691 results about "Breast milk" patented technology

The invention discloses bifidobacterium animalis subsp. Lactis for relieving ulcerative colitis and constipation and application of the bifidobacterium animalis subsp. Lactis, and belongs to the field of microorganisms. According to the invention, a strain of bifidobacterium anaticum subsp. Lactis LIHUO 01 is screened out, the strain is preserved in the Guangdong Microbial Culture Collection Center on November 20, 2024, and the preservation number is GDMCC No: 65512. The invention also discloses a preparation method of the bifidobacterium anaticum subsp. Lactis strain, and the bifidobacterium anaticum subsp. Lactis strain has the advantages that the bifidobacterium anaticum subsp. Lactis strain can be used for preparing the bifidobacterium anaticum subsp. Lactis strain; the bifidobacterium animalis subsp. Lactis LIHUO 01 has good in-vitro probiotic characteristics, has the effects of relieving ulcerative colitis, improving constipation and regulating intestinal flora, and has a good application prospect in preparation of food or health care products or medicines for relieving ulcerative colitis or improving constipation or regulating intestinal flora.

The invention relates to application of breast milk oligosaccharides to improvement of intestinal flora of mothers and infants, in particular to application of 2 '-fucosyllactose to preparation of food which is taken by a mother and contributes to regulation of intestinal flora of the mother and offspring thereof, and the mother is in a gestation period and / or a lactation period. The invention is provided through a large amount of research; when a mother supplements a certain amount of 2 '-fucosyllactose in a gestation period and / or a lactation period, the abundance of beneficial bacteria in intestinal tracts of the mother can be remarkably improved, the content of harmful bacteria is reduced, meanwhile, the abundance of beneficial bacteria in intestinal tracts of filial generations of the mother is remarkably improved, and the content of harmful bacteria is reduced; particularly, the abundance of some beneficial bacteria which cannot be directly eaten in the intestinal tracts of the mother and the offspring can be adjusted.

1. The name of the product of this design: Breast Pump (S07). 2. Purpose of the product of this design: to assist lactating women in extracting breast milk. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The invention belongs to the technical field of biology, and provides a porcine epidemic diarrhea virus mRNA vaccine and application. The mRNA molecule comprises a coding region for coding the S protein of the PEDV; the PEDV is of a G2a type, and two proline mutations I1076P and L1077P are introduced into an S protein. The invention further discloses a DNA molecule. An S protein of PEDV; a recombinant plasmid; recombining the host cell; an mRNA vaccine formulation which can be used to prepare an antibody against the S protein of PEDV after immunizing an animal; the invention further discloses application of the mRNA molecule, the DNA molecule, the recombinant plasmid or the host cell to preparation of a medicine for preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The vaccine prepared by the invention is fast in antibody generation and high in protection rate, can generate high-level neutralizing antibodies in serum and breast milk of sows after the pregnant sows are immunized before parturition, has a remarkable protection effect on piglets, and can effectively prevent the piglets from being infected with PEDV (Porcine Epidemic Diarrhea Virus).

The present invention relates to a nutritional composition, food and pharmaceutical products comprising the nutritional composition, and uses thereof. The nutritional composition comprises medium and long chain fatty acid triglycerides and breast milk oligosaccharides, and the weight ratio of the medium and long chain fatty acid triglycerides to the breast milk oligosaccharides is 1: 1 to 9: 1. The nutritional composition disclosed by the invention can play a role in synergistically promoting skeletal development and particularly improving bone mineral density.

The invention belongs to the field of food, and particularly relates to a nutritional composition containing probiotics and breast milk oligosaccharide for improving anxiety or depression and application of the nutritional composition. The invention provides application of a nutritional composition in preparation of food beneficial to improving constipation and anxiety state and / or depression state caused by constipation. Wherein the nutritional composition comprises the following components (i) and (ii): (i) a bifidobacterium probiotic, and (ii) a sialylated breast milk oligosaccharide; the bifidobacterium probiotics at least comprise animal bifidobacterium subsp. Lactis, the sialylated breast milk oligosaccharide at least comprises 6 '-sialylated lactose, and the animal bifidobacterium subsp. Lactis comprises a Bb-12 strain. The substance combination of the bifidobacterium probiotics and the sialylated breast milk oligosaccharide can synergistically improve abnormal brain-intestine interaction, and improve constipation and anxiety and / or depression state caused by constipation.

