the front end and reflect at least part of the light received from the front end back into the optical aperture. The reflector is connected to the body by at least one cantilevering strut. The device may be part of an objective lens unit.

An apparatus for guiding illumination for a sample volume in microscopy, in particular in in situ cytometry. The device includes a body (611) having a distal front end (62) and an optical aperture (63) disposed in the front end, and a reflector (621). And a reflector disposed distal of the front end at a non-zero distance from the optical aperture, the reflector configured to receive light emitted through the front end and to reflect at least a portion of the light received from the front end back into the optical aperture. The reflector is connected to the body by at least one cantilevered strut. The proposed device may be part of an objective unit (6).

A pump is configured for peristaltic pumping that facilitates high throughput cytometry. The pump includes a rotary head, an occlusion bed, and an engagement mechanism configured to perform functions. The functions include applying a first force that compresses a flexible tube between the occlusion bed and the rotary head in response to a second force applied to the engagement mechanism, wherein the second force is not parallel with the first force. The functions also include discontinuing applying the first force in response to a third force applied to the engagement mechanism, wherein the third force is opposite in direction to the second force.

In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell / particle.

This disclosure provides a method for identifying and counting unlabeled (native) blood cells and analytes in whole blood. In particular, this disclosure relates to devices used within the body equipped with microfluidic cytometry optical sensors and detectors that recognize analytes or histological architecture from within a patient to provide clinically actionable data related to the subject's health.

The invention provides a single-tube multi-allergen specific basophilic granulocyte detection method based on multicolor flow cytometry, and belongs to the technical field of immunodetection. Through a single-tube multicolor flow cytometry technology, rapid and synchronous quantitative analysis of various allergen-specific basophilic granulocytes in peripheral whole blood is realized, and the detection efficiency and flux are remarkably improved; the method is easy and convenient to operate, small in needed sample size and especially suitable for children and other people with blood sampling difficulty, and the limitation of clinical application is greatly reduced; by directly measuring cell populations closely related to anaphylaxis, a more intuitive and reliable auxiliary diagnosis basis is provided for IgE-mediated anaphylaxis diseases, the immune state change of a patient in the treatment process can be effectively monitored, and a new technical support is provided for clinical precise intervention and curative effect evaluation.

The present invention relates to a method for quantifying target polynucleotides, particularly RNA molecules such as 16S rRNA, through a process that converts the abundance of target molecules into the abundance of predefined barcoded oligonucleotides The invention enables reduced nonspecific binding, protocol flexibility with pausing and re-entry points, and versatile, optionally multiplexed data acquisition including flow-cytometry-like or imaging-based measurements.

This invention discloses a zero-field-based flow cytometry fluorescence detection method and system. The method includes: when a target material specifically bound to nanodiamonds flows through the detection region of a sample channel, controlling at least one of the intensity, frequency, and on / off state of a microwave field to induce periodically varying fluorescence signals emitted by the NV centers within the nanodiamonds; the magnetic field strength in the detection region satisfies the zero-field condition; an excitation light module excites the NV centers in the detection region of the sample channel; a microwave radiation module provides a microwave field to the detection region of the sample channel, and the microwave radiation signal provided by the microwave field has a fixed frequency; a photodetector module collects the fluorescence signal at a preset collection frequency; and a data processing module determines whether the target analyte contains the biomolecule to be detected based on the fluorescence signal. The technical solution provided by this invention improves the detection sensitivity of the fluorescence detection method.

The application discloses a platelet detection kit based on multi-parameter flow cytometry and a use method thereof, relates to the technical field of in-vitro diagnosis, and covers the core pathological links of thrombosis and downstream key events of thrombosis by simultaneously detecting the proportion of PNH clone cells, the platelet activation state and the proportion of platelet-leukocyte aggregates (PLAs), thereby efficiently and accurately evaluating the thrombosis risk of PNH patients. The application directly detects cell surface markers and cell-cell interaction by using multi-color flow cytometry, can early and sensitively identify abnormal activation signals, and avoids the limitations of traditional coagulation indexes (such as D-dimer) in PNH thrombosis early warning.

