56 results about "Antibiotic sensitivity" patented technology

The method includes several steps including obtaining a bacterial sample; identifying the type of bacteria in the bacterial sample; selecting a set of antibiotics based on the identity of the bacteria in the bacterial sample; obtaining a control sample from the bacterial sample; placing the bacterial sample in solutions containing the set of antibiotics; determining concentration of bacteria in the respective antibiotic solutions; determining growth curves for the respective antibiotic solutions based on the determined bacterial concentration; and comparing the growth curves for the respective antibiotic solutions with a growth curve determined from the control sample. An identification and quantification system may be used to select the set of antibiotics, and further may be used in the steps of determining concentration of bacteria in the respective antibiotic solutions and determining growth curves for the respective antibiotic solutions based on the determined bacterial concentration.

The present invention includes compositions and methods of bacteriophage to increase antibiotic sensitivity in bacteria. In one aspect, the invention includes a method of increasing antibiotic sensitivity in multi-drug resistant (MDR) bacteria. Another aspect includes a pharmaceutical composition comprising a lytic bacteriophage. Yet another aspect includes a method of treating a multi-drug resistant bacterial infection in a subject. Yet another aspect includes a method of disrupting a pathogenic bacteria associated with a biofilm and compositions for use thereof.

An apparatus for inspecting an antibiotic and a method for determining antibiotic sensitivity using the same is provided. The antibiotic susceptibility inspection time which has conventionally taken longer than 24 hours is shortened to about 2 hours or less, the efficacy of the target substance is monitored in real time, the identification of the microorganism, the kind of the antibiotic capable of treating the microorganism, and the minimum dosage thereof are quickly confirmed. Microbial infections requiring prompt diagnosis and treatment can be effectively treated.

A gene transfer method for the agrobacterium of poplar (Xingjiang poplar) includes explant selection, culture medium screening, hormone addition, antibiotic sensitivity and culture conditions. It can be used for selectively culturing the new stres-resistance variety of Xingjiang poplar within shorter time.

This invention is a diagnostic plate that can be used to select antibiotic combinations with efficacy against microorganisms growing as a biofilm. The plate allows growth of biofilm on a plurality of projections, and the subsequent simultaneous challenge of biofilms on all projections of the plate to independent concentrations and combinations of anti-biofilm agents. Resistance of microorganisms to antibiotics is higher when they grow as a biofilm, as compared to when they grow in a planktonic state which is usually used to determine their level of antibiotic sensitivity. Growth of microorganisms that slough off the biofilm in the anti-biofilm agent challenge determines the Minimum Inhibitory Concentration (MIC) which relates to sensitivity of the microorganisms in a planktonic state. Growth of any surviving microorganisms from the biofilm in a subsequent recovery step determines the Minimal Biofilm Eradication Concentration (MBEC) which relates to the sensitivity of the microorganisms growing as a biofilm. Enumeration of the surviving microorganisms in the recovery step determines the Minimum Biocidal Concentration (MBC).

The preparation process of cow mammitis staphylococcus culture fluid and its use belongs to the field of animal epidemic disease diagnosing, preventing and treating technology. The preparation process includes dissolving peptone 10 g, yeast powder 5 g, sodium pyruvate 10 g, glycin 12 g and lithium chloride 5 g in water of 980 ml; regulating pH to 7.3 with sodium hydroxide; sterilizing at 121 deg.c; cooling to 50+/-10 deg.c; adding bacteria-free 1% concentration potassium tellurite water solution in 10 ml and Tween-80 in 7 ml, and preservation at 4 deg. c. The culture fluid may be used in the fast identification of cow mammitis staphylococcus and antibiotic sensitivity test.