A breast milk-derived Limosilactobacillus fermentum MSJK0025 capable of improving the intestinal flora condition and the use thereof. The breast milk-derived Limosilactobacillus fermentum MSJK0025 is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No. 26426. The breast milk-derived Limosilactobacillus fermentum is a new strain isolated from breast milk of healthy puerperae, and is referred to as Limosilactobacillus fermentum MSJK0025. The strain has a good antibiotic sensitivity and stable inheritance of traits, has a good tolerance to acid, bile salts and artificial gastric / intestinal fluid, and has a fast growth rate and good safety. Furthermore, the strain has a strong intestinal colonization capability and can effectively inhibit intestinal pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Candida albicans, but has no inhibitory effects on intestinal probiotics such as Lactobacillus and Bifidobacterium.

The invention provides a breast milk source exosome as well as a preparation method and application thereof. The preparation method of the breast milk source exosome comprises the following steps: pre-treating breast milk, and then carrying out primary purification concentration and fine purification on the breast milk source exosome after the pre-treatment; wherein the pretreatment comprises the step of mixing degreased breast milk with a sodium phosphate solution; the primary purification concentration comprises tangential flow ultrafiltration treatment; the fine purification comprises purification treatment by adopting a multi-mode chromatography. The invention provides a large-scale purification process of the breast milk exosome, which can be used for treating a large number of samples, improving the purity and yield of the breast milk exosome and ensuring the morphological integrity of the breast milk exosome.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum subsp. Longum as well as a composition, a pharmaceutical composition, a culture, a food product or a dietary supplement containing the bifidobacterium longum subsp. Longum and application of the bifidobacterium longum subsp. Longum. The bifidobacterium longum is preserved in the Guangdong Microbial Culture Collection Center on January 21, 2025, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.65836. The bifidobacterium longum subsp. Longum has relatively strong gastric acid and cholate tolerance and intestinal colonization capability; the bifidobacterium longum subsp. Longum can effectively utilize bifidobacterium bifidum to release monosaccharide or disaccharide outside cells for efficient proliferation, and the composition containing the bifidobacterium longum subsp. Longum, the bifidobacterium bifidum and breast milk oligosaccharide can release a large amount of short-chain fatty acid, so that the effects of maintaining the physiological function of an organism and improving the health of the organism are achieved.

The invention relates to a bifidobacterium longum subsp. Infantis Imu-01 and an application thereof. Specifically, the invention provides bifidobacterium longum subsp.infantis Imu-01, a culture, a preparation and application of the bifidobacterium longum subsp.infantis Imu-01 in preparation of a product for improving the immune function. The bifidobacterium longum subsp. Infantis provided by the invention can regulate and control the immune level of an object, improve the content of butyric acid in intestinal tracts and improve the utilization of oligosaccharide in breast milk.

The invention provides a breast milk feeding amount intelligent calculation and dynamic adjustment method and system, and relates to the technical field of intelligent feeding, and the method comprises the steps: collecting real-time physiological parameters of an infant, constructing an infant digital model through a multi-scale morphological neural network, calculating a basic energy consumption value through combining with a biological rhythm prediction model, and calculating the feeding amount of the infant. The standard breast milk feeding amount is calculated through the self-adaptive fuzzy neural network; the method comprises the following steps: collecting mother physiological state data and breast milk component data, calculating a breast milk nutrition density coefficient by using a deep mixing cognitive network and an immune evolution self-organizing network, correcting a breast milk reference feeding amount, and finally calculating an actual breast milk feeding amount through a self-adaptive resonance theory network; growth and development data of an infant after breast milk intake are collected, a multi-layer spiral pulse neural network is used for extracting development feature vectors, a development deviation value is calculated based on a fractal dynamics model, network parameters are dynamically adjusted through a biological group intelligent optimization system, and dynamic optimization of breast milk feeding amount is achieved.

