447results about How to "Reduce cross contamination" patented technology

Methods and systems for performing single molecule amplification for detection, quantification and statistical analysis of nucleic acids are provided. Methods and systems are provided for determining and quantifying lengths of nucleic acids of interest.

The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method.

The present invention relates to a capsule containing a substance for the preparation of a beverage that is designed to be extracted by injection of a fluid under pressure. The capsule comprises a closed chamber containing the substance with at least part of the closed chamber including a retaining part made of a flexible membrane. One or more raised elements that face the membrane are associated with and forming part of the capsule. These element(s) are used to open the capsule due to a buildup of pressure therein. This rise in pressure causes relative engagement of the raised element(s) with the membrane until the membrane ruptures or is torn to release the formed beverage from the chamber. As the liquid of the beverage does not come into contact with the machine, hygiene is improved and cross-contamination is reduced when preparing a beverage from the capsule.

The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

A method and apparatus enables linered label applicators to use labels on thin liners. Linered labels comprise a composite of an elongate sheet of thin or light-weight temporary liner having a precut label adhered to a low adhesion surface. The cut-out labels on the liner are fed into the linered label applicator. The use of support mechanisms other then vacuum application on rollers enables the use of thinner liner sheets on labels. A die head for cutting or perforating labels comprising adhesive on label stock comprising a die head having at least 80% of its outer surface comprising flat areas between cutting edges, multiple cutting edges on the outer surface, and an internal volume in the die head, the internal volume for carrying coolant liquid into and out of the volume so as to cool the outer surface of the die head when the coolant temperature is at least 10° C. cooler than the outer surface of the die head.

A Fe-matched matter-halogen electrochemical system used on liquid flow energy storage is prepared as separating positive/negative electrode electrolyte by positive ion exchange film and storing said electrolyte in external container, generating charge/discharge process by making oxidation/reduction reaction on inert carbon felt electrode through pump stream via battery, applying soluble Fe(III)/Fe(II) -matched matter oxidation/reduction electric pair as negative electrode and applying halogen oxidation/reduction electric pair as positive electrode.

The invention discloses a kit for extracting nucleic acid from bacteria by using a paramagnetic particle method and an extracting method. The kit comprises a bacteria lysate, a magnetic bead binding solution, a magnetic bead scrubbing solution and a nucleic acid eluent. The bacteria lysate comprises sodium dodecyl sulfate, ethylenediaminetetraacetic acid, tri-hydroxymethyl aminomethane and sodium chloride; the magnetic bead binding solution comprises polyethylene glycol-8000 and sodium chloride; the magnetic bead scrubbing solution comprises ethanol; and the nucleic acid eluent comprises tri-hydroxymethyl aminomethane and ethylenediamine tetraacetic acid. The kit comprises the unique bacteria lysate which has a strong lysis function for the bacteria; and the kit can be used for manual extraction and instrument extraction by using a commercially available nucleic acid isolation machine, high-purity and high-concentration bacteria nucleic acid can be extracted.

Embodiments of the present invention provide apparatus and methods for loading and unloading a multiple-substrate processing chamber segment by segment. One embodiment of the present invention provides an apparatus for processing multiple substrates. The apparatus includes a substrate supporting tray having a plurality of substrate pockets forming a plurality of segments, and a substrate handling assembly configured to pick up and drop off substrates from and to a segment of substrate pockets of the substrate supporting tray.

A multi-channel flow cell can allow for reduced cross-contamination in sample loading and the ability to observe activity within the flow cell once the channels are loaded. A multi-channel flow cell includes a plurality of independently-addressable channels sandwiched between a two substrates. Each of the channels can be coated with a layer that facilitates support-binding of an analyte. Each of the channels terminates on one end in an inlet and on the other end in an outlet. A loading block having inlet ports that match the inlets of the channels can be mated to the inlets of the channels, and an outlet block can be mated to the outlets of the channels. Analytes can be introduced into the channels via the inlet ports of the loading block and are pulled through the channels by capillary action or by vacuum. Once analyte has been introduced into each of the channels, the loading and outlet blocks can be removed and the device turned over. Such a flow cell can be used for streamlining the process of reaction and interrogation of biochemical assays at the microfluidic level. Reagents can be introduced into each of the channels of the flow cell for chemical reactions therein, excess reagent being washed out through the channel outlets. Observation of optically-detectable moieties is then conducted. With such a flow cell optical labels associated with incorporation in a sequencing-by-synthesis reaction can be observed.

Systems for differentiating the lengths of nucleic acids of interest in a sample are provided. The system includes a microfluidic device, a detector, and a software system. The microfluidic device includes an amplification microchannel or microchamber containing a reaction mixture under conditions that provide one or more amplicons of the nucleic acid of interest. The detector is integral with or proximal to the microfluidic device and is configured to detect the amplicons as one or more signals from a homogenous mixture. The software system interprets one or more coincidentally detected signals to indicate lengths of one or more individual nucleic acid molecules of interest, thereby differentiating the lengths of the nucleic acids of interest.

The invention relates to an EML4-ALK (echinoderm microtubule associated protein like4-anaplastic lymphoma kinase) fusion gene fluorescent quantitative PCR (polymerase chain reaction) assay kit, which is applicable to assay of EML4-ALK fusion gene mutation in lung adenocarcinoma. The kit comprises probes, primers and positive controls, which are specially designed for conserved sequences of 9 fusion variations of the EML4-ALK fusion gene. The kit can be used for quickly and accurately assaying 9 most common EML4-ALK fusion gene variations with high sensitivity, namely 9 fusion variations of 7 variant subtypes, which are subtype 1 (E13; A20), subtype 2 (E20; A20), subtype 3 (E6a/b; A20), subtype 4 (E14; A20), subtype 5 (E2a/b; A20), subtype 6 (E18; A20) and subtype 7 (E14; A20), so that a real-time fluorescent quantitative PCR system for assaying 9 most common EML4-ALK fusion gene variations can be established.

