542 results about "Breast milk" patented technology

The invention belongs to the technical field of biology, and provides a porcine epidemic diarrhea virus mRNA vaccine and application. The mRNA molecule comprises a coding region for coding the S protein of the PEDV; the PEDV is of a G2a type, and two proline mutations I1076P and L1077P are introduced into an S protein. The invention further discloses a DNA molecule. An S protein of PEDV; a recombinant plasmid; recombining the host cell; an mRNA vaccine formulation which can be used to prepare an antibody against the S protein of PEDV after immunizing an animal; the invention further discloses application of the mRNA molecule, the DNA molecule, the recombinant plasmid or the host cell to preparation of a medicine for preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The vaccine prepared by the invention is fast in antibody generation and high in protection rate, can generate high-level neutralizing antibodies in serum and breast milk of sows after the pregnant sows are immunized before parturition, has a remarkable protection effect on piglets, and can effectively prevent the piglets from being infected with PEDV (Porcine Epidemic Diarrhea Virus).

The present invention relates to a nutritional composition, food and pharmaceutical products comprising the nutritional composition, and uses thereof. The nutritional composition comprises medium and long chain fatty acid triglycerides and breast milk oligosaccharides, and the weight ratio of the medium and long chain fatty acid triglycerides to the breast milk oligosaccharides is 1: 1 to 9: 1. The nutritional composition disclosed by the invention can play a role in synergistically promoting skeletal development and particularly improving bone mineral density.

The invention belongs to the field of food, and particularly relates to a nutritional composition containing probiotics and breast milk oligosaccharide for improving anxiety or depression and application of the nutritional composition. The invention provides application of a nutritional composition in preparation of food beneficial to improving constipation and anxiety state and / or depression state caused by constipation. Wherein the nutritional composition comprises the following components (i) and (ii): (i) a bifidobacterium probiotic, and (ii) a sialylated breast milk oligosaccharide; the bifidobacterium probiotics at least comprise animal bifidobacterium subsp. Lactis, the sialylated breast milk oligosaccharide at least comprises 6 '-sialylated lactose, and the animal bifidobacterium subsp. Lactis comprises a Bb-12 strain. The substance combination of the bifidobacterium probiotics and the sialylated breast milk oligosaccharide can synergistically improve abnormal brain-intestine interaction, and improve constipation and anxiety and / or depression state caused by constipation.

A breast milk-derived Limosilactobacillus fermentum MSJK0025 capable of improving the intestinal flora condition and the use thereof. The breast milk-derived Limosilactobacillus fermentum MSJK0025 is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No. 26426. The breast milk-derived Limosilactobacillus fermentum is a new strain isolated from breast milk of healthy puerperae, and is referred to as Limosilactobacillus fermentum MSJK0025. The strain has a good antibiotic sensitivity and stable inheritance of traits, has a good tolerance to acid, bile salts and artificial gastric / intestinal fluid, and has a fast growth rate and good safety. Furthermore, the strain has a strong intestinal colonization capability and can effectively inhibit intestinal pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Candida albicans, but has no inhibitory effects on intestinal probiotics such as Lactobacillus and Bifidobacterium.

The invention provides a breast milk source exosome as well as a preparation method and application thereof. The preparation method of the breast milk source exosome comprises the following steps: pre-treating breast milk, and then carrying out primary purification concentration and fine purification on the breast milk source exosome after the pre-treatment; wherein the pretreatment comprises the step of mixing degreased breast milk with a sodium phosphate solution; the primary purification concentration comprises tangential flow ultrafiltration treatment; the fine purification comprises purification treatment by adopting a multi-mode chromatography. The invention provides a large-scale purification process of the breast milk exosome, which can be used for treating a large number of samples, improving the purity and yield of the breast milk exosome and ensuring the morphological integrity of the breast milk exosome.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum subsp. Longum as well as a composition, a pharmaceutical composition, a culture, a food product or a dietary supplement containing the bifidobacterium longum subsp. Longum and application of the bifidobacterium longum subsp. Longum. The bifidobacterium longum is preserved in the Guangdong Microbial Culture Collection Center on January 21, 2025, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.65836. The bifidobacterium longum subsp. Longum has relatively strong gastric acid and cholate tolerance and intestinal colonization capability; the bifidobacterium longum subsp. Longum can effectively utilize bifidobacterium bifidum to release monosaccharide or disaccharide outside cells for efficient proliferation, and the composition containing the bifidobacterium longum subsp. Longum, the bifidobacterium bifidum and breast milk oligosaccharide can release a large amount of short-chain fatty acid, so that the effects of maintaining the physiological function of an organism and improving the health of the organism are achieved.