A measurement system is disclosed which includes a spot-traversal system for causing relative motion between a sample and an irradiation spot in a first direction, wherein the sample includes one or more fluorescent markers having respective fluorescence wavelengths, a gating system configured to provide a gating signal based at least in part on resultant light substantially at a wavelength of the irradiation spot, and an optical detection system configured to detect fluorescent light from at least some of the fluorescent markers irradiated by the irradiation spot, and provide detection signal(s) representing the fluorescent light detected concurrently with a gate-open condition of the gating signal.

Systems, methods, and computer readable storage mediums for performing multidimensional cytometry data analysis are described. The method includes filtering multidimensional cytometry data, based on a signal strength within each dimension of the plurality of dimensions, to select representative dimensions from the plurality of dimension, selecting an initial region within the representative dimensions of the multidimensional cytometry data. The initial region can include a bin with a maximum significance level. A significant region of the representative dimensions, presenting a response different from a reference response, can be determined by expanding the initial region to regions with a set significance. A display including the significant region of the representative dimensions of the multidimensional cytometry data can be generated.

A pump is configured for peristaltic pumping that facilitates high throughput cytometry. The pump includes a rotary head, an occlusion bed, and an engagement mechanism configured to perform functions. The functions include applying a first force that compresses a flexible tube between the occlusion bed and the rotary head in response to a second force applied to the engagement mechanism, wherein the second force is not parallel with the first force. The functions also include discontinuing applying the first force in response to a third force applied to the engagement mechanism, wherein the third force is opposite in direction to the second force.

An objective lens unit (6) for a microscope is disclosed. The objective lens unit is adapted for use when at least partially immersed in a liquid sample. The objective unit includes an immersion lens (64, 642) positioned adjacent a distal front end (62) of a housing of the objective unit. The immersion lens is arranged and configured to receive light through a front end optical aperture (63). An objective lens system (65, 66) is disposed inside the housing (61) and proximal to the immersion lens (64, 642). The objective lens system is configured to collect light received by the immersion lens (64, 642) through the front end optical aperture (63). The objective lens system comprises a motorized auto-adjustment lens (65), in particular an adjustable lens, configured to be able to correct the focal length of the objective lens system with respect to the immersion lens.

An analytical method and system for monitoring cellular status wherein excitation energy is focused into an excitation cytometry volume within a cell sample that is located within a bioreactor or fermenter to induce optical signals from intracellular compounds. The resulting optical signals are directed to a detection subsystem that has at least two detection channels. One channel detects the elastic (Rayleigh or Mie) scattering signal from the cell that identifies the presence of the cell within the excitation volume. Another channel detects the fluorescence and / or Raman scattering signal of intracellular compounds of the cells. The elastic scattering signals associated with individual cells are used as event-triggers to gate the detection of the intracellular fluorescence and / or Raman signal to eliminate the background noises from the suspension media. The intracellular fluorescence and / or Raman scattering signal are used to characterize cellular status based on the fluorescence intensities at specific fluorescence wavelengths and peak analysis of Raman scattering spectra.

A method for determining whether a subject is at risk for having a progressing or high-grade pre-invasive lesion, nodule or small mass, or having a solid malignant tumour is described. The method involves analysing the proportion of T cells in a sample of blood obtained from the subject which are activated and / or exhausted T cells by analysing a trait of the T cells, e.g., via analysing biomarker expression using cytometry. In some instances, a plurality of traits is analysed and combined, e.g., via analysing biomarker expression using cytometry in combination with analysing the diversity or clonality of the blood TCR repertoire using TCR-seq.

