53results about How to "High throughput analysis" patented technology

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

Methods of detecting affinity interactions between at least two molecules of interest are provided. The method comprises: a. forming a plurality of interactors by coupling each molecule of interest with at least one nucleic acid moiety comprising an identification sequence element and at an association element; b. promoting an association between at least two nucleic acid moieties from different interactors to form a plurality of unique associated oligonucleotides, wherein each nucleic acid moiety may form more than one unique associated oligonucleotide, and wherein each unique associated oligonucleotide comprises at least two identification sequence elements derived from the at least two nucleic acid moieties; c. selecting the plurality of unique associated oligonucleotides; and d. subjecting the selected associated oligonucleotides to an analysis that permits detection of the at least two identification sequence elements. Similar methods directed to detecting functional interactions, libraries of interactors employable in the present methods, and kits comprising those libraries are also provided.

Transcriptomes of individual neurons provide rich information about cell types and dynamic states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation from dense adult tissue is challenging, and markers for each phase are limited. Here, Applicants developed Nuc-seq, Div-Seq, and Dronc-Seq. Div-seq combines Nuc-Seq, a scalable single nucleus RNA-Seq method, with EdU-mediated labeling of proliferating cells. Nuc-Seq can sensitively identify closely related cell types within the adult hippocampus. Div-Seq can track transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead. Finally, Applicants found rare adult newborn GABAergic neurons in the spinal cord, a non-canonical neurogenic region. Taken together, Nuc-Seq, Div-Seq and Dronc-Seq allow for unbiased analysis of any complex tissue.

The present invention relates in one aspect to a method for analyzing the frequency of interaction of a target nucleotide sequence with one or more nucleotide sequences of interest (eg. one or more genomic loci) comprising the steps of: (a) providing a sample of cross-linked DNA; (b) digesting the cross-linked DNA with a primary restriction enzyme; (c) ligating the cross-linked nucleotide sequences; (d) reversing the cross linking; (e) optionally digesting the nucleotide sequences with a secondary restriction enzyme; (f) optionally ligating one or more DNA sequences of known nucleotide composition to the available secondary restriction enzyme digestion site(s) that flank the one or more nucleotide sequences of interest; (g) amplifying the one or more nucleotide sequences of interest using at least two oligonucleotide primers, wherein each primer hybridises to the DNA sequences that flank the nucleotide sequences of interest; (h) hybridising the amplified sequence(s) to an array; and (i) determining the frequency of interaction between the DNA sequences.

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

The present invention relates in one aspect to a method for analysing the frequency of interaction of a target nucleotide sequence with one or more nucleotide sequences of interest (eg. one or more genomic loci) comprising the steps of: (a) providing a sample of cross-linked DNA; (b) digesting the cross-linked DNA with a primary restriction enzyme; (c) ligating the cross-linked nucleotide sequences; (d) reversing the cross linking; (e) optionally digesting the nucleotide sequences with a secondary restriction enzyme; (f) optionally ligating one or more DNA sequences of known nucleotide composition to the available secondary restriction enzyme digestion site(s) that flank the one or more nucleotide sequences of interest; (g) amplifying the one or more nucleotide sequences of interest using at least two oligonucleotide primers, wherein each primer hybridises to the DNA sequences that flank the nucleotide sequences of interest; (h) hybridising the amplified sequence(s) to an array; and (i) determining the frequency of interaction between the DNA sequences.

A flow cytometer includes a capillary having a sample channel; at least one vibration producing transducer coupled to the capillary, the at least one vibration producing transducer being configured to produce an acoustic signal inducing acoustic radiation pressure within the sample channel to acoustically concentrate particles flowing within a fluid sample stream in the sample channel; and an interrogation source having a violet laser and a blue laser, the violet and blue lasers being configured to interact with at least some of the acoustically concentrated particles to produce an output signal.