The invention relates to a bifidobacterium longum subsp. Infantis Imu-01 and an application thereof. Specifically, the invention provides bifidobacterium longum subsp.infantis Imu-01, a culture, a preparation and application of the bifidobacterium longum subsp.infantis Imu-01 in preparation of a product for improving the immune function. The bifidobacterium longum subsp. Infantis provided by the invention can regulate and control the immune level of an object, improve the content of butyric acid in intestinal tracts and improve the utilization of oligosaccharide in breast milk.

The invention provides a breast milk feeding amount intelligent calculation and dynamic adjustment method and system, and relates to the technical field of intelligent feeding, and the method comprises the steps: collecting real-time physiological parameters of an infant, constructing an infant digital model through a multi-scale morphological neural network, calculating a basic energy consumption value through combining with a biological rhythm prediction model, and calculating the feeding amount of the infant. The standard breast milk feeding amount is calculated through the self-adaptive fuzzy neural network; the method comprises the following steps: collecting mother physiological state data and breast milk component data, calculating a breast milk nutrition density coefficient by using a deep mixing cognitive network and an immune evolution self-organizing network, correcting a breast milk reference feeding amount, and finally calculating an actual breast milk feeding amount through a self-adaptive resonance theory network; growth and development data of an infant after breast milk intake are collected, a multi-layer spiral pulse neural network is used for extracting development feature vectors, a development deviation value is calculated based on a fractal dynamics model, network parameters are dynamically adjusted through a biological group intelligent optimization system, and dynamic optimization of breast milk feeding amount is achieved.

The invention belongs to the technical field of nutritious food, and discloses a composition for simulating whole breast milk oligosaccharide and application of the composition. In the composition for simulating the whole breast milk oligosaccharide, the mass ratio of 2 '-fucosyllactose to lactose-N-neotetraose to 3'-sialyllactose is (3.9-8.1): (0.8-1.2): (0.8-1.2), and the breast milk oligosaccharide 2 '-fucosyllactose, the lactose-N-neotetraose and the 3'-sialyllactose are mutually matched to take effect in a synergistic manner, so that the whole breast milk oligosaccharide is obtained. The composition can improve the memory ability and cognitive ability of an eating object, can be added into infant food (including infant formula powder, complementary food and nutritional supplements) and nutritional supplements or food of children over 3 years old, teenagers and aged people, and has a wide application prospect.

The invention belongs to the technical field of food, and relates to application of breast milk oligosaccharide in regulating brain immunity. Specifically, the invention relates to the non-therapeutic purpose of adjusting brain immunity by adjusting polarization of microglial cells M1 / M2, and the breast milk oligosaccharide is any one of 3-fucosyllactose, lactose-N-tetraose, lactose-N-neotetraose, 3 '-sialic acid lactose and 6'-sialic acid lactose. Research results show that a specific variety of breast milk oligosaccharides (especially 3-FL) can obviously inhibit the generation of neurotoxic factors NO, inflammatory factors TNF-alpha and IL-1beta in microglial cells, and promote the generation of anti-inflammatory cytokines IL-10 in the microglial cells, so that the state of the microglial cells is improved, damage repair is promoted, and the microglial cells are prevented from being damaged. Therefore, the effect of regulating brain immunity is achieved.

