53results about How to "High throughput analysis" patented technology

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

Methods of detecting affinity interactions between at least two molecules of interest are provided. The method comprises: a. forming a plurality of interactors by coupling each molecule of interest with at least one nucleic acid moiety comprising an identification sequence element and at an association element; b. promoting an association between at least two nucleic acid moieties from different interactors to form a plurality of unique associated oligonucleotides, wherein each nucleic acid moiety may form more than one unique associated oligonucleotide, and wherein each unique associated oligonucleotide comprises at least two identification sequence elements derived from the at least two nucleic acid moieties; c. selecting the plurality of unique associated oligonucleotides; and d. subjecting the selected associated oligonucleotides to an analysis that permits detection of the at least two identification sequence elements. Similar methods directed to detecting functional interactions, libraries of interactors employable in the present methods, and kits comprising those libraries are also provided.

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

The present invention relates in one aspect to a method for analysing the frequency of interaction of a target nucleotide sequence with one or more nucleotide sequences of interest (eg. one or more genomic loci) comprising the steps of: (a) providing a sample of cross-linked DNA; (b) digesting the cross-linked DNA with a primary restriction enzyme; (c) ligating the cross-linked nucleotide sequences; (d) reversing the cross linking; (e) optionally digesting the nucleotide sequences with a secondary restriction enzyme; (f) optionally ligating one or more DNA sequences of known nucleotide composition to the available secondary restriction enzyme digestion site(s) that flank the one or more nucleotide sequences of interest; (g) amplifying the one or more nucleotide sequences of interest using at least two oligonucleotide primers, wherein each primer hybridises to the DNA sequences that flank the nucleotide sequences of interest; (h) hybridising the amplified sequence(s) to an array; and (i) determining the frequency of interaction between the DNA sequences.

The present invention discloses disease-linked SNPs, microRNAs, and microRNA-targeted mRNAs relevant to the pathogenesis of several major human disorders including, but not limited to, multiple types of cancers, type 2 diabetes, type 1 diabetes, Crohn's disease, coronary artery disease, hypertension, rheumatoid arthritis, bipolar disorder. Also provided are methods for the identification of disease phenotype-defining sets of SNPs, microRNAs, and mRNAs that are defined here as a “consensus disease phenocode” as well as methods of using the information provided by these consensus disease phenocodes for various diagnostic, prognostic, and/or therapeutic applications.

The invention discloses a method for measuring phenolic compounds in textiles and leather products and relates a method for measuring phenolic compounds in textiles and leather products by using a high efficiency liquid chromatography-triple quadrupole mass spectrometer. According to the method disclosed by the invention, with methanol as an extracting solvent, the phenolic compounds in the textiles and leather products are extracted by adopting an ultrasonic extraction method; if interference of impurities exists, an obtained extracting solution is subjected to solid-phase extraction and purification and is subjected to volume settling by using methanol, and therefore the peak area is quantified by using qualitativeness of relative retention time and multiple reaction monitor. The method disclosed by the invention is rapid and reliable, high in flux analysis, sensitivity and recovery rate and good in precision and can be used for carrying out analysis and measurement on 10 types of phenolic compounds within 12 minutes, and the use amount of reagents is low and only 2.4ml; use of a great deal of chemical reagents is avoided, and therefore the environmental pollution is reduced; and the method is easy and feasible to measure the phenolic compounds in the textiles and leather products.

The invention belongs to the technical field of blood detection and particularly relates to a detection method and a detection system of multi-vitamins in a dry blood spot. The invention provides thedetection method of the multi-vitamins in the dry blood spot, which comprises the following steps: S101, adding an internal standard substance in the dry blood spot to obtain a treated dry blood spot;S102, extracting the treated dry blood spot with first extracting liquid to obtain first detection liquid or extracting the treated dry blood spot with second extracting liquid to obtain second detection liquid; and S103, detecting the first detection liquid or the second detection liquid by adopting a liquid chromatography-mass spectrometer, wherein the first extracting liquid is a methanol aqueous solution with a volume percentage of 5-95 percent; and the second extracting liquid is 10-90 percent of methanol aqueous solution. The detection method provided by the invention can detect the multi-vitamins in micro blood within a short time and solves the technical defects of time waste and labor waste in an existing method for detecting the multi-vitamins.