The preparation process of cow mammitis streptococcus culture fluid and its use belongs to the field of animal epidemic disease diagnosing, preventing and treating technology. The preparation process includes dissolving peptone 10 g, yeast powder 5 g, sodium chloride 10 g, glucose 5 g and sodium azide 0.5 g in water of 1 L; regulating pH to 7.2 with sodium hydroxide; adding bacteria-free 0.1% concentration crystal violet water solution in 1.3ml and 1% concentration bromocreasol purple water solution in 1.5ml; sterilizing at 121deg.c and preservation at 4 deg. c. The culture fluid may be used in the fast identification of cow mammitis streptococcus and antibiotic sensitivity test.

This invention is a diagnostic plate that can be used to select antibiotic combinations with efficacy against microorganisms growing as a biofilm. The plate allows growth of biofilm on a plurality of projections, and the subsequent simultaneous challenge of biofilms on all projections of the plate to independent concentrations and combinations of anti-biofilm agents. Resistance of microorganisms to antibiotics is higher when they grow as a biofilm, as compared to when they grow in a planktonic state which is usually used to determine their level of antibiotic sensitivity. Growth of microorganims that slough off the biofilm in the anti-biofilm agent challenge determines the Minimum Inhibitory Concentration (MIC) which relates to sensitivity of the microorganisms in a planktonic state. Growth of any surviving microorganims from the biofilm in a subsequent recovery step determines the Minimal Biofilm Eradication Concentration (MBEC) which relates to the sensitivity of the microorganisms growing as a biofilm. Enumeration of the surviving microorganims in the recovery step determines the Minimum Biocidal Concentration (MBC).

The invention discloses application of pithecellobium clypearia extracts to preparation of multi-drug resistant acinetobacter baumannii medicine. The pithecellobium clypearia extracts are prepared through the method includes the steps that pithecellobium clypearia coarse powder is extracted with water or an ethyl alcohol solution, the obtained extraction liquid is extracted with ethyl acetate, and the obtained extracts are target products. The antibacterial function of the pithecellobium clypearia extracts to multi-drug resistant acinetobacter baumannii and the sensitivity enhancing function of the pithecellobium clypearia extracts to similar antibiotics are disclosed for the first time. Tests prove that the pithecellobium clypearia extracts and imipenem or tetracycline or polymyxin B or ceftazidime or levofloxacin take effect together, and the use amount of antibiotics can be reduced by 50-87% compared with the mode that only the pithecellobium clypearia extracts are used. The pithecellobium clypearia extracts can serve as natural multi-drug resistant acinetobacter baumannii medicine or an antibiotic sensitivity-enhancing agent, and is applied to treatment of diseases caused by acinetobacter baumannii. A new means and alternative medicine are provided for solving the drug resistance problem of similar antibiotics.

The invention belongs to the technical field of genetic engineering breeding, and in particular relates to a breeding method using protocorm based on germinated phalaenopsis seeds as a receptor. The method comprises the following steps of obtaining the protocorm, culturing in multiplication, pre-culturing, dip-dyeing agrobacterium, co-culturing, eluting the agrobacterium, screening, culturing, forming shoots and seedlings, and rooting. A set of phalaenopsis transgenosis receptor system is constructed by using the protocorm from the germinated phalaenopsis seeds as a receptor material, and meanwhile, a set of genetic transformation system based on agrobacterium dip-dyeing and a corresponding detection system are established, the receptor material can be fast obtained, the performance is stable, a source is wide, and the breeding method is good to selective antibiotic sensitivity, and the problem that the phalaenopsis is insensitive to antibiotic in the induction type protocorm process, a resistance transgenosis material can be conveniently screened, a good and pure new phalaenopsis system can be obtained, and the breeding method has the good popularization applicable value on prompting the new variety development of the phalaenopsis.

The invention belongs to the technical field of medicine and particularly discloses application of L-arginine in improvement of antibiotics sensitivity of bacteria. According to the application, through improving a proton motive force of the bacteria by the L-arginine, the quantity of antibiotics entering interior of the bacteria can be increased, and finally, the bacteria die, so that the L-arginine can be used for improving the sensitivity of the bacteria to the antibiotics, and thus, the problem of bacterial drug resistance is solved. Through jointly using the L-arginine and the antibiotics, the bactericidal action of the antibiotics can be remarkably improved, and the effect is better and the safety and operability are higher compared with that in the prior art that only the antibiotics serve as an antibacterial drug.