The invention provides a milk powder composition containing breast milk oligosaccharide and easy to digest and absorb. The milk powder composition comprises the breast milk oligosaccharide, OPN, galactooligosaccharide, lactoferrin, alpha-whey protein, multivitamins, lactose, vegetable oil and compound minerals, wherein the weight ratio of the lactoferrin to the OPN to the breast milk oligosaccharide to the galactooligosaccharide is 1: (2-4): (2-50): (4-100). The composition can effectively recover and improve the intestinal barrier function caused by inflammatory factors, can be well absorbed by infants with poor digestion and absorption functions, reduces diarrhea and promotes skeletal development.

The invention belongs to the field of food, and particularly relates to a nutritional composition of oligosaccharide and probiotics and application of the nutritional composition. The invention provides application of a nutritional composition in preparation of food beneficial to improvement of constipation and improvement of cognitive competence decline caused by constipation. Wherein the nutritional composition comprises the following components (i) and (ii): (i) a neutral fucosylated breast milk oligosaccharide, and (ii) a bifidobacterium probiotic; wherein the neutral fucosylated breast milk oligosaccharide at least comprises 2 '-fucosylated lactose, and the bifidobacterium probiotics at least comprise bifidobacterium longum subsp. Longum. According to the present invention, with the substance combination of the neutral fucosylated breast milk oligosaccharide and the bifidobacterium probiotic, the abnormal brain-intestine interaction can be improved, such that the constipation can be easily improved, and the cognitive ability reduction caused by the constipation can be easily improved;

The present application relates to a kind of Bifidobacterium longum infantis subsp.Imu-01 and its application. Specifically, the present application provides Bifidobacterium longum infantis subsp.Imu-01, culture, preparation and preparation of immune function improving product application. The present application provides that Bifidobacterium longum infantis can regulate the immune level of the subject, improve the content of intestinal butyric acid, improve the utilization of human milk oligosaccharide.

The invention relates to recombinant escherichia coli and a method for synthesizing sialic acid lactose-N-tetrasaccharide c, and belongs to the technical field of microbial metabolism engineering. According to the research, engineering escherichia coli BAZ for producing LNTri II is taken as an initial strain, two key glycosyl transferases, namely beta 1, 4-galactosyl transferase and alpha 2, 6-sialyltransferase, and three CMP-Neu5Ac pathway enzymes, namely NeuC, NeuB and NeuA, are introduced, and related competitive genes are knocked out; under the conditions of shake-flask culture and fed batch culture, the LST c yield of 1.718 g / L and the LST c yield of 9.745 g / L are achieved through the engineering bacterium respectively, and the huge potential of a microbial cell factory in the aspect of efficient production of complex breast milk oligosaccharides is shown.

The present invention belongs to the technical field of breast milk secretion detection, and provides a wearable device for dynamically detecting the amount of milk secretion. The present invention includes a multimodal measurement device and a control unit. The multimodal measurement device includes at least two types of electrodes, and the electrodes include an excitation electrode close to the root of the breast and a measuring electrode close to the nipple. The multimodal measurement device also includes a micro-flow measurement mechanism, and at least two fluid channels are provided inside the micro-flow measurement mechanism. One end of the fluid channel is connected to the side of the wearable device close to the nipple, and the other end is connected to the outside of the wearable device. To ensure the uniformity of the current covering the breast, the measuring electrode close to the nipple is close to the measurement target tissue, which directly reflects the impedance changes of the lactation-related tissue; the micro-flow measurement mechanism is used to provide physical parameters independent of the impedance measurement, which can provide a reference standard for the lactation amount measured by impedance, and improve the robustness and accuracy of the lactation measurement system.

The invention discloses a method for rapidly determining the content of breast milk oligosaccharide in dairy products, which comprises the following steps: sampling and pretreating different types of dairy products according to sample states, carrying out ultrasonic extraction, refrigerated centrifugal degreasing and centrifugal ultrafiltration protein purification on the obtained sample, and reducing the purified sample solution by using sodium borohydride to obtain a sample solution to be detected; reducing the breast milk oligosaccharide standard substance by sodium borohydride to obtain a standard solution to be detected; determining the standard substance solution and the to-be-detected sample solution by adopting ultra-high performance liquid chromatography-tandem mass spectrometry, establishing a standard curve, and carrying out quantitative analysis on the to-be-detected sample solution; the chromatographic conditions are as follows: an ACQUITY UPLC BEH Amide hydrophilic interaction chromatographic column is adopted; the mobile phase A is 20 mmol / L ammonium formate; the mobile phase B is acetonitrile; and gradient elution.