The present invention relates in one aspect to a method for analysing the frequency of interaction of a target nucleotide sequence with one or more nucleotide sequences of interest (eg. one or more genomic loci) comprising the steps of: (a) providing a sample of cross-linked DNA; (b) digesting the cross-linked DNA with a primary restriction enzyme; (c) ligating the cross-linked nucleotide sequences; (d) reversing the cross linking; (e) digesting the nucleotide sequences with a secondary restriction enzyme; (f) ligating one or more DNA sequences of known nucleotide composition to the available secondary restriction enzyme digestion site(s) that flank the one or more nucleotide sequences of interest; (g) amplifying the one or more nucleotide sequences of interest using at least two oligonucleotide primers, wherein each primer hybridises to the DNA sequences that flank the nucleotide sequences of interest; (h) hybridising the amplified sequence(s) to an array or sequencing the amplified sequences; and (i) determining the frequency of interaction between the DNA sequences.

The present invention relates in one aspect to a method for analyzing the frequency of interaction of a target nucleotide sequence with one or more nucleotide sequences of interest (e.g., one or more genomic loci) comprising the steps of: (a) providing a sample of cross-linked DNA; (b) digesting the cross-linked DNA with a primary restriction enzyme; (c) ligating the cross-linked nucleotide sequences; (d) reversing the cross linking; (e) digesting the nucleotide sequences with a secondary restriction enzyme; (f) ligating one or more DNA sequences of known nucleotide composition to the available secondary restriction enzyme digestion site(s) that flank the one or more nucleotide sequences of interest; (g) amplifying the one or more nucleotide sequences of interest using at least two oligonucleotide primers, wherein each primer hybridises to the DNA sequences that flank the nucleotide sequence of interest; (h) hybridising the amplified sequence(s) to an array; and (i) determining the frequency of interaction between the DNA sequences.

Method of identification and quantitative analysis of primary and / or secondary amine(s) in a sample by mass spectrometry using stable isotope labeled internal standard is provided. Said internal standard is prepared by reaction of an authentic sample of said amine with a stable isotope labeled reagent, and is added to a sample containing said amine. Said amine in said sample is then quantitatively converted to a chemical compound of identical structure, except the stable isotope atoms, as that of said internal standard using a non-labeled reagent. Said sample is then extracted and the extract is analyzed by mass spectrometry. Identification and quantification of said amine are made from a plot of ion ratio of said converted amine to said internal standard versus amine concentration.

The present invention discloses disease-linked SNPs, microRNAs, and microRNA-targeted mRNAs relevant to the pathogenesis of several major human disorders including, but not limited to, multiple types of cancers, type 2 diabetes, type 1 diabetes, Crohn's disease, coronary artery disease, hypertension, rheumatoid arthritis, bipolar disorder. Also provided are methods for the identification of disease phenotype-defining sets of SNPs, microRNAs, and mRNAs that are defined here as a “consensus disease phenocode” as well as methods of using the information provided by these consensus disease phenocodes for various diagnostic, prognostic, and / or therapeutic applications.

A method of identification and quantitative analysis of aldehydes and / or ketones in a sample by mass spectrometry using stable isotope labeled oxime internal standards or stable isotope labeled hydrazone internal standards is provided. Stable isotope labeled oxime internal standards are synthesized by reaction of an authentic sample of aldehydes and / or ketones with a stable isotope labeled alkoxylamine reagent while stable isotope labeled hydrazone internal standards are synthesized by reaction of an authentic sample of aldehydes and / or ketones with a stable isotope labeled alkylhydrazine reagent. A non labeled version of the stable isotope labeled reagent is used to convert aldehydes and / or ketones in the sample to the non labeled version of the stable isotope labeled oxime or hydrazone internal standards.

The present invention relates to protein microarray devices having an internal calibrator, kits containing such devices, and methods of using such devices. Such a device comprises a plurality of protein arraying pads on a support substrate, a plurality of protein arraying spots on each pad, and a calibrator disposed on each pad, each calibrator comprising a series of spots of increasing concentration of a protein. In preferred embodiments, each pad has an immunoglobulin calibrator that is of the same species as the proteins that are reactive to the protein arraying spots. An advantage of the present invention is that one can use reliably use microporous surfaces for conducting multiplexed protein microimmunoassays with an assurance as to surface-to-surface fluorescence variability on a microarray device.