The invention provides application of lactobacillus rhamnosus B2-1 sourced from breast milk in preparation of products for relieving specific dermatitis, the lactobacillus rhamnosus B2-1 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 8, 2022, and the preservation number is CGMCC NO.26167. The lactobacillus rhamnosus B2-1 has been preserved in the China General Microbiological Culture Collection Center (CGMCC). The lactobacillus rhamnosus B2-1 disclosed by the invention has a good effect of relieving the specific dermatitis, and has potential functional development potential.

The invention belongs to the technical field of nutritious food, and discloses a composition for simulating whole breast milk oligosaccharide and application of the composition. In the composition for simulating the whole breast milk oligosaccharide, the mass ratio of 2 '-fucosyllactose to lactose-N-neotetraose to 3'-sialyllactose is (3.9-8.1): (0.8-1.2): (0.8-1.2), and the breast milk oligosaccharide 2 '-fucosyllactose, the lactose-N-neotetraose and the 3'-sialyllactose are mutually matched to take effect in a synergistic manner, so that the whole breast milk oligosaccharide is obtained. The composition can improve the memory ability and cognitive ability of an eating object, can be added into infant food (including infant formula powder, complementary food and nutritional supplements) and nutritional supplements or food of children over 3 years old, teenagers and aged people, and has a wide application prospect.

A breast pump includes a main body, a breast milk suctioning shield, and one or more air pumps. The breast milk suctioning shield is assembled in an accommodation space of the main body and detachably connected to the main body. A front end of the breast milk suctioning shield has a breast shielding portion, and a nipple passage extends from a rear end of a center portion of the breast shielding portion. One or more deformable members and a non-deformable supporting member are assembled with an annular connection portion located between the breast shielding portion and the nipple passage. The air pump has an intake port and an exhaust port. The intake port is controlled by a first valve controller to suction the nipple passage and generate a negative pressure, and the exhaust port is controlled by a second valve controller to inflate or deflate the deformable member.

The invention belongs to the technical field of food, and relates to application of breast milk oligosaccharide in regulating brain immunity. Specifically, the invention relates to the non-therapeutic purpose of adjusting brain immunity by adjusting polarization of microglial cells M1 / M2, and the breast milk oligosaccharide is any one of 3-fucosyllactose, lactose-N-tetraose, lactose-N-neotetraose, 3 '-sialic acid lactose and 6'-sialic acid lactose. Research results show that a specific variety of breast milk oligosaccharides (especially 3-FL) can obviously inhibit the generation of neurotoxic factors NO, inflammatory factors TNF-alpha and IL-1beta in microglial cells, and promote the generation of anti-inflammatory cytokines IL-10 in the microglial cells, so that the state of the microglial cells is improved, damage repair is promoted, and the microglial cells are prevented from being damaged. Therefore, the effect of regulating brain immunity is achieved.

The invention discloses an application of a breast milk-derived plant lactobacillus in improving allergic diseases and regulating intestinal flora, and belongs to the technical field of microorganisms. The plant lactobacillus GDMCC No: 63060 disclosed by the invention is obtained by being separated from breast milk. The strain provided by the invention has excellent gastrointestinal fluid tolerance, mucin adhesion and antibiotic sensitivity, can inhibit various pathogenic bacteria, can generate indole derivatives, effectively inhibits eczema mouse dermatitis symptoms, inhibits Th2 type immunoreaction hyperpolarization, and induces regulatory T cells. Therefore, the lactobacillus plantarum FN029 has a huge application prospect in preparation of products for inhibiting enteropathogenic bacteria, improving allergic diseases, regulating intestinal flora or promoting digestion function.

The present invention introduces a breast milk collection device with a first shell, a second shell, and a membrane. The first shell has a milk suction passage and a milk accumulation chamber with an opening. The second shell is connected to the first shell and has a fixing seat. The membrane includes a negative pressure diaphragm and an annular one-way valve diaphragm connected to its outer edge. The membrane is adjustable, the negative pressure diaphragm tightly seals the milk suction passage, and the one-way valve diaphragm can block the opening. A negative pressure cavity forms between the negative pressure diaphragm and the second shell. The breast milk collection device features a durable, simple, and easily assembled one-way valve for efficient breast milk collection.