A detector for underwater electromagnetic surveying is described. The detector comprises first, second, third and fourth electrodes which are arranged to define first, second and third electric dipole antennae extending between pairs of the electrodes. Each dipole antennae extends between a pair of the electrodes and the fourth electrode is common to all three dipole antennae. Thus the first electrode is separated from the fourth electrode along a first direction to provide the first dipole antenna, the second electrode is separated from the fourth electrode along a second direction to provide the second dipole antenna, and the third electrode is separated from the fourth electrode along a third direction to provide the third dipole antenna. The electrodes are arranged so that the first, second and third directions are inclined at an angle of between 20 and 70 degrees to a surface on which the detector rests when in normal use.

An automatic biochemical analyzing method is described, in which a row of reaction containers is cyclically arrayed such that an operating cycle is formed that is defined between dispensing the preceding sample and the sequent sample. In each cycle, the reaction containers are transferred so as to motivate at least one reaction container to pass across an optical detecting channel and cause the reaction containers to make an intermediate pause when no sample is dispensed. When the reaction containers pause, the sample is dispensed to a reaction container at a sample dispensing position. When the reaction containers are at the intermediate pause, the first reagent is dispensed to a reaction container at a first reagent dispensing position. In each reaction container, the operation cycle when the sample is dispensed follows the operation cycle when the first reagent is dispensed. An automatic biochemical analyzing apparatus is also disclosed.

The invention discloses a mixed type creep feed which is prepared by mixing granule and powder, wherein the weight ratio of the granule to the powder is 65-70:35-30. The mixed type creep feed of the invention selects high quality raw materials and simulates breast milk, wherein the powder is mainly based on the nutritional requirements of weak and small piglets and the granule mainly considers the nutritional requirements of normal piglets. The mixed type creep feed of the invention is suitable for piglets of 7 days old-10 days after weaning. The invention realizes reductions of cross contamination and environment pollution in the production process, highly automatic control, and reduction of energy consumption by improving the feed conversion ratio. The overall system process improves the quality of early weaning feed for the piglets, simultaneously guarantees food safety, realizes high automation, simultaneously maintains environment protection and reduces energy consumption.

The invention discloses a capillary needle. The capillary needle comprises a tube body, the two ends of the tube body are respectively provided with an inlet and an outlet for electro-spray, a part of the tube body is bent to form a sampling end, and the sampling end is provided with a sampling port communicating with a cavity in the tube body. The invention also discloses an electro-spray ionization mass spectrometry analytical apparatus applying the capillary needle. The invention also simultaneously discloses a method applying the electro-spray ionization mass spectrometry analytical apparatus for analytical detection. The analytical apparatus is high in integration, compact in structure, easy to control, small in size and low in cost; the sample consumption is greatly reduced, and the experiment cost is decreased; an integrated sample introduction method is employed, and an sample introduction end does not have to be repeatedly placed in a sample and blank solution, such that cross contamination is reduced, and more importantly, the analytical flux is substantially improved; and through combination with an automatic sample changing system, different samples can be automatically introduced, and therefore, the capillary needle, the apparatus and the method are quite suitable for such fields as high-flux screening, unicell analysis, mass spectrum imaging analysis and the like.

A weir assembly for use with a mixer-settler for liquid-liquid extraction includes in one embodiment an organic weir having an inlet opening below the free surface of the organic phase liquid and a vertically-adjustable front wall allowing adjustments in the position and/or height of the inlet opening. The organic weir can also feature an angled bottom coupled to a lip segment, an incline plate in the interior of the weir, and a front wall that is angled with respect to incoming fluid flow. In another embodiment the weir assembly can independently or additionally include an aqueous weir with a labyrinth section. The aqueous weir can optionally include an adjustable lip hingedly coupled to the top of the final partition in the labyrinth section.

The invention discloses a biochip spotting platform which relates to the preparation of a microarray biochip. The biochip spotting platform mainly utilizes a precision linear motor which can carry out the high-speed motion for constituting a three-dimensional distribution mechanical hand, and simultaneously comprises a spotting head with 1 to 48 spotting needles, a chip fixed platform, a sample box platform and a spotting needle cleaning and drying device are arranged on a working platform with the water bath cooling function, an electric control box and the distribution mechanical hand are operated under the control of software of a computer, thereby rapidly completing the spotting and finally forming the complete biochip. The spotting platform is characterized by compact structure, simple and convenient operation, high speed, efficiency and precision, less cross-infection and broad applicability, etc., thereby being very applicable to the scale production of the biochips and also being applicable to scientific research and applications.

A method and system for analysis of additives in electrolysis plating solutions, using a flow management system that minimizes loss of plating solutions and decreases sampling time. The system includes at least one analysis chamber, a sampling duct connected to processing tool, a four-way valve positioned between the processing tool and the sampling duct, at least one carrier fluid duct connected to the analysis chamber, at least one actuatable multi-port valve that provides a transference platform between the sampling duct and the at least one carrier fluid duct, and a flow sensor connected to the sampling duct and positioned downstream from the at least one actuatable multi-port valve.

A microfluidic device having a delay structure and a sample testing apparatus including the microfluidic device are provided. The microfluidic device includes: a reaction chamber which contains a reagent capable of reacting with a sample; a distribution channel through which the sample is provided to the reaction chamber; an inlet channel through which the at least one reaction chamber is connected with the distribution channel; and a delay structure which is located between the at least one reaction chamber and the distribution channel, and delays movement of contents of the reaction chamber to the distribution channel.