The invention discloses a method for measuring phenolic compounds in textiles and leather products and relates a method for measuring phenolic compounds in textiles and leather products by using a high efficiency liquid chromatography-triple quadrupole mass spectrometer. According to the method disclosed by the invention, with methanol as an extracting solvent, the phenolic compounds in the textiles and leather products are extracted by adopting an ultrasonic extraction method; if interference of impurities exists, an obtained extracting solution is subjected to solid-phase extraction and purification and is subjected to volume settling by using methanol, and therefore the peak area is quantified by using qualitativeness of relative retention time and multiple reaction monitor. The method disclosed by the invention is rapid and reliable, high in flux analysis, sensitivity and recovery rate and good in precision and can be used for carrying out analysis and measurement on 10 types of phenolic compounds within 12 minutes, and the use amount of reagents is low and only 2.4ml; use of a great deal of chemical reagents is avoided, and therefore the environmental pollution is reduced; and the method is easy and feasible to measure the phenolic compounds in the textiles and leather products.

The invention belongs to the technical field of blood detection and particularly relates to a detection method and a detection system of multi-vitamins in a dry blood spot. The invention provides thedetection method of the multi-vitamins in the dry blood spot, which comprises the following steps: S101, adding an internal standard substance in the dry blood spot to obtain a treated dry blood spot;S102, extracting the treated dry blood spot with first extracting liquid to obtain first detection liquid or extracting the treated dry blood spot with second extracting liquid to obtain second detection liquid; and S103, detecting the first detection liquid or the second detection liquid by adopting a liquid chromatography-mass spectrometer, wherein the first extracting liquid is a methanol aqueous solution with a volume percentage of 5-95 percent; and the second extracting liquid is 10-90 percent of methanol aqueous solution. The detection method provided by the invention can detect the multi-vitamins in micro blood within a short time and solves the technical defects of time waste and labor waste in an existing method for detecting the multi-vitamins.

The present disclosure provides methods of assaying for antibody-dependent cell-mediated phagocytosis (ADCP). In some embodiments, the methods include monomerizing and labeling a protein, contacting the protein with a protein-specific antibody to form an antibody-protein complex, contacting the antibody-protein complex with a phagocytic cell to permit phagocytosis, and assessing the amount of internalized fluorescence.

An integrated fluorescence scanning system is provided. The integrated fluorescence scanner combines an embedded computer, light engine, microscope, and motion stage into a compact rack-mountable network appliance that allows for automation of fluorescence microscopy. In an embodiment, the integrated fluorescence scanner includes a solid-state light engine which can provide intense, pure, and stable light across the spectrum required for imaging of all fluorophores of interest. The embedded computer allows for autonomous operation, and network appliance features including synchronous multi-scanner operation and monitoring, multisite operation via a single control terminal, and calibration for inter and intra instrument consistency.

This invention relates to the field of detection of polycyclic aromatic hydrocarbons in food. The invention discloses a hydrophobic porous organic polymer solid-phase extraction material and the application of the same to the analysis of the persistent pollutant polycyclic aromatic hydrocarbons in food. The material is rich in hydrophobic benzene ring functional groups and mesoporous and microporous structures, has a large specific surface area, improves the enrichment efficiency of sample pretreatment in analysis and detection of polycyclic aromatic hydrocarbons in food and reduces matrix interference in detection of a target object. Compared with traditional solid-phase extraction materials, the material of the invention has the following advantages: the material has good physical and chemical stability; the functional groups are embedded in the skeleton of the material, and are high in content, stable and not prone to loss; and the material has long service life and can be repeatedly used.

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

The present invention relates to methods of screening for expression of a soluble candidate protein within an expression library of candidate proteins. The method involves fusing each candidate protein in the library to a peptide substrate and identifying cells that express soluble candidate protein by detecting enzymatic modification of the peptide substrate.

Methods of detecting affinity interactions between at least two molecules of interest are provided. The method comprises: a. forming a plurality of interactors by coupling each molecule of interest with at least one nucleic acid moiety comprising an identification sequence element and at an association element; b. promoting an association between at least two nucleic acid moieties from different interactors to form a plurality of unique associated oligonucleotides, wherein each nucleic acid moiety may form more than one unique associated oligonucleotide, and wherein each unique associated oligonucleotide comprises at least two identification sequence elements derived from the at least two nucleic acid moieties; c. selecting the plurality of unique associated oligonucleotides; and d. subjecting the selected associated oligonucleotides to an analysis that permits detection of the at least two identification sequence elements. Similar methods directed to detecting functional interactions, libraries of interactors employable in the present methods, and kits comprising those libraries are also provided.