The invention provides a milk powder composition containing breast milk oligosaccharide and easy to digest and absorb. The milk powder composition comprises the breast milk oligosaccharide, OPN, galactooligosaccharide, lactoferrin, alpha-whey protein, multivitamins, lactose, vegetable oil and compound minerals, wherein the weight ratio of the lactoferrin to the OPN to the breast milk oligosaccharide to the galactooligosaccharide is 1: (2-4): (2-50): (4-100). The composition can effectively recover and improve the intestinal barrier function caused by inflammatory factors, can be well absorbed by infants with poor digestion and absorption functions, reduces diarrhea and promotes skeletal development.

The invention belongs to the field of food, and particularly relates to a nutritional composition of oligosaccharide and probiotics and application of the nutritional composition. The invention provides application of a nutritional composition in preparation of food beneficial to improvement of constipation and improvement of cognitive competence decline caused by constipation. Wherein the nutritional composition comprises the following components (i) and (ii): (i) a neutral fucosylated breast milk oligosaccharide, and (ii) a bifidobacterium probiotic; wherein the neutral fucosylated breast milk oligosaccharide at least comprises 2 '-fucosylated lactose, and the bifidobacterium probiotics at least comprise bifidobacterium longum subsp. Longum. According to the present invention, with the substance combination of the neutral fucosylated breast milk oligosaccharide and the bifidobacterium probiotic, the abnormal brain-intestine interaction can be improved, such that the constipation can be easily improved, and the cognitive ability reduction caused by the constipation can be easily improved;

The present application relates to a kind of Bifidobacterium longum infantis subsp.Imu-01 and its application. Specifically, the present application provides Bifidobacterium longum infantis subsp.Imu-01, culture, preparation and preparation of immune function improving product application. The present application provides that Bifidobacterium longum infantis can regulate the immune level of the subject, improve the content of intestinal butyric acid, improve the utilization of human milk oligosaccharide.

The invention relates to recombinant escherichia coli and a method for synthesizing sialic acid lactose-N-tetrasaccharide c, and belongs to the technical field of microbial metabolism engineering. According to the research, engineering escherichia coli BAZ for producing LNTri II is taken as an initial strain, two key glycosyl transferases, namely beta 1, 4-galactosyl transferase and alpha 2, 6-sialyltransferase, and three CMP-Neu5Ac pathway enzymes, namely NeuC, NeuB and NeuA, are introduced, and related competitive genes are knocked out; under the conditions of shake-flask culture and fed batch culture, the LST c yield of 1.718 g / L and the LST c yield of 9.745 g / L are achieved through the engineering bacterium respectively, and the huge potential of a microbial cell factory in the aspect of efficient production of complex breast milk oligosaccharides is shown.

The present invention belongs to the technical field of breast milk secretion detection, and provides a wearable device for dynamically detecting the amount of milk secretion. The present invention includes a multimodal measurement device and a control unit. The multimodal measurement device includes at least two types of electrodes, and the electrodes include an excitation electrode close to the root of the breast and a measuring electrode close to the nipple. The multimodal measurement device also includes a micro-flow measurement mechanism, and at least two fluid channels are provided inside the micro-flow measurement mechanism. One end of the fluid channel is connected to the side of the wearable device close to the nipple, and the other end is connected to the outside of the wearable device. To ensure the uniformity of the current covering the breast, the measuring electrode close to the nipple is close to the measurement target tissue, which directly reflects the impedance changes of the lactation-related tissue; the micro-flow measurement mechanism is used to provide physical parameters independent of the impedance measurement, which can provide a reference standard for the lactation amount measured by impedance, and improve the robustness and accuracy of the lactation measurement system.

