95 results about "Cytometry" patented technology

Bead-in-particles formats and their use in cytometric applications are described. In one aspect, the disclosure provides a bead-in-particle comprising an outer hydrogel body and an inner hydrogel bead. As described herein, the bead-in-particle is selectively tuned to have at least one morphological property substantially similar to subcellular macrostructures of interest present in a target cell. In one aspect of the disclosure, the bead-in-particle are used as a calibration reagent for the detection of a target cell in a sample, such as by image-based flow cytometry.

The present disclosure provides systems and methods for analyzing a mixed population of cells. In particular, the present disclosure provides systems and methods for digital cytometry of a biological sample, digital analysis of a biological sample, digital purification of a biological sample, evaluation of a disease in an individual, and prediction of a clinical outcome of a disease therapy.

The present disclosure relates generally to an autonomous cell imaging and modeling platform, and more specifically to machine-learning techniques for using microscopy imaging data to continuously study live biological cells. The autonomous cell imaging and modeling platform can be applied to evaluate various cellular processes, such as cellular differentiation, optimization of cell culture (e.g., in-plate cytometry), disease modeling, histopathology imaging, and genetic and chemical screening, using a dynamic universal imaging system. In some embodiments, the platform comprises a set of label-free computational imaging techniques, self-supervised learning models, and robotic devices configured in an autonomous imaging system to study positional and morphological characteristics in particular cellular substructures of a cell culture in an efficient and non-destructive manner over time.

The utility model discloses a sampling device for flow cytometry, which relates to the technical field of cytometry and comprises a bottom plate and a rotating rod, the rotating rod is rotatably arranged on the upper surface of the bottom plate, a cross rod is fixedly arranged at the upper end of the rotating rod, and mounting shells are fixedly arranged at two ends of the cross rod. Mounting blocks are clamped to the inner sides of the two mounting shells correspondingly, a placement plate is fixedly arranged on the upper sides of the mounting blocks, a plurality of limiting rings are arranged above the placement plate, the two ends of the lower sides of the limiting rings are fixedly connected with the placement plate through connecting rods correspondingly, a vertical rod is fixedly arranged on one side of the upper surface of the bottom plate, and the vertical rod is fixedly connected with the lower side of the bottom plate. And wedge-shaped blocks are fixedly arranged at the upper ends of the vertical rods, wedge-shaped grooves are formed in the lower ends of the mounting blocks on the two sides, a through groove is formed in the middle of the lower side of the mounting shell, and the wedge-shaped blocks are connected with the corresponding wedge-shaped grooves in a clamped mode. According to the utility model, loading and reloading can be carried out synchronously, so that the time wasted by reloading can be reduced, and the working efficiency is improved.

In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

The invention relates to the technical field of thallus quantity detection, in particular to a primer pair and a kit for detecting the number of lactobacillus brevis thalli in a postbiotic preparation and application of the primer pair and the kit. The primer pair provided by the invention has the advantages of strong specificity and high amplification efficiency, and can be used for carrying out qPCR quantitative detection on the quantity of lactobacillus brevis in the metastatic preparation. As the traditional flow cytometry and the plate cell counting method are not suitable for detecting the number of thalli in the metagen preparation, the qPCR quantitative method provided by the invention can be used for rapidly and accurately carrying out quantitative analysis on the number of lactobacillus brevis thalli in the metagen in a relatively short time, and the result is compared with a plate counting result; compared with the prior art, the qPCR counting method has the advantages that the detection result difference does not exceed one order of magnitude, counting does not distinguish viable bacteria and dead bacteria, and the qPCR counting method has the advantages of high specificity and high speed and can make up the limitation that a plate counting method is suitable for viable bacteria counting, so that the qPCR counting method provided by the invention has more advantages in the application of metagen counting.