A method and application of Escherichia coli BL21star (DE3) BLBRTXST3 and its fermentation method for producing high-purity lactose-N-neotetraose, the invention relates to the technical field of human milk oligosaccharide production, Escherichia coli )BL21 star (DE3) BLBRTXST3 is deposited in the General Microbiology Center of China Microorganism Culture Collection Committee, with the deposit number CGMCC No. 32521 and the deposit date November 6, 2024. The address of the deposit institution is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; Escherichia coli ( Escherichia coli )BL21 star (DE3) BLBRTXST3 is used in the fermentation production of lactose-N-neotetraose (LNnT). The present invention improves the yield of lactose-N-neotetraose, uses a chromatographic simulated moving bed to produce high-purity lactose-N-neotetraose with a purity greater than 94%, replaces the traditional solvent crystallization method, has simple separation and purification, is conducive to industrial production, and simultaneously produces linear lactose-N-neohexaose with a purity greater than 95%.

The invention relates to a traditional Chinese medicine composition with functions of tonifying qi and nourishing blood for livestock. The traditional Chinese medicine composition comprises the following components in parts by weight: 10-30 parts of astragalus membranaceus, 10-40 parts of spina gleditsiae, 10-20 parts of angelica sinensis, 10-20 parts of codonopsis pilosula, 10-30 parts of uniflower swisscentaury root, 10-30 parts of ligusticum wallichii, 10-20 parts of cowherb seed, 10-20 parts of fruit of liquidambar formosana hance and 1-10 parts of iron sucrose. The traditional Chinese medicine composition is scientific and reasonable in component, free of toxic and side effects and medicine residue, convenient to take and low in cost, has the functions of tonifying qi and nourishing blood, stimulating the menstrual flow and prompting lactation, and is capable of effectively treating diseases of postpartum hypogalactia, breast milk stoppage and the like which are normal in livestock.

Methods, devices, and systems for concentrating breast milk are described. Concentrating nutrients in breast milk may be achieved using an apparatus comprising at least one forward osmotic membrane. In an example the apparatus can include a first sheet of material comprising a forward osmotic membrane, a second sheet of material sealed to the first sheet of material about a common outer perimeter thereof, the first sheet of material arranged so that water can traverse the forward osmotic membrane to an interior of the first and second sheets, and a dry carbohydrate within the interior of the first and second sheets exhibiting an osmotic draw property to draw water from un-concentrated, expressed human milk to form concentrated human milk. After a predetermined time, or upon the milk remaining after drawing of water through the membrane being a desired amount, the water draw can end.

A breast pump device is disclosed. The breast pump device includes a primary machine portion, and a secondary control portion provided separately from the primary machine portion. The primary machine portion further includes a main housing, a pump attached to the main housing and configured to generate a pressure to cause breast milk to be expressed by a user of the breast pump device, and a milk container coupled to the pump and configured to accommodate the breast milk. The secondary control portion is electrically coupled to the primary machine portion without an airway and configured to remotely operate the primary machine portion.

The invention discloses infant formula milk powder rich in breast milk oligosaccharide and having an anti-inflammatory effect and a preparation method of the infant formula milk powder rich in breast milk oligosaccharide, the infant formula milk powder rich in breast milk oligosaccharide and having the anti-inflammatory effect comprises LNB and LNT II, the content of the LNB is 2.5 g / 130g of milk powder, the content of the LNT II is 2.5 g / 130g of milk powder, or the infant formula milk powder rich in breast milk oligosaccharide and having the anti-inflammatory effect comprises the LNT II, the content of the LNT II is 5g / 130g of milk powder. The anti-inflammatory effect is relatively good, and the intestinal flora and the intestinal barrier can be regulated.