A breast milk collection device, comprising an adaptor adapted at a first wide end to receive a woman's breast and a second narrow end of the adaptor communicating with a reservoir. The reservoir is adapted to receive breast milk from the adaptor. The second narrow end of the adaptor has a first aperture and a hollow drip tube in communication with the first aperture. The hollow drip tube has a second aperture through which the hollow drip tube communicates with an internal volume of the reservoir. A one-way valve element communicates with the hollow drip tube and with the internal volume of the reservoir. A flexible barrier is disposed across a hollow chamber formed in a rigid barrier housing, the flexible barrier creating a hermetic seal between the rigid barrier housing and the hollow chamber, and the hollow chamber fluidly communicating with an interior of the hollow drip tube. A drive assembly including a reciprocally driven member contacts a first surface of the flexible barrier to reciprocally drive the flexible barrier to a first position during a first position of the reciprocally driven member. The flexible barrier returns to a second position during a second position of the reciprocally driven member, providing an alternating source of vacuum pressure and relief of vacuum pressure in the hollow drip tube.

The invention discloses a method for rapidly determining the content of breast milk oligosaccharide in dairy products, which comprises the following steps: sampling and pretreating different types of dairy products according to sample states, carrying out ultrasonic extraction, refrigerated centrifugal degreasing and centrifugal ultrafiltration protein purification on the obtained sample, and reducing the purified sample solution by using sodium borohydride to obtain a sample solution to be detected; reducing the breast milk oligosaccharide standard substance by sodium borohydride to obtain a standard solution to be detected; determining the standard substance solution and the to-be-detected sample solution by adopting ultra-high performance liquid chromatography-tandem mass spectrometry, establishing a standard curve, and carrying out quantitative analysis on the to-be-detected sample solution; the chromatographic conditions are as follows: an ACQUITY UPLC BEH Amide hydrophilic interaction chromatographic column is adopted; the mobile phase A is 20 mmol / L ammonium formate; the mobile phase B is acetonitrile; and gradient elution.

A method and application of Escherichia coli BL21star (DE3) BLBRTXST3 and its fermentation method for producing high-purity lactose-N-neotetraose, the invention relates to the technical field of human milk oligosaccharide production, Escherichia coli )BL21 star (DE3) BLBRTXST3 is deposited in the General Microbiology Center of China Microorganism Culture Collection Committee, with the deposit number CGMCC No. 32521 and the deposit date November 6, 2024. The address of the deposit institution is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; Escherichia coli ( Escherichia coli )BL21 star (DE3) BLBRTXST3 is used in the fermentation production of lactose-N-neotetraose (LNnT). The present invention improves the yield of lactose-N-neotetraose, uses a chromatographic simulated moving bed to produce high-purity lactose-N-neotetraose with a purity greater than 94%, replaces the traditional solvent crystallization method, has simple separation and purification, is conducive to industrial production, and simultaneously produces linear lactose-N-neohexaose with a purity greater than 95%.

The invention relates to a traditional Chinese medicine composition with functions of tonifying qi and nourishing blood for livestock. The traditional Chinese medicine composition comprises the following components in parts by weight: 10-30 parts of astragalus membranaceus, 10-40 parts of spina gleditsiae, 10-20 parts of angelica sinensis, 10-20 parts of codonopsis pilosula, 10-30 parts of uniflower swisscentaury root, 10-30 parts of ligusticum wallichii, 10-20 parts of cowherb seed, 10-20 parts of fruit of liquidambar formosana hance and 1-10 parts of iron sucrose. The traditional Chinese medicine composition is scientific and reasonable in component, free of toxic and side effects and medicine residue, convenient to take and low in cost, has the functions of tonifying qi and nourishing blood, stimulating the menstrual flow and prompting lactation, and is capable of effectively treating diseases of postpartum hypogalactia, breast milk stoppage and the like which are normal in livestock.

The present invention relates to a composition comprising human milk oligosaccharides and probiotics, its application, and milk powder and microbial preparations comprising the same. The human milk oligosaccharide is lactose-N-trisaccharide LNT-II, and the probiotic is animal bifidobacterium. The combined use of LNT II with animal bifidobacterium at a specific concentration of the present invention can improve the competitive adhesion ability of animal bifidobacterium lactis subspecies relative to pathogenic bacteria, thereby achieving the effect of competitive inhibition of pathogenic bacteria.