In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

The present disclosure provides improved and useful techniques for cross-standardization of flow cytometry instruments and, particularly, spectral flow cytometry instruments. Aspects of the disclosure include methods of calibrating a flow cytometer having a plurality of fluorescence channels. Methods of interest utilize calibration sets of bead populations, wherein each bead population of the calibration set includes a different fluorophore attached to a surface thereof and the calibration set includes a number of bead populations that is less than the number of fluorescence channels of the flow cytometer. Flow cytometers, non-transitory computer-readable storage media, and kits including, e.g., calibration sets of bead populations for carrying out the subject methods are also provided.

This disclosure concerns a cytometry system including a handling system that enables presentation of single cells to at least one laser source. The laser source is configured to deliver light to a cell within the cells in order to induce bond vibrations in the cellular DNA. The system further includes a detection facility that detects the signature of the bond vibrations, wherein the bond vibration signature is used to determine the folding or packing of the DNA.

In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a spectral flow cytometer with a least three excitation lasers and thirty-eight color detectors. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

Provided relates to the field of cytometry, specifically to flow cytometric methods and kits for improved diagnosis, prognosis and monitoring of tumors and other lesions involving immune cell infiltration. Further provided are embodiments of the subject matter which relate to compositions and methods providing high resolution quantitative means for immunophenotyping and immune modeling, and for identification of disease prognostic and therapy predictive biomarkers.

Here, the inventors have developed a spectral flow cytometry assay to measure heme biosynthesis. By using this new assay on melanoma cell lines, they observed a direct correlation between high PPIX levels in differentiated MITFhigh cells and a protection against ferroptosis. Conversely, cell lines with low PPIX levels were associated with a dedifferentiated MITFlow phenotype enriched in stem cell markers and more sensitive to ferroptosis inducers. They also found that inhibition of PPIX biosynthesis in differentiated melanoma cells synergizes with iron overload to induce lipid peroxidation and tumor cell death. Finally, they show that decreased levels of PPIX linked to increased ferroptosis sensitivity can be acquired in vivo in melanoma tumors that relapse after anti-MAPK targeted therapies. The present invention relates to a method of determining whether a subject has or is at risk of having melanoma ferroptosis sensitivity and targeted therapy resistance comprising i) determining the level of protoporphyrin IX (PPIX) in a biological sample obtained from the subject and ii) comparing the level determined at step i) with a predetermined reference value: wherein if the level of the PPIX determined at step (i) is lower than the predetermined reference value is indicative that the said patient is having melanoma ferroptosis sensitivity and targeted therapy resistance or wherein if the level of the PPIX determined at step (i) is higher than the predetermined reference value is indicative that the said patient is having melanoma ferroptosis resistance and targeted therapy sensitivity. The present invention also relates to a method for treating resistant melanoma in a subject in need thereof comprising a step of administering said subject with a therapeutically effective amount of a ferroptosis inducer.

An analytical method of and system for monitoring cell status whereby excitation energy is focused to an excitation cytometry volume in a cell sample to fluoresce first and second fluorophores of a cell. Fluorescence signals from the first and second fluorescing cell fluorophores are directed to a detection subsystem. A first peak in fluorescence intensity for the first fluorophore is detected as is a second peak in fluorescence intensity for the second fluorophore. The fluorescence signals are processed to identify when the first and second peaks occur substantially simultaneously indicating the presence of a cell at the excitation cytometry volume instead of background fluorescence and in response, the cellular status of the cell is measured using the intensity of the levels of the first and second peaks.

Described herein are techniques for predicting whether a subject will respond to an immune checkpoint inhibitor (ICI) therapy based on RNA expression data and cytometry data obtained for the subject. In some embodiments, the techniques include: obtaining the RNA expression data, the RNA expression data having been previously obtained from a tumor sample from the subject; selecting, using the RNA expression data, an MF profile type for the tumor sample; obtaining the cytometry data, the cytometry data having been previously obtained from a blood sample from the subject; determining, using the cytometry data, a G2 score for the blood sample, wherein the G2 score is indicative a likelihood that the blood sample is of a Primed (G2) immunoprofile type of multiple immunoprofile types; and predicting, based on the selected MF profile type and the G2 score, whether the subject will respond to the ICI therapy.

