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Preparation method and application of trametes tomentosa fermentation liquor ethyl acetate extract

A kind of technology of ethyl acetate extraction, Trametes versicolor, applied in the field of preparation of Trametes versicolor extract

Pending Publication Date: 2021-10-29
宁夏师范学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there is no relevant report on the inhibition of Staphylococcus aureus by the extract of Trametes versicolor fermentation broth. Research Work of Ethyl Ester Extract as Staphylococcus Aureus Bacteriostatic Agent

Method used

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Embodiment Construction

[0021] The preparation and antibacterial activity of the ethyl acetate extract of Trametes tomentosa fermentation broth of the present invention will be further described by specific examples below.

[0022] (1) Select the fruiting body of Trametes versicolor that is tender, robust and plump, without pests and diseases. Under aseptic conditions, wipe and disinfect the surface of the fruiting body of Trametes versicolor with 75% alcohol, then rinse it with sterile water, and absorb it with a sterile cloth. Dry, use a scalpel or a razor blade to cut the mushroom block into about 1cm, and break the fruiting body into two halves with both hands. Leave half of the fruiting bodies in the left hand, and hold the inoculation knife in the right hand. After burning and cooling, pick a piece of tissue the size of a rice grain from the middle of the bacteria block, quickly transfer it to the center of the PDA medium, and invert the plate to room temperature for 3-5 days. When the hyphae c...

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PUM

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Abstract

The invention provides a preparation method of a trametes tomentosa fermentation liquor ethyl acetate extract. The preparation method comprises the following steps: filtering out mycelia in trametes tomentosa fermentation liquor, extracting the mycelia for 4-6 times by using dichloromethane and methanol which are isovolumetrically mixed as an extraction solvent, combining extract solutions, and concentrating to obtain a mycelium extract; and carrying out vacuum concentration on the fermentation liquor after filtering out the mycelia to obtain concentrated fermentation liquor, mixing the mycelium extract with the concentrated fermentation liquor, extracting for 6-8 times by using ethyl acetate, merging the extract liquor, and carrying out vacuum concentration to obtain the trametes tomentosa fermentation liquor ethyl acetate extract. The trametes tomentosa ethyl acetate extract disclosed by the invention is highly sensitive to staphylococcus aureus and strong in bacteriostatic activity, the minimum bactericidal concentration of the trametes tomentosa ethyl acetate extract to staphylococcus aureus is 0.0975-0.04875 mg / mL, and the trametes tomentosa ethyl acetate extract can be used as a novel natural staphylococcus aureus bacteriostatic agent.

Description

technical field [0001] The present invention relates to the preparation of a Trametes versicolor extract, in particular to a preparation method for the ethyl acetate extract of the Trametes versicolor fermentation broth, and the present invention also relates to the ethyl acetate extract of the Trametes versicolor fermentation broth as grape aureus Application of bacteriostatic agents. Background technique [0002] Staphylococcus aureus ( Staphylococcus aureus ) is a common food-borne pathogen, which often causes suppurative infections in humans and animals, which not only seriously threatens food hygiene and safety, but also has serious hazards in animal production. In order to reduce the harm caused by Staphylococcus aureus, various synthetic antibacterial agents are widely used in food and other aspects. While preventing infection, the application of various antibacterial agents has also brought new problems to human health. In recent years, people's demand for natural ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01N63/50A01P1/00A61K36/07A61P31/04C12N1/14C12R1/645
CPCA01N63/30A01N63/50A61K36/07A61P31/04C12N1/14
Inventor 丁建海权晨曦刘世巍范箫艺兰嘉豪禹建芳陈徐回刘晓敏魏婕程子芥梅瀚
Owner 宁夏师范学院
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