53 results about "Minimum bactericidal concentration" patented technology

The invention discloses a method for measuring plant pathogenic fungus inhibitory activity of lotus leaf total alkaloids and application of the plant pathogenic fungus inhibitory activity. The method includes: preparing spore suspension; preparing the lotus leaf total alkaloids; measuring the inhibitory effect of the lotus leaf total alkaloids; measuring the minimum bactericidal concentration of the lotus leaf total alkaloids; measuring the minimum inhibitory concentration of the lotus leaf total alkaloids. The method has the advantages that the fact that the lotus leaf total alkaloids have evident plant pathogenic fungus inhibitory activity is determined for the first time, the lotus leaf total alkaloids have the highest inhibitory activity on cucumber wilt fusarium, and the minimum bactericidal concentration and minimum inhibitory concentration of the lotus leaf total alkaloids are 160 microgram / mL; due to the fact that the lotus leaf resources in China are rich, extraction of the active alkaloids from the lotus leaves is low in cost, nontoxic and harmless; the lotus leaf total alkaloids are used for preventing and treating plant diseases, can be used as potential biological pesticide, and is promising in development and application prospect.

The invention discloses a deciquam and iodine compound disinfectant, which comprises the components by weight percent to prepare an intermediate product: 1.00-4.50 percent of iodine; 0.01-0.10 percent of potassium iodide, 30-50 percent of deciquam; 15-30 percent of surfactant; the balance of solubilizer; the intermediate product is obtained, and then water is added into the intermediate product for dilution so as to obtain the final product. The disinfectant has disinfection efficiency upon the bacteria, and the escherichia coli 8099 is taken as an example and the deciquam solution and the povidone iodine solution are taken as the comparison to conduct a sterilizing experiment to achieve the same disinfection efficiency (sterilizing rate is equal to or greater than 99.9 percent), the minimum concentration of deciquam and iodine of the invention is 25mg / L and 1,25mg / L respectively; the minimum concentration of deciquam in the deciquam solution is 63mg / L, and the minimum concentration of iodine in povidone iodine is 2.5mg / L, and the result indicates that the minimum sterilizing concentration of deciquam and iodine is remarkably reduced. The porcine parvovirus virus is taken as an example to indicate the disinfection efficiency upon the virus of the disinfectant, and the result indicates that the inactivation ratio of the disinfectant reaches over 99.9 percent, while the deciquam solution cannot kill the porcine parvovirus virus. The experiment indicates that iodine and deciquam of the compound disinfectant are subjected to complexing to form an entirety under the action of the surface active agent and the like, and the compound disinfectant has a strong synergistic effect.

The invention constructs procaryon recombinant plasmid of mouse beta-alexin 3 gene (Mbd-3) by gene engineering technology, comprising the steps of transferring the procaryon recombinant plasmid into engineering bacteria for induction efficient expression of mouse beta-alexin 3 polypeptide (MBD-3), extracting and purifying expression product and exonuclease to release active MBD-3 mature peptide. The invention detects the minimum inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of recombinant mouse beta-alexin 3 for resisting different mycelial fungus by mcroscale broth dilution method, proves that the recombinant mouse beta-alexin 3 polypeptide has function of resisting pathogenicity mycelial fungus so as to invent a novel medicament for curing mycotic infection.

The invention provides a preparation method of a trametes tomentosa fermentation liquor ethyl acetate extract. The preparation method comprises the following steps: filtering out mycelia in trametes tomentosa fermentation liquor, extracting the mycelia for 4-6 times by using dichloromethane and methanol which are isovolumetrically mixed as an extraction solvent, combining extract solutions, and concentrating to obtain a mycelium extract; and carrying out vacuum concentration on the fermentation liquor after filtering out the mycelia to obtain concentrated fermentation liquor, mixing the mycelium extract with the concentrated fermentation liquor, extracting for 6-8 times by using ethyl acetate, merging the extract liquor, and carrying out vacuum concentration to obtain the trametes tomentosa fermentation liquor ethyl acetate extract. The trametes tomentosa ethyl acetate extract disclosed by the invention is highly sensitive to staphylococcus aureus and strong in bacteriostatic activity, the minimum bactericidal concentration of the trametes tomentosa ethyl acetate extract to staphylococcus aureus is 0.0975-0.04875 mg / mL, and the trametes tomentosa ethyl acetate extract can be used as a novel natural staphylococcus aureus bacteriostatic agent.

The invention discloses the application of vanillic acid in inhibiting the growth of multidrug-resistant Enterobacter cloacae. The invention utilizes the disc diffusion method to determine the drug resistance of Enterobacter cloacae to antibiotics, and determines that the minimum bactericidal concentration of vanillic acid to multidrug-resistant Enterobacter cloacae is 1.6mg / mL and the minimum pH The bacterial concentration was 1.2 mg / mL, revealing that the non-antibiotic compound vanillic acid has an inhibitory effect on multidrug-resistant Enterobacter cloacae, and has wide application value in the field of medicine.

The invention discloses applications of deoxyschizandrin in inhibiting growth of multi-drug-resistance escherichia coli. Deoxyschizandrin has good in-vitro killing action on human multi-drug-resistance Escherichia coli resisting ampicillin, nalidixic acid, cefotaxime, ciprofloxacin, cefoxitin and the like, the deoxyschizandrin can inhibit the growth of multi-drug-resistance escherichia coli at theminimum bactericidal concentration of 25.57 microg / mL and minimum inhibitory concentration of 3.13 microg / mL. The invention provides the inhibition of deoxyschizandrin on human multi-drug-resistanceescherichia coli. The deoxyschizandrin can effectively relieve or solve the drug-resistance infection problem of the multi-drug-resistance escherichia coli, reduce the case fatality rate, thus havingimportant practical significance on providing a new concept for inhibiting the human multi-drug-resistance Escherichia coli.

The invention discloses an application of chelerythrine to restraining growth of staphylococcus lugdunensis. The chelerythrine has better in vitro destroying effects on medicine-resistant staphylococcus lugdunensis and can restrain the growth of the medicine-resistant staphylococcus lugdunensis, the minimum bactericidal concentration is 16 [mu]g / mL, and the minimal inhibition concentration is 7.8[mu]g / mL. The invention provides the restraining effects of the chelerythrine on the staphylococcus lugdunensis, and the chelerythrine has wide application value in the field of pharmaceuticals and thelike.

The present invention provides blue colored aqueous dispersion of silver nanoparticles and process for preparation thereof. The present invention also provides compositions comprising blue colored dispersion of silver nanoparticles. The blue colored aqueous dispersion comprising silver nanoparticles wherein dispersion is characterized by —having plasmonic peaks in the range 330-335 nm and 650-720 nm combined with missing plasmonic peaks in the range 390 to 410 nm and 410-500 nm in UV-Vis spectrum, —the dispersion having silver nanoparticles of anisotropic shape with majority of particles (>65%) having equivalent diameter in range of 0.5 to 6 nm, —Molar extinction coefficient greater than 10.1 preferably in the range of 10.1 to 15.7 mM−1 cm−1 at wavelength of maximum absorption in the range of 650-720 nm, —Dispersion stability of at least 15 months, —Minimum Bactericidal Concentration (MBC) lower than 0.10 ppm preferably in the range of 0.055-0.099 ppm.