The invention relates to an acute leukemia (ALL) high-throughput flow cytometry kit, and belongs to the field of leukemia detection.16 ALL cell antigens are added on the basis of an original 8-color flow cytometry, 24 antigens in the same cell can be detected at the same time, the detection precision of minimal residual focus (MRD) is greatly improved, and the detection sensitivity is improved. The ALL cells are divided into 12 developmental stages, so that immunological typing during primary diagnosis and immunophenotype variation after bone marrow transplantation and CAR-T cell treatment can be more accurately recognized, and the method has very important guiding significance on evaluation of treatment effects of patients and adjustment of treatment schemes.

A method for determining whether a subject is at risk for having a progressing or high-grade pre-invasive lesion, nodule or small mass, or having a solid malignant tumour is described the method comprising: (i) determining a ratio of activated and / or exhausted T cells:naive and / or resting T cells in a sample of blood obtained from the subject, wherein the determining comprises analysing T cells using cytometry to detect the presence or absence of a panel of biomarkers comprising Ki67 and CD39, or (ii) determining a ratio of activated and / or exhausted T cells:T cells which are not activated and / or exhausted T cells in a sample of blood obtained from the subject, wherein the determining comprises analysing T cells using cytometry to detect the presence or absence of a panel of biomarkers comprising Ki67 and CD39, (iii) determining a proportion of activated and / or exhausted T cells as a percentage of T cells in a sample of blood obtained from the subject, wherein the determining comprises analysing T cells using cytometry to detect the presence of a panel of biomarkers comprising Ki67 and CD39, and / or (iv) determining a proportion of activated and / or exhausted T cells as a percentage of T cells in a sample of blood obtained from the subject, wherein the T cells are CD4 T cells, and wherein the determining comprises analysing T cells using cytometry to detect the presence of a panel of biomarkers comprising FoxP3.

An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and / or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.

Systems, methods, and apparatuses for performing real-time data analytics. One apparatus includes at least one electronic processor and at least one memory storing instructions executable by the at least one electronic processor. The at least one electronic processor is configured to obtain a sequence of cytometry data output by a cytometry instrument, normalize a first data point in the sequence of cytometry data using a set of normalization parameters determined based on a queue of cases belonging to a predetermined class, update the queue of cases belonging to the predetermined class with the normalized first data point in response to determining, using a machine learning model, the normalized first data point belongs to the predetermined class, and normalize a second data point in the sequence of cytometry data using an updated set of normalization parameters determined based on the updated queue of cases belonging to the predetermined class.

An objective lens unit for a microscope is disclosed. The objective lens unit is suitable to be used when at least partially submerged in a liquid sample. The objective lens unit includes an immersion lens positioned adjacent a distal front end of the enclosure of the objective lens unit. The immersion lens is arranged and configured to receive light through a front end optical aperture. An objective lens system is arranged inside an enclosure and proximal from the immersion lens. The objective lens system is configured to collect light received by the immersion lens through the front end optical aperture. The objective lens system includes a motorless automated adjustable lens, in particular a tuneable lens, configured to amend the focal length of the objective lens system relative to the immersion lens.

Systems, methods, and apparatuses for performing real-time cytometry data analysis. One apparatus includes at least one electronic processor and at least one memory storing instructions executable by the at least one electronic processor. The at least one electronic processor is configured, through execution of the instructions, to obtain flow cytometry data generated by a cytometry instrument representing cells of multiple categories, generate a feature vector representation based on the flow cytometry data using a plurality of self-organizing maps (SOMs), wherein each SOM corresponds to a different category of multiple categories, and predict each of one or more target labels of the cells by applying each of one or more regression models to the feature vector representation.

The invention relates to a new and time-saving method for determining disease-specific sensitivity and selectivity of test compounds in low volume samples based on high throughput flow-cytometry based cell recognition. The invention further relates to a test kit for performing the method.

