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Application of pithecellobium clypearia extracts to preparation of multi-drug resistant acinetobacter baumannii medicine

A multi-drug resistant Acinetobacter baumannii technology, applied in antibacterial drugs, plant/algae/fungal/moss ingredients, pharmaceutical formulations, etc. Allergy etc.

Active Publication Date: 2016-08-03
HUACHENG PHARMA FACTORY GAUNGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN103385912A discloses that the extract of monkey earrings has anti-MRSA effect and antibiotic sensitization effect, but the patent does not mention the antibacterial effect of the monkey earrings extract on multi-drug resistant Acinetobacter baumannii and the increase in combination with antibiotics. Sensitization

Method used

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  • Application of pithecellobium clypearia extracts to preparation of multi-drug resistant acinetobacter baumannii medicine
  • Application of pithecellobium clypearia extracts to preparation of multi-drug resistant acinetobacter baumannii medicine
  • Application of pithecellobium clypearia extracts to preparation of multi-drug resistant acinetobacter baumannii medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation method of monkey earring extract

[0024] Monkey earrings (Pithecellobiumclypearia Benth) were provided by Guangzhou Huacheng Pharmaceutical Factory. Take an appropriate amount of monkey earring medicinal material, grind it into coarse powder, reflux twice with water or ethanol for 2 hours each time, and filter; combine the filtrates and concentrate to obtain an extract (water or ethanol extract). Suspend the extract with water, extract with ethyl acetate three times, combine the ethyl acetate extracts, and concentrate to obtain the ethyl acetate extract. The ethanol extract can be prepared by refluxing 10-95% ethanol.

Embodiment 2

[0026] Bacteriostatic and bactericidal tests of monkey earring water extract against multidrug-resistant Acinetobacter baumannii and the sensitizing effects of combination with imipenem, tetracycline, polymyxin B, ceftazidime and levofloxacin respectively.

[0027] 1. Experimental method

[0028] 1) Determination of minimum inhibitory concentration (MIC):

[0029] Water extract of monkey earrings, imipenem (IMP), tetracycline (TE), polymyxin B (POLB), ceftazidime (CAZ), levofloxacin (LVX) were carried out in a series of multiple ratios in MH broth medium Dilute, 50μl per well, adjust the inoculum to 1.0×10 6 CFU / ml, add 50 μl bacterial solution to each well. Incubate at 35°C; for 24 hours, the lowest concentration of antibacterial drug without precipitation is its minimum inhibitory concentration (MIC).

[0030] 2) Determination of minimum bactericidal concentration (MBC):

[0031] Using the plate coating counting method, draw 50ul of bacterial suspension from the aseptic ...

Embodiment 3

[0077] Antibacterial and bactericidal test of 10% ethanol extract from monkey earrings against multi-drug resistant Acinetobacter baumannii and investigation on the sensitizing effects of combination with imipenem, tetracycline, polymyxin B, ceftazidime and levofloxacin respectively.

[0078] By the experimental method under embodiment 2, measure the minimum inhibitory concentration (MIC) of monkey earring 10% ethanol extract, minimum bactericidal concentration (MBC), and imipenem, tetracycline, polymyxin B, ceftazidime and The minimum inhibitory concentration (MIC) of levofloxacin combined drug, and evaluate the effect of combined drug.

[0079] 2. Experimental results

[0080] Table 13 shows the in vitro antibacterial test results of 10% ethanol extract of monkey earrings and five antibiotics (imipenem, tetracycline, polymyxin B, ceftazidime, and levofloxacin) against multidrug-resistant Acinetobacter baumannii.

[0081] See Table 14 for the in vitro bactericidal test resul...

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Abstract

The invention discloses application of pithecellobium clypearia extracts to preparation of multi-drug resistant acinetobacter baumannii medicine. The pithecellobium clypearia extracts are prepared through the method includes the steps that pithecellobium clypearia coarse powder is extracted with water or an ethyl alcohol solution, the obtained extraction liquid is extracted with ethyl acetate, and the obtained extracts are target products. The antibacterial function of the pithecellobium clypearia extracts to multi-drug resistant acinetobacter baumannii and the sensitivity enhancing function of the pithecellobium clypearia extracts to similar antibiotics are disclosed for the first time. Tests prove that the pithecellobium clypearia extracts and imipenem or tetracycline or polymyxin B or ceftazidime or levofloxacin take effect together, and the use amount of antibiotics can be reduced by 50-87% compared with the mode that only the pithecellobium clypearia extracts are used. The pithecellobium clypearia extracts can serve as natural multi-drug resistant acinetobacter baumannii medicine or an antibiotic sensitivity-enhancing agent, and is applied to treatment of diseases caused by acinetobacter baumannii. A new means and alternative medicine are provided for solving the drug resistance problem of similar antibiotics.

Description

technical field [0001] The invention relates to a new application of the monkey earring extract, in particular to its application in the preparation of anti-multi-drug-resistant bacteria drugs. Background technique [0002] The 21st century is the era of multi-drug-resistant bacteria. After 60 years of clinical application of antibiotics, more and more nosocomial infections and multi-drug-resistant bacterial infections have become a major problem in clinical antibacterial treatment. In 1961, Jerons reported the first clinical case of A-resistant Patients with methicillin Staphylococcus aureus (MRSA) infection, currently MRSA infection has gradually spread all over the world; the distribution of main drug-resistant bacteria in China's bacterial resistance monitoring clinic in 2011, Escherichia coli, Klebsiella pneumoniae producing extended-spectrum β -Lactamase (ESBL) strains averaged 50.7% and 38.5% respectively, and the change and status quo of their drug resistance have at...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P31/04
CPCA61K36/48A61K2236/53A61K2236/333A61K2236/331
Inventor 李沛波周倩刘翀苏薇薇王永刚彭维
Owner HUACHENG PHARMA FACTORY GAUNGZHOU
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