The application belongs to the technical field of antibody preparation, and particularly relates to an antibody against B lymphocyte antigen CD19, an antibody conjugate and application thereof in immune detection. The amino acid sequences of CDR1-3 on the light chain variable region of the antibody are respectively shown as SEQ ID NO. 3-5, and the amino acid sequences of CDR1-3 on the heavy chain variable region are respectively shown as SEQ ID NO. 8-10. The antibody of the application has high recognition sensitivity and binding affinity to CD19 protein naturally expressed by human and monkey cells, has no cross reaction or weak non-specific binding to non-target proteins on cells, is beneficial to improving the sensitivity, specificity, accuracy and detection precision of detection, provides an antibody tool with good performance, reliability and practicality for the fields of immune cell subpopulation typing and B lymphocyte function identification, and is particularly suitable for the establishment and application of a flow cytometry and multi-color flow cytometry detection system.

The invention in particular relates to a counting method for microbiological control, comprising: cytometry analysis (101) of a matrix comprising a given volume of a sample, said sample having been labelled beforehand using a labelling protocol; and obtaining (102) a multidimensional data table collating objects detected during the cytometry analysis, characterised in that the method comprises a step of applying a counting algorithm comprising at least the following steps: assigning (105) a quantitative score contained in a range of values ranging from [0 to 1] to each object detected during the analysis, depending on its probability of belonging to a defined "microorganism" class; and summing (108) the scores obtained in the preceding step, and in that the method comprises a step of: counting (109) microorganisms based on the sum of the scores.

Methods that include tagging at least one cell with a MXene, the cell optionally being an immune cell; and detecting at least one component of the MXene using one or more of single-cell mass cytometry by time-of-flight (CyTOF), imaging mass cytometry (IMC), and ion beam imaging (MIBI-TOF). Systems that include a cell tagged with an amount of a MXene; and a detection train configured for at least one of time of flight (CyTOF), imaging mass cytometry (IMC), and ion beam imaging by time-of-flight (MIBI-TOF) that detects the MXene. Methods that include tagging a population of cells with at least one MXene: and processing the population of cells with at least one of time-of-flight (CyTOF), imaging mass cytometry (IMC), and ion beam imaging by time-of-flight (MIBI-TOF) that detects the at least MXene; and relating the detection of the at least one MXene to a characteristic of the population of cells.

An example method performed via a computing device for providing support to an imaging flow-cytometry instrument includes receiving flow-cytometry event data and images representing a plurality of events detected with the imaging flow-cytometry instrument and corresponding to a sample including cells and beads. The method also includes selecting a group of events by applying a set of gating operations to the plurality of events. The group of events includes bead events. The method also includes processing a subset of the images corresponding to the selected group of events with a machine learning model to determine a total number of beads in the subset of the images.

The invention relates to a microfluidic device and system for detecting and / or measuring at least one nucleotide sequence. The invention also relates to a method of detecting a nucleotide sequence by microfluidic impedance cytometry, and detecting genetic variation in individuals by allele-specific amplification by PCR and microfluidic impedance cytometry.

Non-transient processor-readable media, when executed by the processor, store instructions causing the processor to receive a first data array associated with a first site-metric instrument at a first time point. The first data array includes a set of scattered signal data points, each containing data representing at least one low forward scattered signal output, at least one high forward scattered signal output, at least one low side scattered signal output, and at least one high side scattered signal output. At a second time point following the first, the site-metric instrument parameters are adjusted based on the first data array, and / or the first site-metric measurement of the first site-metric instrument is compared with a second site-metric measurement of a different site-metric instrument, based on at least one of the first data array or a second data array associated with a second site-metric instrument.

The invention relates in particular to an enumeration method for microbiological control comprising: Analysis (101) by flow cytometry of a matrix comprising a given volume of a sample, said sample having been previously labeled by a labeling protocol; Obtaining (102) a multidimensional data table presenting objects detected during the flow cytometry analysis, characterized in that the method comprises a step of applying an enumeration algorithm comprising at least the following steps: Assignment (105) of a quantitative score within a range of values ​​from [0 to 1] to each object detected during the analysis according to its probability of belonging to a determined "microorganism" class; Summing (108) the scores obtained in the preceding step; and in that the method comprises a step of: Enumeration (109) of microorganisms from the sum of the scores. Figure 1