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

The objects of the present invention are to provide a freeze-dried composition of an inactivated virus envelop having membrane fusion activity which can be stored at higher temperatures without losing the ability to introduce foreign matters and to provide a method of introducing a foreign matter into a cell with high efficiency.The present invention provides a freeze-dried composition for introducing a foreign matter which comprises an inactivated virus envelope having membrane fusion activity, and at least one stabilizer selected from the group consisting of a protein hydrolysate, leucine, an L-arginine-acid and a polysaccharide, and a method of introducing a foreign matter using the freeze-dried composition containing an inactivated virus envelope.

Method of identification and quantitative analysis of alcohol(s) in a sample by mass spectrometry using stable isotope labeled internal standard is provided. Said internal standard is prepared by reaction of an authentic sample of said alcohol with a stable isotope labeled reagent, and is added to a sample containing said alcohol. Said alcohol in said sample is then quantitatively converted to a chemical compound of identical structure, except the stable isotope atoms, as that of said internal standard using a non-labeled reagent. Said sample is then extracted and the extract is analyzed by mass spectrometry. Identification and quantification of said alcohol are made from a plot of ion ratio of said converted alcohol to said internal standard versus alcohol concentration.

The invention provides a micro-fluidic chip for detecting glycosylated hemoglobin and a detection method thereof. The micro-fluidic chip is sequentially provided with a cover plate layer, a fluid layer with a hollow channel structure and a substrate layer from top to bottom, and the hollow channel structure and the substrate layer jointly form a sample detection unit. A sample injection through hole and a waste liquid leading-out through hole are respectively formed in the cover plate layer. The sample detection unit comprises one or more sample injection chambers, a dilution chamber, an affinity chromatography channel, a detection chamber and a waste liquid pool. The sample injection through hole is communicated with the sample injection chamber, and the waste liquid leading-out through hole is communicated with the waste liquid pool. Sample introduction, sample dilution and optical and electrical detection are integrated through the micro-fluidic chip; the operation process is greatly simplified, the consumption of samples and reagents is reduced, expensive equipment is not needed, the use cost is largely reduced, the on-site instant detection of glycosylated hemoglobin can be realized, and huge economic value and social value are created.

The invention discloses a method for researching the action mechanism of a bactericide based on metabolomics. According to the method, pathogen hyphae respectively cultured by a bactericide-containingmedium and a bactericide-free control medium are subjected to metabolomics detection, differential metabolites are found through comparing the metabolomics research methods, common metabolites with different mechanisms of action are eliminated, a specific biomarker with a target bactericide action mechanism is acquired, and the bactericide action mechanism is identified and is subjected to high-throughput analysis through biomarker content change. The invention also discloses a use of succinic acid as a biomarker of a succinate dehydrogenase inhibitor. The method is fast and reliable and canproduce a biomarker with strong specificity. Through biomarker specificity change, the rapid identification and high-throughput analysis of the active substance action mechanism are realized.

Disclosed herein is a novel method of measuring telomere length comprising determining the DNA content (Dx) of a sample, determining the telomeric content (T) of a sample, and determining the value of T / Dx.

The invention discloses a method for determining nitrite in food by employing a headspace-gas chromatography / mass spectrography. The method comprises the following steps: (1) carrying out ultrasonic extraction on to-be-tested food; (2) adding zinc acetate, a potassium ferrocyanide solution and purified water to a solution which is extracted from the step (1), precipitating protein and filtering; (3) enabling filtrate obtained from the step (2) to act on sodium cyclamate in an acidic condition, putting into a headspace bottle, determining and analyzing by adopting the gas chromatography and mass spectrography; and (4) drawing a calibration curve according to the content of the nitrite corresponding to response peak area of a derived product cyclohexene, and carrying out quantitative analysis. According to the method for detecting the nitrite in the food, the quantitative detection limit is 100miccrog / kg; the sodium nitrite standard is below 0.1mg / L to 5mg / L; the response value and the mass concentration are in a good linear relationship; the related coefficient is greater than 0.998; the recovery rate of the nitrite in a tested article is 85.3%-102%; and the relative standard deviation (RSD) is less than 5.0%.