The preparation process of cow mammitis E. coli culture fluid and its use belongs to the field of animal epidemic disease diagnosing, preventing and treating technology. The preparation process includes dissolving peptone 10 g, No. 3 bile salt 1.5 g, lactose 10 g, sodium chloride 5 g and sodium citrate 5 g in water of 1000ml; regulating pH to 7.2 with sodium hydroxide; adding bacteria-free 1% concentration crystal violet water solution in 0.5 ml and 0.4% concentration phenol red water solution in 4 ml; sterilizing at 121 deg.c and preservation at 4 deg. c. The culture fluid may be used in the fast identification of cow mammitis E. coli and antibiotic sensitivity test.

The invention discloses a method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli. The method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli comprises the following steps of (1) performing strain recovery; (2) screening riemerella anatipestifer and escherichia coli with antibiotics sensitivity differences; (3) inoculating the riemerella anatipestifer and the escherichia coli into a culture medium for mixed culture at a certain time interval or different diluting concentration; (4) measuring the minimum inhibitory concentration of antibiotics in the second step on the screened riemerella anatipestifer and escherichia coli; (5) preparing an antibiotic plate culture medium; (6) separating mixed culture bacterium liquid. The method for mixed culture and re-separation of riemerella anatipestifer and escherichia coli has the advantages that two kinds of bacteria are used outside duck poultry bodies and can jointly grow for more than 20 generations; conditions are provided for studying drug resistance transfer and relevant mechanisms of the two kinds of bacteria under the symbiosis condition; important significance is realized on disease prevention and control of duck breeding industry.

Described is a method for identifying a gene which mediates antibiotic sensitivity or resistance in a target bacterium, the method comprising the steps of: (a) generating a pool of mutant target bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises an outward-facing promoter (TnAP) capable of increasing transcription of a gene at or near its insertion site in the DNA of said target cells; (b) generating a control microdroplet library by encapsulating individual members of the pool of step (a) in microdroplets, the microdroplets comprising a volume of aqueous growth media suspended in an immiscible carrier liquid, each microdroplet comprising a single mutant target cell; (c)generating a test microdroplet library by encapsulating individual members of the pool of step (a) in microdroplets, the microdroplets comprising a volume of aqueous growth media containing the antibiotic and suspended in an immiscible carrier liquid, each microdroplet comprising a single mutant target cell; (d) incubating the control and test microdroplet libraries to produce control and test microcultures; and (e) comparing the distribution of TnA insertions between control and test microcultures to identify a gene which mediates antibiotic sensitivity or resistance in said target bacterium.

The invention discloses a whole genome sequencing analysis method of pigling diarrhoea Escherichia coli, and belongs to the technical field of biological gene detection. The whole genome sequencing analysis method of pigling diarrhoea Escherichia coli comprises following steps: sample collecting; separation and identification of fecal sample Escherichia coli; serotype identification; virulence factor result detection; whole genome sequencing analysis and whole genome sequence study of Escherichia coli containing a plurality of virulence factors. According to the whole genome sequencing analysis method, whole genome sequencing is adopted for more comprehensive knowing of the genome information of Escherichia coli ST4214, and analysis of the virulence factors contained by Escherichia coli ST4214; genome analysis of pigling diarrhoea Escherichia coli ST4214 possesses significant importance on study of pigling diarrhoea epidemic serotype; study on pigling diarrhoea Escherichia coli ST4214 drug resistance gene is capable of guiding applications of antibiotics based on the antibiotic sensitivity and drug resistance, and avoiding antibiotics abuse; and the whole genome sequencing analysis method is used for providing data base for development of pigling diarrhoea vaccines.