An integrated fluorescence scanning system is provided. The integrated fluorescence scanner combines an embedded computer, light engine, microscope, and motion stage into a compact rack-mountable network appliance that allows for automation of fluorescence microscopy. In an embodiment, the integrated fluorescence scanner includes a solid-state light engine which can provide intense, pure, and stable light across the spectrum required for imaging of all fluorophores of interest. The embedded computer allows for autonomous operation, and network appliance features including synchronous multi-scanner operation and monitoring, multisite operation via a single control terminal, and calibration for inter and intra instrument consistency.

This invention relates to the field of detection of polycyclic aromatic hydrocarbons in food. The invention discloses a hydrophobic porous organic polymer solid-phase extraction material and the application of the same to the analysis of the persistent pollutant polycyclic aromatic hydrocarbons in food. The material is rich in hydrophobic benzene ring functional groups and mesoporous and microporous structures, has a large specific surface area, improves the enrichment efficiency of sample pretreatment in analysis and detection of polycyclic aromatic hydrocarbons in food and reduces matrix interference in detection of a target object. Compared with traditional solid-phase extraction materials, the material of the invention has the following advantages: the material has good physical and chemical stability; the functional groups are embedded in the skeleton of the material, and are high in content, stable and not prone to loss; and the material has long service life and can be repeatedly used.

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

The invention provides a micro-fluidic chip for detecting glycosylated hemoglobin and a detection method thereof. The micro-fluidic chip is sequentially provided with a cover plate layer, a fluid layer with a hollow channel structure and a substrate layer from top to bottom, and the hollow channel structure and the substrate layer jointly form a sample detection unit. A sample injection through hole and a waste liquid leading-out through hole are respectively formed in the cover plate layer. The sample detection unit comprises one or more sample injection chambers, a dilution chamber, an affinity chromatography channel, a detection chamber and a waste liquid pool. The sample injection through hole is communicated with the sample injection chamber, and the waste liquid leading-out through hole is communicated with the waste liquid pool. Sample introduction, sample dilution and optical and electrical detection are integrated through the micro-fluidic chip; the operation process is greatly simplified, the consumption of samples and reagents is reduced, expensive equipment is not needed, the use cost is largely reduced, the on-site instant detection of glycosylated hemoglobin can be realized, and huge economic value and social value are created.

Disclosed herein is a novel method of measuring telomere length comprising determining the DNA content (Dx) of a sample, determining the telomeric content (T) of a sample, and determining the value of T/Dx.

The invention discloses a method for determining nitrite in food by employing a headspace-gas chromatography/mass spectrography. The method comprises the following steps: (1) carrying out ultrasonic extraction on to-be-tested food; (2) adding zinc acetate, a potassium ferrocyanide solution and purified water to a solution which is extracted from the step (1), precipitating protein and filtering; (3) enabling filtrate obtained from the step (2) to act on sodium cyclamate in an acidic condition, putting into a headspace bottle, determining and analyzing by adopting the gas chromatography and mass spectrography; and (4) drawing a calibration curve according to the content of the nitrite corresponding to response peak area of a derived product cyclohexene, and carrying out quantitative analysis. According to the method for detecting the nitrite in the food, the quantitative detection limit is 100miccrog/kg; the sodium nitrite standard is below 0.1mg/L to 5mg/L; the response value and the mass concentration are in a good linear relationship; the related coefficient is greater than 0.998; the recovery rate of the nitrite in a tested article is 85.3%-102%; and the relative standard deviation (RSD) is less than 5.0%.