The invention belongs to the technical field of microbial engineering, discloses a composition for promoting infant brain development and application thereof, and provides a composition containing lactobacillus paracasei N1115, breast milk oligosaccharide and whey protein powder, the composition can promote infant brain and nerve development, all the components have a synergistic effect, and the effect of promoting infant brain and nerve development is achieved. The conversion from latent TGF-beta in the whey protein powder to activated TGF-beta is activated, so that the aims of promoting neurodevelopment, improving memory and promoting brain development of infants are fulfilled. The preparation method is suitable for preparing milk powder, cheese, yoghourt, plant-based yoghourt and derivative products or other foods for promoting brain development of infants.

The invention relates to the technical field of detection of breast milk oligosaccharides, and provides a method for detecting breast milk oligosaccharides by ion exchange chromatography tandem mass spectrometry, which comprises the following steps: detecting a breast milk sample by ion exchange chromatography tandem mass spectrometry to obtain at least 42 breast milk oligosaccharides. According to the technical scheme, the problem that high-throughput detection of breast milk oligosaccharide is difficult to realize in related technologies is solved.

The present invention provides special medical use formulae useful for enhancing and / or improving the social behavior of an individual. The formula food for the special medical purpose comprises DHA (docosahexaenoic acid), ARA (arachidonic acid) and breast milk oligosaccharide 6-SL and / or LNnT, and the mass ratio of the DHA to the ARA to the breast milk oligosaccharide 6-SL and / or LNnT is (70-210): (60-420): (10-400). The special medical use formulae of the present invention contribute to enhancing and / or improving the social behavior of an individual.

The invention belongs to the technical field of biology, and particularly relates to bifidobacterium longum subsp.infantis FMBL B250020GXY for metabolizing breast milk oligosaccharide and application of the bifidobacterium longum subsp.infantis FMBL B250020GXY, the bifidobacterium longum subsp.infantis FMBL B250020GXY is preserved in China Center for Type Culture Collection on September 1, 2025, and the preservation number is CCTCC M 20251931; three kinds of breast milk oligosaccharides, namely 2 '-fucosyllactose (2'-FL), lactose-N-neotetraose (LNnT) and lactose-N-tetraose (LNT), can be metabolized; plant source carbohydrates such as fructo-oligosaccharide and synanthrin can be metabolized; the compound has a relatively strong capability of removing DPPH and ABTS free radicals; a good inhibition effect is achieved on common pathogenic bacteria; good tolerance to acid and cholate is achieved; the strain is sensitive to gentamicin, streptomycin, tetracycline, chloramphenicol and vancomycin; the compound can be used for preparing antioxidant products, medicines for inhibiting pathogenic bacteria, infant foods and fermented foods, and has a wide application prospect.

The invention belongs to the technical field of nutritional compositions, and discloses a breast milk oligosaccharide-containing composition for promoting brain metabolism and relieving diarrhea and application of the breast milk oligosaccharide-containing composition, and the breast milk oligosaccharide-containing composition contains breast milk oligosaccharide consisting of 2 '-fucosyllactose, 3'-sialic acid lactose and 6 '-sialic acid lactose in a specific weight ratio. The composition disclosed by the invention is used for preparing products with the functions of promoting brain development or metabolism and relieving diarrhea. The composition prepared by the invention can be used for preparing formula milk powder, yoghourt and various metabiotics preparations with corresponding effects.

The utility model belongs to the technical field of breast milk equipment, and discloses a breast pump which comprises a main body and a main machine, the main machine is communicated with the main body and used for generating negative pressure in the main body, the main body is provided with a protective cover, the breast pump further comprises a heating assembly, the heating assembly is arranged on the main machine and electrically connected with the main machine, and the heating assembly comprises a heating face. The heating surface can be in contact with the inner wall of the protective cover to heat the protective cover, in the using process, the inner wall of the protective cover is firstly heated by the heating assembly, and then milk sucking is started, so that an additional heating system is avoided, the cost is saved, waste is avoided, the portability of a product is improved, and the size of the product is reduced.

1. Name of the Design Product: Wearable Hot Compress Breast Pump (W16). 2. Use of the Design Product: It is used in the field of breast pumps to help parturient women extract breast milk. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.