A device for directing illumination for a sample volume in microscopy, such as in-situ cytometry. The device includes a body having a distal front end with an optical aperture provided therein, and a reflector. The reflector is arranged distal from the front end and at a non-zero distance from the optical aperture and is configured to receive light emitted through the front end and reflect at least part of the light received from the front end back into the optical aperture. The reflector is connected to the body by at least one cantilevering strut. The device may be part of an objective lens unit.

An apparatus for guiding illumination for a sample volume in microscopy, in particular in in situ cytometry. The device includes a body (611) having a distal front end (62) and an optical aperture (63) disposed in the front end, and a reflector (621). And a reflector disposed distal of the front end at a non-zero distance from the optical aperture, the reflector configured to receive light emitted through the front end and to reflect at least a portion of the light received from the front end back into the optical aperture. The reflector is connected to the body by at least one cantilevered strut. The proposed device may be part of an objective unit (6).

A pump is configured for peristaltic pumping that facilitates high throughput cytometry. The pump includes a rotary head, an occlusion bed, and an engagement mechanism configured to perform functions. The functions include applying a first force that compresses a flexible tube between the occlusion bed and the rotary head in response to a second force applied to the engagement mechanism, wherein the second force is not parallel with the first force. The functions also include discontinuing applying the first force in response to a third force applied to the engagement mechanism, wherein the third force is opposite in direction to the second force.

In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell / particle.

A flow cytometry system is provided. The system comprises: a flow cell or flow system configured to accommodate the flow of a fluid including one or more objects of interest; a light source configured to illuminate the object of interest; a plurality of tilted lenses each configured to capture a set of 2D images of the object as it flows through the flow cell or flow system and together configured to translocate the 2D images into a linear array of images perpendicular to the flow of the fluid; a sensor configured to integrate each set of 2D images; wherein the system is configured to read out and process the data from the linear array of images in order to determine one or more spatial parameters of the objects of interest. A method for 3D imaging an object is also provided.

The invention provides a method for inducing immature dendritic cells from mononuclear cells. After the peripheral blood mononuclear cells from different donors are subjected to cold treatment, the mononuclear cells are induced and cultured by a one-step method, so that the mononuclear cells are induced to immature dendritic cells. The method disclosed by the invention is simple to operate and wide in application range, can be used for efficiently inducing mononuclear cells derived from peripheral blood in different crowds, is good in activity of the induced cells, relatively high in yield of the induced cells and high in proportion of cell main groups in cytometry analysis, and has a wide application prospect.

Aspects of the present disclosure include methods for processing cytometer data, such as for group-wise analysis of the cytometer data (e.g., flow cytometry data in FCS format, mass cytometry data, genomic cytometry data). Methods according to certain embodiments include generating a compound population of events that include data accessors from cytometry data, such as where the compound population of cytometer data is from two or more different samples retained as separate raw data files (e.g., are not concatenated to form a single combined data file). Systems having an input module for receiving cytometer data and processor with memory having instructions for practicing the subject methods are also described. Non-transitory computer readable storage medium is also provided.

This disclosure provides a method for identifying and counting unlabeled (native) blood cells and analytes in whole blood. In particular, this disclosure relates to devices used within the body equipped with microfluidic cytometry optical sensors and detectors that recognize analytes or histological architecture from within a patient to provide clinically actionable data related to the subject's health.

A microfluidic impedance cytometry apparatus, for position determination and impedance measurement of particle / s in a fluid carrying particles, comprising: a microfluidic impedance flow channel for allowing flow of said fluid; an upstream section; a downstream section; a sensing region to receive said channeled fluid, to sense one or more parameters of said fluid, said sensing region comprising one or more sets of pairs of electrodes, each pair forming a current path from an operative top to an operative bottom, each of said pairs being formed by an operative top electrode and an operative bottom electrode, electric potential being applied on said operative top electrode / s, each electrode for a particular pair being parallel-aligned and being symmetric, with respect to each other, same positive electric potential being applied on each of said top electrodes and each of said bottom electrodes is virtually grounded, for a pair; and a configuration of amplifiers.