A method for creating the gene transfer receptor system of lily includes disinfecting the surface of lily bulb or filament, cutting the become segments, culturing in differentiating culture medium to create a high-frequency regeneration system, antibiotic sensitivity test to determine the screening concentrations of cephamycin, hygromycin and kanamycin, inducing the rooting adventitious embryo, and transplanting.

The invention discloses a preparation method of salix suchowensis resistant seedlings. The preparation method comprises preparation and pre-culture of explants; preparation of an infective bacterium solution; infection; co-culture; bacterium washing; differentiation and screening culture till visual kanamycin resistant buds are differentiated; continuous culture of the kanamycin resistant buds in an adventitious bud elongation selection medium; separation of each kanamycin resistant bud when the kanamycin resistant buds are 1 to 2 cm long; culture of each independent kanamycin resistant bud in a rooting selection medium; subculture for a certain time, so as to obtain the integral resistant seedlings. The preparation method is used for preparing the salix suchowensis resistant seedlings from the aspects of explant selection, culture medium optimization, hormone adjustment, antibiotic sensitivity, culture condition and the like, thereby providing a new way for tree gene functional research.

The present invention provides methods and kits for performing microbial sensitivity tests. Specifically, the invention relates to methods for identifying the susceptibility, tolerance or resistance of a microorganism to one or more antimicrobial agents, and the level of tolerance. The method comprises exposing the microorganism grown on a plate surface to one or more antimicrobial agents and to at least one growth promoting agent.

The invention provides an adhesive protein sourced from Bifidobacterium longum. An amino acid sequence of the protein is determined, on the basis, experiments find that the adhesive protein not only can adhere to an enterocyte, but also has a function of improving antibiotics sensibility of microorganisms, and the antibiotics synergy on Salmonella typhimurium is particularly prominent. On the basis of the beneficial discovery, an application of the protein as an anti-bacterial synergist for Salmonella typhimurium is determined, an application range of the protein is extended, and a new path for improving the drug sensitivity of the microorganisms is provided. Prominent technological effects are obtained on the basis of strict experimental measures, and broad application prospect is provided.

The present invention discloses lactic acid bacteria having ampicillin resistance, and preparation and applications of a preparation of the lactic acid bacteria having ampicillin resistance. According to the present invention, human manure is separated under antibiotic pressure to obtain two strains of lactic acid bacteria have drug resistance, an antibiotic sensitivity test is performed, and whether plasmid DNA exists is detected; PCR amplification is performed to analyze the drug resistance gene localization conditions of the strains and explore the molecular mechanisms of the antibiotic resistance of the lactic acid bacteria, and safety evaluation is performed on the screened lactic acid bacteria having the resistance gene; whether the screened resistance strain provides an adjustment effect on intestinal tract imbalance is verified, and an antibiotic type intestinal tract flora imbalance model is used so as to provide potential treatment methods for diarrhea, flora imbalance and other diseases caused by the use of antibiotics in the clinic; and according to the obtained strain of the present invention, the intestinal tract probiotics can survive under the antibiotic pressure when the antibiotic is clinically used again while the ordinary probiotics can not survive, such that the treatment method can be provided for the diarrhea caused by the antibiotics in the clinic.

The invention discloses a method for identifying bacteria in a biological sample and a method for testing antibiotic sensitivity of the bacteria in the biological sample. The method for identifying the bacteria in the biological sample comprises the following steps: (1) centrifugally separating the bacteria in the biological sample; (2) dyeing the bacteria obtained in the step (1); (3) fixing the bacteria dyed in the step (2) through an electrostatic adsorption effect; (4) after an extraction solution is injected into the tip of a capillary tube, extracting metabolite of single live bacteria in an in-situ extraction manner; and (5) inserting an electrode into the capillary tube obtained in the step (4), applying voltage, and carrying out mass spectrometry. The method disclosed by the invention does not need to depend on incubation, can realize bacterial species identification and antibiotic sensitivity test in an initial bacterial infection biological sample, has the advantages of rapidness, high sensitivity, non-targeted analysis and the like, and has a good application prospect.