Micro-fluidic chip for detecting glycosylated hemoglobin and detection method thereof

A glycosylated hemoglobin, microfluidic chip technology, applied in chemical instruments and methods, laboratory containers, biological tests, etc., can solve the problems of complicated operation procedures, high instrument cost, unfavorable diabetes application and promotion, etc., to simplify Operational flow, simple operation, and the effect of shortened sample processing time

Active Publication Date: 2020-10-16
上海佰芯生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among the above methods, although high-performance liquid ion chromatography (HPLC) is recognized as the gold standard method, it has disadvantages such as complicated operating procedures and high instrument costs, and often requires a laboratory-level operating environment and professional technical operators. , so this method is not conducive to the application and promotion of instant and rapid detection of diabetes

Method used

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  • Micro-fluidic chip for detecting glycosylated hemoglobin and detection method thereof
  • Micro-fluidic chip for detecting glycosylated hemoglobin and detection method thereof
  • Micro-fluidic chip for detecting glycosylated hemoglobin and detection method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0033] refer to figure 1 and figure 2 As shown, a microfluidic chip for detecting glycosylated hemoglobin provided by the embodiment of the present invention, the microfluidic chip is provided with a cover sheet layer 1 and a fluid layer 2 with a hollow channel structure in sequence from top to bottom and the base layer 3 , the hollow channel structure and the base layer jointly constitute a sample detection unit.

[0034] The cover layer is respectively provided with a sampling through hole 11 for sampling in the sampling chamber and a waste liquid outlet through hole 12 for draining out the waste liquid from the waste liquid pool.

[0035] The sample detection unit includes one or more sample injection chambers 21, a dilution chamber 22, an affinity chromatography channel 23, a detection chamber 24 and a waste liquid pool 25, and the sample injection through hole 11 communicates with In the sampling chamber 21 , the waste liquid outlet through hole 12 communicates with th...

Embodiment 2

[0060] refer to image 3 As shown, a microfluidic chip for detecting glycosylated hemoglobin provided in this example differs from Example 1 in that:

[0061] The sample detection unit includes two sample injection chambers 21 , and the two sample injection chambers 21 are respectively connected to the dilution chamber 22 for introducing different samples into the dilution chamber 22 . When in use, the whole blood sample is introduced from the first sampling chamber 211 , and in step S2 , the diluent is injected from the second sampling chamber 212 and detected according to the detection steps in Example 1.

[0062] The microfluidic chip for detecting glycosylated hemoglobin provided in this embodiment only needs to sequentially inject whole blood, diluent and mobile phase from the sample injection chamber 21 during use, which can realize fully automatic detection, and it can be completed within 3 to 10 minutes. Able to detect glycosylated hemoglobin. This embodiment has the...

Embodiment 3

[0064] refer to Figure 4 As shown, a microfluidic chip for detecting glycosylated hemoglobin is provided in this example, the difference from Example 1 is:

[0065] The microfluidic chip of this embodiment includes three sample detection units. During detection, three different whole blood samples are added to the corresponding sampling chamber 21 respectively, and the detection is carried out according to the detection steps in Example 1, and the detection results of glycated hemoglobin of the three samples can be obtained within 10 minutes. This embodiment has the advantages of convenient sample injection, less sample consumption, high reaction efficiency, and short detection time.

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Abstract

The invention provides a micro-fluidic chip for detecting glycosylated hemoglobin and a detection method thereof. The micro-fluidic chip is sequentially provided with a cover plate layer, a fluid layer with a hollow channel structure and a substrate layer from top to bottom, and the hollow channel structure and the substrate layer jointly form a sample detection unit. A sample injection through hole and a waste liquid leading-out through hole are respectively formed in the cover plate layer. The sample detection unit comprises one or more sample injection chambers, a dilution chamber, an affinity chromatography channel, a detection chamber and a waste liquid pool. The sample injection through hole is communicated with the sample injection chamber, and the waste liquid leading-out through hole is communicated with the waste liquid pool. Sample introduction, sample dilution and optical and electrical detection are integrated through the micro-fluidic chip; the operation process is greatly simplified, the consumption of samples and reagents is reduced, expensive equipment is not needed, the use cost is largely reduced, the on-site instant detection of glycosylated hemoglobin can be realized, and huge economic value and social value are created.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, and in particular to a microfluidic chip for detecting glycosylated hemoglobin and a detection method thereof. Background technique [0002] Diabetes mellitus is a metabolic disease characterized by hyperglycemia due to defects in insulin secretion or insulin action. At present, diabetes is mainly judged by the detection of blood sugar parameters, but blood sugar parameters can only represent the blood sugar level at the time of blood drawing, which has limitations in the diagnosis of diabetes. Medical research in recent years has proved that the concentration of glycosylated hemoglobin (GHb) in the blood is relatively stable, and its concentration can accurately reflect the blood sugar level in the last 2 to 3 months, which is convenient for doctors to diagnose diabetes early and understand the control of diabetes. [0003] At present, there are two main types of methods for detectin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/27G01N27/26G01N33/72G01N35/00
CPCB01L3/5027G01N35/00G01N35/00029G01N21/27G01N27/26G01N33/723G01N33/726G01N2035/00158B01L2200/0631B01L2300/0829B01L2300/0861B01L2300/0887Y02A50/30
Inventor 罗云和
Owner 上海佰芯生物科技有限公司
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